The growth behaviour of the transfected cells in hygromycin selection media - (Nov/11/2006 )
please help, this is my first time! i have transfected my chinese hamster cells derived from V79 with lipofectamine. After doing a kill curve for non transfected cells i chose a dose of 600 microgram per ml of hygromycin for selection of the transfected cells. Now, should i expect transfected colonies, which are suppose to be hygromycin resistant look healthy and grow with normal speed from day one in the selection culture, comparing to the non transfected colonies, or should i expect a slower growth for the transfected cells?
-abyasemani-
QUOTE (abyasemani @ Nov 11 2006, 11:57 AM)
please help, this is my first time! i have transfected my chinese hamster cells derived from V79 with lipofectamine. After doing a kill curve for non transfected cells i chose a dose of 600 microgram per ml of hygromycin for selection of the transfected cells. Now, should i expect transfected colonies, which are suppose to be hygromycin resistant look healthy and grow with normal speed from day one in the selection culture, comparing to the non transfected colonies, or should i expect a slower growth for the transfected cells?
I would not expect anything.....
To my experience, it depends on the cell type and the vector and the protein the cell expressing... I once expressed a "toxic" protein in fibroblast with hygromycin selection, the cells grow very slowly... however, I have a control cell line, which expressed the "empty" hygromycin vector, growing as normal as normal cells.
hope that helps
-yeping-