Selection of cell ine - (Feb/23/2003 )
Dear all, I would like to conduct a project on breast cancer but I dont know how to select cell line. Would you please give me some guidlines to choose for one to work as model system? Thanks.
Sorry, I would like to add that the project is about screening of drugs.
(1) Select your cell line or your tartget kinase (protein).
(2) Set up a in vitro and/or cell based assay using recombinant protein or whole cells.
(3) select your functional readout for detection e.g. fluorescence in kinase, caspase...or cell death index for apoptosis- MTT, JC-1 dye....
(4) Screen the compound library for inhibiting target kinase or activating caspase or killing cells.