cells and media - (Mar/01/2007 )
Dear all,
I am a beginner in tissue culture work. I have not done anything with tissue culture before, so I have no idea of it.
I am going to order tissue culture media for my works. However, I found that for a specific media, there are several types of different media. For example, DMEM alone has different types with variation in the content of a few elements (according to the Sigma's catalogue).
My question is, how do I know which one of them suit the cell-line I am going to work with? For your information, the cell-lines that I am going to work with are Vero cells, 293T cells and HeLa cells.
Thank you in advance for your advices.
Cheers,
Virus_fan
-virus_fan-
QUOTE (virus_fan @ Mar 1 2007, 07:02 AM)
Dear all,
I am a beginner in tissue culture work. I have not done anything with tissue culture before, so I have no idea of it.
I am going to order tissue culture media for my works. However, I found that for a specific media, there are several types of different media. For example, DMEM alone has different types with variation in the content of a few elements (according to the Sigma's catalogue).
My question is, how do I know which one of them suit the cell-line I am going to work with? For your information, the cell-lines that I am going to work with are Vero cells, 293T cells and HeLa cells.
Thank you in advance for your advices.
Cheers,
Virus_fan
I am a beginner in tissue culture work. I have not done anything with tissue culture before, so I have no idea of it.
I am going to order tissue culture media for my works. However, I found that for a specific media, there are several types of different media. For example, DMEM alone has different types with variation in the content of a few elements (according to the Sigma's catalogue).
My question is, how do I know which one of them suit the cell-line I am going to work with? For your information, the cell-lines that I am going to work with are Vero cells, 293T cells and HeLa cells.
Thank you in advance for your advices.
Cheers,
Virus_fan
See ATCC collection site. Input your cell line and there you can find appropriate media for cell culture
ATCC
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