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  1. cell lysate colorless? - (reply: 1)
  2. How to prevent cell aggregation - (reply: 1)
  3. Problem with CD2F1 mouse CT26 cell cachexia protocol. - CT26 treated mice gained weight. (reply: 2)
  4. can i use antibiotics with lipofectamine - my cells have antibiotic as selective marker and need neomycin (reply: 4)
  5. Sp2/o Myeloma cell line - Media for growing sp2/0-Ag 14 cell line (reply: 4)
  6. Apoptosis by FACS - How to gate the cells by using Apoptosis kit from BD (reply: 1)
  7. Should i use cells healed from mycoplasma - (reply: 2)
  8. What's the major cell type in thymus - (reply: 1)
  9. How to change medium without dislodging the cells - (reply: 1)
  10. Cell Lysis - (reply: 2)
  11. Serum Deprivation - MDA-MB-231 cells - (reply: 5)
  12. VEGF secretion in MDA-MB-231 cell line - (reply: 3)
  13. THP-1 cells and 2-mercaptoethanol - (reply: 1)
  14. Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
  15. cell and tissue culture - healthy test (reply: 1)
  16. Need human cells for a science fair project - (reply: 4)
  17. What kind of cells is it? - need which counterstain? (reply: 1)
  18. Cell pellet after freezing - (reply: 3)
  19. cell culture maintainance - (reply: 3)
  20. pls recommend a serum independent expression promoter for mammalian cells - (reply: 1)
  21. Spontaneous Cell lysis after IPTG induction - (reply: 4)
  22. Treating cells with cytokines - Should you take out growth factors before treating? (reply: 2)
  23. Ways to stop the cell at G1 phase? - (reply: 2)
  24. Immunstaining cells fixed in 10% formalin- possible? - (reply: 8)
  25. difference between transformed and immortalized cells - (reply: 3)
  26. How many O.D. cells to load onto SDS-PAGE? - (reply: 2)
  27. Troubles in IL-8 measurement of tissue cells cultured with E.coli - (reply: 1)
  28. Mioblasts primary cell culture - counting the number of myoblasts (reply: 1)
  29. RNA extraction from frozen cells suspended in PBS - (reply: 2)
  30. Cell Clumping Problem - Cells Stick to Eachother and Not the Flask (reply: 2)
  31. Linear DNA transfection - Daudi cells (reply: 3)
  32. Dead cell staining - what kind of dye? (reply: 5)
  33. What exactly are CD4+3- inducer cells? - simply a specific form of "T-helper-cells"? (reply: 2)
  34. In vitro toxicology test by animal cell line - Looking for methods (reply: 1)
  35. inoculating liquid media from frozen cell stock ok? - (reply: 6)
  36. Cell treating - Do you... (reply: 4)
  37. cell lysis - (reply: 3)
  38. How to fixate cells onto glass microscope slide - (reply: 2)
  39. Is there any destructive effect on nucleic acid when harvest the cell by scrapin - (reply: 1)
  40. Transfected copies per cell - (reply: 1)
  41. RAW 264 cells - (reply: 1)
  42. How to concentrate retrovirus produced by Phoenix-Ampho cell - (reply: 4)
  43. Have I killed my cells? Doh! - (reply: 3)
  44. Looking for specific cancer cell line - Need a parent line with metastatic and non-metastatic lines (reply: 2)
  45. Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
  46. mesenchymal stem cell differentiation into osteoblasts (alizarin) - (reply: 2)
  47. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
  48. siRNA into RAW264.7 cells? - any ideas (reply: 4)
  49. Heat-shock treatment problems with adherent cells - (reply: 1)
  50. No Retrovirus using the Phoenix - Ampho cells - (reply: 1)
  51. IEC-6, IL-6 and LPS - Cannot get my IEC-6 cells to secrete Il-6 (reply: 2)
  52. Creating stable cell lines - (reply: 4)
  53. Cholesterol Assay - Commerically available cholesterol assay kit for cell extracts (reply: 1)
  54. Can Osteosarcoma cells change their phenotype? - previously "Name this cancer cell's purpose and parts" (reply: 1)
  55. Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (reply: 13)
  56. cell culture question - passage number - (reply: 5)
  57. A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
  58. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
  59. 293t Cell Attachment Issues - (reply: 7)
  60. giant cells? - (reply: 6)
  61. Direct Cell Lysis in Laemmli - (reply: 1)
  62. Cell Morphology - (reply: 1)
  63. When to use PMSF in a cell lysis buffer? - (reply: 9)
  64. staining for receptors in cell line - (reply: 1)
  65. Counting B cells in hemacytometer - (reply: 2)
  66. Coating cell dishes with peptides - (reply: 2)
  67. Does it look like cell clumps/artefact/contamination? - (reply: 3)
  68. What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
  69. what cell line to use for visualizing membrane localized protein - (reply: 1)
  70. Define cell passage number - (reply: 2)
  71. THP-1 cells from Sigma - (reply: 1)
  72. DNA "snot" in cell lysis/protein extraction - (reply: 6)
  73. DNA extraction from dry cells - How to extract DNA from dry cells (reply: 9)
  74. H.influenzae and S.pneumoniae cell culture - H.influenzae and S.pneumoniae grow on plates but not in broth (reply: 1)
  75. Cells die after changing medium - Is there role of FBS? (reply: 1)
  76. Different cell culture plastics, problems with cell adherance - (reply: 6)
  77. Cytotoxicity assay with RAW 264.6 cells - (reply: 1)
  78. Decide which cell line to use - (reply: 3)
  79. Cell seeding density - (reply: 11)
  80. 293T cells adhesion and transfection... - Looking for cell line for localization studies... (reply: 3)
  81. Cell culture supernatant - Is it just the media from cultured cells? (reply: 1)
  82. growth curve? - how to establish growth curve of specific cell line.... (reply: 1)
  83. transfecting human lymphoblastoid cells - (reply: 2)
  84. H9 Cell transfection - H9 cell transfection protocol (reply: 4)
  85. MCF-7 drug synergy - (reply: 4)
  86. How to kill bacteria in treated cells? - (reply: 6)
  87. cell line to use for neural regeneration? - (reply: 1)
  88. Does immortalization affect non-dividing short-living cells? - (reply: 3)
  89. Why do viable cells take up DAPI? - DAPI must only stain non-viable cells (ICC) (reply: 7)
  90. Quenching oxygen in cell culture - (reply: 1)
  91. Inhibitors in cell culture... - DMSO???? (reply: 4)
  92. % Cytotoxicity or Relative Cell Damage less than zero - What does it imply? (reply: 4)
  93. Ratio between cells and lysis buffer - (reply: 1)
  94. Immunofluorescense of floating cells - How to IF the floating cells during apoptosis? (reply: 4)
  95. What cell lines can be used to run an anti-angiogenic assays? - (reply: 1)
  96. NK Cell Isolation - negative selection kits - (reply: 1)
  97. Cell count in solution - (reply: 2)
  98. Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
  99. Cell Culture protocol by Current Protocol - free online version from invitrogen (reply: 3)
  100. cell cryopreservation in -80 - (reply: 2)
  101. Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 33)
  102. Cell growth inhibition by lipofectamine 2000 - (reply: 1)
  103. T cell activation for intracellular cytokine detection by Flow Cytometry - (reply: 1)
  104. Stem Cell Niche - Murine Adult hematopoietic stem cell division Niche?? (reply: 1)
  105. spinner flasks cell maintenance - (reply: 3)
  106. contamination of DH5alpha cell line - (reply: 6)
  107. MDCK cell experience - (reply: 2)
  108. Expression of EGFR in MDA-MB-435 cell line - (reply: 2)
  109. how to distinguish monocyte and dendritic cell under microscope - (reply: 2)
  110. cell cultur medium - (reply: 3)
  111. High-throughput screening of suspension cell lines - (reply: 2)
  112. Single Bacterial Cell Isolation for Comet Assay - (reply: 1)
  113. FACS data analysis - understanding cell quest program and reading data from it (reply: 1)
  114. Problems with cell lysis - (reply: 3)
  115. Causes for vacuoles or bubbles in cultured cells - (reply: 7)
  116. Differences in MCF-7's? - (reply: 2)
  117. what are the methods available for protein extraction from cell line? - (reply: 6)
  118. Cell Counting in Hemocytometer - Teach me short cut to count in this chamber (reply: 13)
  119. Best method to locate a receptor on breast cancer cell? - Using an AFM (reply: 2)
  120. the latest research trend in dendritic cells - the heat research topics related to dendritic cells (reply: 1)
  121. T cell proliferation assay with macrophages - (reply: 2)
  122. Problems when harvesting cells - (reply: 4)
  123. About red precipitate during passing the cell line - (reply: 2)
  124. housekeeping gene for B cell lines - (reply: 1)
  125. Who uses non-adherent cells? - like NCI-H69... are they ready to use after passing? (reply: 1)
  126. no pellet after centrifugation for making competent cells - (reply: 4)
  127. Daudi cells transfection - (reply: 1)
  128. Primary tracheal cell culture - (reply: 1)
  129. Need some advice on HeLa cells health hazards - (reply: 7)
  130. Reviving THP1 cells - (reply: 6)
  131. Protein Isolation of very low cell number... - (reply: 4)
  132. making INV110 competent cells - (reply: 1)
  133. b-mercaptoethanol and stem cells - (reply: 3)
  134. Cell fractionation problem - Nucleaus forms a pellet that can't be suspended (reply: 5)
  135. Bradford problem in cell lysates - (reply: 1)
  136. Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
  137. Medium for culturing 2 types of cell together - (reply: 2)
  138. Transfect competent cell? - (reply: 1)
  139. Need advice on counting low cell numbers - (reply: 1)
  140. Can I load 1N NaOH in cell culture plate? - 1N NaOH is strong alkaline, will it eat up the glass? (reply: 4)
  141. Why 10% serum for cell culture? - (reply: 11)
  142. MCF-7 & MTT assay & E-screen - (reply: 1)
  143. flourescence in non-transfected cells! - (reply: 4)
  144. % confluency question - when to transfect the cells? (reply: 2)
  145. Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
  146. Free grid image for counting cells (freeware) - simple but useful (reply: 7)
  147. Question on cell line - (reply: 1)
  148. T98G cell size - Coulter counter (reply: 1)
  149. TOP10 competent cell with another vector - (reply: 1)
  150. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
  151. siRNA for retinal pigment epithelial cells - (reply: 1)
  152. high levles of cell death in PC12 cells - (reply: 3)
  153. PMA - used to differentiate THP-1 cells - (reply: 6)
  154. Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
  155. tranwells and C6 glioblastoma cell migration - (reply: 1)
  156. Cell culture contamination - (reply: 9)
  157. yeast cell cycle and temperature - FACS profile at 25 and 36 (reply: 1)
  158. Melanin extraction from melanoma cells, How? - (reply: 3)
  159. Strange black particles while growing Sf9 insect cells - (reply: 3)
  160. I thawed my primary cells and froze them again? serious mistake? - cell culture (reply: 5)
  161. FRET Imaging :Hela cells - (reply: 2)
  162. Jurkat Cells and Proliferation Assay - Click-iT EdU - (reply: 2)
  163. cell density - (reply: 2)
  164. 3-D tissue model for endothelial cell culture - Looking for any in vitro 3-D models to grow endothelial cells to measu (reply: 1)
  165. How to kill yeast cells :) - chloramine or autoclaving (reply: 19)
  166. RNA isolation from small amount of suspension cells - (reply: 3)
  167. Poor ligation efficiency using Invitrogen TOP10 and XLI-blue cells - (reply: 5)
  168. Primary gastric epithelial cells - (reply: 1)
  169. Ca++ estimation in the cell - (reply: 1)
  170. picking a cell line to perform cell transformation assays - (reply: 2)
  171. cell differentiation - (reply: 2)
  172. Culture of Epithelial cells from Breast Milk? - (reply: 2)
  173. HEP G2 cells - growth medium - (reply: 2)
  174. poor cell growth following FBS batch change - (reply: 5)
  175. CHO cells medium - (reply: 5)
  176. Paradoxical cell count - Increase in cell count wihtout (known) stimuli (reply: 2)
  177. Can the current technology make a human cell solely using materials from nature? - Can the current technology make a human cell solely using materials fr (reply: 2)
  178. Could we grwo chinese hamster cells in untreated normal petri dishes? - (reply: 1)
  179. Is stem cell ethical? - Do you support or oppose it? (reply: 2)
  180. ES cell targeting - (reply: 2)
  181. vehicle treated cells? - (reply: 2)
  182. siRNA transfection in endothelial cells - (reply: 3)
  183. How to confirm whether the cell line was successfully transfected with two plasm - Co-transfection of two plasmids to 293 cells (reply: 6)
  184. how to test the efficiency of chemically competent cells - (reply: 2)
  185. gene down-regulated in cancer cell line but no proliferation inhibit ability - (reply: 3)
  186. sudden cell death- Please HELP - (reply: 6)
  187. cell adhesion problems in outer wells for transfeciton - (reply: 1)
  188. cell lysis buffer - (reply: 4)
  189. RNA isolation from small amounts of cells - (reply: 1)
  190. DNA extraction from PFA fixed cells - (reply: 2)
  191. Help of establishing stable cell line - (reply: 1)
  192. Cell lysis method for running cell lysate on Western blot - (reply: 4)
  193. Problems with making efficient chemically competent cells - (reply: 2)
  194. N in Cell Culture - What is the proper way to determine your "n"? (reply: 2)
  195. Can cell debris move? - (reply: 4)
  196. MLN cell isolation....Help! - (reply: 1)
  197. Pansorbin cells - (reply: 3)
  198. double transfection plus siRNA - in HEK293 cells (reply: 1)
  199. cell line culture - (reply: 2)
  200. Chromosomal aberration assay for ES cells - (reply: 1)
  201. Unexplained Cell Culture Death - Expertise needed (reply: 7)
  202. Protocols for Preparation of cell lysate from tissue sample - (reply: 3)
  203. Cell passage question - (reply: 4)
  204. Calcium Flux by FACS using Fluo-4 - Can the cells be fixed? (reply: 1)
  205. Cell lysate at RT - (reply: 4)
  206. Monocyte derived Dendritic cells - (reply: 4)
  207. what is the media for HUVEC cells? - (reply: 4)
  208. Confluent 25 cm2 flask means how many cells? - quick question, need quick answer (reply: 1)
  209. THP-1 serum free cells - (reply: 3)
  210. Can enzyme kinetics be measured in whole cell extracts? - (reply: 3)
  211. cell scraping - I DONT recommend it for subculturing (reply: 15)
  212. what kind of contamination is this? (photos inside) - contamination in cell culture with HeLa (reply: 10)
  213. cell lysate PAGE & blotting problem? - (reply: 1)
  214. Question about nontreated cell cultureware - (reply: 5)
  215. looking for HCV positive cell lines? - (reply: 6)
  216. what would happen if i dilute protease inhibitor too much in cell lysate? - (reply: 3)
  217. A problem about infecting primary T cells - (reply: 1)
  218. what to do with the differentiated mouse es cells - (reply: 3)
  219. What is the pI of most of proteins in cell? - (reply: 3)
  220. easy & quick apoptosis assay for siRNA treated cell line sample - (reply: 3)
  221. Loading cell pellet in SDS-PAGE - (reply: 4)
  222. What happen to my NIH3T3 cells? - (reply: 6)
  223. perovskite oxide nanowires in living cells - (reply: 1)
  224. Really, isn't there anyone who can recommend a TUNEL assay? - for adherent cells using fluorescent microscopy? (reply: 2)
  225. Fixing cells in wells? - (reply: 4)
  226. Problem with thawing cells from liquid nitrogen? - (reply: 6)
  227. Problem with mouse embryonic stem cells - contamination? - (reply: 1)
  228. Cell immortalize - (reply: 2)
  229. TUNEL assay- should I grow cells on slides? - Or label cells in suspension then deposit on slides? (reply: 1)
  230. confirming how the gene causes decreased growth/increased cell death - (reply: 4)
  231. To keep dead cells when harvesting? - (reply: 2)
  232. anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
  233. how to sdudy the matter exchanges among cells? - (reply: 4)
  234. RAW 264.7 cell transfection - Probs transfecting RAW cells with lipofectamine LTX (reply: 2)
  235. Transfecting P19 embryonic carcinoma cells - (reply: 2)
  236. Transfecting NS0 cells - (reply: 5)
  237. Calculating cell purity after MACS enrichment - (reply: 1)
  238. In vivo T cell depletion - (reply: 1)
  239. HEK cell, EGFP and PI staning - (reply: 7)
  240. ligation transformation.......which chemically competent cells are the best? - (reply: 3)
  241. irradiated 3T3 cells as feeder cells - (reply: 2)
  242. Is it true that a cell line cannot be infected with virus again once it was alre - virus infection (reply: 1)
  243. Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
  244. Where can we purchase human microvascular endothelial cells? - (reply: 1)
  245. stimulation of cell division/mitosis? - of non-dividing exocrine cells (reply: 4)
  246. How to make Matrigel layer as thin as humanly possible? - Our technique relies on a VERY close contact of cell with coverslip (reply: 5)
  247. Maintaining stable cell line - (reply: 5)
  248. Stable cell line - clone vs. population - (reply: 15)
  249. How to isolate gastric parietal cells? - Do you have protocol for isolation gastric parietal cell? (reply: 3)
  250. how to distinguish normal and cancerous osteoblast cells - (reply: 3)
  251. How to kill proliferating cells but not arresting cells? - (reply: 3)
  252. Cell contamination - Small rapidly moving black rods (reply: 18)
  253. TMK 1 Cell - Where can I buy TMK 1 cells? (reply: 1)
  254. Agent to punch hole in cell membranes - (reply: 1)
  255. Yeast Competent Cells - (reply: 34)
  256. cell lysate for immunoprecipitation - (reply: 2)
  257. Transient Transfection of HeLa cells. - (reply: 1)
  258. Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
  259. reviving corneal cell line - (reply: 1)
  260. Calculating cells/well in a 96-well plate - Calculating cells/well in a 96-well plate (reply: 8)
  261. ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
  262. Human dendritic cell line - (reply: 5)
  263. Cells dying upon temperature shift. - (reply: 7)
  264. Spermatogonia stem cell culture - (reply: 4)
  265. THP-1 cell culture problem: Cells die after thawing - (reply: 4)
  266. Fixation of cells for long time storage - (reply: 1)
  267. cell migration, cell invasion and cell proliferation assay - (reply: 5)
  268. cell culture - problem with suspension CHO cell revival (reply: 2)
  269. Optimization of seeding cells number for MTT assay - (reply: 5)
  270. MCF-7 cell grow too slow - (reply: 5)
  271. Living cell GFP imaging - (reply: 6)
  272. weird S2 cells - (reply: 1)
  273. neuronal cell suspension from brain - (reply: 4)
  274. Transfection of Drosophila cell culture - Which is the best transfecting agent? (reply: 1)
  275. A CO2 independent medium limit the range of cell types that can be investigated? - (reply: 1)
  276. Centrifugation speed for adherent cell line - (reply: 11)
  277. time required for cell recovery after thawing - (reply: 8)
  278. Competent Listeria Cells - (reply: 3)
  279. Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
  280. Protein extraction from animal cells - (reply: 7)
  281. Sepration of cells from cell debris in sample - (reply: 2)
  282. Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
  283. HBSS vs PBS to deliver compounds to cells - (reply: 3)
  284. centrifugation speed for fixed cell - (reply: 2)
  285. competent cell contamination? - (reply: 3)
  286. Lysis of fat cells/adipocytes - (reply: 7)
  287. CHO-S cell - (reply: 1)
  288. cell lines - (reply: 2)
  289. More deep ChIPping woes (Sonication Optimization) - Primary cell line.... (reply: 1)
  290. how many cells in a mouse spleen - (reply: 5)
  291. The BEST cell counting technique? - (reply: 4)
  292. cell harvesting; use of antibiotics? - (reply: 8)
  293. Best Guess:How many cells in Organotypic Slice culture - (reply: 2)
  294. What are the negative effects of HEPES on cells - if any - (reply: 1)
  295. HELP: transfection reagent for BJ cells - (reply: 1)
  296. Search transfection reagent for BJ cells - (reply: 2)
  297. Why cells are so resistant to G418? - G418 cannot kill cells (reply: 6)
  298. what's wrong with my MTT assay on a suspension cell line - (reply: 8)
  299. Ooops! I messed up! Help! - freezing cells (reply: 5)
  300. Co-IP in sonicated whole cell lysates - (reply: 3)
  301. no GFP in cells - (reply: 7)
  302. SLOW cell growth - (reply: 2)
  303. Apyrase in cell culture - Half-life? (reply: 3)
  304. Cells are lost after fixation - (reply: 14)
  305. primary sertoli cell transfection - (reply: 2)
  306. how to separate dead from viable cells in cultures of non-adherent cells - (reply: 3)
  307. Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
  308. Hela cell contamination? - (reply: 10)
  309. Cat No of M199 medium for endothelial cells URGENT - (reply: 1)
  310. brdu staining on gfp+ve cells - (reply: 1)
  311. How to infect T cells? Lentiviral or retroviral? - (reply: 2)
  312. dendritic cells culture - (reply: 3)
  313. cell lysis and save the ECM - (reply: 1)
  314. Harvesting cells following transfection - Scrapping or trypsin (reply: 2)
  315. Competent cells not growing - (reply: 5)
  316. preparation of bacterial cell lysate - how to do? (reply: 1)
  317. Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (reply: 3)
  318. Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
  319. Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
  320. Cell fractioning in order to perform binding assay - (reply: 1)
  321. competent cell didn't grow in LB - (reply: 15)
  322. BrDU staining on GFP positive cells - (reply: 1)
  323. Contamination or not? - Cell Culture Contamination (reply: 1)
  324. protocol for Trypan Blue exclusion assay - How to do trypan blue exclusiona ssay on adherant cells? (reply: 3)
  325. Infection of human cell lines grown in suspension? - Infection protocols (reply: 3)
  326. Need advice for growing THP-1 cells - (reply: 4)
  327. Live cell nuclei immunostaining - (reply: 2)
  328. Does a B lymphocyte contain lysosome inside? - Cell Structure (reply: 1)
  329. Cell fractionation-help! - (reply: 6)
  330. Nystatin makes vero cells round, too high concentration? - (reply: 3)
  331. How to separate cells in urine for FISH? - (reply: 1)
  332. CaCl2 Competent Cell - (reply: 3)
  333. culturing trouble making competent cells - (reply: 1)
  334. How to pick cell from soft agar and recover it? - (reply: 2)
  335. Schock freezing - cell disruption? - (reply: 3)
  336. Culturing of MUTZ-3 cells - (reply: 1)
  337. Adipocytes from stem cells? - (reply: 6)
  338. culture CD34+ Hematopoietic Stem Cells - (reply: 2)
  339. Preparing cell lysate from cell culture for Western blot - (reply: 4)
  340. How to introduce DNA to animal cells? - (reply: 3)
  341. MTT assay + suspension cells - (reply: 4)
  342. Western Blot of cells in Laemmil - (reply: 5)
  343. AML cell lines - Where can I get them?? (reply: 1)
  344. prepare electrocompetent cells - (reply: 4)
  345. CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
  346. plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
  347. Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
  348. Just how many cells are lost when changing tubes? - (reply: 5)
  349. Cell cycle: ModFIT vs. FlowJo - diferent percentages outcomes on same histograms (reply: 1)
  350. problem with plating adherent cells in 24-well plates - (reply: 11)
  351. Raji Cell Histone Immunocytochemistry and Confocal Help - (reply: 1)
  352. Cell Seeding and Drug treatment - (reply: 12)
  353. Embryonic stem cell culture - (reply: 4)
  354. cell biology video clips - (reply: 2)
  355. cell seeding confusion - what does it mean (reply: 1)
  356. Using Adenovirus to transfect cells - (reply: 5)
  357. Cell assay for ROS measurment - (reply: 1)
  358. Red Cells in Clear Ficoll Layer Following Density Centrifugation - Monocyte Isolation (reply: 2)
  359. preparation of electroporation competent cells from chemically competent cells - (reply: 3)
  360. cell culture - passage number (reply: 7)
  361. do the competen cells chang the U to T - (reply: 4)
  362. increased BrDU incorporation but similiar cell cycle profile by PI or DAPI - (reply: 2)
  363. E.coli TOP10 comp cells T1 Phage resistant? - (reply: 1)
  364. what time is better to seed cells in well or dishes before transfection? - (reply: 7)
  365. Experiment with KATO III cells - (reply: 1)
  366. SiRNA in MIN6 cells, nucleofection - (reply: 1)
  367. Cell passage number for non-cancerous cells - (reply: 2)
  368. Cell death with DMSO treatment, during transfection using polybrene - (reply: 2)
  369. stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
  370. Homework question - what happens to cells when they're put in distilled wate - (reply: 3)
  371. looking for protocol using DAPI for cell cycle analysis - (reply: 1)
  372. Synchronization of NIH 3T3 cells - (reply: 2)
  373. difference between staining vital cell and dead - (reply: 9)
  374. why should the conversion efficiency of competent cell be at least 10^8 cfu/_ - (reply: 2)
  375. Enlarging Cells - (reply: 3)
  376. Stable cell line losing expression faster than usual - (reply: 6)
  377. PCR from cell culture - to detect the presence of adenovirus (reply: 2)
  378. Passaging stable transfected cells after thawing - (reply: 4)
  379. Cell culture - (reply: 2)
  380. Is that possible to establish a stable cell line which is transfected by an empt - (reply: 2)
  381. Could competent cell be stored in liquid nitrogen? - (reply: 7)
  382. what happens if cells turn liquid? - (reply: 4)
  383. Cell Fixation method - % of PFA ? (reply: 4)
  384. culture cell - (reply: 1)
  385. Storing Induced E.Coli Cells - (reply: 1)
  386. choosing competent cells - (reply: 5)
  387. FACS sorting and culturing cells afterwards - (reply: 1)
  388. Secrets for declumping cell pellets - (reply: 5)
  389. Best compotent cells - (reply: 5)
  390. mouse U6 promotor in human ES cells - (reply: 1)
  391. Cell Surface Biotinylation - Advantage/Disadvantage (reply: 7)
  392. changed attchment of RMA/S cells after transfection, why? - (reply: 2)
  393. cell culture contamination - after transfection (reply: 15)
  394. How to prepare my own competent cells? - as above (reply: 5)
  395. removing rbc cells for cell purification - (reply: 5)
  396. cell lysis and DNA - sticky (reply: 3)
  397. 293T cells - (reply: 3)
  398. Frustrated with FACS (ONLY DEAD CELLS) - (reply: 8)
  399. few cells in the cell culture - (reply: 3)
  400. counting viable cells directly from wells - (reply: 7)
  401. how do i do flow cytometry for adherent cells? - (reply: 5)
  402. Problem with Monoclonal antibody production - why cells die after adding HAT?????? (reply: 6)
  403. pBABE-puro in 293T cells (not PhoenixA) - (reply: 5)
  404. stable transfections using SCC 9 cells - (reply: 5)
  405. Stable transfection of human adrenocortical cancer H295R cell line - (reply: 1)
  406. any tips for detecting phospho-ptotein in fixed adherent cells by flow cytometry - (reply: 2)
  407. 293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
  408. How to seed adherent cells evenly in 150mm dishes? - (reply: 12)
  409. PMA induced apoptosis in HEK cells - (reply: 4)
  410. Why different mammalian cell lysate preparation methods? - (reply: 6)
  411. cell lysis to investigate phosphorylated protein - (reply: 1)
  412. centrifugation speed for mouse neural stem cells - centrifugation speed for mouse neural stem cells (reply: 3)
  413. cy5 cell tracker dye - (reply: 2)
  414. transformation competent cells problem - fastbac vectors (reply: 2)
  415. Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
  416. transformation of competent cells - (reply: 2)
  417. Test for drug dose response in suspension cells before transfection - With Suspension cells, how do people distinguish difference between de (reply: 1)
  418. adipose stains? - specific stain to positively ID adipose cells (reply: 2)
  419. Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
  420. isolation of total RNA from bacteria attached to HeLa cells - (reply: 1)
  421. SURE E. coli strain - How make competents cells? (reply: 4)
  422. APC isolation from the Liver - How you can isolate the APC cells from the mice's liver (reply: 2)
  423. Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
  424. differentiated PC12 cells detatching - (reply: 3)
  425. Thp-1 cell transduction - (reply: 4)
  426. HIV-2 and MTT assay, cells lines to use - (reply: 2)
  427. tissue preparation for human/mouse islet cells - (reply: 1)
  428. Protein Extraction from cell media for western blot - (reply: 3)
  429. cell adhesion to plastic - (reply: 3)
  430. Epithelial cell-cell fusion - Protocol? (reply: 1)
  431. Chemically competent cells? - (reply: 3)
  432. cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
  433. bath sonicator for cell lysis - (reply: 2)
  434. serum starvation - induce cilia by serum starvation in Rpe-1 cells (reply: 7)
  435. Keep growing cells after an MTS/PMS assay - (reply: 1)
  436. Cells in log phase - (reply: 1)
  437. protocol for transfection(eletroporation) for HEK293 cell - (reply: 1)
  438. Stable cell line, polyclonal and monoclonal. - Which is better? (reply: 4)
  439. lentiviral transduction of suspension cell - (reply: 1)
  440. cell counting dilemma - (reply: 1)
  441. lentiviral transduction of suspension cell - (reply: 1)
  442. Immunostaining on bacterial cells? - (reply: 1)
  443. any kit or reagent for extracting both DNA and RNA from the same cell culture - (reply: 3)
  444. FACS sorting affects cell function? - FACS sorting affects cell function (reply: 2)
  445. cell counting calculation.... - (reply: 3)
  446. Phoenix 293T packaging cells - (reply: 3)
  447. suparnatant, cell free extract, crude extract - (reply: 4)
  448. competent cells transform 12 kb plasmid,urgent - (reply: 8)
  449. Whole cell extract on SDS/PAGE - (reply: 9)
  450. Making competent cells from current commercial stocks - (reply: 3)
  451. Calculation cell in 96 well plate - (reply: 1)
  452. G418 and resistant cells - can't kill the cells (reply: 8)
  453. MIN6 cells - (reply: 9)
  454. cell signaling & choice of the cell - (reply: 3)
  455. hard to transfect cell lines - (reply: 1)
  456. How can I separate nuclei from cytoplasm in trophoblastic cells - (reply: 1)
  457. salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
  458. Primary culture - Transparent cell? (reply: 2)
  459. transfection of chick cell - (reply: 3)
  460. non-adhernt cell washing - (reply: 1)
  461. Immunology on fixed bacteria - What are the basics of fixing cells for immunological analysis? (reply: 1)
  462. transfection of PC12 cell line - (reply: 1)
  463. strong attaching epithelial cells - molecular explanations? (reply: 1)
  464. How to save my primary cell lines! - (reply: 1)
  465. My cells die on poly-d-lysine coated plates! - (reply: 2)
  466. isolation of celmembrane of tissue cells - (reply: 2)
  467. How to grow frozen DB 3.1 cells - (reply: 1)
  468. REH cells and knockdowns - (reply: 1)
  469. Cell centrifugation at 3200xg - Can cells survive such speed? (reply: 4)
  470. transfection with PA-1 cells - (reply: 1)
  471. How to separate the antigen presenting cell from spleen of rat - (reply: 1)
  472. Transfection of T47D cells with FuGene in 10cm plates - (reply: 2)
  473. Immunofluorescence staining of spheroid cells - (reply: 3)
  474. How small cells can Flow cytometry detect? - Flow cytometry to detect a small cell (reply: 2)
  475. tg1 and hb2151 cells - (reply: 2)
  476. How to avoid central cell seeding in 6-well plates - (reply: 19)
  477. Same cell line but different growth rate - (reply: 4)
  478. Fixing Cells for Flowcytometry - Glutaraldehyde, Formalin, Microwave (reply: 11)
  479. estimating number of cells to seed - (reply: 2)
  480. cell dead after changing the medium to PBS buffer - (reply: 3)
  481. PC12 cell culture. Do I have to coat plates first? - (reply: 2)
  482. Can scintillation fluid be used to harvest adherent cells? - (reply: 1)
  483. Difference between cell invasion and cell migration - (reply: 4)
  484. too many dead cells why? - (reply: 3)
  485. Do cells transfected with lentivirus express specific unique surface markers - (reply: 2)
  486. Help: Cell Lysing - (reply: 5)
  487. Avoiding cell clumps - (reply: 7)
  488. can cells just stop having the gene? - (reply: 4)
  489. how important of Serum proportion of culture medium in cell culture - SOS (reply: 8)
  490. question about dendritic cells and antigen-specific T cells - (reply: 1)
  491. CD3 and CD28 T cell stimulation in TUBES - Technical question (reply: 4)
  492. cell wall protein extraction of gram-positive - (reply: 1)
  493. How to do the lysis of cells without white stuff? - (reply: 1)
  494. cell culture - a beginner - (reply: 3)
  495. any video or animation of experiments about cell and molecular biology - (reply: 1)
  496. how to calculate cell number? - (reply: 1)
  497. Flow Cytometry 2 Parameter Statistics, please help - Can you find standard deviation of % Positive of Total cells based on (reply: 1)
  498. cell concentration calculation - (reply: 2)
  499. isolating of mouse spleen Dendritic cells by nycodenz - (reply: 4)
  500. Suspension cell culture. - (reply: 5)
  501. PC12-Neurite out growth - less no of cells with neurite (reply: 4)
  502. p53 independent cell death - (reply: 2)
  503. Dendritic cell line media - Dendritic cell line media (reply: 1)
  504. Assay for cell purity determination - (reply: 2)
  505. fungal contamination - fungal in cell culture (reply: 4)
  506. Double-Thymidine block of Hela cells - (reply: 3)
  507. endothelial cell isolation from mouse - using magnetic dynabeads from DYNAL (Invitrogen) (reply: 6)
  508. OD600 and cell density correlation? - (reply: 1)
  509. T cell activation via CD3 and/or Cd28 need to solve a problem - T cell activation cd3 (reply: 5)
  510. Plant cell culture- How do prevent differentiation of callus cells - (reply: 2)
  511. Confocal microscopy with primary cell cultures - (reply: 8)
  512. stable cell line characterization and validation - (reply: 1)
  513. Trouble concentrating cell extract -please help! - (reply: 9)
  514. cell synchronization - (reply: 1)
  515. Proteinase k treatment of cells - (reply: 2)
  516. Cell lysis and osmotic shock - (reply: 7)
  517. De novo plasmid methylation in eukaryotic cells - (reply: 3)
  518. Collection of selective antibiotic conc for ALL cell lines - Please add yours! (reply: 6)
  519. endosome markers for mouse T cells - (reply: 3)
  520. what is the mechanism of transformation of a plasmid into a bacterial cell? - (reply: 5)
  521. ELISA Question - Finding the concentration of Ab from cell culture (reply: 4)
  522. Lyse cells with TCA - (reply: 1)
  523. separating normal epithelial cells from malignant transformed - protocol suggestions? (reply: 2)
  524. a question about screening transfected cells by GFP protein - (reply: 5)
  525. which -20 or -80 is more less degradation of cell lysates - (reply: 4)
  526. Washing during cell staining - FACS (reply: 2)
  527. How many days can fiexed cells be left before running flow - (reply: 1)
  528. Trypsinizing MCF10A cells - (reply: 1)
  529. transfection in NIH3T3 cells - (reply: 10)
  530. double digested PCR product into competent cell - (reply: 2)
  531. CAn trypsin be used for cell harvest? - (reply: 4)
  532. How to make an immortalized cell line/ How to determine IC50? - Protocol for developing a drug resistant cell line? (reply: 6)
  533. When are the cells virus free? - (reply: 6)
  534. cell culture contamination - (reply: 2)
  535. Remove BSA from cell culture medium - (reply: 5)
  536. Transfection of Breast Cancer Cell Lines - (reply: 2)
  537. Help! neural stem cells culture - no neurosphere after P1 - Please troubleshoot my protocol (reply: 2)
  538. Apply tranfection reagents DIRECTLY onto the cells. - After removing the medium. (reply: 4)
  539. aza treatment of D283 cells - aza treatment of D283 cells (reply: 1)
  540. Inconsistency of the cells~~ - (reply: 4)
  541. Quantifying HeLa cells in a 96 well plate - (reply: 2)
  542. Epithelium cell purification - (reply: 2)
  543. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
  544. Stable cell line stops growing :( - (reply: 6)
  545. siRNA protocol in floating cells - (reply: 1)
  546. Extraction of genomic DNA from 10 fold serial dilution of cells. - (reply: 5)
  547. Basics of Cell Culture - (reply: 2)
  548. if you plate 40 ul of your electrocomp cells only... - would you get any colonies? (reply: 13)
  549. Explant culture - All method of cell isolation coming from animal tissue (reply: 3)
  550. E.Coli. XA90 cell - transformation in XA90 cell (reply: 3)
  551. cells - (reply: 2)
  552. protocol for purificaiton of human mononucleus cells - (reply: 2)
  553. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
  554. cell membrane - (reply: 6)
  555. Endothelial cells purification - (reply: 2)
  556. How to make mES cells attch to differentiate? - (reply: 1)
  557. linear or supercoiled plasmid for creating stable cell line - (reply: 1)
  558. why some microorganisms form filament instead of single cell? - (reply: 1)
  559. can arginine be used as nitric oxide donor compound in cell culture. - kinly help!!!!!!!! (reply: 3)
  560. freezing HEK293 cells - (reply: 3)
  561. non-transfected cells - (reply: 3)
  562. rat1 cells freezing and thawing - how to culture rat1 fibroblasts (reply: 1)
  563. diluted cells too much? help - (reply: 1)
  564. primary cells better than 3T3 cells? - (reply: 3)
  565. Live cell transportation - (reply: 14)
  566. PBS composition for HeLa cells - (reply: 2)
  567. Flow Cytometry Intracellular and dead cell staining - (reply: 2)
  568. single channel and whole cell - (reply: 2)
  569. MTT assay of MCF-7 culture - what is duplication time of MCF-7 (reply: 3)
  570. Different sizes of PCR products from different competent cells - (reply: 4)
  571. Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (reply: 4)
  572. cell culture - (reply: 4)
  573. AzaC treatment of cells? - (reply: 2)
  574. Characterization of a smooth muscle cell line - (reply: 2)
  575. cell respiration - (reply: 1)
  576. How to isolate intact mitochondria from live cells? - (reply: 1)
  577. Curiosity: competent cell and glycerol - (reply: 7)
  578. DNA extraction- bacterial cells - kits - PLease help!!! (reply: 2)
  579. Is my RNA dead? - freezing cells after adding RNA lysis buffer (reply: 2)
  580. mES cells cultured on uncoated dishes/flasks grow well! - (reply: 1)
  581. cryopreservation agent missing in frozen cell stock - (reply: 3)
  582. transfecting primary cells - (reply: 1)
  583. Cell Culture Book - any suggestion? (reply: 29)
  584. 293F cells revival - (reply: 2)
  585. Does TPA induce cell proliferation/growth? - HT1080 cells (reply: 3)
  586. protocol on M cell model - (reply: 3)
  587. expression problems in mammalian cells - (reply: 2)
  588. transient transfection of a stable cell line? - (reply: 4)
  589. I want to inqure some questions about cell lines and cell phenotype - which cell line has the highest transfection efficiency as you know (reply: 1)
  590. WB with cell culture medium - (reply: 2)
  591. plasmid purification from mammalian cells? - (reply: 4)
  592. help- G0 synchrony of MCF10 A breast cells and MCF-7 cell lines - (reply: 1)
  593. Get rid of cell debris after transformation? - (reply: 1)
  594. monocytic cell line differentiation - (reply: 2)
  595. Obtaining cells from human tissue samples - (reply: 2)
  596. Changes on cells. - (reply: 5)
  597. 3T3-L1 cell differentiation - (reply: 2)
  598. stable cell line questions( cell type matter) - (reply: 2)
  599. Cell Culture with Human Plasma - (reply: 2)
  600. cell saparation from lymph nodes and spleen - how can I improve my yield? (reply: 1)
  601. G418 conc for HEK293 cells - (reply: 11)
  602. Protein loading control for RAW cell SUPERNATANTS in western blot - western blot (reply: 7)
  603. Transient transfection on a stable cell line - (reply: 2)
  604. Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
  605. Infection efficiency of non-adherent cells - (reply: 3)
  606. Are there TGFB receptors in 293T cells? - ..and if you don't know, how would you find out? (reply: 2)
  607. Which medium do you use to grow HEK293 cells? - (reply: 7)
  608. Advantages to eukaryotic cells of having a nucleus? - (reply: 9)
  609. Contamination of unique cell line (eeek!) - (reply: 5)
  610. how to seperate cells from the dead and the live - (reply: 9)
  611. Expression in other mammalian cells with human H1 promoter - (reply: 1)
  612. Which cell lines are best? - (reply: 6)
  613. Knockout cell lines? - (reply: 4)
  614. If I accidentally injecting human breast cancer cell lines into my finger - What happens? (reply: 7)
  615. competen cell - (reply: 3)
  616. Tumour cells grow slower than normal cells, why? - cell proliferation rate in normal vs tumour cell (reply: 2)
  617. paraformaldehyde solution - for cell fixation (reply: 19)
  618. How to seed cells evenly in small well plates - (reply: 27)
  619. remedy against cell contamination - (reply: 4)
  620. stain similar to neutral red - stain unfixed cells directly (reply: 4)
  621. How to lift attached CD14 cells? - (reply: 3)
  622. How can i decontaminated my cell culture ? - (reply: 4)
  623. Cell fixation problem - (reply: 3)
  624. Hela cells and stable transfection help please! - (reply: 6)
  625. Is cell culture considered in vivo? - (reply: 4)
  626. Transfecting KG 1 cell - (reply: 3)
  627. cell stain, nonfluroescent - (reply: 4)
  628. Need to Slow down Cell Culture growth - (reply: 2)
  629. What size needles I should use for cell extraction protein - (reply: 1)
  630. Cell adhesion assay - (reply: 2)
  631. Tetracycline in cell culture media - (reply: 5)
  632. Treatment of cancer cell - (reply: 2)
  633. why centrifuge before doing a cell count? - (reply: 1)
  634. amount of proteins isolated from cells - (reply: 2)
  635. clumping cells - s. aureus wildtyp cells (reply: 3)
  636. Primary Hippocampal cell culture-DEAD! - (reply: 3)
  637. How long can cells survive at 4 degree - (reply: 2)
  638. Medium for adherent cells and serum/protein free - (reply: 2)
  639. Mouse ES cell won't attach - (reply: 1)
  640. monitor cell growth - (reply: 1)
  641. RNA extraction from formaldehide fixed cells - (reply: 2)
  642. vector for expression of tag on cell surface - (reply: 7)
  643. Fibroblats contamination in primary cell cultures - Fibroblats contamination in primary cell cultures (reply: 6)
  644. why competent cells take up only 1 type of plasmids? - (reply: 7)
  645. cell lysis buffer recipe - simple question (reply: 6)
  646. Help with HeLa cells culture... - (reply: 10)
  647. FACSing of MCF-7 cells - (reply: 3)
  648. FLAG-Tag Protein expression in MCF-7 - (reply: 8)
  649. Why tumor cells are hard to culture - (reply: 3)
  650. Electroporation of stem cells with DNA in Top10 - endotoxins? - (reply: 4)
  651. magnetic affect of ameoba cells? - how can i make this a science fair project (reply: 1)
  652. Evaluation of Boyden Chamber Assay - how to count cells? (reply: 4)
  653. Cell Fixation - Ethanol or Methanol? - (reply: 4)
  654. C6 Cells Contaminated - What do you think it is? (reply: 6)
  655. hippocampal cell culture - I need a help (reply: 5)
  656. How to make electrocompetent cells without refrigerated centrifugue - (reply: 3)
  657. Bacterial cell adhesion assay - (reply: 6)
  658. T cell culture - ag specific T cell culture (reply: 1)
  659. ChIP from low amount of cells - (reply: 1)
  660. What's difference between RPMI and DMEM - which media I should use to culture cells after transfection? (reply: 3)
  661. MCF-7 staining protocol - nile red, or oil red o? (reply: 2)
  662. Long-time storage of tissues/cells with fluorescent tracers - (reply: 4)
  663. Transfection of 293 cells for protein expression - optimum conditions for transfection!!! (reply: 5)
  664. Why is non-viral transfection efficiency of endothelial cells low? - (reply: 1)
  665. Hepg2 cells resistant to trypsin digestion - (reply: 7)
  666. Immunofluorescence of frozen cell culture inserts - (reply: 6)
  667. T cell proliferation assay - (reply: 1)
  668. SHSY5Y cell transfection - (reply: 5)
  669. what is the function of the morpholinopropanesulfonic acid? - cell labeling (reply: 4)
  670. mold problems in cell culture - (reply: 1)
  671. Progenitor cell type - (reply: 1)
  672. can the phoenix cell be transfected using lipofectamine ? - (reply: 2)
  673. Increase in cell viability? - (reply: 1)
  674. Making frozen cell pellets competent - (reply: 3)
  675. Unhealthy Passaging? - passaging cells too much... will they go bad? (reply: 4)
  676. Dead cell removal -why? - (reply: 3)
  677. cancer cell transfections - (reply: 2)
  678. About the cell lysis methods - (reply: 8)
  679. Need prototocol for membrane fractionation from mammalian cells - (reply: 1)
  680. Culturing of HEP G2 cells - (reply: 5)
  681. low viability of stably transfected cell line from cryostock - (reply: 1)
  682. Can I use 3H-Thymidine on adhesive cell for cell proliferation assay - (reply: 8)
  683. How 2 Calculate cells/mL to cells needed per well? - EZ way to determine how much cell suspension to add/well for a certain number of (reply: 6)
  684. lipid content HepG2 cells - (reply: 2)
  685. M-NFS60 cell line revival - Revival protocol (reply: 3)
  686. Any effect of different culture dishe size on cell? - (reply: 20)
  687. doubling rate of MDA-MB-453 cells - (reply: 1)
  688. Primary endothelial cells went differentiated - Medium, serum or other factors? (reply: 6)
  689. Trypan blue cell death assay on cardiac myocytes - (reply: 3)
  690. What is an acceptable CO2% range for growing cells in DMEM? - (reply: 8)
  691. mycoplasma effects on cells - will mycoplasma kill the cells? (reply: 2)
  692. method for staining of invisible cell pellets? - (reply: 1)
  693. rna isolation from bone marrow cells - (reply: 1)
  694. Monocyte-derived DC generation: CD14 separation or adherent cells better? - (reply: 1)
  695. GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
  696. transplantable rats or mice cell line in nude mice - (reply: 1)
  697. How to tell cell are contaminated by fungi - (reply: 4)
  698. Need protocol: CFSE Staining/ primary cells - (reply: 2)
  699. why do you prepare heat-stable fractions of cells? - (reply: 1)
  700. Puromycin and HEK293 cells - (reply: 3)
  701. neuronal markers for the mature level for spinal cord cell - (reply: 4)
  702. tissue n cell culture - (reply: 3)
  703. do cells benefit from being of different age? - (reply: 2)
  704. cell culture contamination - (reply: 2)
  705. Can I call my cell a established cell line? - (reply: 1)
  706. how long can cell lines be carried? - (reply: 5)
  707. transfection of HeLa cells - (reply: 4)
  708. cell stain and the fixation? - (reply: 2)
  709. Cell Differentiation - Time periods and environment for proper cell differentiation (reply: 1)
  710. Cell Lysis - (reply: 2)
  711. Does anybody have MIN6 cells? - MIN6 Cells (reply: 1)
  712. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  713. questions about MCF-7 cells - (reply: 10)
  714. detachment of my cells?/ - (reply: 7)
  715. depolymerizing microtubules in 293 cells - (reply: 1)
  716. cell proliferation assays - (reply: 2)
  717. Retroviral transduction of cells - troubleshooting help please! - (reply: 8)
  718. After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
  719. should dead cell detach? - (reply: 2)
  720. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
  721. Stem Leydig cells - (reply: 1)
  722. adenovirus vs. retrovirus cell infection--pros and cons? - infecting PC12 cells and overall general toxicity (reply: 3)
  723. problems with Clonetech 10G cells - (reply: 1)
  724. THP-1 cell line - Passage number affect phagocytosis (reply: 12)
  725. MTT test for the suspension culture - Raji cell and K562 (reply: 4)
  726. maximum plasmids can be co-transfected into Hela cells - (reply: 4)
  727. T cell proliferation assay - (reply: 3)
  728. Culture of adherent SH-Sy5y Neuroblastoma Cell Line - (reply: 5)
  729. CCR5 cytometry on dendritic cells, probles with isotype control - (reply: 2)
  730. transformation of competent cells with recombinant plasmids - (reply: 2)
  731. plasmids smaller than empty vector - using expensive cells - still not working (reply: 1)
  732. Knocking down the cPLA2,sPLA2 in Cancer cells - (reply: 3)
  733. Primary cell cutures - what is this (reply: 2)
  734. chemical that impede cell growth - (reply: 1)
  735. EM, cell preparation: to coat or not to coat slide with collagen - electron microscopy (reply: 1)
  736. microglia cell culture - (reply: 5)
  737. siRNA delivery in non-dividing cells - (reply: 8)
  738. PC12 cell culture - (reply: 5)
  739. Turbo Competent Cells - (reply: 3)
  740. yeast contamination in my cell culture - (reply: 11)
  741. Cell Biologists working in 3d cell cultures - (reply: 1)
  742. How to identify whether a cell line might posses stem cell characters - Any general molecular markers for stem cells? (reply: 4)
  743. Use of antibiotics in cell culture? - (reply: 5)
  744. how to prepare total cell lysate for Western blot? - (reply: 10)
  745. doxorubicin treated cells - (reply: 1)
  746. Problem with cell line/G418 - (reply: 8)
  747. Chondrocytes - Need a Chondrocyte cell line (reply: 2)
  748. RNA isolation from trypsinized cells - (reply: 4)
  749. how to solve the problem cell culture contamination? - (reply: 2)
  750. how to know that ur cells are confluent? - (reply: 2)
  751. How to make a stable cell line? Help! - (reply: 3)
  752. time when cells go into apoptosis - (reply: 2)
  753. doxorubicin treated cell cutlures? - (reply: 1)
  754. Cell line passage number? - (reply: 3)
  755. basic techniques for cell treatment - (reply: 6)
  756. Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
  757. sodium azide on living cells? - (reply: 3)
  758. Whole cell extraction buffer... - does any one have the recipe? (reply: 1)
  759. Fetal bovine serum and cell culture - (reply: 5)
  760. optimization of cell isolation protocol - optimization of cell isolation protocol (reply: 1)
  761. Why starving cells before a tratment? - (reply: 6)
  762. cell death pathways, how many? - and their basic differences.... (reply: 6)
  763. Measuring cell viability via luminescence... plate type? - Promega CellTiter-Glo kit (reply: 2)
  764. Functional study with PC12 cells - (reply: 16)
  765. lysis of cells - how to lyse cells (reply: 6)
  766. Cell line to be used for protein expression an purification - (reply: 8)
  767. TLR expression on Vero cells - (reply: 2)
  768. Role of DMSO in Cell fusion. - (reply: 2)
  769. Need protocol for mouse ES cell stable transfection - (reply: 1)
  770. does phenol:chloroform extracted plasmid creats problem in Mammalian cell cultur - (reply: 5)
  771. in vitro cell proliferation assay - use of the DMSO as drug solvents (very urgent) (reply: 3)
  772. Are transiently transfected DNA methylated in mammalian cells? - (reply: 3)
  773. why transfected cells has less RNA? - (reply: 2)
  774. methods of inducing stress in cell culture - (reply: 8)
  775. Transfectiion of H295R cells by Fugene6 - (reply: 8)
  776. Mammalian Cell adhesion and proliferation on biomaterials - (reply: 1)
  777. removal carbohydrates cell surfaces - how to "shave off" sugars from cell surfaces (reply: 1)
  778. siRNA transfection of MIN6 cells - (reply: 2)
  779. Is it possible to harvest E.coli cells for purification of DNA from LB agar and - (reply: 5)
  780. Cells survived after G418 selection are transfected cells? - (reply: 2)
  781. Saos-2 cells transfection - (reply: 1)
  782. how to make neutrophil cell lysates for Western blot? - (reply: 3)
  783. getting rid of shaky hands during cell culture/pipetting - (reply: 9)
  784. Mouse endothelial cell line - (reply: 1)
  785. MCF-7 growing in suspension - (reply: 4)
  786. infected cell line? - fungal contamination? (reply: 5)
  787. Growing cell lines from frozen tissue samples - Is this even possible??? (reply: 8)
  788. Cell Growth - Help! (reply: 7)
  789. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
  790. Trypsin:EDTA cell detachment process - (reply: 6)
  791. M17 cell transfection - (reply: 1)
  792. Stem cell tranfsfection - Electroporation or ca-phos precipitated DNA? (reply: 7)
  793. Cell Viability VS Cell Proliferation assays - (reply: 2)
  794. crude cell lysate - storage of crude cell lysate (reply: 3)
  795. CHIP - Harvesting cells by trypsin - (reply: 1)
  796. Cell passage nomenclature - (reply: 4)
  797. Can DH5α be the host cell of the PCR-XL-TOPO from Invitrogen? - Help me (reply: 4)
  798. Techniques for counting apoptotic cells - (reply: 6)
  799. Persistent cell culture contamination by fungi and bacteria - Cell culture, cell biology (reply: 6)
  800. Adult stem cell differentiation - (reply: 2)
  801. Cell Lysates for western - (reply: 4)
  802. transfecting DNA into Jurkat cell - (reply: 1)
  803. single cells from human tissue? - need protocols (reply: 1)
  804. Tryphan Blue staining for cell viability - (reply: 1)
  805. Anyone has experience to grow sespension 293 cells? - (reply: 1)
  806. Cornea Epithelial Cell culture - (reply: 1)
  807. what kind of adherent cell lines can be analyzed by FCM through Annexin V/PI sta - (reply: 2)
  808. cell cycle data normalization - (reply: 1)
  809. Why are lysosomes considered the cell's "cleanup crew"? - i need help (reply: 1)
  810. Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line (reply: 3)
  811. Can I extract nuclear proteins in transfected cells? - (reply: 2)
  812. Ordering CHO-K1 cells - (reply: 1)
  813. why my IFN gamma treated cells still high in TER? - (reply: 1)
  814. co-transfection in NRK cells - (reply: 1)
  815. 293T cell line, how to prevent cell clusters? - (reply: 1)
  816. Cell cycle analysis - Include floating cells or not?? (reply: 7)
  817. Death of Cells After Defrosting - MDBK cells not surviving (reply: 2)
  818. Extensive cell death after transfection in a 24-well plate - (reply: 3)
  819. Hela cells clumping in center of well of 96well plate. - (reply: 1)
  820. mouse B cells - (reply: 1)
  821. reasons for apoptosis in primary cell culture? - (reply: 2)
  822. Problems with cell lysate - (reply: 4)
  823. competent cells - making compentent cells (reply: 2)
  824. Establishment of human embryonic stem cell - (reply: 3)
  825. Help: got the stable cell line without my target gene - (reply: 3)
  826. Sticky cells - (reply: 3)
  827. ask for 721.221 cell line - (reply: 3)
  828. Spectrophotomery of cell 550 vs. 600 - spectrophotometry for cells (reply: 2)
  829. extract peptides from tissue/cell culture for ELISA kits - (reply: 3)
  830. looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
  831. endothelial cells transfection - (reply: 2)
  832. How many passages cells lines can survive before senescence? - (reply: 8)
  833. RNA extraction from small number of cells - (reply: 3)
  834. NMDA tox and Rat Cerebellar Granular Cells ! - NMDA tox assay for Rat CGC's ???? (reply: 3)
  835. Host cells for pBELOBAC11 - (reply: 4)
  836. URGENT-Selecting for transformed bacteria cells - (reply: 1)
  837. mammalian cells invaded by intracellular microbes - how can I decontaminate? (reply: 3)
  838. RAW 264.7 cell line transfection - (reply: 1)
  839. change adherent cells into suspenion cells - (reply: 1)
  840. detection of GFP fluorescence in whole cell bacteria - (reply: 1)
  841. How to fix and stain lymphoblastoid cell onto glass slides - (reply: 5)
  842. no growth on plate agar for competent cell - (reply: 6)
  843. starvation medium recipe for Hela cells - (reply: 2)
  844. Inturpreting cryopreservation data - I need help applying data I'm colleting with the freezing of E. coli cells t (reply: 1)
  845. transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
  846. siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
  847. problem transfection Hela cells - (reply: 8)
  848. MCF-7 Cell line - (reply: 6)
  849. Switching CHO cells media from high to low glucose - how would that effect cells? (reply: 1)
  850. live cell confocal microscopy - protocol please? (reply: 2)
  851. Will low concentration of ethanol affect cell line? - (reply: 1)
  852. Isolation and culture of white blood cells. - (reply: 1)
  853. Neuro2A cell culture Death and Debris - Problem keeping cells alive for more than a week. (reply: 2)
  854. Human Neural Stem Cells wanted - (reply: 1)
  855. plating cells for transfection - (reply: 2)
  856. cytotoxicity and cell proliferation assay - preference of dyes for cell viability (reply: 2)
  857. protocol for electrocompetent cells preparation - (reply: 5)
  858. Transient transfection on a stable cell line - (reply: 6)
  859. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
  860. need help for culture protacal of new stem cell - (reply: 5)
  861. Activating human T cells - (reply: 8)
  862. Storage of whole cell lysates - How? (reply: 2)
  863. Why use PBS before trypsinizing cells - (reply: 4)
  864. Question about doing RNAi in primary cells - (reply: 3)
  865. A Procedure for Freezing and Thawing Bacterial Cells - (reply: 2)
  866. Detecting proteins in Mouse embryonic fibroblast - Any tips on making cell lysates of MEFs? (reply: 2)
  867. Cell Tracing Software - Searching for a quicker way!!! (reply: 2)
  868. cells going away - (reply: 3)
  869. pituitary cell lines sex - (reply: 1)
  870. Raji cell subculture - Culturing Raji cells: DMEM or RPMI? (reply: 4)
  871. How to tell if competent cells or SOC are contaminated? - (reply: 14)
  872. Transcient transfection of HaCaT cells - which is the most effective method? - (reply: 2)
  873. Dissociation of HaCaT cells without using trypsin - will PBS/EDTA work? - (reply: 4)
  874. Where to get Granta 536 Cells - (reply: 4)
  875. Moving Dots in cell culture - Contamination? - (reply: 7)
  876. Drosophila cell culture - (reply: 3)
  877. Culture problems of CRL-2331 cells (ATCC) - (reply: 3)
  878. electroporation of PC12 cells - (reply: 1)
  879. RNA prep (Trizol) with small cell numbers - How low can you go? (reply: 13)
  880. Slow growing cells - (reply: 6)
  881. About cell diameter - Help,please browse (reply: 1)
  882. Protoreaction of Protoplasm - New approaches to Cell Biology and Physiology (reply: 1)
  883. Problems with Ba/F3 cell culture - (reply: 1)
  884. Problem growing dh5alpha cell - (reply: 2)
  885. Cells ignore my cover glass, growing around it on the plastic - What cower glass should be? (reply: 1)
  886. DH5 alpha competent cells instead of M15? - competent cells for pQE30 construct (reply: 2)
  887. Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy? (reply: 2)
  888. Transfection of coronary artery endothelial cells - (reply: 1)
  889. media for revival of cell lines - (reply: 1)
  890. transfection in eukaryotic cells - (reply: 1)
  891. stable transfection of MDAMB231 and Malme3M cells. - (reply: 1)
  892. DNA fragmentation in apoptotic cell - (reply: 1)
  893. Inactivated/activated FBS - is it critical for healthy cells (reply: 7)
  894. Adding H2O2 to cell culture - How to calculate? (reply: 4)
  895. cell line for lentivirus titration - (reply: 1)
  896. ask some question about cell size of brain - help (reply: 2)
  897. Role of PBS in cell culturing - (reply: 1)
  898. How to culture rat dorsal root ganglia cell? - (reply: 1)
  899. Protocal for DAPI/ Hoechst staining adhering cells - (reply: 3)
  900. how 2 quantify bacteria cells? - (reply: 4)
  901. Shipping cells in suspension culture - (reply: 6)
  902. hela cells - (reply: 12)
  903. cre recombinase - in cell lines (reply: 2)
  904. siRNA transfection reagent for primary cultured cells - (reply: 2)
  905. pcDNA 3.1/pTarget and stable cell line - (reply: 10)
  906. Cells are stuck - (reply: 4)
  907. Serum for cell culture - difference on regular vs dialyzed vs heat-inactivated FBS (reply: 3)
  908. Red Blood Cells exploding ! - (reply: 2)
  909. How does the cells of the innate immunity gets rid of the pathogens? - (reply: 4)
  910. Baculovirus Expression - Sonicate infected cells? (reply: 2)
  911. Synchronize hela cells with double thymidine block - mechanism of double thymidine block (reply: 1)
  912. house-keeping primers for Cos7 cells - (reply: 4)
  913. RAW264.7 Cells .. anyone familiar with this cell line? - need help please (reply: 2)
  914. adapting cells to CO2-Independent Medium - (reply: 1)
  915. DNA methylation in Raji cell line - (reply: 1)
  916. How long does take the cell cycle in HepG2 cell line? - (reply: 1)
  917. T cell activation. - (reply: 1)
  918. genetic markers for cell lines - (reply: 1)
  919. Needing a consistant cell suspension - Non-homogenous cells make me crazy! (reply: 2)
  920. transfecting HeLa cells for the first time... - help needed....please (reply: 4)
  921. bisulfite sequencing: cell lines vs tumors - (reply: 3)
  922. cell type specificity ? - (reply: 1)
  923. Passage number and behaviour of cell line - (reply: 10)
  924. what's the difference between 293FT and 293T - stable cell lines from 293Ft or 293T cell lines (reply: 1)
  925. Serum starvation of cells - (reply: 2)
  926. Transfection - Hepg2 cell lines (reply: 2)
  927. change in cell morphology in culture - cell culture (reply: 2)
  928. Has anyone tried to sonicate Jurkat cells for ChIP assay? - (reply: 7)
  929. macrophage cell line - (reply: 3)
  930. Suspension Culture - 293H cells clumping (reply: 2)
  931. My competent cells are misguiding me - (reply: 3)
  932. Did anyone ever do DCF assay using M17 cell line? - For help (reply: 1)
  933. Cell Membrane Permeability - (reply: 1)
  934. DRG cell culture - (reply: 6)
  935. nuclear extract - protocol for cell fractioning (reply: 1)
  936. Question about stem cell attachment - (reply: 1)
  937. maximum passage number for T47D cells - (reply: 2)
  938. transfection of Hek 293A cells - (reply: 5)
  939. cell scraper - (reply: 4)
  940. Transfection of cells by viral DNA - (reply: 4)
  941. neural progenitor cell death in multi-well plate - (reply: 10)
  942. Hybridoma Cell lines - How to do it (reply: 1)
  943. more contamination owes - spherical cell like contamination (reply: 8)
  944. C2C12 cells model for differentiation - (reply: 1)
  945. Resuspending mycobacterial cells? - (reply: 2)
  946. Glycerol gradients - glycerol gradients for cell extract fractionation (reply: 2)
  947. Fibroblast and epithelial cells in tumor samples and primary cultures - (reply: 2)
  948. DNA Repair in different cells - (reply: 2)
  949. Getting crazy because of cell contamination - (reply: 27)
  950. lncap cell culture - (reply: 3)
  951. cell fractionation plus IP - (reply: 12)
  952. Lab dealing with factors affecting cell membrane permeability - (reply: 1)
  953. passaging 293T cells - (reply: 5)
  954. lysosomes in cells - (reply: 2)
  955. Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
  956. making more chemically competent cells - (reply: 6)
  957. Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
  958. anyone has EcR293 cells? - (reply: 5)
  959. Searching specific Cell line - (reply: 1)
  960. Help me....About the 3T3-L1 Cells - (reply: 2)
  961. Mouse ES cell culture - ES cell culture for the purpose of gene targeting (reply: 3)
  962. HeLa in suspension culture - I want to keep HeLa cells in suspension culture (reply: 2)
  963. How many cells (nuclei) per ml pellet? - (reply: 3)
  964. Glycyrrhizin (GL) and cell culture - (reply: 1)
  965. transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
  966. ES and MEF cell - (reply: 1)
  967. How to reduce cell clumping for immunocytostaining - (reply: 1)
  968. primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
  969. ATCC cell lines - How is your experience? (reply: 1)
  970. siRNA transfection of non-adherent lymphoma cells - (reply: 2)
  971. need help on learning cell and tissue culturing - (reply: 6)
  972. can I re-freeze the 4% paraformaldehyde for longer storage? - leave cells in 4% paraformaldehyde 4 FACS? (reply: 3)
  973. Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
  974. Silly question: is DMSO sterile by itself, to add it to cells? - Lab stock bottle could be used? (reply: 6)
  975. Cell clumping during thawing - (reply: 2)
  976. mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS (reply: 1)
  977. what's the best system to get siRNA into a cell? suggestions - (reply: 1)
  978. Lysis buffer - muscle cells lysis (reply: 1)
  979. Help with defective cell lines - (reply: 1)
  980. How to measure neuronal cell differentiation? - (reply: 1)
  981. Cell Fusion - Fusing spleen cells with myeloma cells using PEG (reply: 1)
  982. Cell Death. optical spillovers.alternative assay - Need an assay for apoptosis (reply: 1)
  983. Fixing cells for performing immunoflourescense? - Help with protocol (reply: 3)
  984. Difference between cell proliferation and cytotoxicity kits? - (reply: 2)
  985. Bioruptor for sonication in mammalian cells - (reply: 2)
  986. Who killed my cells??? - Toxicity during transfection (reply: 3)
  987. Transfection reagent for HT-29 and HCT-116 cells - (reply: 4)
  988. MCF-10A transfection question - what to do with slow growing cells. . . (reply: 1)
  989. FACS - permeabilization of cells without killing (reply: 1)
  990. DC harvesting for FACS - dendritic cells (reply: 2)
  991. about cell sonication - (reply: 2)
  992. Subculturing cells grown in serum free media - (reply: 2)
  993. How to screen mammalian cells clones? - (reply: 3)
  994. Commonly used endothelial cells - (reply: 2)
  995. Primary Cell Culture - Tips for PHK culture (reply: 2)
  996. Primary endothelial cells not attaching - (reply: 5)
  997. HepG2 cell line - what is the doubling time for HepG2 cell line? (reply: 11)
  998. Has anybody generated stable cell lines using HepG2 cells? - Stable cell lines (reply: 5)
  999. calculating mean doubling times for adhesion cells - Mean Doubling time for adhesion cells (reply: 1)
  1000. Cell attachment - cells and jam (reply: 1)
  1001. Cell count on MCF-7 - (reply: 5)
  1002. 5-AZA/TSA upregulation of gene in cell lines - Gene induction (reply: 3)
  1003. A quick question about sonication to lyse cells - (reply: 4)
  1004. siRNA in Drosophila S2 cells? - (reply: 4)
  1005. extract RNA from frozen cells - (reply: 6)
  1006. Function of beta-mercaptoethanol in cell culture medium - (reply: 6)
  1007. Black Debris in T-Cells - debris in my cell culture (reply: 3)
  1008. cell cycle analysis of transiently transfected cells - (reply: 1)
  1009. Storage of cell lysate - (reply: 7)
  1010. cancer cells on agar - growing adherent cancer cells on agar plate (reply: 2)
  1011. Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
  1012. Need advice on FACS for cell cycle analysis - (reply: 3)
  1013. Extracellular Matrix - 3t3 cells lay down ecm? (reply: 2)
  1014. kato-III transfection - Help with transfecting semi-adherent Kato-III cell (reply: 1)
  1015. DNA Sonication of quiescent cells - (reply: 1)
  1016. dendritic cell isolation - cell culture (reply: 1)
  1017. Morphologic changes of HeLa cell after RNAi - (reply: 7)
  1018. MCF-7 cell line with fibroblast - (reply: 1)
  1019. Apoptosis and Annexin V - Protocol for cell detatchment ? (reply: 3)
  1020. cell immunofluorescence - (reply: 14)
  1021. How to buy MCF-7/ADR? - (reply: 2)
  1022. assays to assess cancer cell growth? - (reply: 4)
  1023. Transforming DH5 alpha cells with pUC19 vector - Cloning (reply: 3)
  1024. Insect cell sonication protocol? - Protein Purification (reply: 1)
  1025. Fixation of cell culture with Formaldehyde - need protocol (reply: 3)
  1026. best cell colture media for in vivo imaging - (reply: 3)
  1027. A question about cell stain: fix the cell have fusion protein - (reply: 1)
  1028. How to get rid of contamination in stable transfected cells - (reply: 3)
  1029. how can i culture virus in primary cell lines? - microbiology (reply: 1)
  1030. coatings for growing cells on glass coverslips - tissue culture and confocal microscopy (reply: 4)
  1031. can i freeze cell lysates for western blotting? - (reply: 3)
  1032. real-time RT-PCR one cell - (reply: 2)
  1033. Screening stably transfected cells? so little? - (reply: 4)
  1034. confocal microscopy, fluoro beads, 3 types of cells on one slide - (reply: 1)
  1035. Cell cycle question - CKIs - (reply: 2)
  1036. Tansfection in drosophila S2 cells with pEGFP-N1 - pEGFP-N1 vector @ drosophila S2 cells (reply: 1)
  1037. Definition of cell confluency - (reply: 6)
  1038. Troubles with labeling epithelial cells with CFDA-SE - Troubles with labeling epithelial cells with CFDA- (reply: 1)
  1039. hybridoma cell question - (reply: 4)
  1040. differentiate THP-1 cells - differentiate THP-1 cells (reply: 4)
  1041. How to sort real GFP positive cells? - autofluorescence VS GFP (reply: 2)
  1042. Do you reuse cell culture vessels? - (reply: 3)
  1043. more help: cell culture problem - (reply: 1)
  1044. cell cycle of NIH3T3 - cell cycle of NIH3T3 (reply: 1)
  1045. Osmolarity of the mammalian cell medium - (reply: 2)
  1046. Transferring cells from a 96 to 24 well plate - (reply: 1)
  1047. Cell counting - (reply: 3)
  1048. RCF safe for mammalian cell - (reply: 1)
  1049. Incubate suspension cells? - (reply: 1)
  1050. Sludge when growing insect cells - (reply: 1)
  1051. Trypan blue cell counting - (reply: 7)
  1052. S2 cell expression - Protein expression (reply: 2)
  1053. How do you differentiate Hep G2 cells? - Hep G2 cells (reply: 6)
  1054. How to make a stable cell line using suspension cells - (reply: 5)
  1055. Frozen transformed bacterial cells problems - (reply: 6)
  1056. does fungi swim or move? - cell culture contamination (reply: 8)
  1057. Cell culture contamination - small black adherent dots!! (reply: 4)
  1058. passage number in mammalian cells - (reply: 9)
  1059. freezing of cells - (reply: 9)
  1060. how to prevent HEK293 cells from coming off the plate - (reply: 4)
  1061. transfection problem---SOS! - why the cells should be split before transfection? (reply: 7)
  1062. Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
  1063. MDA-MB-231 cell culture - (reply: 7)
  1064. Preparation of competent cells of pichia - (reply: 1)
  1065. Problem in culturing PZ-HPV-7 cell line - (reply: 4)
  1066. Murine markers on hematopoietic stem cells (CD34, Sca-1, etc) - Looking to identify markers on megakaryocytic progenitors (reply: 2)
  1067. breast primary culture cells - (reply: 2)
  1068. filtering for cell culture stuff - filter cell culture (reply: 5)
  1069. transfection of single copy of plasmid per cell - (reply: 3)
  1070. looking for cell line: CEMX174(T1) - (reply: 2)
  1071. SF9 cells - (reply: 2)
  1072. Natural Killer cells - (reply: 1)
  1073. Stable Transfection in Sf9 cells - Stable Transfection in Sf9 cells (reply: 5)
  1074. B-gal staining of cells - (reply: 1)
  1075. Need HeLa cell extract prepartion protocol - wait your answer online (reply: 1)
  1076. bands from tissue one size, from cells - size doubles - help! (reply: 4)
  1077. Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
  1078. red blood cell lysis - (reply: 6)
  1079. RNAi in Jurkat cells - (reply: 3)
  1080. Transfection - Cell death after transfection - (reply: 6)
  1081. FACS analysers - Purchase of a FACS analyser and cell sorter (reply: 3)
  1082. Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)
  1083. co-culture of two adherent cells - (reply: 4)
  1084. Cell lysis when isolating dendritic cells - (reply: 1)
  1085. hep-2 & Hep-G2 cell line - difference between hep-2 & Hep-G2 cell line (reply: 22)
  1086. Cell contamination, needs the help urgently - How to remove? (reply: 2)
  1087. EDTA only for cell dissociation - (reply: 7)
  1088. where to buy sterile grease used for isolate cell clone? - (reply: 2)
  1089. please recommend me a RNA isolation kit - RNA isolation from 100 tumor cells (reply: 1)
  1090. Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
  1091. RT-PCR of different cell lines - (reply: 1)
  1092. cancer cell culture - protocols on cancer cell culture (reply: 8)
  1093. Mammalian cell line - monolayer - monolayer growth of mammalian cell line (reply: 1)
  1094. How many cell passages to be safe ? - (reply: 8)
  1095. Contamination issue in cell culture - (reply: 109)
  1096. Visualizing Cell Pellet - (reply: 1)
  1097. LCL B cell culture - Death within 24 hours of thawing - LCL b cells are dying within 24 hours of thawing. (reply: 3)
  1098. Stable transfection of S2 cells - (reply: 1)
  1099. ATDC5 cell line - i am looking for this cell line (reply: 2)
  1100. Dendritic cell -isolation ---viabillity is very low - Immunology (reply: 3)
  1101. estrogen and MCF-7 proliferation assay - (reply: 2)
  1102. cell fixation - How to fix cells? (reply: 10)
  1103. what types of cells is a mammalian cell? - (reply: 3)
  1104. Help with stable cell lines - (reply: 3)
  1105. cell titer glo - relative light unit (reply: 1)
  1106. non-adherent cells immunostaining - lymphocytes immunocytochemistry (reply: 3)
  1107. Equipment requirements to culture cells - information needed on kidney cells (reply: 2)
  1108. How little competent cell do you use for transformation - (reply: 2)
  1109. Freezing and thawing of cells - (reply: 112)
  1110. Human Macrophage cell line - quick help please - (reply: 3)
  1111. cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
  1112. Primary epithelial cell line - Isolation of epithelial cells (reply: 3)
  1113. Cell Biology - protocol for reactive oxgyen species (reply: 1)
  1114. Plant cell preparation - Flow cytometry (reply: 1)
  1115. poly(A)-RNA extraction from cells - (reply: 1)
  1116. Transcriptional activity in Stable cell line. - (reply: 3)
  1117. A question about adenovirus infection 293 cells - (reply: 5)
  1118. Methods for mechanical disruption of cell - (reply: 3)
  1119. Cell disruption without sonication - (reply: 4)
  1120. pBABE Comp. Cells - Special cells for transformation (reply: 3)
  1121. Any ideals for cell fragmentation? - (reply: 2)
  1122. cell lysis - How to lysis cells (reply: 2)
  1123. DMSO and cell cycle - (reply: 2)
  1124. cell culture RNA extraction - cell culture RNA extraction (reply: 4)
  1125. Origami B cells grow too slowly!! - (reply: 1)
  1126. about LNCap cell lines - (reply: 1)
  1127. CBP expression problems in mammalian cells - (reply: 1)
  1128. problem by using percoll to purify primary cell - (reply: 1)
  1129. the host cell of plasmid:pBmIEGLacZ-BmIEGhyg - (reply: 1)
  1130. T cell proliferation - problems (reply: 1)
  1131. lymphocytes' RNA extraction - How many cells (reply: 1)
  1132. MCF-7 - (reply: 1)
  1133. 4 layers instead of a monolayer in cell culture - (reply: 2)
  1134. Cell staining - (reply: 1)
  1135. How to keep the shape of transfected Hela cell? - Cell transfection (reply: 1)
  1136. How to assay the amount ofcollagen on cell/scaffold composites? - (reply: 1)
  1137. PBMC-B cell - (reply: 1)
  1138. Plant Culture Cell - (reply: 1)
  1139. stable cell line - stable cell line (reply: 1)
  1140. Cell line for glutamate receptors - Glutamate receptor cell lines (reply: 1)
  1141. lipofectin mediated transfectionof HepG2 cells - (reply: 1)
  1142. stable transfection of epithelial cells - stable transfection in mammary breast cell lines (reply: 8)
  1143. Shipping Cell Cultures - Shipping Cell Cultures (reply: 3)
  1144. using culture sup of one cell line  to induce 2nd cell line - (reply: 1)
  1145. Stable Trasfection into Hek 293and Hela cells - (reply: 1)
  1146. Isolation of nuclei from mammalian cells - (reply: 1)
  1147. Re: how to seperate deadsuspension cells from alive ones - (reply: 1)
  1148. mutation in cancer cell line - (reply: 1)
  1149. Collagen gels - Problems with growth of endothelial cells on collagen gels (reply: 1)
  1150. how to seperate dead suspension cells from alive - (reply: 2)
  1151. Yeast in Cell Culture... - How to erase the Yeast? (reply: 2)
  1152. Centrifuge cell at room temperature? - (reply: 1)
  1153. cells washed away - cell culture problems (reply: 6)
  1154. Stable Transfection of large DNAinto 293 cells - (reply: 1)
  1155. Endothelial cells culture - Protocols for the culture of endothelial cells, special tips (reply: 2)
  1156. BMP4 expressing cells - (reply: 1)
  1157. cell biology - lysate preparation (reply: 1)
  1158. Formalin fixation&paraffin-embedding of tissue culture cells - Formalin fixation&paraffin-embedding of tissue culture cells (reply: 1)
  1159. Cell wall stain - (reply: 1)
  1160. Chicken  ES cell - (reply: 1)
  1161. please help me - how to extract cd3 protein from jurkat cell (reply: 1)
  1162. Influenza inoculation of Hek293 cells - (reply: 1)
  1163. Quantifyng Mycobacterium Cells - (reply: 5)
  1164. Isolation of cytokine-secreting cells - (reply: 2)
  1165. memory cells - (reply: 4)
  1166. B cell proliferation assays - (reply: 1)
  1167. Retroviral infection of primaryCD4+ T helper cells - (reply: 1)
  1168. Dendritic cell isolation - (reply: 4)
  1169. which is more stable ineukaryotic  cell,ssRNA or ds RNA - (reply: 1)
  1170. single cell DNA isolation frommammalian cells - (reply: 4)
  1171. detection of calpain activity inthe Red blood cell and other tissues - (reply: 1)
  1172. How to transform the cells witht wo different plasmids - (reply: 3)
  1173. RNA isolation from a single cell - (reply: 1)
  1174. Competent Bascillus Cells - (reply: 1)
  1175. cell lysis - How do I decide what solution to use? (reply: 2)
  1176. adenovirus infection (293 cells)protocol - (reply: 1)
  1177. cell line with deletion in mitochondrial DNA - Molecular Biology (reply: 1)
  1178. Cell senescence markers - (reply: 1)