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Cell Related Discussions
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cell lysate colorless? - (reply: 1)
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How to prevent cell aggregation - (reply: 1)
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Problem with CD2F1 mouse CT26 cell cachexia protocol. - CT26 treated mice gained weight. (reply: 2)
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can i use antibiotics with lipofectamine - my cells have antibiotic as selective marker and need neomycin (reply: 4)
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Sp2/o Myeloma cell line - Media for growing sp2/0-Ag 14 cell line (reply: 4)
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Apoptosis by FACS - How to gate the cells by using Apoptosis kit from BD (reply: 1)
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Should i use cells healed from mycoplasma - (reply: 2)
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What's the major cell type in thymus - (reply: 1)
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How to change medium without dislodging the cells - (reply: 1)
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Cell Lysis - (reply: 2)
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Serum Deprivation - MDA-MB-231 cells - (reply: 5)
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VEGF secretion in MDA-MB-231 cell line - (reply: 3)
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THP-1 cells and 2-mercaptoethanol - (reply: 1)
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Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
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cell and tissue culture - healthy test (reply: 1)
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Need human cells for a science fair project - (reply: 4)
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What kind of cells is it? - need which counterstain? (reply: 1)
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Cell pellet after freezing - (reply: 3)
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cell culture maintainance - (reply: 3)
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pls recommend a serum independent expression promoter for mammalian cells - (reply: 1)
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Spontaneous Cell lysis after IPTG induction - (reply: 4)
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Treating cells with cytokines - Should you take out growth factors before treating? (reply: 2)
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Ways to stop the cell at G1 phase? - (reply: 2)
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Immunstaining cells fixed in 10% formalin- possible? - (reply: 8)
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difference between transformed and immortalized cells - (reply: 3)
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How many O.D. cells to load onto SDS-PAGE? - (reply: 2)
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Troubles in IL-8 measurement of tissue cells cultured with E.coli - (reply: 1)
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Mioblasts primary cell culture - counting the number of myoblasts (reply: 1)
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RNA extraction from frozen cells suspended in PBS - (reply: 2)
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Cell Clumping Problem - Cells Stick to Eachother and Not the Flask (reply: 2)
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Linear DNA transfection - Daudi cells (reply: 3)
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Dead cell staining - what kind of dye? (reply: 5)
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What exactly are CD4+3- inducer cells? - simply a specific form of "T-helper-cells"? (reply: 2)
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In vitro toxicology test by animal cell line - Looking for methods (reply: 1)
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inoculating liquid media from frozen cell stock ok? - (reply: 6)
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Cell treating - Do you... (reply: 4)
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cell lysis - (reply: 3)
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How to fixate cells onto glass microscope slide - (reply: 2)
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Is there any destructive effect on nucleic acid when harvest the cell by scrapin - (reply: 1)
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Transfected copies per cell - (reply: 1)
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RAW 264 cells - (reply: 1)
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How to concentrate retrovirus produced by Phoenix-Ampho cell - (reply: 4)
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Have I killed my cells? Doh! - (reply: 3)
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Looking for specific cancer cell line - Need a parent line with metastatic and non-metastatic lines (reply: 2)
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Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
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mesenchymal stem cell differentiation into osteoblasts (alizarin) - (reply: 2)
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Troubles in transfection methods! - All cells die before making a selection (reply: 5)
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siRNA into RAW264.7 cells? - any ideas (reply: 4)
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Heat-shock treatment problems with adherent cells - (reply: 1)
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No Retrovirus using the Phoenix - Ampho cells - (reply: 1)
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IEC-6, IL-6 and LPS - Cannot get my IEC-6 cells to secrete Il-6 (reply: 2)
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Creating stable cell lines - (reply: 4)
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Cholesterol Assay - Commerically available cholesterol assay kit for cell extracts (reply: 1)
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Can Osteosarcoma cells change their phenotype? - previously "Name this cancer cell's purpose and parts" (reply: 1)
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Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (reply: 13)
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cell culture question - passage number - (reply: 5)
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A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
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How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
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293t Cell Attachment Issues - (reply: 7)
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giant cells? - (reply: 6)
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Direct Cell Lysis in Laemmli - (reply: 1)
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Cell Morphology - (reply: 1)
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When to use PMSF in a cell lysis buffer? - (reply: 9)
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staining for receptors in cell line - (reply: 1)
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Counting B cells in hemacytometer - (reply: 2)
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Coating cell dishes with peptides - (reply: 2)
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Does it look like cell clumps/artefact/contamination? - (reply: 3)
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What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
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what cell line to use for visualizing membrane localized protein - (reply: 1)
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Define cell passage number - (reply: 2)
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THP-1 cells from Sigma - (reply: 1)
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DNA "snot" in cell lysis/protein extraction - (reply: 6)
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DNA extraction from dry cells - How to extract DNA from dry cells (reply: 9)
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H.influenzae and S.pneumoniae cell culture - H.influenzae and S.pneumoniae grow on plates but not in broth (reply: 1)
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Cells die after changing medium - Is there role of FBS? (reply: 1)
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Different cell culture plastics, problems with cell adherance - (reply: 6)
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Cytotoxicity assay with RAW 264.6 cells - (reply: 1)
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Decide which cell line to use - (reply: 3)
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Cell seeding density - (reply: 11)
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293T cells adhesion and transfection... - Looking for cell line for localization studies... (reply: 3)
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Cell culture supernatant - Is it just the media from cultured cells? (reply: 1)
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growth curve? - how to establish growth curve of specific cell line.... (reply: 1)
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transfecting human lymphoblastoid cells - (reply: 2)
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H9 Cell transfection - H9 cell transfection protocol (reply: 4)
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MCF-7 drug synergy - (reply: 4)
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How to kill bacteria in treated cells? - (reply: 6)
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cell line to use for neural regeneration? - (reply: 1)
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Does immortalization affect non-dividing short-living cells? - (reply: 3)
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Why do viable cells take up DAPI? - DAPI must only stain non-viable cells (ICC) (reply: 7)
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Quenching oxygen in cell culture - (reply: 1)
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Inhibitors in cell culture... - DMSO???? (reply: 4)
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% Cytotoxicity or Relative Cell Damage less than zero - What does it imply? (reply: 4)
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Ratio between cells and lysis buffer - (reply: 1)
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Immunofluorescense of floating cells - How to IF the floating cells during apoptosis? (reply: 4)
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What cell lines can be used to run an anti-angiogenic assays? - (reply: 1)
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NK Cell Isolation - negative selection kits - (reply: 1)
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Cell count in solution - (reply: 2)
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Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
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Cell Culture protocol by Current Protocol - free online version from invitrogen (reply: 3)
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cell cryopreservation in -80 - (reply: 2)
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Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 33)
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Cell growth inhibition by lipofectamine 2000 - (reply: 1)
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T cell activation for intracellular cytokine detection by Flow Cytometry - (reply: 1)
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Stem Cell Niche - Murine Adult hematopoietic stem cell division Niche?? (reply: 1)
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spinner flasks cell maintenance - (reply: 3)
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contamination of DH5alpha cell line - (reply: 6)
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MDCK cell experience - (reply: 2)
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Expression of EGFR in MDA-MB-435 cell line - (reply: 2)
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how to distinguish monocyte and dendritic cell under microscope - (reply: 2)
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cell cultur medium - (reply: 3)
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High-throughput screening of suspension cell lines - (reply: 2)
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Single Bacterial Cell Isolation for Comet Assay - (reply: 1)
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FACS data analysis - understanding cell quest program and reading data from it (reply: 1)
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Problems with cell lysis - (reply: 3)
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Causes for vacuoles or bubbles in cultured cells - (reply: 7)
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Differences in MCF-7's? - (reply: 2)
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what are the methods available for protein extraction from cell line? - (reply: 6)
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Cell Counting in Hemocytometer - Teach me short cut to count in this chamber (reply: 13)
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Best method to locate a receptor on breast cancer cell? - Using an AFM (reply: 2)
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the latest research trend in dendritic cells - the heat research topics related to dendritic cells (reply: 1)
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T cell proliferation assay with macrophages - (reply: 2)
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Problems when harvesting cells - (reply: 4)
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About red precipitate during passing the cell line - (reply: 2)
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housekeeping gene for B cell lines - (reply: 1)
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Who uses non-adherent cells? - like NCI-H69... are they ready to use after passing? (reply: 1)
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no pellet after centrifugation for making competent cells - (reply: 4)
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Daudi cells transfection - (reply: 1)
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Primary tracheal cell culture - (reply: 1)
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Need some advice on HeLa cells health hazards - (reply: 7)
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Reviving THP1 cells - (reply: 6)
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Protein Isolation of very low cell number... - (reply: 4)
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making INV110 competent cells - (reply: 1)
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b-mercaptoethanol and stem cells - (reply: 3)
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Cell fractionation problem - Nucleaus forms a pellet that can't be suspended (reply: 5)
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Bradford problem in cell lysates - (reply: 1)
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Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
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Medium for culturing 2 types of cell together - (reply: 2)
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Transfect competent cell? - (reply: 1)
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Need advice on counting low cell numbers - (reply: 1)
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Can I load 1N NaOH in cell culture plate? - 1N NaOH is strong alkaline, will it eat up the glass? (reply: 4)
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Why 10% serum for cell culture? - (reply: 11)
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MCF-7 & MTT assay & E-screen - (reply: 1)
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flourescence in non-transfected cells! - (reply: 4)
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% confluency question - when to transfect the cells? (reply: 2)
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Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
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Free grid image for counting cells (freeware) - simple but useful (reply: 7)
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Question on cell line - (reply: 1)
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T98G cell size - Coulter counter (reply: 1)
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TOP10 competent cell with another vector - (reply: 1)
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Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
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siRNA for retinal pigment epithelial cells - (reply: 1)
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high levles of cell death in PC12 cells - (reply: 3)
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PMA - used to differentiate THP-1 cells - (reply: 6)
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Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
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tranwells and C6 glioblastoma cell migration - (reply: 1)
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Cell culture contamination - (reply: 9)
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yeast cell cycle and temperature - FACS profile at 25 and 36 (reply: 1)
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Melanin extraction from melanoma cells, How? - (reply: 3)
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Strange black particles while growing Sf9 insect cells - (reply: 3)
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I thawed my primary cells and froze them again? serious mistake? - cell culture (reply: 5)
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FRET Imaging :Hela cells - (reply: 2)
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Jurkat Cells and Proliferation Assay - Click-iT EdU - (reply: 2)
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cell density - (reply: 2)
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3-D tissue model for endothelial cell culture - Looking for any in vitro 3-D models to grow endothelial cells to measu (reply: 1)
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How to kill yeast cells :) - chloramine or autoclaving (reply: 19)
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RNA isolation from small amount of suspension cells - (reply: 3)
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Poor ligation efficiency using Invitrogen TOP10 and XLI-blue cells - (reply: 5)
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Primary gastric epithelial cells - (reply: 1)
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Ca++ estimation in the cell - (reply: 1)
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picking a cell line to perform cell transformation assays - (reply: 2)
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cell differentiation - (reply: 2)
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Culture of Epithelial cells from Breast Milk? - (reply: 2)
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HEP G2 cells - growth medium - (reply: 2)
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poor cell growth following FBS batch change - (reply: 5)
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CHO cells medium - (reply: 5)
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Paradoxical cell count - Increase in cell count wihtout (known) stimuli (reply: 2)
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Can the current technology make a human cell solely using materials from nature? - Can the current technology make a human cell solely using materials fr (reply: 2)
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Could we grwo chinese hamster cells in untreated normal petri dishes? - (reply: 1)
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Is stem cell ethical? - Do you support or oppose it? (reply: 2)
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ES cell targeting - (reply: 2)
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vehicle treated cells? - (reply: 2)
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siRNA transfection in endothelial cells - (reply: 3)
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How to confirm whether the cell line was successfully transfected with two plasm - Co-transfection of two plasmids to 293 cells (reply: 6)
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how to test the efficiency of chemically competent cells - (reply: 2)
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gene down-regulated in cancer cell line but no proliferation inhibit ability - (reply: 3)
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sudden cell death- Please HELP - (reply: 6)
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cell adhesion problems in outer wells for transfeciton - (reply: 1)
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cell lysis buffer - (reply: 4)
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RNA isolation from small amounts of cells - (reply: 1)
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DNA extraction from PFA fixed cells - (reply: 2)
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Help of establishing stable cell line - (reply: 1)
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Cell lysis method for running cell lysate on Western blot - (reply: 4)
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Problems with making efficient chemically competent cells - (reply: 2)
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N in Cell Culture - What is the proper way to determine your "n"? (reply: 2)
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Can cell debris move? - (reply: 4)
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MLN cell isolation....Help! - (reply: 1)
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Pansorbin cells - (reply: 3)
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double transfection plus siRNA - in HEK293 cells (reply: 1)
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cell line culture - (reply: 2)
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Chromosomal aberration assay for ES cells - (reply: 1)
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Unexplained Cell Culture Death - Expertise needed (reply: 7)
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Protocols for Preparation of cell lysate from tissue sample - (reply: 3)
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Cell passage question - (reply: 4)
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Calcium Flux by FACS using Fluo-4 - Can the cells be fixed? (reply: 1)
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Cell lysate at RT - (reply: 4)
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Monocyte derived Dendritic cells - (reply: 4)
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what is the media for HUVEC cells? - (reply: 4)
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Confluent 25 cm2 flask means how many cells? - quick question, need quick answer (reply: 1)
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THP-1 serum free cells - (reply: 3)
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Can enzyme kinetics be measured in whole cell extracts? - (reply: 3)
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cell scraping - I DONT recommend it for subculturing (reply: 15)
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what kind of contamination is this? (photos inside) - contamination in cell culture with HeLa (reply: 10)
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cell lysate PAGE & blotting problem? - (reply: 1)
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Question about nontreated cell cultureware - (reply: 5)
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looking for HCV positive cell lines? - (reply: 6)
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what would happen if i dilute protease inhibitor too much in cell lysate? - (reply: 3)
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A problem about infecting primary T cells - (reply: 1)
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what to do with the differentiated mouse es cells - (reply: 3)
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What is the pI of most of proteins in cell? - (reply: 3)
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easy & quick apoptosis assay for siRNA treated cell line sample - (reply: 3)
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Loading cell pellet in SDS-PAGE - (reply: 4)
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What happen to my NIH3T3 cells? - (reply: 6)
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perovskite oxide nanowires in living cells - (reply: 1)
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Really, isn't there anyone who can recommend a TUNEL assay? - for adherent cells using fluorescent microscopy? (reply: 2)
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Fixing cells in wells? - (reply: 4)
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Problem with thawing cells from liquid nitrogen? - (reply: 6)
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Problem with mouse embryonic stem cells - contamination? - (reply: 1)
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Cell immortalize - (reply: 2)
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TUNEL assay- should I grow cells on slides? - Or label cells in suspension then deposit on slides? (reply: 1)
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confirming how the gene causes decreased growth/increased cell death - (reply: 4)
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To keep dead cells when harvesting? - (reply: 2)
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anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
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how to sdudy the matter exchanges among cells? - (reply: 4)
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RAW 264.7 cell transfection - Probs transfecting RAW cells with lipofectamine LTX (reply: 2)
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Transfecting P19 embryonic carcinoma cells - (reply: 2)
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Transfecting NS0 cells - (reply: 5)
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Calculating cell purity after MACS enrichment - (reply: 1)
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In vivo T cell depletion - (reply: 1)
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HEK cell, EGFP and PI staning - (reply: 7)
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ligation transformation.......which chemically competent cells are the best? - (reply: 3)
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irradiated 3T3 cells as feeder cells - (reply: 2)
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Is it true that a cell line cannot be infected with virus again once it was alre - virus infection (reply: 1)
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Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
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Where can we purchase human microvascular endothelial cells? - (reply: 1)
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stimulation of cell division/mitosis? - of non-dividing exocrine cells (reply: 4)
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How to make Matrigel layer as thin as humanly possible? - Our technique relies on a VERY close contact of cell with coverslip (reply: 5)
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Maintaining stable cell line - (reply: 5)
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Stable cell line - clone vs. population - (reply: 15)
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How to isolate gastric parietal cells? - Do you have protocol for isolation gastric parietal cell? (reply: 3)
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how to distinguish normal and cancerous osteoblast cells - (reply: 3)
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How to kill proliferating cells but not arresting cells? - (reply: 3)
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Cell contamination - Small rapidly moving black rods (reply: 18)
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TMK 1 Cell - Where can I buy TMK 1 cells? (reply: 1)
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Agent to punch hole in cell membranes - (reply: 1)
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Yeast Competent Cells - (reply: 34)
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cell lysate for immunoprecipitation - (reply: 2)
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Transient Transfection of HeLa cells. - (reply: 1)
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Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
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reviving corneal cell line - (reply: 1)
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Calculating cells/well in a 96-well plate - Calculating cells/well in a 96-well plate (reply: 8)
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ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
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Human dendritic cell line - (reply: 5)
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Cells dying upon temperature shift. - (reply: 7)
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Spermatogonia stem cell culture - (reply: 4)
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THP-1 cell culture problem: Cells die after thawing - (reply: 4)
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Fixation of cells for long time storage - (reply: 1)
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cell migration, cell invasion and cell proliferation assay - (reply: 5)
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cell culture - problem with suspension CHO cell revival (reply: 2)
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Optimization of seeding cells number for MTT assay - (reply: 5)
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MCF-7 cell grow too slow - (reply: 5)
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Living cell GFP imaging - (reply: 6)
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weird S2 cells - (reply: 1)
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neuronal cell suspension from brain - (reply: 4)
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Transfection of Drosophila cell culture - Which is the best transfecting agent? (reply: 1)
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A CO2 independent medium limit the range of cell types that can be investigated? - (reply: 1)
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Centrifugation speed for adherent cell line - (reply: 11)
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time required for cell recovery after thawing - (reply: 8)
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Competent Listeria Cells - (reply: 3)
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Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
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Protein extraction from animal cells - (reply: 7)
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Sepration of cells from cell debris in sample - (reply: 2)
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Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
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HBSS vs PBS to deliver compounds to cells - (reply: 3)
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centrifugation speed for fixed cell - (reply: 2)
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competent cell contamination? - (reply: 3)
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Lysis of fat cells/adipocytes - (reply: 7)
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CHO-S cell - (reply: 1)
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cell lines - (reply: 2)
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More deep ChIPping woes (Sonication Optimization) - Primary cell line.... (reply: 1)
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how many cells in a mouse spleen - (reply: 5)
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The BEST cell counting technique? - (reply: 4)
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cell harvesting; use of antibiotics? - (reply: 8)
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Best Guess:How many cells in Organotypic Slice culture - (reply: 2)
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What are the negative effects of HEPES on cells - if any - (reply: 1)
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HELP: transfection reagent for BJ cells - (reply: 1)
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Search transfection reagent for BJ cells - (reply: 2)
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Why cells are so resistant to G418? - G418 cannot kill cells (reply: 6)
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what's wrong with my MTT assay on a suspension cell line - (reply: 8)
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Ooops! I messed up! Help! - freezing cells (reply: 5)
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Co-IP in sonicated whole cell lysates - (reply: 3)
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no GFP in cells - (reply: 7)
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SLOW cell growth - (reply: 2)
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Apyrase in cell culture - Half-life? (reply: 3)
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Cells are lost after fixation - (reply: 14)
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primary sertoli cell transfection - (reply: 2)
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how to separate dead from viable cells in cultures of non-adherent cells - (reply: 3)
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Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
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Hela cell contamination? - (reply: 10)
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Cat No of M199 medium for endothelial cells URGENT - (reply: 1)
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brdu staining on gfp+ve cells - (reply: 1)
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How to infect T cells? Lentiviral or retroviral? - (reply: 2)
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dendritic cells culture - (reply: 3)
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cell lysis and save the ECM - (reply: 1)
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Harvesting cells following transfection - Scrapping or trypsin (reply: 2)
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Competent cells not growing - (reply: 5)
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preparation of bacterial cell lysate - how to do? (reply: 1)
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Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (reply: 3)
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Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
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Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
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Cell fractioning in order to perform binding assay - (reply: 1)
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competent cell didn't grow in LB - (reply: 15)
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BrDU staining on GFP positive cells - (reply: 1)
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Contamination or not? - Cell Culture Contamination (reply: 1)
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protocol for Trypan Blue exclusion assay - How to do trypan blue exclusiona ssay on adherant cells? (reply: 3)
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Infection of human cell lines grown in suspension? - Infection protocols (reply: 3)
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Need advice for growing THP-1 cells - (reply: 4)
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Live cell nuclei immunostaining - (reply: 2)
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Does a B lymphocyte contain lysosome inside? - Cell Structure (reply: 1)
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Cell fractionation-help! - (reply: 6)
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Nystatin makes vero cells round, too high concentration? - (reply: 3)
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How to separate cells in urine for FISH? - (reply: 1)
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CaCl2 Competent Cell - (reply: 3)
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culturing trouble making competent cells - (reply: 1)
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How to pick cell from soft agar and recover it? - (reply: 2)
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Schock freezing - cell disruption? - (reply: 3)
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Culturing of MUTZ-3 cells - (reply: 1)
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Adipocytes from stem cells? - (reply: 6)
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culture CD34+ Hematopoietic Stem Cells - (reply: 2)
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Preparing cell lysate from cell culture for Western blot - (reply: 4)
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How to introduce DNA to animal cells? - (reply: 3)
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MTT assay + suspension cells - (reply: 4)
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Western Blot of cells in Laemmil - (reply: 5)
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AML cell lines - Where can I get them?? (reply: 1)
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prepare electrocompetent cells - (reply: 4)
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CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
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plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
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Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
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Just how many cells are lost when changing tubes? - (reply: 5)
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Cell cycle: ModFIT vs. FlowJo - diferent percentages outcomes on same histograms (reply: 1)
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problem with plating adherent cells in 24-well plates - (reply: 11)
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Raji Cell Histone Immunocytochemistry and Confocal Help - (reply: 1)
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Cell Seeding and Drug treatment - (reply: 12)
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Embryonic stem cell culture - (reply: 4)
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cell biology video clips - (reply: 2)
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cell seeding confusion - what does it mean (reply: 1)
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Using Adenovirus to transfect cells - (reply: 5)
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Cell assay for ROS measurment - (reply: 1)
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Red Cells in Clear Ficoll Layer Following Density Centrifugation - Monocyte Isolation (reply: 2)
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preparation of electroporation competent cells from chemically competent cells - (reply: 3)
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cell culture - passage number (reply: 7)
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do the competen cells chang the U to T - (reply: 4)
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increased BrDU incorporation but similiar cell cycle profile by PI or DAPI - (reply: 2)
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E.coli TOP10 comp cells T1 Phage resistant? - (reply: 1)
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what time is better to seed cells in well or dishes before transfection? - (reply: 7)
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Experiment with KATO III cells - (reply: 1)
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SiRNA in MIN6 cells, nucleofection - (reply: 1)
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Cell passage number for non-cancerous cells - (reply: 2)
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Cell death with DMSO treatment, during transfection using polybrene - (reply: 2)
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stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
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Homework question - what happens to cells when they're put in distilled wate - (reply: 3)
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looking for protocol using DAPI for cell cycle analysis - (reply: 1)
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Synchronization of NIH 3T3 cells - (reply: 2)
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difference between staining vital cell and dead - (reply: 9)
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why should the conversion efficiency of competent cell be at least 10^8 cfu/_ - (reply: 2)
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Enlarging Cells - (reply: 3)
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Stable cell line losing expression faster than usual - (reply: 6)
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PCR from cell culture - to detect the presence of adenovirus (reply: 2)
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Passaging stable transfected cells after thawing - (reply: 4)
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Cell culture - (reply: 2)
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Is that possible to establish a stable cell line which is transfected by an empt - (reply: 2)
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Could competent cell be stored in liquid nitrogen? - (reply: 7)
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what happens if cells turn liquid? - (reply: 4)
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Cell Fixation method - % of PFA ? (reply: 4)
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culture cell - (reply: 1)
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Storing Induced E.Coli Cells - (reply: 1)
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choosing competent cells - (reply: 5)
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FACS sorting and culturing cells afterwards - (reply: 1)
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Secrets for declumping cell pellets - (reply: 5)
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Best compotent cells - (reply: 5)
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mouse U6 promotor in human ES cells - (reply: 1)
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Cell Surface Biotinylation - Advantage/Disadvantage (reply: 7)
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changed attchment of RMA/S cells after transfection, why? - (reply: 2)
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cell culture contamination - after transfection (reply: 15)
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How to prepare my own competent cells? - as above (reply: 5)
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removing rbc cells for cell purification - (reply: 5)
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cell lysis and DNA - sticky (reply: 3)
-
293T cells - (reply: 3)
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Frustrated with FACS (ONLY DEAD CELLS) - (reply: 8)
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few cells in the cell culture - (reply: 3)
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counting viable cells directly from wells - (reply: 7)
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how do i do flow cytometry for adherent cells? - (reply: 5)
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Problem with Monoclonal antibody production - why cells die after adding HAT?????? (reply: 6)
-
pBABE-puro in 293T cells (not PhoenixA) - (reply: 5)
-
stable transfections using SCC 9 cells - (reply: 5)
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Stable transfection of human adrenocortical cancer H295R cell line - (reply: 1)
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any tips for detecting phospho-ptotein in fixed adherent cells by flow cytometry - (reply: 2)
-
293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
-
How to seed adherent cells evenly in 150mm dishes? - (reply: 12)
-
PMA induced apoptosis in HEK cells - (reply: 4)
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Why different mammalian cell lysate preparation methods? - (reply: 6)
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cell lysis to investigate phosphorylated protein - (reply: 1)
-
centrifugation speed for mouse neural stem cells - centrifugation speed for mouse neural stem cells (reply: 3)
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cy5 cell tracker dye - (reply: 2)
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transformation competent cells problem - fastbac vectors (reply: 2)
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Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
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transformation of competent cells - (reply: 2)
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Test for drug dose response in suspension cells before transfection - With Suspension cells, how do people distinguish difference between de (reply: 1)
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adipose stains? - specific stain to positively ID adipose cells (reply: 2)
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Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
-
isolation of total RNA from bacteria attached to HeLa cells - (reply: 1)
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SURE E. coli strain - How make competents cells? (reply: 4)
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APC isolation from the Liver - How you can isolate the APC cells from the mice's liver (reply: 2)
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Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
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differentiated PC12 cells detatching - (reply: 3)
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Thp-1 cell transduction - (reply: 4)
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HIV-2 and MTT assay, cells lines to use - (reply: 2)
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tissue preparation for human/mouse islet cells - (reply: 1)
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Protein Extraction from cell media for western blot - (reply: 3)
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cell adhesion to plastic - (reply: 3)
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Epithelial cell-cell fusion - Protocol? (reply: 1)
-
Chemically competent cells? - (reply: 3)
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cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
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bath sonicator for cell lysis - (reply: 2)
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serum starvation - induce cilia by serum starvation in Rpe-1 cells (reply: 7)
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Keep growing cells after an MTS/PMS assay - (reply: 1)
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Cells in log phase - (reply: 1)
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protocol for transfection(eletroporation) for HEK293 cell - (reply: 1)
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Stable cell line, polyclonal and monoclonal. - Which is better? (reply: 4)
-
lentiviral transduction of suspension cell - (reply: 1)
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cell counting dilemma - (reply: 1)
-
lentiviral transduction of suspension cell - (reply: 1)
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Immunostaining on bacterial cells? - (reply: 1)
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any kit or reagent for extracting both DNA and RNA from the same cell culture - (reply: 3)
-
FACS sorting affects cell function? - FACS sorting affects cell function (reply: 2)
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cell counting calculation.... - (reply: 3)
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Phoenix 293T packaging cells - (reply: 3)
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suparnatant, cell free extract, crude extract - (reply: 4)
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competent cells transform 12 kb plasmid,urgent - (reply: 8)
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Whole cell extract on SDS/PAGE - (reply: 9)
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Making competent cells from current commercial stocks - (reply: 3)
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Calculation cell in 96 well plate - (reply: 1)
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G418 and resistant cells - can't kill the cells (reply: 8)
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MIN6 cells - (reply: 9)
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cell signaling & choice of the cell - (reply: 3)
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hard to transfect cell lines - (reply: 1)
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How can I separate nuclei from cytoplasm in trophoblastic cells - (reply: 1)
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salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
-
Primary culture - Transparent cell? (reply: 2)
-
transfection of chick cell - (reply: 3)
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non-adhernt cell washing - (reply: 1)
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Immunology on fixed bacteria - What are the basics of fixing cells for immunological analysis? (reply: 1)
-
transfection of PC12 cell line - (reply: 1)
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strong attaching epithelial cells - molecular explanations? (reply: 1)
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How to save my primary cell lines! - (reply: 1)
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My cells die on poly-d-lysine coated plates! - (reply: 2)
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isolation of celmembrane of tissue cells - (reply: 2)
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How to grow frozen DB 3.1 cells - (reply: 1)
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REH cells and knockdowns - (reply: 1)
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Cell centrifugation at 3200xg - Can cells survive such speed? (reply: 4)
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transfection with PA-1 cells - (reply: 1)
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How to separate the antigen presenting cell from spleen of rat - (reply: 1)
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Transfection of T47D cells with FuGene in 10cm plates - (reply: 2)
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Immunofluorescence staining of spheroid cells - (reply: 3)
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How small cells can Flow cytometry detect? - Flow cytometry to detect a small cell (reply: 2)
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tg1 and hb2151 cells - (reply: 2)
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How to avoid central cell seeding in 6-well plates - (reply: 19)
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Same cell line but different growth rate - (reply: 4)
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Fixing Cells for Flowcytometry - Glutaraldehyde, Formalin, Microwave (reply: 11)
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estimating number of cells to seed - (reply: 2)
-
cell dead after changing the medium to PBS buffer - (reply: 3)
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PC12 cell culture. Do I have to coat plates first? - (reply: 2)
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Can scintillation fluid be used to harvest adherent cells? - (reply: 1)
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Difference between cell invasion and cell migration - (reply: 4)
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too many dead cells why? - (reply: 3)
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Do cells transfected with lentivirus express specific unique surface markers - (reply: 2)
-
Help: Cell Lysing - (reply: 5)
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Avoiding cell clumps - (reply: 7)
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can cells just stop having the gene? - (reply: 4)
-
how important of Serum proportion of culture medium in cell culture - SOS (reply: 8)
-
question about dendritic cells and antigen-specific T cells - (reply: 1)
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CD3 and CD28 T cell stimulation in TUBES - Technical question (reply: 4)
-
cell wall protein extraction of gram-positive - (reply: 1)
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How to do the lysis of cells without white stuff? - (reply: 1)
-
cell culture - a beginner - (reply: 3)
-
any video or animation of experiments about cell and molecular biology - (reply: 1)
-
how to calculate cell number? - (reply: 1)
-
Flow Cytometry 2 Parameter Statistics, please help - Can you find standard deviation of % Positive of Total cells based on (reply: 1)
-
cell concentration calculation - (reply: 2)
-
isolating of mouse spleen Dendritic cells by nycodenz - (reply: 4)
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Suspension cell culture. - (reply: 5)
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PC12-Neurite out growth - less no of cells with neurite (reply: 4)
-
p53 independent cell death - (reply: 2)
-
Dendritic cell line media - Dendritic cell line media (reply: 1)
-
Assay for cell purity determination - (reply: 2)
-
fungal contamination - fungal in cell culture (reply: 4)
-
Double-Thymidine block of Hela cells - (reply: 3)
-
endothelial cell isolation from mouse - using magnetic dynabeads from DYNAL (Invitrogen) (reply: 6)
-
OD600 and cell density correlation? - (reply: 1)
-
T cell activation via CD3 and/or Cd28 need to solve a problem - T cell activation cd3 (reply: 5)
-
Plant cell culture- How do prevent differentiation of callus cells - (reply: 2)
-
Confocal microscopy with primary cell cultures - (reply: 8)
-
stable cell line characterization and validation - (reply: 1)
-
Trouble concentrating cell extract -please help! - (reply: 9)
-
cell synchronization - (reply: 1)
-
Proteinase k treatment of cells - (reply: 2)
-
Cell lysis and osmotic shock - (reply: 7)
-
De novo plasmid methylation in eukaryotic cells - (reply: 3)
-
Collection of selective antibiotic conc for ALL cell lines - Please add yours! (reply: 6)
-
endosome markers for mouse T cells - (reply: 3)
-
what is the mechanism of transformation of a plasmid into a bacterial cell? - (reply: 5)
-
ELISA Question - Finding the concentration of Ab from cell culture (reply: 4)
-
Lyse cells with TCA - (reply: 1)
-
separating normal epithelial cells from malignant transformed - protocol suggestions? (reply: 2)
-
a question about screening transfected cells by GFP protein - (reply: 5)
-
which -20 or -80 is more less degradation of cell lysates - (reply: 4)
-
Washing during cell staining - FACS (reply: 2)
-
How many days can fiexed cells be left before running flow - (reply: 1)
-
Trypsinizing MCF10A cells - (reply: 1)
-
transfection in NIH3T3 cells - (reply: 10)
-
double digested PCR product into competent cell - (reply: 2)
-
CAn trypsin be used for cell harvest? - (reply: 4)
-
How to make an immortalized cell line/ How to determine IC50? - Protocol for developing a drug resistant cell line? (reply: 6)
-
When are the cells virus free? - (reply: 6)
-
cell culture contamination - (reply: 2)
-
Remove BSA from cell culture medium - (reply: 5)
-
Transfection of Breast Cancer Cell Lines - (reply: 2)
-
Help! neural stem cells culture - no neurosphere after P1 - Please troubleshoot my protocol (reply: 2)
-
Apply tranfection reagents DIRECTLY onto the cells. - After removing the medium. (reply: 4)
-
aza treatment of D283 cells - aza treatment of D283 cells (reply: 1)
-
Inconsistency of the cells~~ - (reply: 4)
-
Quantifying HeLa cells in a 96 well plate - (reply: 2)
-
Epithelium cell purification - (reply: 2)
-
Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
-
Stable cell line stops growing :( - (reply: 6)
-
siRNA protocol in floating cells - (reply: 1)
-
Extraction of genomic DNA from 10 fold serial dilution of cells. - (reply: 5)
-
Basics of Cell Culture - (reply: 2)
-
if you plate 40 ul of your electrocomp cells only... - would you get any colonies? (reply: 13)
-
Explant culture - All method of cell isolation coming from animal tissue (reply: 3)
-
E.Coli. XA90 cell - transformation in XA90 cell (reply: 3)
-
cells - (reply: 2)
-
protocol for purificaiton of human mononucleus cells - (reply: 2)
-
How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
-
cell membrane - (reply: 6)
-
Endothelial cells purification - (reply: 2)
-
How to make mES cells attch to differentiate? - (reply: 1)
-
linear or supercoiled plasmid for creating stable cell line - (reply: 1)
-
why some microorganisms form filament instead of single cell? - (reply: 1)
-
can arginine be used as nitric oxide donor compound in cell culture. - kinly help!!!!!!!! (reply: 3)
-
freezing HEK293 cells - (reply: 3)
-
non-transfected cells - (reply: 3)
-
rat1 cells freezing and thawing - how to culture rat1 fibroblasts (reply: 1)
-
diluted cells too much? help - (reply: 1)
-
primary cells better than 3T3 cells? - (reply: 3)
-
Live cell transportation - (reply: 14)
-
PBS composition for HeLa cells - (reply: 2)
-
Flow Cytometry Intracellular and dead cell staining - (reply: 2)
-
single channel and whole cell - (reply: 2)
-
MTT assay of MCF-7 culture - what is duplication time of MCF-7 (reply: 3)
-
Different sizes of PCR products from different competent cells - (reply: 4)
-
Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (reply: 4)
-
cell culture - (reply: 4)
-
AzaC treatment of cells? - (reply: 2)
-
Characterization of a smooth muscle cell line - (reply: 2)
-
cell respiration - (reply: 1)
-
How to isolate intact mitochondria from live cells? - (reply: 1)
-
Curiosity: competent cell and glycerol - (reply: 7)
-
DNA extraction- bacterial cells - kits - PLease help!!! (reply: 2)
-
Is my RNA dead? - freezing cells after adding RNA lysis buffer (reply: 2)
-
mES cells cultured on uncoated dishes/flasks grow well! - (reply: 1)
-
cryopreservation agent missing in frozen cell stock - (reply: 3)
-
transfecting primary cells - (reply: 1)
-
Cell Culture Book - any suggestion? (reply: 29)
-
293F cells revival - (reply: 2)
-
Does TPA induce cell proliferation/growth? - HT1080 cells (reply: 3)
-
protocol on M cell model - (reply: 3)
-
expression problems in mammalian cells - (reply: 2)
-
transient transfection of a stable cell line? - (reply: 4)
-
I want to inqure some questions about cell lines and cell phenotype - which cell line has the highest transfection efficiency as you know (reply: 1)
-
WB with cell culture medium - (reply: 2)
-
plasmid purification from mammalian cells? - (reply: 4)
-
help- G0 synchrony of MCF10 A breast cells and MCF-7 cell lines - (reply: 1)
-
Get rid of cell debris after transformation? - (reply: 1)
-
monocytic cell line differentiation - (reply: 2)
-
Obtaining cells from human tissue samples - (reply: 2)
-
Changes on cells. - (reply: 5)
-
3T3-L1 cell differentiation - (reply: 2)
-
stable cell line questions( cell type matter) - (reply: 2)
-
Cell Culture with Human Plasma - (reply: 2)
-
cell saparation from lymph nodes and spleen - how can I improve my yield? (reply: 1)
-
G418 conc for HEK293 cells - (reply: 11)
-
Protein loading control for RAW cell SUPERNATANTS in western blot - western blot (reply: 7)
-
Transient transfection on a stable cell line - (reply: 2)
-
Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
-
Infection efficiency of non-adherent cells - (reply: 3)
-
Are there TGFB receptors in 293T cells? - ..and if you don't know, how would you find out? (reply: 2)
-
Which medium do you use to grow HEK293 cells? - (reply: 7)
-
Advantages to eukaryotic cells of having a nucleus? - (reply: 9)
-
Contamination of unique cell line (eeek!) - (reply: 5)
-
how to seperate cells from the dead and the live - (reply: 9)
-
Expression in other mammalian cells with human H1 promoter - (reply: 1)
-
Which cell lines are best? - (reply: 6)
-
Knockout cell lines? - (reply: 4)
-
If I accidentally injecting human breast cancer cell lines into my finger - What happens? (reply: 7)
-
competen cell - (reply: 3)
-
Tumour cells grow slower than normal cells, why? - cell proliferation rate in normal vs tumour cell (reply: 2)
-
paraformaldehyde solution - for cell fixation (reply: 19)
-
How to seed cells evenly in small well plates - (reply: 27)
-
remedy against cell contamination - (reply: 4)
-
stain similar to neutral red - stain unfixed cells directly (reply: 4)
-
How to lift attached CD14 cells? - (reply: 3)
-
How can i decontaminated my cell culture ? - (reply: 4)
-
Cell fixation problem - (reply: 3)
-
Hela cells and stable transfection help please! - (reply: 6)
-
Is cell culture considered in vivo? - (reply: 4)
-
Transfecting KG 1 cell - (reply: 3)
-
cell stain, nonfluroescent - (reply: 4)
-
Need to Slow down Cell Culture growth - (reply: 2)
-
What size needles I should use for cell extraction protein - (reply: 1)
-
Cell adhesion assay - (reply: 2)
-
Tetracycline in cell culture media - (reply: 5)
-
Treatment of cancer cell - (reply: 2)
-
why centrifuge before doing a cell count? - (reply: 1)
-
amount of proteins isolated from cells - (reply: 2)
-
clumping cells - s. aureus wildtyp cells (reply: 3)
-
Primary Hippocampal cell culture-DEAD! - (reply: 3)
-
How long can cells survive at 4 degree - (reply: 2)
-
Medium for adherent cells and serum/protein free - (reply: 2)
-
Mouse ES cell won't attach - (reply: 1)
-
monitor cell growth - (reply: 1)
-
RNA extraction from formaldehide fixed cells - (reply: 2)
-
vector for expression of tag on cell surface - (reply: 7)
-
Fibroblats contamination in primary cell cultures - Fibroblats contamination in primary cell cultures (reply: 6)
-
why competent cells take up only 1 type of plasmids? - (reply: 7)
-
cell lysis buffer recipe - simple question (reply: 6)
-
Help with HeLa cells culture... - (reply: 10)
-
FACSing of MCF-7 cells - (reply: 3)
-
FLAG-Tag Protein expression in MCF-7 - (reply: 8)
-
Why tumor cells are hard to culture - (reply: 3)
-
Electroporation of stem cells with DNA in Top10 - endotoxins? - (reply: 4)
-
magnetic affect of ameoba cells? - how can i make this a science fair project (reply: 1)
-
Evaluation of Boyden Chamber Assay - how to count cells? (reply: 4)
-
Cell Fixation - Ethanol or Methanol? - (reply: 4)
-
C6 Cells Contaminated - What do you think it is? (reply: 6)
-
hippocampal cell culture - I need a help (reply: 5)
-
How to make electrocompetent cells without refrigerated centrifugue - (reply: 3)
-
Bacterial cell adhesion assay - (reply: 6)
-
T cell culture - ag specific T cell culture (reply: 1)
-
ChIP from low amount of cells - (reply: 1)
-
What's difference between RPMI and DMEM - which media I should use to culture cells after transfection? (reply: 3)
-
MCF-7 staining protocol - nile red, or oil red o? (reply: 2)
-
Long-time storage of tissues/cells with fluorescent tracers - (reply: 4)
-
Transfection of 293 cells for protein expression - optimum conditions for transfection!!! (reply: 5)
-
Why is non-viral transfection efficiency of endothelial cells low? - (reply: 1)
-
Hepg2 cells resistant to trypsin digestion - (reply: 7)
-
Immunofluorescence of frozen cell culture inserts - (reply: 6)
-
T cell proliferation assay - (reply: 1)
-
SHSY5Y cell transfection - (reply: 5)
-
what is the function of the morpholinopropanesulfonic acid? - cell labeling (reply: 4)
-
mold problems in cell culture - (reply: 1)
-
Progenitor cell type - (reply: 1)
-
can the phoenix cell be transfected using lipofectamine ? - (reply: 2)
-
Increase in cell viability? - (reply: 1)
-
Making frozen cell pellets competent - (reply: 3)
-
Unhealthy Passaging? - passaging cells too much... will they go bad? (reply: 4)
-
Dead cell removal -why? - (reply: 3)
-
cancer cell transfections - (reply: 2)
-
About the cell lysis methods - (reply: 8)
-
Need prototocol for membrane fractionation from mammalian cells - (reply: 1)
-
Culturing of HEP G2 cells - (reply: 5)
-
low viability of stably transfected cell line from cryostock - (reply: 1)
-
Can I use 3H-Thymidine on adhesive cell for cell proliferation assay - (reply: 8)
-
How 2 Calculate cells/mL to cells needed per well? - EZ way to determine how much cell suspension to add/well for a certain number of (reply: 6)
-
lipid content HepG2 cells - (reply: 2)
-
M-NFS60 cell line revival - Revival protocol (reply: 3)
-
Any effect of different culture dishe size on cell? - (reply: 20)
-
doubling rate of MDA-MB-453 cells - (reply: 1)
-
Primary endothelial cells went differentiated - Medium, serum or other factors? (reply: 6)
-
Trypan blue cell death assay on cardiac myocytes - (reply: 3)
-
What is an acceptable CO2% range for growing cells in DMEM? - (reply: 8)
-
mycoplasma effects on cells - will mycoplasma kill the cells? (reply: 2)
-
method for staining of invisible cell pellets? - (reply: 1)
-
rna isolation from bone marrow cells - (reply: 1)
-
Monocyte-derived DC generation: CD14 separation or adherent cells better? - (reply: 1)
-
GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
-
transplantable rats or mice cell line in nude mice - (reply: 1)
-
How to tell cell are contaminated by fungi - (reply: 4)
-
Need protocol: CFSE Staining/ primary cells - (reply: 2)
-
why do you prepare heat-stable fractions of cells? - (reply: 1)
-
Puromycin and HEK293 cells - (reply: 3)
-
neuronal markers for the mature level for spinal cord cell - (reply: 4)
-
tissue n cell culture - (reply: 3)
-
do cells benefit from being of different age? - (reply: 2)
-
cell culture contamination - (reply: 2)
-
Can I call my cell a established cell line? - (reply: 1)
-
how long can cell lines be carried? - (reply: 5)
-
transfection of HeLa cells - (reply: 4)
-
cell stain and the fixation? - (reply: 2)
-
Cell Differentiation - Time periods and environment for proper cell differentiation (reply: 1)
-
Cell Lysis - (reply: 2)
-
Does anybody have MIN6 cells? - MIN6 Cells (reply: 1)
-
SiRNA and GFP transfection MCF7 cells - (reply: 4)
-
questions about MCF-7 cells - (reply: 10)
-
detachment of my cells?/ - (reply: 7)
-
depolymerizing microtubules in 293 cells - (reply: 1)
-
cell proliferation assays - (reply: 2)
-
Retroviral transduction of cells - troubleshooting help please! - (reply: 8)
-
After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
-
should dead cell detach? - (reply: 2)
-
how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
-
Stem Leydig cells - (reply: 1)
-
adenovirus vs. retrovirus cell infection--pros and cons? - infecting PC12 cells and overall general toxicity (reply: 3)
-
problems with Clonetech 10G cells - (reply: 1)
-
THP-1 cell line - Passage number affect phagocytosis (reply: 12)
-
MTT test for the suspension culture - Raji cell and K562 (reply: 4)
-
maximum plasmids can be co-transfected into Hela cells - (reply: 4)
-
T cell proliferation assay - (reply: 3)
-
Culture of adherent SH-Sy5y Neuroblastoma Cell Line - (reply: 5)
-
CCR5 cytometry on dendritic cells, probles with isotype control - (reply: 2)
-
transformation of competent cells with recombinant plasmids - (reply: 2)
-
plasmids smaller than empty vector - using expensive cells - still not working (reply: 1)
-
Knocking down the cPLA2,sPLA2 in Cancer cells - (reply: 3)
-
Primary cell cutures - what is this (reply: 2)
-
chemical that impede cell growth - (reply: 1)
-
EM, cell preparation: to coat or not to coat slide with collagen - electron microscopy (reply: 1)
-
microglia cell culture - (reply: 5)
-
siRNA delivery in non-dividing cells - (reply: 8)
-
PC12 cell culture - (reply: 5)
-
Turbo Competent Cells - (reply: 3)
-
yeast contamination in my cell culture - (reply: 11)
-
Cell Biologists working in 3d cell cultures - (reply: 1)
-
How to identify whether a cell line might posses stem cell characters - Any general molecular markers for stem cells? (reply: 4)
-
Use of antibiotics in cell culture? - (reply: 5)
-
how to prepare total cell lysate for Western blot? - (reply: 10)
-
doxorubicin treated cells - (reply: 1)
-
Problem with cell line/G418 - (reply: 8)
-
Chondrocytes - Need a Chondrocyte cell line (reply: 2)
-
RNA isolation from trypsinized cells - (reply: 4)
-
how to solve the problem cell culture contamination? - (reply: 2)
-
how to know that ur cells are confluent? - (reply: 2)
-
How to make a stable cell line? Help! - (reply: 3)
-
time when cells go into apoptosis - (reply: 2)
-
doxorubicin treated cell cutlures? - (reply: 1)
-
Cell line passage number? - (reply: 3)
-
basic techniques for cell treatment - (reply: 6)
-
Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
-
sodium azide on living cells? - (reply: 3)
-
Whole cell extraction buffer... - does any one have the recipe? (reply: 1)
-
Fetal bovine serum and cell culture - (reply: 5)
-
optimization of cell isolation protocol - optimization of cell isolation protocol (reply: 1)
-
Why starving cells before a tratment? - (reply: 6)
-
cell death pathways, how many? - and their basic differences.... (reply: 6)
-
Measuring cell viability via luminescence... plate type? - Promega CellTiter-Glo kit (reply: 2)
-
Functional study with PC12 cells - (reply: 16)
-
lysis of cells - how to lyse cells (reply: 6)
-
Cell line to be used for protein expression an purification - (reply: 8)
-
TLR expression on Vero cells - (reply: 2)
-
Role of DMSO in Cell fusion. - (reply: 2)
-
Need protocol for mouse ES cell stable transfection - (reply: 1)
-
does phenol:chloroform extracted plasmid creats problem in Mammalian cell cultur - (reply: 5)
-
in vitro cell proliferation assay - use of the DMSO as drug solvents (very urgent) (reply: 3)
-
Are transiently transfected DNA methylated in mammalian cells? - (reply: 3)
-
why transfected cells has less RNA? - (reply: 2)
-
methods of inducing stress in cell culture - (reply: 8)
-
Transfectiion of H295R cells by Fugene6 - (reply: 8)
-
Mammalian Cell adhesion and proliferation on biomaterials - (reply: 1)
-
removal carbohydrates cell surfaces - how to "shave off" sugars from cell surfaces (reply: 1)
-
siRNA transfection of MIN6 cells - (reply: 2)
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Is it possible to harvest E.coli cells for purification of DNA from LB agar and - (reply: 5)
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Cells survived after G418 selection are transfected cells? - (reply: 2)
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Saos-2 cells transfection - (reply: 1)
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how to make neutrophil cell lysates for Western blot? - (reply: 3)
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getting rid of shaky hands during cell culture/pipetting - (reply: 9)
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Mouse endothelial cell line - (reply: 1)
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MCF-7 growing in suspension - (reply: 4)
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infected cell line? - fungal contamination? (reply: 5)
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Growing cell lines from frozen tissue samples - Is this even possible??? (reply: 8)
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Cell Growth - Help! (reply: 7)
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Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
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Trypsin:EDTA cell detachment process - (reply: 6)
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M17 cell transfection - (reply: 1)
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Stem cell tranfsfection - Electroporation or ca-phos precipitated DNA? (reply: 7)
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Cell Viability VS Cell Proliferation assays - (reply: 2)
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crude cell lysate - storage of crude cell lysate (reply: 3)
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CHIP - Harvesting cells by trypsin - (reply: 1)
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Cell passage nomenclature - (reply: 4)
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Can DH5α be the host cell of the PCR-XL-TOPO from Invitrogen? - Help me (reply: 4)
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Techniques for counting apoptotic cells - (reply: 6)
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Persistent cell culture contamination by fungi and bacteria - Cell culture, cell biology (reply: 6)
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Adult stem cell differentiation - (reply: 2)
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Cell Lysates for western - (reply: 4)
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transfecting DNA into Jurkat cell - (reply: 1)
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single cells from human tissue? - need protocols (reply: 1)
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Tryphan Blue staining for cell viability - (reply: 1)
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Anyone has experience to grow sespension 293 cells? - (reply: 1)
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Cornea Epithelial Cell culture - (reply: 1)
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what kind of adherent cell lines can be analyzed by FCM through Annexin V/PI sta - (reply: 2)
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cell cycle data normalization - (reply: 1)
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Why are lysosomes considered the cell's "cleanup crew"? - i need help (reply: 1)
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Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line (reply: 3)
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Can I extract nuclear proteins in transfected cells? - (reply: 2)
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Ordering CHO-K1 cells - (reply: 1)
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why my IFN gamma treated cells still high in TER? - (reply: 1)
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co-transfection in NRK cells - (reply: 1)
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293T cell line, how to prevent cell clusters? - (reply: 1)
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Cell cycle analysis - Include floating cells or not?? (reply: 7)
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Death of Cells After Defrosting - MDBK cells not surviving (reply: 2)
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Extensive cell death after transfection in a 24-well plate - (reply: 3)
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Hela cells clumping in center of well of 96well plate. - (reply: 1)
-
mouse B cells - (reply: 1)
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reasons for apoptosis in primary cell culture? - (reply: 2)
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Problems with cell lysate - (reply: 4)
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competent cells - making compentent cells (reply: 2)
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Establishment of human embryonic stem cell - (reply: 3)
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Help: got the stable cell line without my target gene - (reply: 3)
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Sticky cells - (reply: 3)
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ask for 721.221 cell line - (reply: 3)
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Spectrophotomery of cell 550 vs. 600 - spectrophotometry for cells (reply: 2)
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extract peptides from tissue/cell culture for ELISA kits - (reply: 3)
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looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
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endothelial cells transfection - (reply: 2)
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How many passages cells lines can survive before senescence? - (reply: 8)
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RNA extraction from small number of cells - (reply: 3)
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NMDA tox and Rat Cerebellar Granular Cells ! - NMDA tox assay for Rat CGC's ???? (reply: 3)
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Host cells for pBELOBAC11 - (reply: 4)
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URGENT-Selecting for transformed bacteria cells - (reply: 1)
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mammalian cells invaded by intracellular microbes - how can I decontaminate? (reply: 3)
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RAW 264.7 cell line transfection - (reply: 1)
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change adherent cells into suspenion cells - (reply: 1)
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detection of GFP fluorescence in whole cell bacteria - (reply: 1)
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How to fix and stain lymphoblastoid cell onto glass slides - (reply: 5)
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no growth on plate agar for competent cell - (reply: 6)
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starvation medium recipe for Hela cells - (reply: 2)
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Inturpreting cryopreservation data - I need help applying data I'm colleting with the freezing of E. coli cells t (reply: 1)
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transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
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siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
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problem transfection Hela cells - (reply: 8)
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MCF-7 Cell line - (reply: 6)
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Switching CHO cells media from high to low glucose - how would that effect cells? (reply: 1)
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live cell confocal microscopy - protocol please? (reply: 2)
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Will low concentration of ethanol affect cell line? - (reply: 1)
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Isolation and culture of white blood cells. - (reply: 1)
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Neuro2A cell culture Death and Debris - Problem keeping cells alive for more than a week. (reply: 2)
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Human Neural Stem Cells wanted - (reply: 1)
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plating cells for transfection - (reply: 2)
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cytotoxicity and cell proliferation assay - preference of dyes for cell viability (reply: 2)
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protocol for electrocompetent cells preparation - (reply: 5)
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Transient transfection on a stable cell line - (reply: 6)
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especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
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need help for culture protacal of new stem cell - (reply: 5)
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Activating human T cells - (reply: 8)
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Storage of whole cell lysates - How? (reply: 2)
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Why use PBS before trypsinizing cells - (reply: 4)
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Question about doing RNAi in primary cells - (reply: 3)
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A Procedure for Freezing and Thawing Bacterial Cells - (reply: 2)
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Detecting proteins in Mouse embryonic fibroblast - Any tips on making cell lysates of MEFs? (reply: 2)
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Cell Tracing Software - Searching for a quicker way!!! (reply: 2)
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cells going away - (reply: 3)
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pituitary cell lines sex - (reply: 1)
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Raji cell subculture - Culturing Raji cells: DMEM or RPMI? (reply: 4)
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How to tell if competent cells or SOC are contaminated? - (reply: 14)
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Transcient transfection of HaCaT cells - which is the most effective method? - (reply: 2)
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Dissociation of HaCaT cells without using trypsin - will PBS/EDTA work? - (reply: 4)
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Where to get Granta 536 Cells - (reply: 4)
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Moving Dots in cell culture - Contamination? - (reply: 7)
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Drosophila cell culture - (reply: 3)
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Culture problems of CRL-2331 cells (ATCC) - (reply: 3)
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electroporation of PC12 cells - (reply: 1)
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RNA prep (Trizol) with small cell numbers - How low can you go? (reply: 13)
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Slow growing cells - (reply: 6)
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About cell diameter - Help,please browse (reply: 1)
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Protoreaction of Protoplasm - New approaches to Cell Biology and Physiology (reply: 1)
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Problems with Ba/F3 cell culture - (reply: 1)
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Problem growing dh5alpha cell - (reply: 2)
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Cells ignore my cover glass, growing around it on the plastic - What cower glass should be? (reply: 1)
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DH5 alpha competent cells instead of M15? - competent cells for pQE30 construct (reply: 2)
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Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy? (reply: 2)
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Transfection of coronary artery endothelial cells - (reply: 1)
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media for revival of cell lines - (reply: 1)
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transfection in eukaryotic cells - (reply: 1)
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stable transfection of MDAMB231 and Malme3M cells. - (reply: 1)
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DNA fragmentation in apoptotic cell - (reply: 1)
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Inactivated/activated FBS - is it critical for healthy cells (reply: 7)
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Adding H2O2 to cell culture - How to calculate? (reply: 4)
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cell line for lentivirus titration - (reply: 1)
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ask some question about cell size of brain - help (reply: 2)
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Role of PBS in cell culturing - (reply: 1)
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How to culture rat dorsal root ganglia cell? - (reply: 1)
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Protocal for DAPI/ Hoechst staining adhering cells - (reply: 3)
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how 2 quantify bacteria cells? - (reply: 4)
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Shipping cells in suspension culture - (reply: 6)
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hela cells - (reply: 12)
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cre recombinase - in cell lines (reply: 2)
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siRNA transfection reagent for primary cultured cells - (reply: 2)
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pcDNA 3.1/pTarget and stable cell line - (reply: 10)
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Cells are stuck - (reply: 4)
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Serum for cell culture - difference on regular vs dialyzed vs heat-inactivated FBS (reply: 3)
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Red Blood Cells exploding ! - (reply: 2)
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How does the cells of the innate immunity gets rid of the pathogens? - (reply: 4)
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Baculovirus Expression - Sonicate infected cells? (reply: 2)
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Synchronize hela cells with double thymidine block - mechanism of double thymidine block (reply: 1)
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house-keeping primers for Cos7 cells - (reply: 4)
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RAW264.7 Cells .. anyone familiar with this cell line? - need help please (reply: 2)
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adapting cells to CO2-Independent Medium - (reply: 1)
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DNA methylation in Raji cell line - (reply: 1)
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How long does take the cell cycle in HepG2 cell line? - (reply: 1)
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T cell activation. - (reply: 1)
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genetic markers for cell lines - (reply: 1)
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Needing a consistant cell suspension - Non-homogenous cells make me crazy! (reply: 2)
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transfecting HeLa cells for the first time... - help needed....please (reply: 4)
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bisulfite sequencing: cell lines vs tumors - (reply: 3)
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cell type specificity ? - (reply: 1)
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Passage number and behaviour of cell line - (reply: 10)
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what's the difference between 293FT and 293T - stable cell lines from 293Ft or 293T cell lines (reply: 1)
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Serum starvation of cells - (reply: 2)
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Transfection - Hepg2 cell lines (reply: 2)
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change in cell morphology in culture - cell culture (reply: 2)
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Has anyone tried to sonicate Jurkat cells for ChIP assay? - (reply: 7)
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macrophage cell line - (reply: 3)
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Suspension Culture - 293H cells clumping (reply: 2)
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My competent cells are misguiding me - (reply: 3)
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Did anyone ever do DCF assay using M17 cell line? - For help (reply: 1)
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Cell Membrane Permeability - (reply: 1)
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DRG cell culture - (reply: 6)
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nuclear extract - protocol for cell fractioning (reply: 1)
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Question about stem cell attachment - (reply: 1)
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maximum passage number for T47D cells - (reply: 2)
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transfection of Hek 293A cells - (reply: 5)
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cell scraper - (reply: 4)
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Transfection of cells by viral DNA - (reply: 4)
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neural progenitor cell death in multi-well plate - (reply: 10)
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Hybridoma Cell lines - How to do it (reply: 1)
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more contamination owes - spherical cell like contamination (reply: 8)
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C2C12 cells model for differentiation - (reply: 1)
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Resuspending mycobacterial cells? - (reply: 2)
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Glycerol gradients - glycerol gradients for cell extract fractionation (reply: 2)
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Fibroblast and epithelial cells in tumor samples and primary cultures - (reply: 2)
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DNA Repair in different cells - (reply: 2)
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Getting crazy because of cell contamination - (reply: 27)
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lncap cell culture - (reply: 3)
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cell fractionation plus IP - (reply: 12)
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Lab dealing with factors affecting cell membrane permeability - (reply: 1)
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passaging 293T cells - (reply: 5)
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lysosomes in cells - (reply: 2)
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Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
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making more chemically competent cells - (reply: 6)
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Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
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anyone has EcR293 cells? - (reply: 5)
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Searching specific Cell line - (reply: 1)
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Help me....About the 3T3-L1 Cells - (reply: 2)
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Mouse ES cell culture - ES cell culture for the purpose of gene targeting (reply: 3)
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HeLa in suspension culture - I want to keep HeLa cells in suspension culture (reply: 2)
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How many cells (nuclei) per ml pellet? - (reply: 3)
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Glycyrrhizin (GL) and cell culture - (reply: 1)
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transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
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ES and MEF cell - (reply: 1)
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How to reduce cell clumping for immunocytostaining - (reply: 1)
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primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
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ATCC cell lines - How is your experience? (reply: 1)
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siRNA transfection of non-adherent lymphoma cells - (reply: 2)
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need help on learning cell and tissue culturing - (reply: 6)
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can I re-freeze the 4% paraformaldehyde for longer storage? - leave cells in 4% paraformaldehyde 4 FACS? (reply: 3)
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Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
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Silly question: is DMSO sterile by itself, to add it to cells? - Lab stock bottle could be used? (reply: 6)
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Cell clumping during thawing - (reply: 2)
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mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS (reply: 1)
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what's the best system to get siRNA into a cell? suggestions - (reply: 1)
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Lysis buffer - muscle cells lysis (reply: 1)
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Help with defective cell lines - (reply: 1)
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How to measure neuronal cell differentiation? - (reply: 1)
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Cell Fusion - Fusing spleen cells with myeloma cells using PEG (reply: 1)
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Cell Death. optical spillovers.alternative assay - Need an assay for apoptosis (reply: 1)
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Fixing cells for performing immunoflourescense? - Help with protocol (reply: 3)
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Difference between cell proliferation and cytotoxicity kits? - (reply: 2)
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Bioruptor for sonication in mammalian cells - (reply: 2)
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Who killed my cells??? - Toxicity during transfection (reply: 3)
-
Transfection reagent for HT-29 and HCT-116 cells - (reply: 4)
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MCF-10A transfection question - what to do with slow growing cells. . . (reply: 1)
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FACS - permeabilization of cells without killing (reply: 1)
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DC harvesting for FACS - dendritic cells (reply: 2)
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about cell sonication - (reply: 2)
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Subculturing cells grown in serum free media - (reply: 2)
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How to screen mammalian cells clones? - (reply: 3)
-
Commonly used endothelial cells - (reply: 2)
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Primary Cell Culture - Tips for PHK culture (reply: 2)
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Primary endothelial cells not attaching - (reply: 5)
-
HepG2 cell line - what is the doubling time for HepG2 cell line? (reply: 11)
-
Has anybody generated stable cell lines using HepG2 cells? - Stable cell lines (reply: 5)
-
calculating mean doubling times for adhesion cells - Mean Doubling time for adhesion cells (reply: 1)
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Cell attachment - cells and jam (reply: 1)
-
Cell count on MCF-7 - (reply: 5)
-
5-AZA/TSA upregulation of gene in cell lines - Gene induction (reply: 3)
-
A quick question about sonication to lyse cells - (reply: 4)
-
siRNA in Drosophila S2 cells? - (reply: 4)
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extract RNA from frozen cells - (reply: 6)
-
Function of beta-mercaptoethanol in cell culture medium - (reply: 6)
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Black Debris in T-Cells - debris in my cell culture (reply: 3)
-
cell cycle analysis of transiently transfected cells - (reply: 1)
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Storage of cell lysate - (reply: 7)
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cancer cells on agar - growing adherent cancer cells on agar plate (reply: 2)
-
Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
-
Need advice on FACS for cell cycle analysis - (reply: 3)
-
Extracellular Matrix - 3t3 cells lay down ecm? (reply: 2)
-
kato-III transfection - Help with transfecting semi-adherent Kato-III cell (reply: 1)
-
DNA Sonication of quiescent cells - (reply: 1)
-
dendritic cell isolation - cell culture (reply: 1)
-
Morphologic changes of HeLa cell after RNAi - (reply: 7)
-
MCF-7 cell line with fibroblast - (reply: 1)
-
Apoptosis and Annexin V - Protocol for cell detatchment ? (reply: 3)
-
cell immunofluorescence - (reply: 14)
-
How to buy MCF-7/ADR? - (reply: 2)
-
assays to assess cancer cell growth? - (reply: 4)
-
Transforming DH5 alpha cells with pUC19 vector - Cloning (reply: 3)
-
Insect cell sonication protocol? - Protein Purification (reply: 1)
-
Fixation of cell culture with Formaldehyde - need protocol (reply: 3)
-
best cell colture media for in vivo imaging - (reply: 3)
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A question about cell stain: fix the cell have fusion protein - (reply: 1)
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How to get rid of contamination in stable transfected cells - (reply: 3)
-
how can i culture virus in primary cell lines? - microbiology (reply: 1)
-
coatings for growing cells on glass coverslips - tissue culture and confocal microscopy (reply: 4)
-
can i freeze cell lysates for western blotting? - (reply: 3)
-
real-time RT-PCR one cell - (reply: 2)
-
Screening stably transfected cells? so little? - (reply: 4)
-
confocal microscopy, fluoro beads, 3 types of cells on one slide - (reply: 1)
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Cell cycle question - CKIs - (reply: 2)
-
Tansfection in drosophila S2 cells with pEGFP-N1 - pEGFP-N1 vector @ drosophila S2 cells (reply: 1)
-
Definition of cell confluency - (reply: 6)
-
Troubles with labeling epithelial cells with CFDA-SE - Troubles with labeling epithelial cells with CFDA- (reply: 1)
-
hybridoma cell question - (reply: 4)
-
differentiate THP-1 cells - differentiate THP-1 cells (reply: 4)
-
How to sort real GFP positive cells? - autofluorescence VS GFP (reply: 2)
-
Do you reuse cell culture vessels? - (reply: 3)
-
more help: cell culture problem - (reply: 1)
-
cell cycle of NIH3T3 - cell cycle of NIH3T3 (reply: 1)
-
Osmolarity of the mammalian cell medium - (reply: 2)
-
Transferring cells from a 96 to 24 well plate - (reply: 1)
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Cell counting - (reply: 3)
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RCF safe for mammalian cell - (reply: 1)
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Incubate suspension cells? - (reply: 1)
-
Sludge when growing insect cells - (reply: 1)
-
Trypan blue cell counting - (reply: 7)
-
S2 cell expression - Protein expression (reply: 2)
-
How do you differentiate Hep G2 cells? - Hep G2 cells (reply: 6)
-
How to make a stable cell line using suspension cells - (reply: 5)
-
Frozen transformed bacterial cells problems - (reply: 6)
-
does fungi swim or move? - cell culture contamination (reply: 8)
-
Cell culture contamination - small black adherent dots!! (reply: 4)
-
passage number in mammalian cells - (reply: 9)
-
freezing of cells - (reply: 9)
-
how to prevent HEK293 cells from coming off the plate - (reply: 4)
-
transfection problem---SOS! - why the cells should be split before transfection? (reply: 7)
-
Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
-
MDA-MB-231 cell culture - (reply: 7)
-
Preparation of competent cells of pichia - (reply: 1)
-
Problem in culturing PZ-HPV-7 cell line - (reply: 4)
-
Murine markers on hematopoietic stem cells (CD34, Sca-1, etc) - Looking to identify markers on megakaryocytic progenitors (reply: 2)
-
breast primary culture cells - (reply: 2)
-
filtering for cell culture stuff - filter cell culture (reply: 5)
-
transfection of single copy of plasmid per cell - (reply: 3)
-
looking for cell line: CEMX174(T1) - (reply: 2)
-
SF9 cells - (reply: 2)
-
Natural Killer cells - (reply: 1)
-
Stable Transfection in Sf9 cells - Stable Transfection in Sf9 cells (reply: 5)
-
B-gal staining of cells - (reply: 1)
-
Need HeLa cell extract prepartion protocol - wait your answer online (reply: 1)
-
bands from tissue one size, from cells - size doubles - help! (reply: 4)
-
Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
-
red blood cell lysis - (reply: 6)
-
RNAi in Jurkat cells - (reply: 3)
-
Transfection - Cell death after transfection - (reply: 6)
-
FACS analysers - Purchase of a FACS analyser and cell sorter (reply: 3)
-
Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)
-
co-culture of two adherent cells - (reply: 4)
-
Cell lysis when isolating dendritic cells - (reply: 1)
-
hep-2 & Hep-G2 cell line - difference between hep-2 & Hep-G2 cell line (reply: 22)
-
Cell contamination, needs the help urgently - How to remove? (reply: 2)
-
EDTA only for cell dissociation - (reply: 7)
-
where to buy sterile grease used for isolate cell clone? - (reply: 2)
-
please recommend me a RNA isolation kit - RNA isolation from 100 tumor cells (reply: 1)
-
Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
-
RT-PCR of different cell lines - (reply: 1)
-
cancer cell culture - protocols on cancer cell culture (reply: 8)
-
Mammalian cell line - monolayer - monolayer growth of mammalian cell line (reply: 1)
-
How many cell passages to be safe ? - (reply: 8)
-
Contamination issue in cell culture - (reply: 109)
-
Visualizing Cell Pellet - (reply: 1)
-
LCL B cell culture - Death within 24 hours of thawing - LCL b cells are dying within 24 hours of thawing. (reply: 3)
-
Stable transfection of S2 cells - (reply: 1)
-
ATDC5 cell line - i am looking for this cell line (reply: 2)
-
Dendritic cell -isolation ---viabillity is very low - Immunology (reply: 3)
-
estrogen and MCF-7 proliferation assay - (reply: 2)
-
cell fixation - How to fix cells? (reply: 10)
-
what types of cells is a mammalian cell? - (reply: 3)
-
Help with stable cell lines - (reply: 3)
-
cell titer glo - relative light unit (reply: 1)
-
non-adherent cells immunostaining - lymphocytes immunocytochemistry (reply: 3)
-
Equipment requirements to culture cells - information needed on kidney cells (reply: 2)
-
How little competent cell do you use for transformation - (reply: 2)
-
Freezing and thawing of cells - (reply: 112)
-
Human Macrophage cell line - quick help please - (reply: 3)
-
cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
-
Primary epithelial cell line - Isolation of epithelial cells (reply: 3)
-
Cell Biology - protocol for reactive oxgyen species (reply: 1)
-
Plant cell preparation - Flow cytometry (reply: 1)
-
poly(A)-RNA extraction from cells - (reply: 1)
-
Transcriptional activity in Stable cell line. - (reply: 3)
-
A question about adenovirus infection 293 cells - (reply: 5)
-
Methods for mechanical disruption of cell - (reply: 3)
-
Cell disruption without sonication - (reply: 4)
-
pBABE Comp. Cells - Special cells for transformation (reply: 3)
-
Any ideals for cell fragmentation? - (reply: 2)
-
cell lysis - How to lysis cells (reply: 2)
-
DMSO and cell cycle - (reply: 2)
-
cell culture RNA extraction - cell culture RNA extraction (reply: 4)
-
Origami B cells grow too slowly!! - (reply: 1)
-
about LNCap cell lines - (reply: 1)
-
CBP expression problems in mammalian cells - (reply: 1)
-
problem by using percoll to purify primary cell - (reply: 1)
-
the host cell of plasmid:pBmIEGLacZ-BmIEGhyg - (reply: 1)
-
T cell proliferation - problems (reply: 1)
-
lymphocytes' RNA extraction - How many cells (reply: 1)
-
MCF-7 - (reply: 1)
-
4 layers instead of a monolayer in cell culture - (reply: 2)
-
Cell staining - (reply: 1)
-
How to keep the shape of transfected Hela cell? - Cell transfection (reply: 1)
-
How to assay the amount ofcollagen on cell/scaffold composites? - (reply: 1)
-
PBMC-B cell - (reply: 1)
-
Plant Culture Cell - (reply: 1)
-
stable cell line - stable cell line (reply: 1)
-
Cell line for glutamate receptors - Glutamate receptor cell lines (reply: 1)
-
lipofectin mediated transfectionof HepG2 cells - (reply: 1)
-
stable transfection of epithelial cells - stable transfection in mammary breast cell lines (reply: 8)
-
Shipping Cell Cultures - Shipping Cell Cultures (reply: 3)
-
using culture sup of one cell line to induce 2nd cell line - (reply: 1)
-
Stable Trasfection into Hek 293and Hela cells - (reply: 1)
-
Isolation of nuclei from mammalian cells - (reply: 1)
-
Re: how to seperate deadsuspension cells from alive ones - (reply: 1)
-
mutation in cancer cell line - (reply: 1)
-
Collagen gels - Problems with growth of endothelial cells on collagen gels (reply: 1)
-
how to seperate dead suspension cells from alive - (reply: 2)
-
Yeast in Cell Culture... - How to erase the Yeast? (reply: 2)
-
Centrifuge cell at room temperature? - (reply: 1)
-
cells washed away - cell culture problems (reply: 6)
-
Stable Transfection of large DNAinto 293 cells - (reply: 1)
-
Endothelial cells culture - Protocols for the culture of endothelial cells, special tips (reply: 2)
-
BMP4 expressing cells - (reply: 1)
-
cell biology - lysate preparation (reply: 1)
-
Formalin fixation¶ffin-embedding of tissue culture cells - Formalin fixation¶ffin-embedding of tissue culture cells (reply: 1)
-
Cell wall stain - (reply: 1)
-
Chicken ES cell - (reply: 1)
-
please help me - how to extract cd3 protein from jurkat cell (reply: 1)
-
Influenza inoculation of Hek293 cells - (reply: 1)
-
Quantifyng Mycobacterium Cells - (reply: 5)
-
Isolation of cytokine-secreting cells - (reply: 2)
-
memory cells - (reply: 4)
-
B cell proliferation assays - (reply: 1)
-
Retroviral infection of primaryCD4+ T helper cells - (reply: 1)
-
Dendritic cell isolation - (reply: 4)
-
which is more stable ineukaryotic cell,ssRNA or ds RNA - (reply: 1)
-
single cell DNA isolation frommammalian cells - (reply: 4)
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cell lysis - How do I decide what solution to use? (reply: 2)
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cell line with deletion in mitochondrial DNA - Molecular Biology (reply: 1)
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