MCF-7 growing in suspension - (May/04/2006 )
We have tried to grow MCF-7 cells from frozen several times now; frozen in 20%FBS media + 9%DMSO as done previously; -80 o/night then transferred to liquid nitrogen; thawed rapidly adn viability shows ~60% viable cells...
However after 2*passages the cells are growing rapidly but not adhering
Has anyone else had similar problems?
I've been having similar problems with cells (NCTC clone 1469) I ordered from ATCC. They grow well and the cells seem to be viable, but very few are adhering ... most are growing in suspension.
What medium do you use?
I've never had that problem with my MCF-7 cells, I use a RPMI 1640 medium with 10%FCS, 10ml pen/strep and 10ml Na-Pyruvate.
do you coat your plates/flasks to make the cells adhere better? I never have, but could it be worth a try?
I froze my MCF-7 with 10%DMSO in pure FBS. So far have't got any problems with its viability and attachment. Hope this will help.