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MCF-7 staining protocol - nile red, or oil red o? (Sep/10/2006 )

I was wondering if anyone had a really good protocol on fixing and staining MCF-7s. I want to use nile red, and oil red o. I've got a couple of vague protocols, (but given my lab doesn't have the best track record of cell staining), i'd really appreciate it if anyone had a good protocol that has worked for them in the past. Any hints or suggestions are appreciated.


hey did you get anyting on the nile red?
could you sned anyting on to me with that protocol?


check your pm. it's an easy protocol, for FACS and for slides.