cell culture Methods, Protocols and Troubleshootings

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Cell culture Related Discussions

immunostaining of cells in culture - (reply: 1)
healthy stem cells vs cancer stem cells - (reply: 1)
values for mRNA molecules per cell - (reply: 1)
Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
MCF10A Tet-ON stable cell line generation - (reply: 1)
Primary cell line - (reply: 2)
How to decontaminate adherent cells with gentamicin - (reply: 2)
Contamination in CHO cell ? - (reply: 2)
Thawing Hek 233t cells- but cells dying!! - (reply: 3)
How to purify RNA from a handful number of cells? - (reply: 5)
Check cell viablity after FACS - (reply: 1)
ISH on fluorescing cells - (reply: 1)
Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
Protein extraction from dead cells - (reply: 1)
Cells in suspension vs. plated - (reply: 2)
PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 2)
Hepg2 cells growing on top of each other - (reply: 1)
urgent! calculating concentration of compound with different cell amounts - (reply: 3)
h9c2 cells culture - (reply: 3)
Does anyone have experience of subjecting cultured cells to mechanical stress? - (reply: 1)
Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
cell population doubling - (reply: 1)
Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
Cell line licence - (reply: 1)
adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
temperature tolerance of animal cells - (reply: 2)
only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
Human insulin for cell culture? - (reply: 2)
violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
Competent cells question: JM101 vs DH5alpha - (reply: 2)
Trouble producing stable cell lines - (reply: 2)
Infecting cells with virus - (reply: 1)
Requesting basic protocols on cell sorting - (reply: 1)
lysing mammalian cells in sds - (reply: 3)
Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
Is it possible to assay the carbohydrate profile of a cell? - (reply: 2)
changing cell culture media - (reply: 1)
Preparing Palmitate:BSA solution for treatment of cells? - (reply: 6)
trypsinization of adherent cells - (reply: 7)
Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
How to lysis cells for an RNA extraction - (reply: 1)
Problem with THP-1 cell line - (reply: 3)
normalization to protein content or cell number - (reply: 2)
detection of phosphorylation from cell pellet - (reply: 2)
are THP-1 Cells(Monocytes) or Macrophages resistant to Mycoplasma infection or - (reply: 4)
Recommended competent cell for transformation of large plasmids? - (reply: 4)
Cell culture shared with yeast? - (reply: 2)
E. coli cells refuse to grow! - (reply: 2)
how to fraction stem cell at different cell cycle stage - (reply: 2)
Making Nuclei From Mouse Cells - (reply: 1)
Different cell lines different protein stability - (reply: 1)
Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
Immunofluorescence Staining of Cells - (reply: 1)
Nucleus fills the whole cell - (reply: 3)
Generating stable cell line with linear DNA - (reply: 3)
isolating cells from Cockroach - (reply: 2)
Question about suspension cells - (reply: 7)
Calu-3 cell line do not grow properly - (reply: 1)
Detach cells and stop stimulation simultaneously- How? - (reply: 4)
Counting and plating cells - (reply: 3)
cells die 4-6 days after thawing - (reply: 8)
what is the trypsin X for subculturing - (reply: 1)
Cell Envelope Proteins Smeared - (reply: 1)
Calculating the amount of the desired cells in the medium ? - (reply: 3)
long term storage of cells at -80 - (reply: 1)
Trypan Blue Exclusion Test - (reply: 2)
Making BL21 competent cells - (reply: 2)
cell plating density - (reply: 2)
What kind of cell culture contamination is it? - (reply: 8)
Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
to treat cells with inhibitor - (reply: 2)
lysis buffer not lysing adherent cells? - (reply: 8)
Trypan Blue Staining - (reply: 3)
Culturing cells on a slide - (reply: 5)
Culturing cells on a slide - (reply: 3)
protocol for immunostaining primery cells - (reply: 5)
How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
Could the use of different FBS affact my cells - (reply: 5)
Analysis of high throughput screen for cell surface markers - (reply: 1)
Lymphoblastoid cell culture - (reply: 9)
SF9 Cells lose exponential growth, lack stationary phase - (reply: 3)
Don't know why my cells are unhealthy - (reply: 2)
Hybridoma cells not secreting antibody - (reply: 3)
Determining gene of interest from Cell culture lines - (reply: 2)
Three clones of a cell line? - (reply: 3)
Freezing cells in DMSO straight into minus 80? - (reply: 4)
MCF7 cells vitality - (reply: 3)
Ultrasonic bath for cell lysis - (reply: 1)
any methods to evaluate the attaching ability of suspension cells? - (reply: 6)
Vascular Smooth Muscle cell Marker - (reply: 3)
Foam in Cell Culture - (reply: 1)
Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
SURE cells - (reply: 1)
DT40 Cell Synchronisation - (reply: 2)
Bio-Rad Cell counter - (reply: 3)
SKBR-3 cells dying - (reply: 3)
New cell culture user with no teacher or trainer - (reply: 5)
Help with some problems with Culturing Epithelial Cells - (reply: 5)
NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
What are these large multinucleated cells??? - (reply: 1)
Searching for a live cell staining method - (reply: 1)
WT BRAF/NRAS mouse Melanoma cells - (reply: 2)
HEK 293 cells Detachement - (reply: 8)
Cell culture in candle jar - (reply: 2)
centrifugation speed/cell prep for flow - (reply: 2)
Huge background with HT22 cells phophoERK antibody - (reply: 3)
Huge background with HT22 cells phophoERK antibody - (reply: 1)
Competency cells analysis - (reply: 1)
How many cells needed for co-IP and mass spec - (reply: 4)
Performing MTT assay in suspension cells (new in the subject) - (reply: 3)
cells die after transfection - (reply: 1)
Seeding cells on small metal screws - (reply: 1)
Cell lysate turbidity upon long storage - (reply: 3)
Host cells for transformation - (reply: 3)
Splitting or feeding cells for the weekend - (reply: 1)
Black moving particles in the cell Culture - (reply: 2)
lymphocyte proliferation using trypan blue - (reply: 2)
cell culture - (reply: 1)
cells shrink, detach from each other when changing media - (reply: 3)
Newbie help: Seeding cells to 6-well plates - (reply: 2)
3t3 cell tured black - (reply: 9)
calculating the number of cells to freeze down - (reply: 2)
Fixed cells, sticky cells? - (reply: 2)
What is cotransfection in molecular cell biology? - (reply: 1)
BSA in adipocyte cellculture? Alternatives?? - (reply: 1)
serum deprivation of cell lines - (reply: 1)
serum deprivation of cell lines - (reply: 1)
What to do Post-transfection of cells with siRNA? - (reply: 2)
Problem with HEK stable cell line - (reply: 1)
HEK 293 cell culture - (reply: 3)
HepG2 cells resistant to Trypsinization - Please help!! - (reply: 8)
difficulty in passaging HK-2 cells - (reply: 5)
difficulty in passaging HK-2 cells - (reply: 2)
qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
More cells in an antibiotic plate than in a plate without antibiotic???? - (reply: 2)
what are these cells?? - (reply: 1)
How to choose the right plasmids for certain types of cell? - (reply: 1)
Isolation of rat endothelial cells - (reply: 2)
protocol for making e.coli competent cells under low temperature - (reply: 1)
Looking for cell surface markers. - (reply: 3)
common nuclear marker for T cells - (reply: 1)
How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
Any reliable markers for mouse memoery T cells? - (reply: 1)
Do mouse endothelial cells express the alphaVbeta3 integrin? - (reply: 1)
Cell Culture debris or contamination? - (reply: 4)
primary cell isolation from CNS, how to remove myeline debris - (reply: 1)
Platinum-A cell trasnfection toruble - (reply: 1)
cell culture media refrigeration - (reply: 4)
abnormal epithelial cells in mouse mammary gland - (reply: 1)
cell DNA content of cells grown on different scaffolds - (reply: 2)
Cancer cell starvation - (reply: 1)
Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
Housekeeping gene for COS-1 cells - (reply: 1)
applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
Monocytes die after cell scraping - (reply: 2)
The water pan got dry in the TC incubator. Will my cells be OK? - (reply: 1)
Cell fractionation - (reply: 2)
Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
Dropped cells into liquid nitrogen tank - (reply: 3)
5-FOA media not letting ANY cells grow... help! - (reply: 1)
Small intestinal cell line for transport studies - (reply: 4)
help needed in determining volume of cells to be seeded - (reply: 6)
Dilution, Cell concentration & DNA content - (reply: 3)
competent cells - (reply: 2)
Cell lines & gene of interest - (reply: 1)
Software for selection genes related to concrete cell processes - (reply: 1)
Immunocytochemistry with HEK293 cells - (reply: 1)
How to determine apoptotic cell types in-vivo? - (reply: 1)
Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
data analysis for single cell size and distribution - (reply: 1)
determining optimal cell count for MTT - (reply: 2)
Extracting DNA from FACS sorted cells - (reply: 3)
Storing frozen cells in -80 instead of liquid nitrogen - (reply: 5)
phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
DG44 CHO cells and Methotrexate - (reply: 1)
extract plasmids from transfected eukaryotic cells - (reply: 6)
Trypsin Deactivation - (reply: 4)
Staining Cells in Suspension - (reply: 4)
cell culture confusion - (reply: 1)
a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
chambered cell culture-slide for confocal microscopy - (reply: 3)
Nicotiana tabacum (BY-2) Suspension cells contaminated! Help! - (reply: 1)
urgh...mycoplasma drama..... - (reply: 5)
Dead or Alive... -_- (MCF-7 cells) - (reply: 7)
how to wash cells after fixation for a live binding study - (reply: 1)
Evenly distribute 100ul of cells in a 48/96 well - (reply: 2)
Cell lysis before shearing - (reply: 5)
really low puromycin concentration for selection of cells - (reply: 6)
Single stain control for 293 cell line - (reply: 4)
Is mCherry denatured by Trypsin or RIPA buffer? - (reply: 7)
loading dendritic cell with antigen - (reply: 1)
Cell Lysis and Western Blotting - (reply: 1)
problem with embryo carcinoma cell line , p19 , - (reply: 1)
problem with embryo carcinoma cell line , p19 , - (reply: 1)
Recombinant protein from cell lysate - (reply: 4)
How to test if a compound is entering cells? - (reply: 3)
help with cell seeding - (reply: 2)
SURE or Stbl competent cells for retroviral vector? - (reply: 2)
RNA isolation from frozen cells - (reply: 2)
How do you coat coverslips before seeding cells? - (reply: 3)
Buy.Ask for buying some cell lines and antibodies - (reply: 3)
competentce cell, cloning - (reply: 2)
Flp-recombinase target site integration in 293 T-REx cells - (reply: 1)
Storage of transfected PC12 cells - (reply: 1)
cell adhension - (reply: 1)
Using Methanol For Cell Lysis - (reply: 1)
Adjusting pH of cell culture medium for ELISA - (reply: 1)
Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
Contamination of primary cells with fibroblast. - (reply: 1)
Help With Lysing HEK293 & Fibroblast Cells - (reply: 1)
problem with siRNA transfection of HEK cells - (reply: 5)
Splitting A549 cells - (reply: 9)
NMDA Transfection in 293 Cells - (reply: 1)
freezing adherant cells - (reply: 2)
freezing adherant cells - (reply: 2)
Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
cells stably expressing T7 RNA polymerase - (reply: 1)
Cell Fixation - Experience with cell fixation? (reply: 5)
picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
Is pCL-Eco suitable for packaging viruses that used to infect human cells? - (reply: 1)
Cause of Death for Cell Culture? - (reply: 6)
creat a SY5Y stable cell line - (reply: 1)
selecting drug-resistant colon cancer cells - (reply: 2)
Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
Helper T cell skewing - (reply: 5)
Optimum serum starvation period for NIH, CHO, COS7 cells - (reply: 4)
Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
competent cells stock - (reply: 3)
intracellular cytokine staining - re-stimulation of activated T cells (reply: 2)
DH5alpha cells - (reply: 4)
pheonix cells and &293A cells - (reply: 1)
could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
Protein purification from HEK 293 cells - (reply: 1)
Growing hybridoma cells in medium without serum - (reply: 2)
Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
How many cells in plant embryos ? - seed biology (reply: 1)
cell count - (reply: 8)
question on making chemical and electrocompetent cells - (reply: 2)
COS7 cells giving me problems - (reply: 1)
Where to get 5T series cells? - (reply: 1)
cell number for colony formation - HEK293, NIH3T3 (reply: 1)
Electroporation/electrotransfection mammalian cells - (reply: 7)
Cell debris here, there, everywhere!? - (reply: 2)
Cell debris here, there, everywhere!? - (reply: 1)
Mini prep of frozen bacteria cells - (reply: 2)
Help! Turned on UV light. Did I kill my cells? - (reply: 2)
growing competent cells - (reply: 1)
Freezing density for NIH 3T3 cells - (reply: 3)
Stable Cell Line generation - (reply: 3)
making more competent cells - (reply: 2)
CellTracker dye in necrotic cells? - (reply: 1)
The case of the punky Sf9 cells - (reply: 2)
sticky perm cells (tween 20)- can I prevent it? - (reply: 2)
Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
collecting cells HT1080 - (reply: 4)
Expired media - How long after the expiration data is cell culture media good? (reply: 2)
Help: problem with MDCK cells - (reply: 2)
bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
Oh my cell culture problems never cease!!! - (reply: 4)
WEHI-3BD cell culturing - Advice on culturing. (reply: 5)
cell homogenization - (reply: 13)
cell count - (reply: 3)
cell lysis - confocal slides (reply: 4)
HeLa cell anatomy question - (reply: 3)
Need cell lines wich express separately TLR 2, 4 and 5 - (reply: 1)
MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
THP-1 cell line - (reply: 3)
CaMKII alpha promoter and cell tranfection - (reply: 1)
Adaptation of mammalian cells to serum free mode - (reply: 2)
Live cell Imaging medium - (reply: 1)
Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
MDA-MB-231 cell transfection - (reply: 4)
Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
Break Cell Clumps - prepare single cells for injections (reply: 1)
Stem Cells Sphere - stem cells (reply: 2)
Request for cell lines? - (reply: 1)
Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
MTT problems with Huh7 cell line, plzzzzzzz help - MTT color problem (reply: 5)
Calculating cell doubling time - (reply: 1)
SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
Infected cells that are dying after puromycin selection - (reply: 1)
contamination issue in cell culture - (reply: 1)
Why do cells need to be washed in PBS before biotinylation? - (reply: 3)
AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD (reply: 2)
Purification of recombinant proteins from insect cells - (reply: 2)
Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
Growth curve for PC3 cell line - (reply: 1)
Agrobacterium EHA101 competent cells - (reply: 1)
THP-1 cell line - (reply: 2)
Effective DCFDA Protocol for Non-Adherent THP-1 Cells? - (reply: 1)
Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
best freezing method for electrocompetent cells - (reply: 9)
cell seeding calculation - (reply: 8)
Improved Stem Cell Proliferation - Would interested in improving proliferation of adult stem cells? (reply: 5)
Can one use Magnesium chloride for competent cell prep. - (reply: 1)
Yeast contamination in cell culture - (reply: 8)
Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
Remove cells from dish and fix them in slides - (reply: 3)
Help- lysing cells with triton x-100 - (reply: 1)
E.coli cells lyse in minimal media - (reply: 3)
How stable are suspension cells in room temperature? - (reply: 2)
Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
Transfection in insect cell lines?? - (reply: 1)
PCR genomic DNA using cell lysate - (reply: 1)
HELA Cells Capable of producing whole, infective HPV particles? - (reply: 3)
Distilled water for Electrocompetent Cell Preparation - (reply: 6)
Should I fix cells digested from tissues? - (reply: 4)
STR cell line identification - looking for cell line identification method (reply: 1)
Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
Rat Dental Pulp Stem Cell Culture - (reply: 1)
NK cell analysis using CD16PE and CD56PE - (reply: 2)
My neuroblastoma are floating!! please help - Both SK-NSH and SH-SY5Y are adherent cells, but are only floating now (reply: 3)
cell suspencion and medium - (reply: 5)
One Shot TOP10 Cells - (reply: 6)
trypan blue for chlorella - (reply: 1)
Help on BmN4 cell culture - How to maintain BmN4 cells? (reply: 1)
Compare ChIP to cell lysate - (reply: 1)
Compare ChIP to cell lysate - (reply: 1)
Testing cytoxixity on healthy human cells - (reply: 2)
what cell line to use - to demonstrate ubiquitination (reply: 1)
GFP cells intracellular staining - (reply: 1)
Xenograph - how to maintain cell viability? - (reply: 1)
Western blot of apoptotic cells - (reply: 2)
cell sycle and proliferation - (reply: 3)
help with cell dilution - (reply: 2)
Difficult Cell Proliferation Experiment - (reply: 2)
Cell dilution cloning - Cells cloning (reply: 2)
Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
HTC116 cells detaching !!!! - (reply: 2)
Revival of kasumi-1 cells - (reply: 3)
HEK 293 Trypsin vs. only EDTA - (reply: 2)
Growth conditions for E. coli DB3.1 cells - (reply: 1)
Mycoplasma cleanup time... - (reply: 10)
weird & curious problem with CHO cell culture - (reply: 3)
HaCat do not grow after using cell scraper - (reply: 3)
MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
Cell Lysates from a Western Blog - (reply: 2)
Loss of YFP signal in transduced T cells - (reply: 7)
Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
Freezing cell pellets and minimizing lysis - (reply: 2)
Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells? (reply: 6)
Total Cell protein - (reply: 1)
Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
Mycoplasma - (reply: 1)
cell culture media - glutamine! - (reply: 2)
How many cells do you have in a confluent T 75 flask? - I have 1/2 million (reply: 1)
how to make antigen by trypsin and papain digetion - plz teel me hoe to make antigen by trypsin & papain digetion (reply: 1)
can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
Premature cell lysis - (reply: 2)
urgent!!! weird Growth in cell line - (reply: 8)
refrozen cell pellet - (reply: 1)
Drug vehicle giving toxicity in cell culture - With increse in vehicle..... toxicity decrese (reply: 4)
contamination in cell line...... - (reply: 4)
Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 6)
Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
MTT with cells growhing in suspension, not adherent - (reply: 3)
Cell ELISA - No differences between cells expressing and not, the receptor (reply: 2)
growth curve for attached cells - (reply: 1)
cells dont attach hard enough to 96 well - (reply: 3)
CTLL-2 culture - preventing cell clumping - (reply: 2)
Yet another contamination question - SF9 cells (reply: 5)
Problems with transfection in human cells - (reply: 3)
HepG2 cells culture conditions - (reply: 2)
Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
T cell proliferation problem - (reply: 2)
How to Handle Cells Shipped Internationally in Dry Ice? - (reply: 2)
Differences between cells in different species - (reply: 5)
Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
isotype control for GFP expressing cells? - (reply: 2)
Trypsin for J774A Macrophages? - (reply: 2)
Trouble with growth of transformed cells - (reply: 4)
nuclear extraction of blood cells - (reply: 2)
Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
ELISA with undiluted cell lysate samples - (reply: 1)
How can you expand cells if you have one dish of them? - (reply: 1)
Androgen independent LNCaP cells - (reply: 2)
mycoplasma detection kit - (reply: 1)
What is the proper way to infect cell culture with virus? - (reply: 5)
Micro-RNA profiling for differentiating cells - (reply: 1)
Growing insect cells with other cell lines - (reply: 2)
Candida albicans cells lysis - method (reply: 1)
Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
Advise for Whole Cell Stain - hESC-differentiated Trophoblast (reply: 1)
293 T cells - (reply: 3)
Mouse Bone Marrow Isolation - How to increase yield of BM-derived cells? (reply: 1)
BL-21 Gold (DE3) cells and further strains - (reply: 2)
Cell Cycle Analysis flow cytometry - How to measure desynchronisation rate of cells (reply: 1)
Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
Fixing cells before staining of surface markers - (reply: 1)
Black dots in cell culture - (reply: 7)
cell counting - (reply: 5)
Optimization for cell viability assay (MTT assay) - (reply: 1)
Anyone thought about reprogram fibroblast to liver cells? - (reply: 2)
cell confluence in cell cultures - myoblast c2c12 in particular (reply: 2)
cell line adherence - cells adhering to flask very tightly after transfectiono (reply: 1)
Cell labelling - How can I label 2 cell populations? (no transgenes) (reply: 1)
How long does it take for cells to reattach? - From balls to pancakes. (reply: 3)
Dot plot cell cycle analysis-strange dots - (reply: 4)
TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
help Splashed Raji cells on skin/face/hand - (reply: 2)
Help! ES Lenti infection efficiency=nightmare - trouble getting over 40 % cells infected (reply: 8)
Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
CO2 level for cell culture - (reply: 6)
sphere and suspension culture subculturing - (reply: 1)
suspension cells for confocal - (reply: 1)
RNAi is somatic and germline cells - (reply: 3)
Luciferase and cell responsiveness - (reply: 3)
adult cell culture - (reply: 1)
protein overexpression in HeLa cells - (reply: 1)
Insulin supplements to cell culture media - (reply: 1)
After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
topic and cell culture - (reply: 2)
Running a western with cell media in gels? - (reply: 11)
Starting cells for making one's own competent cells - (reply: 7)
cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
Persistent stable cell line (Bos23) Contamination... - (reply: 2)
Hematopoietic cells separation - How to get high purity and viability hematopoietic cell populations? (reply: 2)
Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
min6 cells immunofourescence staining - staining and culture problem (reply: 4)
2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
rat ES cells - (reply: 2)
Mycoplasma contamination - (reply: 1)
Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
establishment of primary cell line from biopsy - (reply: 2)
Cell culture fasting - (reply: 1)
mutated HeLa cells? - from cycloheximide? (reply: 1)
Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
qPCR for transformed cells - (reply: 1)
solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
descrepancy between total cell lysate and cell fractionation - (reply: 1)
Disruption of cells using mild detergent - (reply: 2)
Eruption of mammalian cells using detergent - (reply: 1)
lenti virus transfected cell dies... - (reply: 4)
Extremely Weird Issue - CHO Cell Culture (reply: 2)
Cell Die.. Need help - (reply: 1)
Cell scraping ruining my life - (reply: 6)
How much trypsin/Cause of death - (reply: 3)
Death of cells in the control. - (reply: 1)
cell culture clarification - I need help please (reply: 2)
Serum concentration in treatment of cells for MTT assay - (reply: 1)
Minimum number of cells needed for cytometry? - (reply: 3)
Beta-Lactam in CHO Cells - (reply: 1)
problems with 293T cells after medium removal!!! - (reply: 2)
B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
Propagating ccdB survival cells - (reply: 5)
DOubt - Competent cell (reply: 2)
Differentiation of CD11b cells into macrophages.Help please - (reply: 1)
Please Help!! - Mycoplasma (reply: 5)
Primary keratinocyte isolation- cell loss - (reply: 1)
cells attach but don't flatten out - MDA-231 (reply: 8)
Problem Generating Stable HeLa Cells - (reply: 1)
Retroviral transduction of T cells - (reply: 4)
Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
Multiple nuclei?? - cell morphology - FaDu (reply: 8)
Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
how to compensate the cell death during transfection by electroporation - (reply: 2)
H-ras transformed RK3E/tv-a cells - (reply: 1)
HEK001 Cell Line and Papillomavirus - Does HEK001 cell line produce a functional papillomavirus (reply: 4)
2 questions: myeloma cell lines and mice MABs - (reply: 5)
I'm getting fried-egg looking cells... What should I do?? - (reply: 8)
Empty bubbles in the 3T3-L1 cells - (reply: 3)
Exposing Cell Lines to Drugs Metabolized by Liver Microsomes - (reply: 1)
UV safety in cell culture hood - (reply: 3)
Where can I find info on the physical appearance of TB cell wall structures? - I am an artist wanting to depict the cell wall with absolute accuracy. (reply: 7)
intracelluar staining without killing cells - (reply: 3)
Info about A7r5 cell culture - (reply: 1)
Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
Cell lysates - Protein precipitation? - (reply: 3)
Glucose in epithelial cell - (reply: 1)
cell lysate for pulldown - (reply: 3)
cell culture media - (reply: 3)
Competent cell storage for long term use - (reply: 2)
My RIPA cell lysis buffer - Western blot (reply: 3)
Cell Uptake Study - (reply: 1)
what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
aspects of RAW264.7 cells - (reply: 5)
anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
Hoechst Stain does it stain dead cells - (reply: 3)
Mycoplasma detection - (reply: 2)
Help for cell preparation for FACS... - (reply: 1)
Cell Lysis Buffer - (reply: 6)
HeLa cells and HPV - (reply: 2)
Resolving cell growth problems using growth additives - way to resolve urgent cell growth problems (reply: 3)
Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
problem with MACS cell isolation - (reply: 5)
cell culture split ratios. - (reply: 2)
MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
detection of mycoplasma with hoechst staining - (reply: 1)
blood red cells - (reply: 1)
Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
NFKb signalling in cell line ??? - (reply: 2)
kit fpr cell membrane preparation? - (reply: 2)
Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
How to find NFKb signalling is active in a cell line??? - (reply: 2)
Fixing YFP cells - (reply: 2)
MTT Assay: cell proliferation or cell viability??? - (reply: 4)
Rescue RAW cell culture - RAW cells past confluent point... can they be rescued? (reply: 4)
cell cycle arrest by colcemid - (reply: 2)
is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
CD19 Expression at various stages of plasma cell differentation - (reply: 1)
PROLIFERATION ASSAY IN MICROTITER PLATES WITHOUT A SEMIAUTOMATIC CELL HARVESTER - (reply: 1)
THP cells? what does THP stands for?????????? - (reply: 1)
Immunioprecipitation from Jurkat cells?? - (reply: 1)
Weird effect of siRNA on protein in cells - (reply: 3)
why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
EDTA addition to trypsin solution (1x) - (reply: 1)
Apoptosis induction - for vero and mcf-7 cells (reply: 5)
Need pH 6 Cell culture media - (reply: 1)
Are my cells undergoing epigenetic changes? - (reply: 2)
establishment of stable cell line using hek293t - (reply: 2)
Cell pellet frozen -80 then -20 - (reply: 4)
total cell protein vs membrane protein - (reply: 4)
tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
Fixing the cells for FACS - Paraformaldehyde or Formaldehyde (reply: 2)
Problem with control samples in cell IC - (reply: 3)
Recommendation for cell cycle analysis software needed - (reply: 1)
Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
Cell culture contamination question - what the hell are these things?? - (reply: 3)
'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
Immunofluorescence using dead cells? - (reply: 1)
floating THP-1 cells - medium change advice - (reply: 2)
my cells love infection, any thoughts on antibiotics? - (reply: 3)
Culture Media for stem cells - (reply: 1)
Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
Anyone heard of cells spontaneously fluorescing red?? - (reply: 4)
Freezing cells - How long can I keep cells at -80C? (reply: 6)
Help regarding cell line - (reply: 1)
organizing cell lines - (reply: 1)
Cell plating density - (reply: 3)
Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
MDA-MB-231 cells question - (reply: 3)
thawed cells wont adhere - pc3 and LNcaP (reply: 2)
DiFi Cell line (colon carcinome) - Where can I get it???? (reply: 1)
HaCaT cells - (reply: 2)
lncap and MCF-7become ethanol sensitive? - are lncap cells and MCF-7 cells ethanol-sensitive? (reply: 2)
Dying endometrial stromal cells - (reply: 1)
Help me - Are my cells infected with something or could this be unsusual morphol - (reply: 5)
how to dissociate cells for cell migration assay - (reply: 3)
soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
RNA extraction - how many cells do I need at least?? (reply: 4)
RNA extraction protocol for small amounts of cells - (reply: 2)
about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
strange morphology of sp2/0 cells after fusion!!! Please help! - morphology switch from round to triangular!!!! (reply: 2)
fabrablast primery cell line problem - how to detach cell well (reply: 1)
what solvent is better compatible for most cell lines? - DMSO vs methanol (reply: 3)
Cell growth rate & O2 consumption - (reply: 2)
I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
Cells stick to dish in Embryoid Bodies - (reply: 1)
Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
how to plate cells precisely - (reply: 1)
RNA extraction from stained cells - (reply: 2)
Methods to assess cell viability - (reply: 1)
suspension cell transient transfection - (reply: 9)
Adherent cells washing off in 96 well -MTT - (reply: 4)
Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
plasmids - plasmids for stable cell line construction (reply: 2)
abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
average total protein content per 293t cell - (reply: 2)
T cells preparation for FACS - (reply: 1)
counting cells - (reply: 3)
Cell OD and spec problems - (reply: 1)
cell confluency & effect on cells...?? - (reply: 4)
Pipet trypsin tips - (reply: 3)
Fixing cells for flow cytometry - alternative to 1% paraformaldehyde (reply: 3)
mycoplasma false positive - Luminescence false positives for myco (reply: 2)
Washing Red Blood Cells - Tips on washing red blood cells (reply: 7)
MEF Cell marker - (reply: 1)
(CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
Change of colour in cell culture media...? - (reply: 2)
cell count - how to calculate (reply: 2)
generating cell line knockouts - (reply: 3)
Cancer Stem Cells?? - (reply: 10)
Some kind of contamination? - mouse transduced cell line (reply: 7)
Cell stimulation of transcription - (reply: 5)
Mouse blood density/osmolarity of blood cells for separation - (reply: 5)
suitable cell lysis buffer - (reply: 1)
Toluidine blue staining for cell culture - (reply: 1)
cell culture - 3D cell culture (reply: 1)
Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
MEF culture contamination? - Contaminated cell culture? (reply: 2)
very simple question about hemocytometer cell counting - (reply: 1)
nucleic acid extraction from silenced cells - (reply: 6)
direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
In Vitro Drug Exposure Cytotoxicity Testing and Cell Confluency - (reply: 8)
Dendritic cells - Protocol of stimulation of dendritic cells (reply: 3)
giant cells in HEK293 cell line - (reply: 10)
Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
Plant cell transfection - (reply: 1)
stable cell line - selection after retroviral infection (reply: 1)
Storing cell lines long-term at -70C - (reply: 4)
Commercially available primary mouse endothelial cell line? - (reply: 1)
Frozen cells in -80 - (reply: 1)
Plant or Fungal Cell Dissociation Technique? - (reply: 3)
how can i bring cells onto a slide? - ...for microscopical observation! (reply: 7)
cell density to be seeded? - cytotoxicity studies (reply: 1)
Lysis of the cells - (reply: 1)
Cells do not lyse in SDS buffer - (reply: 1)
Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
lysis buffer for lymphoblast cells - (reply: 1)
problem with cell cycle analysis - (reply: 3)
THP-1 Cells differentiation - THP-1 cells differentiated using IFN-gamma/LPS (reply: 6)
Single cell suspension from solid tumor - (reply: 3)
T-Cell receptor - Is it a single type on a T cell or multiple types? (reply: 1)
cells weak adhesion - detachment (reply: 2)
how we count the passages in cell cultures? - (reply: 5)
resolving cell growth problems - (reply: 2)
clumping of cells - (reply: 7)
stability of cell suspension - (reply: 1)
cell adhesion in serum free condition - (reply: 1)
effects of cell confluency - (reply: 1)
Which trypsin EDTA to use? - (reply: 1)
Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
polarized cells - (reply: 4)
PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
cell adhesion assay - using collagen (reply: 1)
Problem clariflying/filtering insect cell lysate - (reply: 2)
cell culture urgent!! - (reply: 2)
Moving S2 cells from one plate to another - (reply: 1)
Cell culture medium instead of Nucleofector solution?? - (reply: 3)
Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
mononuclear cells medium - I am stuck with the concentration of 2-mercaptoethanol (reply: 1)
Accidental thawing of frozen cells - (reply: 3)
how to start with a new cell line ? - how to start with a new cell line ? (reply: 1)
HaCaT cells die after trypsinization - (reply: 1)
Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
GFP and Luciferase Cell Line - (reply: 2)
Transfecting primary cells with plasmid - (reply: 1)
transfecting and selecting EL4 cells - (reply: 1)
Induce Osteoblast lineage from C2C12 myoblast cells - (reply: 1)
Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
Transfection into eukaryotic cells - (reply: 1)
Fixing cells with PFA - (reply: 1)
A549 cells not doubling - has anyone been through this with other cell lines? (reply: 4)
Contamination in SH-SY5Y cultures, mycoplasma? - (reply: 2)
Immunofluorescence in HEK cells issues - (reply: 1)
cell sorting - losing cells? - (reply: 3)
What if my expressed protein is toxic for the cells... - (reply: 1)
competent cells - difference between electroporation and chemical comp. cells (reply: 1)
Possible contamination of leukemic cell culture - debris, growth inhibition, cell aggregates (reply: 3)
Cell pellet preservation - Advice on how best to maintain cells post-centrifugation (reply: 1)
human cancer cell line splashed into my eyes - (reply: 3)
Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
BL21(DE3)LysS competent cells - (reply: 1)
ATP turnover in neuron - ATP hydrolysis rates in neuronal cells (reply: 2)
Adhering CHO cell culture - cells don't adhere! (reply: 2)
do e.coli cells get ruptured during high spin - (reply: 1)
Please Help with COS-1 cells^^ - (reply: 1)
antibiotics for preventation of mycoplasma contamination - (reply: 4)
IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
cell counting - to get an impression when my cells are in the log phase (reply: 1)
Whole cell lysis of fibroblasts - (reply: 1)
Whole cell lysis of fibroblasts - (reply: 1)
test of cytotoxic effect of compounds - why cells are pretreated with actinomycin C ?? (reply: 1)
Proliferation Assay - Should cells be in the logarithmic phase ? (reply: 3)
cell density and growth - (reply: 5)
Laser capture microdissection - capturing single cells (reply: 2)
when cells have to be synchronized?? - (reply: 2)
cells in RNAlater - the title say`s it all :) (reply: 4)
Cytotoxicity assay for adherent cells exposed to particles - (reply: 1)
Murine Cell Lines - (reply: 2)
Cell Proliferation: To adhere or not to adhere (before treatment), that is the q - (reply: 2)
why are some cell lines more popular than others?? - (reply: 4)
NK cell staining - (reply: 3)
cell culture media - with or w/o L-glutamine? (reply: 2)
Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
pro-apoptotic effect of compounds on cancer cell lines - which cell line to test the non-toxic effect? (reply: 3)
Expiry of Trypan blue - (reply: 3)
Question in Generating double stable cell line - (reply: 3)
activity of sex-chromosome in somatic cells - (reply: 5)
CaCl2 for making competent cells - (reply: 3)
Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
ICC Cell Adherence Issue - (reply: 1)
gentamycin resistance in human cencer cell culture - please suggest the alternative antibiotic (reply: 2)
siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
How to normalize my samples before I transfect my cells - (reply: 6)
Isolation of intestinal epithelial cells - Isolation of intestinal epithelial cells (reply: 2)
Number of proteins expressed in cell - (reply: 2)
Do HEK 293 cells contain functional E1A/E1B? - (reply: 2)
Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
Calcium phosphate method vs lipofectamine - troublemaker neuroblastoma cells (reply: 7)
Which cell lines are best for transient transgene expression? - (reply: 3)
Alzheimer's cell model - (reply: 7)
Partial cell subculture? - (reply: 2)
No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
Whole Kidney primary cell culture - (reply: 1)
dh5 alpha competent cells? - (reply: 2)
which is the best lysis buffer for whole cell extracts? - (reply: 1)
Please help me to understand- cell counting - (reply: 1)
Stimulating hepatocytes with IL-1beta - (reply: 1)
Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
Cell count - Please help - (reply: 11)
What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
Cell Count - (reply: 1)
Generating stable cell line - (reply: 2)
Harvesting cells off of collagen gels - (reply: 1)
cells can't be lysed after crosslinking - (reply: 4)
How many cells to seed? - simple question :) (reply: 5)
Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
mouse macrophage cell line - (reply: 5)
Problem clarifying lysate from Hi5 insect cells - (reply: 1)
cell line information - require (reply: 1)
Urgent problem - Media change = cells die in 48h (reply: 3)
recombineering for ES cell targeting - retrieval problems (reply: 1)
How often do you thaw new cells? - passage number or time? (reply: 7)
Does high speed centrifugation destroy viable cells? - (reply: 2)
Cell culture - (reply: 7)
Problems when plating neuroblastoma cells - (reply: 3)
Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
Cell Doubling Time - (reply: 3)
Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
synchronized cells or non-synchronized cells - (reply: 3)
pre-prepararation of competent cells - (reply: 3)
ROS accumulation - ROS reactive oxygen species accumulation in cells (reply: 3)
Bacterial invasion assay with human cells - (reply: 2)
Measuring gene expression across cell lines - (reply: 2)
trypan blue without hemocytometer? - (reply: 2)
How to make an isotonic solution for h9c2 cells - (reply: 2)
migration < invasion??? - More cells migrating through matrigel than through control inserts (reply: 1)
Zeocin of G418. Which is the better selection agent for CHO cells? - (reply: 1)
Immunofluorescence with transfected cells - (reply: 3)
Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
Contamination in cell cultures - (reply: 9)
DMSO killing more cells at lower concentrations - (reply: 1)
frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
lysing cells on ice - (reply: 2)
Fibroblast cell culture - (reply: 1)
Cardiac stem cells - (reply: 3)
How to visualize tumor cells in mice with out sacrificing the animals - (reply: 3)
cell count and dilution - (reply: 1)
z competent cells - (reply: 2)
immunofluorescence staining of sperm cells - (reply: 2)
Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
colony formation assay - how many cells to seed (reply: 2)
Primaria/gelatin plates and gravity-defying fixed cells - (reply: 2)
Contaminations in cells cultured in suspension - (reply: 2)
suspension cell transient transfection - (reply: 2)
What is this ? - Cell culture contaminant (reply: 2)
cell cryopreservation after siRNA transfection - (reply: 1)
Cell Digestion Protocol - (reply: 1)
spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
cell growth - (reply: 4)
primary cells could not attch to regular polystyrene flasks but immortalised cel - does anybody know what could be happening (reply: 1)
How to culture cells after sorting? - (reply: 3)
How to kill nerve cells in a living hydrozoans? - Trying to procude partial/complete nerveless jellyfish (reply: 3)
lifespan of primary cells in vitro - how long can different cell types survive (reply: 3)
measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
T cell differentiation protocols - Th1/Th2/Treg/Th17 (reply: 4)
Mitochondria extraction from human liver cells - (reply: 1)
lipofectamine and PEI transfection of hela cells - (reply: 2)
What can I do whit my Cancer Stem Cells?(I know, its a big question..) - (reply: 7)
Wash L929 Cells with DPBS (with or w/out CA MG?) - DPBS with or W/out CA,MG? (reply: 2)
Especially tenacious cells -- what to do? - (reply: 9)
MDCK cell vascuolation - I got my MDCK cell growing in the T-75 flask (reply: 5)
Culturing HeLa Cells - (reply: 2)
expression of His-tagged protein in insect cells - (reply: 2)
How to isolate vascular smooth muscle cell? - (reply: 1)
Whole cell lysate including nucelar proteins? - (reply: 3)
Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
culturing MCF-7 cellls - clumping and maintaining healthy cells (reply: 3)
Cell culture media - require information on media (reply: 3)
how to measure ROS in cell wiht H2DCFH - (reply: 4)
VEGF in cell nucleus - (reply: 2)
cell treatment rules - (reply: 4)
method to seed cells in hyrogels - cell seed in hydrogels (reply: 1)
transfecting human mesynchemal stem cells - (reply: 5)
cell schock - (reply: 2)
Flow cytometry of RFP and CFP expressing yeast cells - (reply: 1)
Can you store cell pellets for future nuclear extraction? - (reply: 3)
using cell lysate to run native gel? - (reply: 1)
Cell Suspension from Frozen Tissue? - (reply: 6)
looking for stromal cell marker - (reply: 1)
Fixing for Cell Migraton - (reply: 1)
'Black dots' in cell culture - (reply: 2)
ChIP in WT and KO cells - how to analyze - (reply: 1)
Mouse T cell line - (reply: 2)
Making competent cell from freeze-thawed stock - (reply: 2)
Invasion assay using matrigel- cell clumps - (reply: 1)
producing cell lines - why viruses ?? (reply: 4)
What color should the cell pellets be after sonication and centrifugation? - (reply: 1)
Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
Mycoplasma Question - (reply: 2)
Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
How to detach an antibody from cell surface - (reply: 4)
Water for electrocompetent cells - (reply: 2)
Gp 293 Luc cells - (reply: 1)
how to check cell viability - (reply: 1)
Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
cell fixation - (reply: 1)
cell detachment in IHC - can I incubate cells with hoechst, heparin and PFA at the same time? (reply: 1)
cell immobilization for confocal - (reply: 1)
problem with 293T cell co-transfection - (reply: 5)
Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
Cell Death after formaldehyde treatment - Immunofluorescence (reply: 5)
Cell Densitometry - (reply: 1)
My cells died after changing media... - (reply: 6)
Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
H9C2 cells - trouble when culturing H9C2 cells (reply: 4)
Contamination of cell ?? - (reply: 2)
Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
Passaging Cells - (reply: 4)
Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
Mysterious cell death - need Hela Help! - (reply: 1)
NSC-34 Cells sloughing off at media change - (reply: 4)
Chosing the right competent cells - (reply: 1)
Cell proliferation assay for LNCaP cells - (reply: 3)
liquid nitrogen in cell culture - (reply: 2)
differential cell counting in mixed cultures - (reply: 1)
Questions about animal cell cultue Please answer - (reply: 1)
immunofluorescence for HUVEC - how to do immunofluorescence for cancer cells (reply: 3)
membrane type of cell culture filtration - (reply: 3)
Critical column of media above cells for access of oxygen - (reply: 1)
EDTA bacterial cell lysate - (reply: 2)
Is MTS Assay suitable for Adherent cells? - Confused about MTT Assay and MTS assay.. (reply: 1)
membrane damaged = cell death? - (reply: 2)
293 cells help! - (reply: 1)
stable cell line - (reply: 2)
Color of cryopreserved cell line - (reply: 1)
Established (tumor) cell line - or not...? - (reply: 3)
Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 2)
proliferation assay for small cell population - (reply: 2)
Cell fixation with glutaraldehyde - (reply: 2)
Yeast contamination in cell culture - hood to blame? - (reply: 6)
Comparing cell # or DNA content - of pelleted cells (reply: 1)
bicarbonate in cell culture: powder v. liquid? - (reply: 3)
cell adhesion assay - collagen (reply: 1)
To break cell clumps - suspension culture related (reply: 4)
Problems for MTT in SH-SY5Y cell line - (reply: 1)
cell stainer size - (reply: 3)
FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
Difficulties with the transfection of cells CHO DG44 - (reply: 9)
Low concentration of FBS inhibiting trypsin? - (reply: 1)
contamination in establishing stable cell lines - (reply: 1)
How much cell medium for 1 week of culturing? - (reply: 1)
p53 +/- matched-pair tumor cell lines? - (reply: 2)
Calcium Flux Primary Mouse T Cells (Fluo-4) - (reply: 1)
Tranfection cell density and overgrowth - (reply: 3)
shRNA selection in suspension cell culture - (reply: 6)
trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
Ethanol/methanol/dmso for cell culture - (reply: 1)
stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
Rapid Cell Count (in vivo) - (reply: 2)
cell migration assay (corning transwell) - (reply: 2)
cell culture in 2 stack flask - (reply: 1)
Invitogen cell countess - (reply: 5)
Hypoxia Treatment For Cell Culture - (reply: 4)
Picking strain for e coli competent cells - (reply: 6)
cell death - can anybody tell me what process this looks like (reply: 3)
Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
Marker do discriminate cell cycle phases - G2 phase / Mitosis (reply: 3)
hematopoietic cell lines - (reply: 3)
Cos7 Cells to produce retrovirus - (reply: 2)
how to make nonadhensive cells attach to coverslips without affecting cellular s - (reply: 1)
Constructing puromycin kill curve - How to count the number of adherent cells without detaching them? (reply: 3)
stable cell line generation - (reply: 5)
histological staining for plant cell wall components - (reply: 1)
My adherent cells are detaching - (reply: 2)
Cell culture media - DMEM and DMEM/F-12 (reply: 1)
Cell Cycle analysis of proliferating lymphocytes - (reply: 1)
Problems with HEK293 cells in 96 well plate format - 293 cells do not adhere well to the plate (reply: 1)
Cell Synchronization - (reply: 1)
Slow cell culture growth? - (reply: 3)
Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
cell lysis buffer - (reply: 1)
cell aging - cell culture (reply: 2)
cell concentrations - (reply: 4)
Floating cells...pls help - (reply: 6)
Cell migration assay with 3 cell types - (reply: 1)
Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
How many B cells do I need to get a 1mg of protien - (reply: 2)
Do histology staningings work in cell culture? - (reply: 2)
Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
Trypsin Activity Assay - No Change (reply: 4)
T cell transduction - (reply: 3)
compound that block DNA replication in human cell - (reply: 1)
Tranfection of HL 60 cells - (reply: 1)
mouse liver cell line? - (reply: 1)
Failed Transformation of Tuner (DE3) cells - (reply: 1)
Cells suddenly died after changing the medium... why? - (reply: 4)
type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
Food colouring agar plates - Do you think it will affect cells? (reply: 11)
CHO Cell contamination - (reply: 6)
MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 2)
Neuronal precursor cells - (reply: 1)
Sticky cells - (reply: 3)
Having problem establishing stable cell line...... - (reply: 5)
Transferring mamallian cell colonies for selection - (reply: 2)
Antibody titration and Cell number - (reply: 1)
Cell Invasion Assay - possible to analyze with flow cytometry? (reply: 5)
Cell culture contamination - "outsider I.D." (reply: 2)
Alternative media for PC-12 cells - (reply: 2)
viability of adherent cells - (reply: 3)
How long can viably frozen cells stay good in -80 degree? - (reply: 5)
Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
HeLa cells and BCL 2 expression - (reply: 1)
B104 cells - (reply: 2)
SH-SYS5 cell culture for dummies... - (reply: 4)
collagen staining in cell culture - (reply: 3)
Hematopoietic lineage depletion kit - Hematopoietic stem cell enrichment kits (reply: 4)
changing cell shape - (reply: 2)
how to change cell shape? - (reply: 3)
About T-antigen and tumor cell lines - (reply: 2)
Sub-culture of N2a cells - (reply: 1)
Haematopoietic stem cells - (reply: 5)
CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
proteomics - minimum cell number (reply: 1)
Standard Curve for OD Turbidity vs Cell density - (reply: 6)
Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
Plasmids for protein expression in mammalian cells - (reply: 2)
cell lysis after induction - (reply: 1)
competent cell preparation? - (reply: 1)
One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
Fresh Thaw Jurkat -what is the procedure in spinning out dead cells using lympho - (reply: 1)
Dendritic cell infection - (reply: 2)
Competent Cells - (reply: 5)
looking for bovine skeletal muscle cells - (reply: 5)
my cell line doesnt change the colour of the media - even when cells are 100 % confluent (reply: 5)
Dead Dendritic cells ... please help - (reply: 1)
Cell concentration Question - (reply: 5)
cho cell line adoptation to serum free medium - (reply: 1)
Problems with viability of Jurkat's cells - (reply: 6)
THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells (reply: 1)
24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
Culturing U2OS cells - (reply: 1)
staph cell count - (reply: 5)
What kind of speed for HepG2 cells. - (reply: 2)
Serum free culture media 293T cells? - (reply: 1)
Embryoid bodies - How to differentiate mouse ES cells quickly and efficiently (reply: 8)
cell count - is it right? (reply: 3)
Haemocytometer cell count - Coverslips (reply: 4)
Growing two cell types in the same flask?? - (reply: 1)
cytokine receptor expression on monocytes - and U937 cells (reply: 1)
fixing bacterial cells in ELISA - (reply: 1)
Cell isolation - (reply: 1)
trouble to grow cells on top of coverslip - (reply: 6)
Immortalized murine hepatocytes - (reply: 1)
about measuring luciferase activity in living cells - (reply: 8)
Culture conditions for cell treatments - (reply: 4)
preadipocyte 3T3-L1 cells question? - (reply: 1)
SH-SY5Y cell culture - Suspended cells (reply: 4)
Magnetic-activated cell sorting ? - (reply: 4)
mRNA level in transgene cell line or knocking down cell line - (reply: 3)
How to increase IF signal in acetone/methanol fixed cells? - (reply: 5)
Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
sheep red blood cell rosetting protocol - (reply: 1)
Tranformation cell harvesting - (reply: 5)
self-renewal Vs proliferation - stem cells (reply: 3)
Plyse cell and DH5a - (reply: 1)
Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
INS-1 cell line - (reply: 1)
Central cell seeding - (reply: 4)
please help with Freezing primary cells - (reply: 1)
COMPETENT CELL PREPARATION - (reply: 6)
MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
suggest cells expressing low levels of adhesion molecules - (reply: 1)
Cell Culture and Pipette Tips - (reply: 4)
Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 4)
Cell counting issues - the ring of death on my flasks (reply: 5)
Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
RNA Isolation - RNA isolation from human cell lines (reply: 2)
Cell Fixation - methanol fixation of cells (reply: 3)
How to retrieve my human blood immune cells from liquid nitrogen without killing - (reply: 3)
stem cells and EB (Embryoid bodies) - (reply: 1)
Immunohistchemistry of red blood cells - (reply: 1)
cell cryosection - (reply: 5)
Shifting of entire cell cycle to the left - (reply: 3)
freezing suspended cells - (reply: 7)
Cell Brakeage...how will I know - I need the mitochondria. (reply: 1)
How many cells... - ...gives you how much RNA? (reply: 2)
Cell culture - Culture medium (reply: 1)
Anybody ever use whole blood plasma serum stimulate cells? - (reply: 2)
How can I separate my cell line from feeder cells - (reply: 3)
ChIP-seq: Minimum number of cells - (reply: 3)
CHO or VERO? - Cell culture (reply: 2)
Media for squamous cells - (reply: 3)
how to trace single cell movement throughout gastrulation of zebrafish - (reply: 4)
Oxygen diffusion through cell culture medium - (reply: 2)
Request for Mono Mac-1 cell line - (reply: 1)
help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
How much sonication power needed? - protein extraction from cells (reply: 2)
protein extraction - from cell line in T25 flask (reply: 5)
Getting cells from flasks - (reply: 5)
transformation efficiency decreased - storage conditions of competent cells (reply: 7)
Fixation of immune cells - "how to" protocol needed (reply: 2)
cell cycle analysis by FACS - (reply: 2)
Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
Trypsin Expression and Purification - (reply: 3)
cell cycle analysis PI staining shift - (reply: 1)
Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
Problem with stable overexpression cell lines - (reply: 3)
seeking help in stable cell line generation - (reply: 3)
SDS, Cell numbers, dilution - (reply: 18)
Help with Hep G2 cells - (reply: 1)
Floating cells in adherent VERO culture - contamination? (reply: 2)
RAW264.7 cells+nitric oxide determination - (reply: 13)
BL 21 cells - (reply: 4)
Mutated MDCK cells - protocol for mutating MDCK cells (reply: 1)
Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
Cell pellet pesuspension without Vortexing or pippeting - seeking advice - (reply: 2)
western blot problem: cell culture VS mouse tissue - (reply: 4)
How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 2)
Detachment of very adherent cells for flow cytometry - (reply: 7)
THP-1-Blue-CD14 cells - (reply: 4)
are cells prone to swelling in PBS after sorting - (reply: 3)
Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
suspension cell culture - optimum cell number in plates (reply: 2)
Whole tissue starting product - not cultured cells - (reply: 2)
DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
culturing COS-7 cells - (reply: 5)
Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
Loss of cell adherence after transfection - The first passage after selection process cells lost adherence (reply: 2)
how will i be able to culture tumor cells? - (reply: 3)
General cell lysis question - whole cell lysates need to be centrifuged? (reply: 2)
Rnase for cell cycle - (reply: 5)
Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
cell loss upon centrifugatino - (reply: 10)
cell lines vs tissue expression - (reply: 1)
Is molecular biology grade DMSO good enough for cell stock? - (reply: 2)
Clonogenic assay with agressive cell line - (reply: 4)
e coli cell lysis - heterologous gene expression in e coli (reply: 3)
Number of cells in 6 wells-plate well - (reply: 1)
protein extraction from primary cells - (reply: 1)
Extracting proteins from cells - (reply: 1)
Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 4)
cell floating in 96-well plate - (reply: 1)
Inducing Adherence in Suspension Cells - (reply: 1)
Isolated nuclei and trypan blue - Silly question .......... (reply: 2)
bacterial contamination in cell culture - (reply: 3)
THP-1 cell culture - (reply: 1)
H9c2 cell contamination? - (reply: 4)
Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
Inducible expression in human cell line - suggestions for a good system? (reply: 4)
What stains red in trypan blue - (reply: 2)
transfection in floating cells - (reply: 4)
immortal or stable cells - primary human cells to immortal cell line? (reply: 3)
SDS-PAGE Cell lysis problems - (reply: 3)
Storage of invitrogen top10 cells - (reply: 11)
oxidative stress - how to measure oxidative stress in cells (reply: 2)
Cells clots forming during preparation mouse splenocytes - (reply: 4)
Transwell Cell Migration/Invasion Assay - (reply: 4)
neuronal nitric oxide synthase/cell line - (reply: 3)
HL 60 cells passage - (reply: 1)
Establishing a new cell line - (reply: 1)
Isolating Natural Killer cells from whole blood - (reply: 2)
Centrifugation of epithelial cells - (reply: 2)
cell engineering exam - (reply: 5)
Cell detachment: alternative to trypsine/EDTA? - (reply: 3)
Cell supplier - (reply: 2)
Fc Blocking in cell cultures - (reply: 1)
Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
Mir34a positive control cells - (reply: 4)
Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
FACS positive cells in negative controls help - (reply: 1)
Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
How to separate fungus cells from human lung tissues - (reply: 7)
Growing of TOP10 chemically competent cells - (reply: 4)
Stable Cell Selection with Neomycin - (reply: 4)
endogenous miRNA of cos-7 cell - (reply: 2)
Qustion about MDA-MB-157 cells culture - (reply: 2)
Folic acid solution for cell culture - (reply: 2)
Mycoplasma contamination - (reply: 6)
Do RAW264.7 cells express both MHC II alleles? - (reply: 1)
Strange dots around the cell+Photo - (reply: 6)
FACS of GFP expressing cells from mice brains - (reply: 1)
cells can't attach in the flasks - (reply: 4)
Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
Cell fractionation and isolation of nuclei - (reply: 1)
Methanol toxicity in cell culture - (reply: 15)
Freezing cells after fix & perm and also a BrdU question - (reply: 4)
Freezing cells, quick question - (reply: 1)
Best place to buy cells - (reply: 2)
help on generation of stable cell lines with pBabe vector - pBabe-puro, phoenix Ampho cells (reply: 7)
DMSO usage in cell culture - (reply: 9)
stopping brief cell treatments - (reply: 2)
Phenol red in media - What metabolic products of cells are acidic? (reply: 2)
Cell culture container - (reply: 2)
cell lysis method - (reply: 1)
Dendritic cell immunology - activated naive cells? (reply: 9)
cell contamination???? - (reply: 1)
counting number of cells - (reply: 6)
Stacking of RAW 264.7 cells - (reply: 1)
retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
Lentiviral transduction of B cells - (reply: 7)
Lentivirus transduction of spleen cells - (reply: 2)
trouble with establishing a stable cell line - (reply: 3)
Cell culture conditions and antigen expression - (reply: 1)
Phoenix packaging cell and diphtheria toxin - (reply: 3)
Gene expression in cell line - (reply: 1)
isoflurane and FACS analysis of peripheral blood? - my cells look terrible everytime I use isoflurane to anaesthetize (reply: 5)
Growing Up Cells - (reply: 3)
protein extraction after cell lysing - (reply: 2)
cell line for neurodevelopment - (reply: 2)
Difficulty in doing hemocytometer cell count - (reply: 4)
No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
Method for lysing cells for fractionation - (reply: 4)
Dying Monocytes - Problem with cell culture of monocytes (reply: 1)
Neuronal cell culture - why use neonatal brains? - (reply: 1)
Detect gene in stable cell line, - (reply: 4)
staining cells - (reply: 3)
pIRESneo for mammalian cells transfection - (reply: 4)
Protocol for staining live cells - Staining live cells for Flow (reply: 2)
H9C2 - cell line - Need clarification (reply: 1)
Cell plating calculation !! - Help needed with Cell plating calculation !! (reply: 2)
Cell lines to study epigenetics - (reply: 2)
How to transfect two plasmids in a cell - (reply: 10)
Cells dying before i can get them to in vitro assay - (reply: 1)
Strange bands in western of RPE-1 cells - (reply: 1)
Semisolid Medium For HL 60 cells - (reply: 4)
Cell culture contamination (do not know what it was) - (reply: 1)
questions about cell lysis - (reply: 7)
Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
How to homogenize cell without disruption of nuclei? - (reply: 2)
Harvesting cells for flow cytometry - (reply: 10)
culture C6/36 insect cell lines - (reply: 1)
seeding cell densities - seeding cells at same density (reply: 1)
cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
Culturing QT6 cell line - Ideal culture conditions (reply: 2)
Calculating MOI for luciferase-transducted cells. - Would the sensitivity of a fluorescent microscope be enough to see the (reply: 3)
Picking epithelial, endothelial and neural cell lines - Please suggest best models (reply: 2)
How to fix suspencion cells for DAPI detecting under the microscope? - (reply: 6)
mycoplasma growth - (reply: 3)
primary lung epithelial cells - (reply: 1)
Generating double stable cell line - (reply: 3)
HACAT cells - very low cell viability after thawing cells (reply: 18)
cell line for luciferase assay - How to choose the best cell line? (reply: 2)
My cells suddenly die !!! - Please help me ! why is this ? (reply: 11)
database for mutations in cancer cell lines? - (reply: 2)
Sonication Problems, Branson 450, LNCaP cells - (reply: 4)
? Definition of Passage for suspension cells - (reply: 1)
Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
Chip problem: antibody or cell number?? - (reply: 6)
stable cell line - (reply: 1)
microbial cell viability - any recommendations - assays (reply: 1)
SF9 cells not adhering - (reply: 10)
HeLa cells - Growth of thawed HeLa cells (reply: 7)
Dendritic Cells and Flow Cytometry - (reply: 11)
direct cell lysate - (reply: 8)
How long can I wait after seeing HeLa cells till transfection - will it affect my Efficiency (reply: 6)
Culturing/Mounting of polarised cells for confocal microscopy - (reply: 1)
Intestinal cell culture: Protocol required - (reply: 1)
Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
Carbon di oxide free cell culture - (reply: 1)
surface staining of THP-1 cell - (reply: 2)
How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
Cell Wall Lysis For Dummies - (reply: 4)
Tissue & Cell Fixation solutions - (reply: 3)
MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
Treating cells with chemicals and how to dilute into medium - (reply: 5)
T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (reply: 6)
Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
Has anyone used COS-7 cells for retina? - (reply: 1)
How to perform a growth curve? - i need to compare 3 cell lines for growth (reply: 2)
siRNA concentration in divinding-growing cells - (reply: 2)
siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
cell culture guideline from abcam - (reply: 5)
Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
problems with detecting protein expression - protein expression in cell (reply: 4)
freeze PBMC and cell sorting? - (reply: 1)
Control vehicle treatment in cells - (reply: 2)
Cancer cell culture exploded onto my face - (reply: 8)
FACS analysis of GFP expressing cells - Fix or not - (reply: 10)
HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
Heterogeneous methylation in a same cell line - (reply: 1)
siRNA cell function examples - (reply: 1)
Good comprehensive resource for maintaining immortal cell lines? - (reply: 1)
DH5a comp cells - (reply: 5)
Trypsinized cells after fixation - (reply: 3)
Detaching Hela cells - (reply: 3)
help! Truncated protein cannot expressed in mammalian cells? - (reply: 5)
cell cycle softawe - (reply: 3)
How to maintain cells at a specific apoptosis stage - (reply: 12)
Which cell line produce cytokine? - (reply: 13)
Cell Viability - (reply: 1)
Shipping of cryopreseved cell - (reply: 15)
To what extent competent cells be thawed before transformation? - (reply: 4)
Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
Storing HEK293 cells at -80C - (reply: 5)
How to control cloned cDNA expression level in cells? - (reply: 8)
Asses mycoplasma contamination - (reply: 6)
RNA isolation following apoptotic cell labeling - (reply: 3)
Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
Is there an optimal incubation time for cell lysis? - (reply: 7)
The way to count adherent cells in 96 well - (reply: 3)
lysing cells in LSB - (reply: 2)
Using NIH3T3 cells of look at MAPK activation by Ras - (reply: 1)
Staining for neural crest derived cells?? - (reply: 2)
Dendritic_ cells - (reply: 3)
problem to stain just one cell line in coculture - (reply: 4)
Inactivation of trypsin - (reply: 4)
Culturing stromal cells - (reply: 2)
A549 cells - (reply: 4)
Cunterstaining Trypan blue - (reply: 6)
Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
Protein extraction from cell culture media - (reply: 9)
Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
can we prepare Copmetent cell by calicum cloride with 0.09 OD - Cp cells with 0.09 OD (reply: 3)
electroporation of HL60 cells - (reply: 1)
Intestinal epithelial cell culture - (reply: 4)
SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
Easiest cell lysis protocol - no interest in preserving proteines (reply: 4)
passage number for cell line - (reply: 7)
MOCK INFECTED CELLS - (reply: 4)
cuboidal cells - (reply: 1)
Cell count using a hemocytometer - (reply: 4)
Hepatocyte culture - (reply: 4)
Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
what is mock cell line? - (reply: 3)
293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
How to seed the cells evenly in 6 wells plate - (reply: 13)
knockout mouse with increase cell size - (reply: 3)
Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
Paper on energy requirement of cells - (reply: 2)
galactose induction of yeast cells - (reply: 2)
making competent E coli DH5a cells - (reply: 6)
How could you explain these results? - cancer cells are weird (reply: 1)
strange ES cells contamination - please help!!! (reply: 1)
Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)
How to culture PC3 cell line - (reply: 3)