Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Cell culture Related Discussions
  1. ¿must treatment be aplied 24h post seed the cells? - (reply: 1)
  2. Question about transfecting siRNA into cells - (reply: 1)
  3. Mammalian Cell Lysis - (reply: 1)
  4. 293T cells for lentivirus production - (reply: 1)
  5. Counting and seeding cells?]. - (reply: 1)
  6. propagating commercial chemically competent cells - (reply: 2)
  7. CRISPR-Cas9 / insert a gene in cell line genome - (reply: 1)
  8. Beginner Cell and Tissue, and Molecular Biology methods reading list - (reply: 1)
  9. how to present Michaelis-Menten equation in cell designer - (reply: 1)
  10. TCDD (2,3,7,8-​Tetrachlorodibenzo-p-dioxin solution) cell line treatment - (reply: 1)
  11. cell invasion assay - (reply: 1)
  12. Help with the Red Blood Cell Lysis - (reply: 1)
  13. gene expression in cultured cell lines - (reply: 1)
  14. Splitting cells without centrifugation - (reply: 4)
  15. Are HEK293 cells a positive control for wild type p53 expression? - (reply: 1)
  16. Culturing LoVo cells - (reply: 1)
  17. Black dots contaminant in stable cell lines - antibiotics - (reply: 1)
  18. cell viability assay for caco-2 and pbmc - (reply: 1)
  19. Subculturing colonies to agar - (reply: 2)
  20. 3T3 L1 cell culture - (reply: 1)
  21. Why my bacteria cells look weird in DAPI fluorescence? - (reply: 2)
  22. B & T cell activation in autoimmune disorders - (reply: 1)
  23. lentiviral transduction in BLCL cells - (reply: 3)
  24. Synchronization of HEK293T cells for IP - (reply: 2)
  25. Alternate cell line not giving same results as first - (reply: 1)
  26. Cell volume and reciprocal cell volume? Whaaat? - (reply: 1)
  27. How to detect specific-protein producing cells? - (reply: 8)
  28. Who came up with 50-100 IU/mL Antibiotic working concentration in cell culture? - (reply: 2)
  29. Help! Antibiotic and Serum Concentration in Cell Culture Media - (reply: 1)
  30. Large-scale cell growth and protein expression - (reply: 1)
  31. is cell signaling difficult? - (reply: 3)
  32. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
  33. The adipose cell culturing - (reply: 2)
  34. Recommendation of cell signaling inhibitors - (reply: 2)
  35. Recovering cells from ancient sample - (reply: 3)
  36. Microinjection of cells into the lumen of spheroids ? - (reply: 1)
  37. Human cell membrane protein Western blot! - (reply: 2)
  38. Cell Line confluence level - (reply: 1)
  39. too much cell density can influence adhesion to culture plate - (reply: 1)
  40. Shipment conditions for cultured cells - (reply: 3)
  41. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
  42. E-Cadherin Expression in OAW-42 Cells - (reply: 1)
  43. Why HepG2 cells looked like that? - (reply: 2)
  44. Trouble transfecting HEK 293 T and HUH 7 cells - (reply: 1)
  45. 3T3-L1 cell contamination - (reply: 3)
  46. Will lipofection of DNA work in post mitotic cells - (reply: 1)
  47. Cell death determination - (reply: 2)
  48. Culture Tubes for CHO cells - (reply: 1)
  49. SHSH5Y cells - (reply: 1)
  50. Is the mycoplasma that infects cell culture dangerous to humans? - (reply: 1)
  51. Distinguishing two populations of cells by TEM - (reply: 4)
  52. transfection of suspended CHO cells - (reply: 2)
  53. using IC50 to determine dosing of inhibitor in cell culture - (reply: 2)
  54. Software for automated cell counting? - (reply: 2)
  55. why is my cells dying in the centre of the wells after transfections - (reply: 1)
  56. How to establish drug-resistant cell lines? - (reply: 1)
  57. Are normal or immortalized human epithelial cells clonogenic? - (reply: 1)
  58. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
  59. Fibroblast Cell Culture Issue - Cells grouping together and cell clusters observ - (reply: 3)
  60. pUC and pSC101 vectors in the same cell - (reply: 3)
  61. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
  62. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
  63. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
  64. Is cultured cell reuse possible? - (reply: 1)
  65. Count cell bactria using haemacytometer - (reply: 1)
  66. Help with HUVEC cell transfection - (reply: 3)
  67. Cell clumps or contamination? Please help! - (reply: 1)
  68. How to clean up cell fragments before cell sorting - (reply: 1)
  69. Is any other method to maintain without changing media, or store cell culture fo - (reply: 2)
  70. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  71. Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
  72. Abnormality in PMA treated THP1 cells - (reply: 1)
  73. Preferred assay for measuring cytotoxicity or cell proliferation - (reply: 2)
  74. Cell lysis - (reply: 1)
  75. Can foam created when resuspending a cell pellet cause cell demage - (reply: 1)
  76. Distribution of cell receptors on the cell surface - (reply: 3)
  77. MTT Cell Treatment - (reply: 1)
  78. cells behaving differently - (reply: 4)
  79. Cell lines behaving differently to drugs - (reply: 2)
  80. How to achieve a uniform monolayer with HepG2 cells, seeded in 24 welll plate fo - (reply: 1)
  81. Efficient lysis of E.coli cells - (reply: 4)
  82. Alternative competent cells for QuikChange kit - (reply: 2)
  83. unknown bodies in cell culture - (reply: 1)
  84. Unbroken cell - (reply: 1)
  85. how to confirm lentiviral integration in a target cell line - (reply: 1)
  86. Invasion assay: analysis question: round cells - (reply: 2)
  87. Measuring ammonia in cell lysates - (reply: 1)
  88. Methanol oor PFA to fix cells for nuclear protien analysis by immunofluorescence - (reply: 4)
  89. What happens when trypsin is added to cells? - (reply: 1)
  90. Selective evolution of cells for cancer - (reply: 4)
  91. cell line - (reply: 3)
  92. I want know what companies are producing Biosimilar cell line - (reply: 1)
  93. contamination in HepG2 cell culture - (reply: 1)
  94. Which neuroblastoma cell lines grow the longest axons - (reply: 1)
  95. Help with Question for Cell Bio Take Home Exam - (reply: 2)
  96. leukemic cell lines 697 - (reply: 1)
  97. where do you get cells needed for your research - (reply: 1)
  98. PCR with mammalian cells as template - (reply: 1)
  99. Dislodge cell in 96well plate - (reply: 1)
  100. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  101. cell counting - (reply: 2)
  102. What do the glioma cell line prefixes LN and U stand for ? - (reply: 1)
  103. Trypan Blue Staining - (reply: 1)
  104. colorectal cell culture - (reply: 2)
  105. Easy to transfect primary cell line? - (reply: 1)
  106. Sample prep of cells for FACS analysis and running WB - (reply: 4)
  107. Functional states of the same cell versus Different cell types - (reply: 2)
  108. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
  109. transfection of cells with two fluorescent reporters - (reply: 1)
  110. Stably transfected cells start dying - (reply: 6)
  111. U251 and U87 cells - (reply: 1)
  112. is -40 freezer enough for cell freezing? - (reply: 1)
  113. Experiment on nutrient surplus on Jurkat cell culture - (reply: 2)
  114. Help with HeLa cell cultivation - (reply: 5)
  115. skin lesion and low donor cell frequency in bone marrow transfer - (reply: 7)
  116. cell division rates - (reply: 6)
  117. Black dots-ghost cells or contamination? Please help - (reply: 6)
  118. Where do you purchase T cell hybridomas - (reply: 5)
  119. NK Cell Culture Selection and Expansion from PBMC - (reply: 2)
  120. 3D Cell Culture Matrix made of jelly / gelatin - (reply: 2)
  121. Cloning purified PCR product into cells - (reply: 5)
  122. Help ID fluoescently-labelled cells in the gut very much appreciated! - (reply: 2)
  123. Mention of Transgene Insertion into Cell Lines By Transfecting with cDNA - (reply: 2)
  124. Refreeze thawed cells? - (reply: 3)
  125. How can an animal cell obtain needed glucose when glucose is lacking in the diet - (reply: 2)
  126. Suppose a microorganism invades a cell, causing damage to ATP synthase. How woul - (reply: 3)
  127. How to capture live cells under microscope - (reply: 3)
  128. Identification of cells and tissue - (reply: 2)
  129. Research topic related to jurkat cell line - (reply: 3)
  130. What DCH5a cells are? / pCAMBIA1301 isolation from DHC5a cells - (reply: 3)
  131. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
  132. Assays from very few number of cells - (reply: 1)
  133. What I have to do If my active compound showing toxicity in normal cells? - (reply: 1)
  134. Passaging cells - (reply: 1)
  135. Problem in mouse embryonic stem cell culture - (reply: 4)
  136. Is there always a presence of activated T cells in a healthy host? - (reply: 1)
  137. Bacterial contamination in cell culture - (reply: 3)
  138. Cell culture contamination - (reply: 1)
  139. Contamination or dead cells? - (reply: 1)
  140. How to choose cell line - (reply: 4)
  141. Urea effect on mice spleen cells - (reply: 3)
  142. Urea effect on mice spleen cells - (reply: 1)
  143. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
  144. Best cryovials for liquid nitrogen storage of cells? - (reply: 2)
  145. Review articles on all types of mammalian cell culture - Any suggestion? - (reply: 1)
  146. 2 types of cell lines for RNA extraction - (reply: 1)
  147. live cell culture transport in 6 well plate - (reply: 6)
  148. Stem Cell Culture Theory - (reply: 4)
  149. Selectively arresting a subpopulation of biotinylated yeast cells. - (reply: 4)
  150. cell viability with PI staining - (reply: 2)
  151. trypsin digestion of 2D-PAGE protein spot - (reply: 2)
  152. Sonication of cells in ultrasound bath, without probe - (reply: 3)
  153. cell fractionation and microscopy - (reply: 3)
  154. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
  155. cell lysis problem - (reply: 2)
  156. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
  157. Thawing cells - (reply: 2)
  158. the same construct, two stable cell clones with different protein size - (reply: 2)
  159. Determine DNA sequence expression in cell line - (reply: 1)
  160. Harvesting cells - (reply: 3)
  161. Cell counters - Beckman Coulter Z1 counter - (reply: 1)
  162. Competent cells with Hanahan's method - (reply: 2)
  163. problem with cell synchronization - (reply: 4)
  164. can anyone tell me what kind of cell this is? - (reply: 3)
  165. Transfection of SH-SY5Y cells - (reply: 4)
  166. Protocol for isolating cells from Subcutaneous Tumors ? - (reply: 1)
  167. Hybridoma cells contaminated with large round cells! - (reply: 1)
  168. protocol for cell syncronization using colchicine - (reply: 2)
  169. PBMC cell stimulation? - (reply: 1)
  170. Caco-2 cells with unidentified contaminant - (reply: 2)
  171. MIN6 Cell Culture Contamination Risk - (reply: 1)
  172. Concentrating bacterial cells - (reply: 2)
  173. Would preserved cells for flow cytometry make your life easier? - (reply: 1)
  174. measure of cell size - (reply: 1)
  175. Transfecting cells for cell cycle analysis, how to deal with cell density? - (reply: 1)
  176. Splitting Cells that are shaped as small spheres - (reply: 2)
  177. Cells counting problem. - (reply: 2)
  178. Cell counts for Clonogenic and MTT assay - (reply: 3)
  179. Do I need to use untreated TC flask for suspension cell culture - (reply: 5)
  180. Cell cycle determination - (reply: 1)
  181. After centrifuge in serum-free media, forming cell sheet, instead of pellet in t - (reply: 2)
  182. Can cells be exposed to nanoparticles with ethanol as solvent??? - (reply: 2)
  183. Question about growth media for A20 Cell Line - (reply: 1)
  184. NSO cell culture - (reply: 1)
  185. Some queries regarding A549 cell culture - (reply: 1)
  186. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
  187. how to transport stem cells in suspension? - (reply: 2)
  188. animal cell culture - (reply: 1)
  189. help with contamination of cell culture - (reply: 2)
  190. Methanol concentration for cells? - (reply: 4)
  191. What happened to my cell? (Colony formation) - (reply: 7)
  192. problem of cell lysate - (reply: 3)
  193. Cell lysis and DNA quantification - (reply: 1)
  194. Best western loading control for comparing different cell lines - (reply: 2)
  195. B cell line - cell colour change and visible cell death - (reply: 1)
  196. Keeping apoptotic/detached cells for WB? - (reply: 3)
  197. Effective inhibitor of cell proliferation - (reply: 4)
  198. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
  199. Neural Stem Cells; does it mean adult or embryonic? - (reply: 1)
  200. Cell culture contamination or not? - (reply: 1)
  201. orthotopic mouse model of U251 cell line - (reply: 4)
  202. How to release the bacterial cells from filter paper? - (reply: 4)
  203. Cell adhesion/detachment - (reply: 1)
  204. methylation observation in normal cell line - (reply: 1)
  205. Stem cells created by dipping researchers into coffee - (reply: 2)
  206. Freezing yeast cells - (reply: 4)
  207. codon optimization for CHO cells - (reply: 2)
  208. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  209. chk effect of cell line genes on normal dna - (reply: 1)
  210. Would someone help me discuss what work can I do with laryngeal cancer cell hep2 - (reply: 12)
  211. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
  212. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
  213. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
  214. CHIP on frozen cell pellet - (reply: 1)
  215. UV Light benefits and costs on Cells - (reply: 2)
  216. Adapting a Drosophila cell culture to Zebrafish for subsequent MACS-sorting - (reply: 3)
  217. mouse embryonic stem cell culture on gelatine coated plates - (reply: 1)
  218. easiest cell lines to use - (reply: 4)
  219. Jurkat cell - (reply: 4)
  220. Mesenchymal stem cells Expressing BDNF - (reply: 5)
  221. PI staining cell cycle analysis - (reply: 1)
  222. Protein Purification from mammalian cells - (reply: 1)
  223. How to identify CD8 T cell receptor for a particular antigen - (reply: 2)
  224. How to inactivate H1N1 virus in Cell culture supernatant ? - (reply: 3)
  225. HaCaT cell line - not responding to inflammatory stimuli such as LPS/TNF-alpha - (reply: 4)
  226. Homogenous Cell Lysates - (reply: 1)
  227. Does anyone irradiate their cells? - (reply: 3)
  228. need help for transfection to synchronized cells - (reply: 4)
  229. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
  230. What should I suspend my cells in prior to cytometry? - (reply: 2)
  231. Checking for efficieny competent cells - (reply: 1)
  232. Video on cell culture technique (broad brush) - (reply: 2)
  233. Setting up two drug kill curve for selection of stable cells - (reply: 1)
  234. No expression in stable transfected cell line - (reply: 2)
  235. Protocol for flow cytometry on mononuclear cells from mouse blood - (reply: 1)
  236. Cell line contamination? - (reply: 5)
  237. Inducing apoptosis by puromycin selection: toxic to the selected cells? - (reply: 1)
  238. Measuring cell proliferation from daily aliquots - (reply: 1)
  239. Cell culture of MYC immortalised HDFa - need advice - (reply: 1)
  240. Phoenix Amphoteric cell line to package lentivirus? - (reply: 6)
  241. miR30 based shRNA in mouse cells - (reply: 3)
  242. Cells are unhealthy - (reply: 2)
  243. Harvesting cells - (reply: 12)
  244. Dead cells after transfection - (reply: 9)
  245. preparation of cells for FACS analysis after transfection - (reply: 4)
  246. simultaneous knock down of two genes in a single cell line - (reply: 1)
  247. CoIP from cell lysate mix - (reply: 5)
  248. selective pressure on cells - (reply: 2)
  249. Conjugation of Ab to magnetic beads to isolate T cells from Spleen - (reply: 2)
  250. cell culture and biopsy - (reply: 1)
  251. Tiny black dots inside cell vacuoles - (reply: 1)
  252. I always have a cell depris floating in the supernatent of MDCK cells - (reply: 3)
  253. Is Trifluoroacetic acid toxic for cells? - (reply: 5)
  254. mycoplasma testing - (reply: 1)
  255. Treating suspension cells - (reply: 2)
  256. H9c2 cells..please help - (reply: 2)
  257. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
  258. How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (reply: 1)
  259. Establishing human neural stem cells - (reply: 3)
  260. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  261. Cell Adhesion and Motility on Fibronectin vs Collagen - (reply: 1)
  262. FCS in Serum free medium for better Cell Adhesion? - (reply: 1)
  263. Possible to sort cells from formalin? - (reply: 1)
  264. 293s vs HEK/293 cells - (reply: 2)
  265. Cell culture medium contamination? - (reply: 1)
  266. What do sick 293t cells look like? - (reply: 3)
  267. H2DCFDA and fixation of cells before flow cytometry analysis - (reply: 4)
  268. Decreasing cell division - (reply: 1)
  269. Can I use methanol fixation to fix cells before proteomic analysis? - (reply: 1)
  270. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  271. Do S2 cells normally have small 1-2um objects floating in culture? - (reply: 1)
  272. Problem in Cell Viewing - (reply: 3)
  273. How to view the encapsulated cells under microscope? - (reply: 5)
  274. Poor data quality using cultured cell in aCGH - (reply: 10)
  275. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  276. Totipotent Stem Cell From Molecular Components - (reply: 5)
  277. How to count clumpy epithelial cells? - (reply: 5)
  278. Why cells are floating after incubation in EBSS?? - (reply: 1)
  279. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  280. Live cell sort DNA stain permeabilization - (reply: 1)
  281. Problem in bacterial cell culture - (reply: 12)
  282. Persistent contamination in cell culture - (reply: 9)
  283. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  284. Adult stem cells - (reply: 2)
  285. Centrifuge stopped working while my blood samples were incubated in Cell lysis s - (reply: 1)
  286. Ways to Quickly Identify Antibiotic Resistance in Bacterial Cells (Non-tradition - (reply: 12)
  287. freezing competent cells without dry ice or liquid N - (reply: 3)
  288. Suspension cell line (RPMI) adhere to flask-WHY? - (reply: 1)
  289. Rapamycin Concentration for Cell Culture - (reply: 1)
  290. how to calculate cells and plate - (reply: 2)
  291. yeast conatamination in primary cells - (reply: 4)
  292. Maltodextrin will kill all the bacterial cells? - (reply: 5)
  293. Keratinocyte Culture: Cells detach - (reply: 7)
  294. Measure growth of plant cell culture - (reply: 1)
  295. Cell Lysate Western Blot Little or No Signal - (reply: 7)
  296. Cell line identity (HEK293-E) - (reply: 1)
  297. How do I calculate the enery absorved by cells form a UV light - (reply: 3)
  298. Immunocytochemistry - Washing away PC12 cells - (reply: 2)
  299. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
  300. Total Protein in COS-7 Cells - (reply: 1)
  301. protein extraction from cell nucleus - (reply: 2)
  302. Which gauge for proper shear force on certain cell dimension - (reply: 1)
  303. Mammalian Cell Lysis Buffer Triton-X100 Content - (reply: 6)
  304. DNA from frozed cells??? - (reply: 2)
  305. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
  306. Tumor cell analysis - (reply: 2)
  307. What can I do with my MCF7 cells? - (reply: 1)
  308. Evolution of Living Cells from Non-living Organic Matter - (reply: 3)
  309. cell lines - (reply: 5)
  310. M2 antibody background in Xenopus cell extracts - (reply: 6)
  311. Asking for guidelines to start L6 cell line culturing - (reply: 1)
  312. how to separate organelles from mammalian cells - (reply: 1)
  313. Cell culture contamination ? - (reply: 5)
  314. How to coat 96 well plate with poly-L-Lysine for cell culture use? - (reply: 2)
  315. Can serum free medium inactivate trypsin? - (reply: 1)
  316. Is Protein A present on the Staphylococcus saprophyticus cell wall too? - (reply: 2)
  317. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
  318. red dots in cell culture line? - (reply: 3)
  319. Integrity of cell monolayer - (reply: 1)
  320. problem with COV318 ovarian cancer cell line - (reply: 4)
  321. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  322. atom replacement in human cells - (reply: 2)
  323. What stage of growth are the cells I counted? - (reply: 4)
  324. HeLa cell culture - (reply: 6)
  325. Lambda Phosphatase Treatment of Cell Lysate - (reply: 1)
  326. Problem in Determination of Total Bacterial Cells - (reply: 13)
  327. Preparing a cell culture stock to plate out at 7x10^5 per well? - (reply: 1)
  328. Cell culturing tips - (reply: 2)
  329. SAOS.2 Cell detachment without using Trypsin. - (reply: 3)
  330. Normal cells or contamination?! - (reply: 4)
  331. H2DCFDA for MDCK cells - (reply: 2)
  332. immortalized human neuronal cells - (reply: 4)
  333. LNCaP Cell Clusters - (reply: 2)
  334. Freezing viable cells in 96-well format? - (reply: 3)
  335. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  336. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  337. Does fixation and permeabilization of cells affect their oxidative stress levels - (reply: 1)
  338. HBE cell culture CRL-2079 MEDIA question - (reply: 1)
  339. Cells dying in culture after 24 hours - (reply: 6)
  340. SH-SY5Y cells: basics advice - (reply: 1)
  341. Cell clumping: dead cells? - (reply: 6)
  342. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  343. growing cometent cells - (reply: 1)
  344. Where can I purchase HepG2.2.15 cell line - (reply: 1)
  345. Mycoplasma detection with ATCC kit - (reply: 1)
  346. RAW 264.7 cell line - (reply: 1)
  347. Mycoplasma infected cell line clean-up - (reply: 1)
  348. Rheumatoid Factor (RF) producing cell line? - (reply: 1)
  349. Weighing harvested cells - (reply: 1)
  350. Temperature Variation on Cell Growth Experiment - (reply: 1)
  351. Morphology of cultured cells - (reply: 5)
  352. Growing GH teratocarcinoma cell line- help needed - (reply: 2)
  353. Cells Detach After Transfection - (reply: 1)
  354. How to determine the location of integrated DNA in stable cell lines - (reply: 2)
  355. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  356. When cell media becomes "used up", it acidifies - (reply: 3)
  357. Cell cycle analysis for SubG1 fraction - (reply: 3)
  358. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  359. Cytokines feeding cell culture - (reply: 1)
  360. Experience with U-937 cell line - (reply: 3)
  361. Cell culture contamination issue - unable to identify the contamination - (reply: 1)
  362. cell confluency in IF - (reply: 2)
  363. Protein purification for adherent mammalian cells - (reply: 2)
  364. Antibody production in Expi293 cells - (reply: 2)
  365. death of transfected cells after first passage in G418 medium - (reply: 1)
  366. clonogenic assay for shRNA transfected cells - (reply: 3)
  367. Cell lysis in chromosome conformation capture - (reply: 2)
  368. Relationship between cell lines and cancer research (IHC) - (reply: 4)
  369. Serum starvation - something similar for serum-free kept cells ? - (reply: 2)
  370. Sodium bicarbonate addition to GMEM for BSR T7/5 cells - (reply: 2)
  371. No proteins after HeLa cell lysis - (reply: 3)
  372. How can i fix cultured cells on glass slides for insitu hybridization - (reply: 1)
  373. Blocking a protein in cell culture through antibody - (reply: 10)
  374. How to propagate Electro-competent DB3.1 cells - (reply: 6)
  375. Number of cell - (reply: 5)
  376. Difference between PFA, acetone and methanol in cell fixation - (reply: 1)
  377. cell proliferation statistics methods - (reply: 4)
  378. Losing cells during fixation - (reply: 2)
  379. ovarian cancer cell line: phenotype disappears - (reply: 3)
  380. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  381. HeLa Cells on Chin/in Nose? - (reply: 7)
  382. Can centrioles be seen when the cell is not undergoing cell division? - (reply: 3)
  383. How much ethanol (in micro g) has toxic effects on cells? - (reply: 1)
  384. penicillin/streptomycin in cell culture - (reply: 3)
  385. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
  386. Cell lysis for unknown reasons - (reply: 1)
  387. Preparing cells for plating - (reply: 3)
  388. ChIP with low cell number - (reply: 2)
  389. Mammalian cell culture and G418 selection - (reply: 1)
  390. Losing cells during intracellular staining method! - (reply: 1)
  391. G418 mammalian cell selection question - (reply: 3)
  392. DIFFERENCES BETWEEN GLIAL AND GLIOMA CELL? - (reply: 4)
  393. Bought HEK293T cells from ATCC -- passage number question - (reply: 1)
  394. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
  395. cell pellet question - (reply: 2)
  396. B cells cultures. Immunoglobuline production - (reply: 1)
  397. ChIP problem - using cells as a negative control - (reply: 7)
  398. Blood cell migration from mother to fetus - (reply: 1)
  399. Qiagen mini prep cell fragment pellet - (reply: 2)
  400. Cell death detection using adenosin kinase - (reply: 5)
  401. THP-1 differentiation using PMA yielding black dots within cell structure - affe - (reply: 4)
  402. Transfecting three plasmids into hES cells - (reply: 2)
  403. Agglomeration of suspension cells (T-cells) - (reply: 1)
  404. Selection of E.coli competent cells - (reply: 2)
  405. Chelating Aluminum from cell culture - (reply: 5)
  406. chemotherapeutic-resistant cell lines - (reply: 1)
  407. infection assay-buffer that lyses mammalian cells but not bacteria - (reply: 1)
  408. HEK293 vs. SH-SY5Y Cell lines? Which best for in vitro Alzheimer's Model? - (reply: 2)
  409. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
  410. Electroporate lentiviral vector for stable cell line - (reply: 2)
  411. Place to buy (soluble) ligands for cell culture. - (reply: 3)
  412. NIH 3T3 cells cross contamination - (reply: 1)
  413. NIH 3T3 cells - (reply: 3)
  414. Cells adhering to walls of conical tube? - (reply: 7)
  415. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  416. choosing the correct subculturing ratio - ovarian cancer cell lines - (reply: 1)
  417. RAW 264.7 cell line - (reply: 4)
  418. washing of attached bacterial cells - (reply: 3)
  419. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
  420. Cell Debris Isolation From Blood - (reply: 3)
  421. How to calculate drug amount to use in cell lines - (reply: 3)
  422. Growing certain number of bacterial cells in LB - (reply: 1)
  423. T cells or Lymphocytes? - (reply: 2)
  424. Western Blot with fixed cells - (reply: 5)
  425. Less than 10 GFP positive cells - (reply: 2)
  426. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
  427. How to culture HK-2 cells - (reply: 6)
  428. competent cells - (reply: 1)
  429. Debris in cell culture - (reply: 2)
  430. What housekeeper to use with HEK cells? - (reply: 3)
  431. Housekeeper for HEK cells? - (reply: 1)
  432. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
  433. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
  434. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
  435. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
  436. Gene expression profiling with small cell number - (reply: 1)
  437. Gene expression assay with small cell number - (reply: 3)
  438. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
  439. Stem Cell Therapy comes of age? Discussion Topic - (reply: 1)
  440. cell free DNA and maintaining stability - (reply: 3)
  441. Freezing Cells For Use In Assays - (reply: 2)
  442. INS-1E cells migration assay - (reply: 1)
  443. Short-term storage of HEK293 cells before FACS - (reply: 3)
  444. Sample storage for ELISA (cell culture supernatant) - (reply: 2)
  445. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
  446. Primary cell line - (reply: 5)
  447. 2D Image Primary Cell line - (reply: 13)
  448. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  449. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
  450. To small amount of cells used for shRNA transfection - (reply: 1)
  451. using cell line to generate standard curve - (reply: 6)
  452. Autofluorescence in thick structure cluster of cells - (reply: 4)
  453. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
  454. Serial dilution of cells - (reply: 3)
  455. Cloning repetitive sequences in mammalian cells - (reply: 3)
  456. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
  457. Plant Cells - what are they? - (reply: 1)
  458. HS-5 and HS27A cells - (reply: 1)
  459. MC-3T3 cells are not mineralizing... - (reply: 3)
  460. HS-5 cell culture "question" - (reply: 1)
  461. Problem with Kasumi-6 cell line - (reply: 1)
  462. G418(Geneticin) is not killing my cells - (reply: 2)
  463. Community feedback needed to clarify terms for a Cell Culture Ontology - (reply: 1)
  464. Quantify total DNA in small cell lysate...? - (reply: 1)
  465. Trouble validating in multiple cell types - (reply: 1)
  466. Gating of dendritic cells - (reply: 7)
  467. Please help me take a look at my cells to see whether is it contaminated - (reply: 3)
  468. cell binding assay with nanoparticles? - (reply: 2)
  469. Cell lines for clonal selection - (reply: 3)
  470. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
  471. SH-SY5Y Immortalized cell lines; researching neurotoxicity? - (reply: 3)
  472. Pelleting bacterial cells - (reply: 1)
  473. How viable is cell culture for a model? - (reply: 2)
  474. Help with adherent cell culture - (reply: 7)
  475. study of receptors in cell lines - (reply: 1)
  476. Cell line nomenclature - suffixes ? - (reply: 3)
  477. Maintainence of transformed cells - (reply: 5)
  478. improving viability of pelleted cells - (reply: 2)
  479. Buying Ins-1e cells - (reply: 1)
  480. How do/should cell biologists calculate power of analysis? - (reply: 1)
  481. Mycoplasma on normal hands? - (reply: 1)
  482. comparing ADCC effect in 3 cell lines. - (reply: 1)
  483. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
  484. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
  485. Trypan blue - (reply: 6)
  486. virus and cell surface receptor interaction - (reply: 4)
  487. Music Video on Cell Division/Mitosis - (reply: 3)
  488. How to select competent E. coli cells? - (reply: 7)
  489. Host cell protein sequence search - (reply: 1)
  490. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  491. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  492. Preparing supernatant from cell culture for western blot - (reply: 4)
  493. Fast/easy way to measure cell size - (reply: 4)
  494. Which is the best way to generate growth curves for cancer cell lines - (reply: 7)
  495. Cell number issue - Nile Red staining - (reply: 3)
  496. Music Video on Cell Division/Mitosis - (reply: 1)
  497. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
  498. Integrin B1 Staining in HT1080 cells - (reply: 1)
  499. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
  500. My cells won't pellet - any ideas? - (reply: 6)
  501. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
  502. EMSA: cell extract vs purified protein - (reply: 2)
  503. Insect cells transfection reagent - (reply: 1)
  504. Low Protein Content In Cell Lysate - (reply: 1)
  505. Can you see GFP after lysis mammalian cells? - (reply: 5)
  506. Centrifugation speeds for cells. - (reply: 8)
  507. Cell recovery from ethanol fixation - (reply: 4)
  508. Cryopreservation of MSC at lower cell density - (reply: 2)
  509. C-terminal GST fusion problem in mammalian cell - (reply: 6)
  510. Identify cell culture contamination - (reply: 2)
  511. Adipose stem cell culture - (reply: 5)
  512. Determine cell proliferation by viability assay ? - (reply: 6)
  513. Apoptosis via cell to cell contact - (reply: 1)
  514. Cells stop to grow - (reply: 6)
  515. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
  516. Long Treatment, Rapidly Dividing Cells - (reply: 7)
  517. Need help calculating cell number to plate - (reply: 1)
  518. Injection of human cell culture conditioned media into nude mice - (reply: 1)
  519. How long can I store cell lysis buffer at 4C for? - (reply: 1)
  520. Heeeeeelp..cell cycle sample..is it possible to store it? - (reply: 2)
  521. Subculture issue of primary cell - (reply: 6)
  522. Differentiating mouse from human cells with PCR - (reply: 5)
  523. primary valve interstitial cells transfection - (reply: 1)
  524. What to do about mycoplasma? - (reply: 9)
  525. Stem Cell Donation - (reply: 7)
  526. isolating DNA from cells in suspension - (reply: 5)
  527. Software for designing cell pathways - (reply: 1)
  528. Tamoxifen citrate solubility for cell work?? - (reply: 1)
  529. Centrifuged cells stick to wall of reaction tube - (reply: 12)
  530. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  531. Mycoplasma Test Kit - (reply: 1)
  532. Changing Genomic Regions in Host Cell - (reply: 1)
  533. TRYPSIN - (reply: 4)
  534. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
  535. my competent cell grow on any agar plate - (reply: 7)
  536. Bacterial Contamination in HuEmbryonic cell lines seen in SFM4 but not in DMEM + - (reply: 3)
  537. Cell attachement occuring after 48 hours, not 24 hours. - (reply: 3)
  538. Transfection of a non human gene into human cell line - (reply: 1)
  539. Procedures to replace vial of cells and passage number. - (reply: 4)
  540. Immunofluorescence on cells - can I keep the coverslips unstained in PFA? - (reply: 4)
  541. p14ARF and MDM2 in transformed cells - (reply: 1)
  542. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  543. contamination of human cell lines with yeast - (reply: 3)
  544. Generation of single cell suspensions from neural tissue - (reply: 2)
  545. Is this contamination or cell death? - (reply: 7)
  546. BaF3 cell culture in 96 well plates - (reply: 2)
  547. THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS) - (reply: 2)
  548. unwanted cell lysis during LB+Amp incubation - (reply: 5)
  549. Cells spontaneously detaching? - (reply: 1)
  550. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
  551. different type of media for cell culture - (reply: 1)
  552. Influenza: cell and mechanism information? - (reply: 1)
  553. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
  554. How to treat cells with TNFa - (reply: 2)
  555. CLOUDINESS of RIN5F cell culture - (reply: 7)
  556. U87MG cells not growing well - (reply: 3)
  557. Concentrating low volume of cells - (reply: 8)
  558. Preparing a conditioned medium from cancer cells - (reply: 3)
  559. Presence of melanin in melanin containing cell line - (reply: 1)
  560. Cancer cells culture - (reply: 1)
  561. How to achieve uniform cell distribution in culture vessle - (reply: 6)
  562. viability/integrity stain of fungal cells - (reply: 5)
  563. Cell culture contamination - (reply: 3)
  564. airway primary cells stop growing at passage 3 - (reply: 1)
  565. cell culture neural cells easier? - (reply: 6)
  566. Question - Corticosterone, cell culture - (reply: 1)
  567. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  568. Where to obtain rat embryonic stem cells? - (reply: 3)
  569. Stable cell line - (reply: 1)
  570. PBMC cells handling - (reply: 1)
  571. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  572. Unable to clone in E. coli cells - (reply: 3)
  573. removing MHC class I from the cell surface - (reply: 3)
  574. Osteosarcoma cell culture problem - (reply: 7)
  575. rtPCR stem cell signalling array from quiagen- has anybody experience? - (reply: 1)
  576. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 4)
  577. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 1)
  578. colon cancer cell lines vs normal colon epithelial cells - (reply: 1)
  579. Cell count and seed, plz help... - (reply: 4)
  580. In and out of cell Help 2 - (reply: 6)
  581. In and out of cell Help - (reply: 2)
  582. Primary culture of breast tumor cells - (reply: 1)
  583. Strange Cell Morphology & Cell Debris Following Lentiviral Transduction - (reply: 2)
  584. Cell numbers versus confluency - (reply: 2)
  585. Flow cytometry in THP-1 cells - (reply: 1)
  586. Competent bacterial cells - (reply: 2)
  587. Could a change of FBS affect outcome of a cell proliferation assay? - (reply: 4)
  588. How do they make passage limited cells? - (reply: 1)
  589. transient transfection problem using ME180 cells - (reply: 1)
  590. How to completely remove cells from surface - (reply: 3)
  591. Hiperfect siRNA in HT-29 cells help - (reply: 2)
  592. Isolation of BOTH cells AND serums from mouse blood - (reply: 5)
  593. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  594. Commercial source Stomach Smooth Muscle Cell lysate - (reply: 1)
  595. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
  596. cell line contaminated by something... - (reply: 2)
  597. Flag Staining in U87 cells - (reply: 4)
  598. protein is not expessing in HEK 293 cells - (reply: 2)
  599. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
  600. HepG3 cell line origin - (reply: 3)
  601. dilution of cell suspension - (reply: 4)
  602. how to preserve spleen cells for long duration, for flowcytometry - (reply: 2)
  603. Increasing cell growth rate in plain M9 medium - (reply: 1)
  604. Puromycin - shRNA - stable cell line for knock down - (reply: 4)
  605. Reducing cell adhesion - (reply: 1)
  606. The Hek 293T cell line looks like non adherent cells after to be sorted in FACS - (reply: 1)
  607. Hygromycin selection of virally-transduced cell lines - (reply: 1)
  608. Which cells express which genes? - (reply: 1)
  609. IGF1 stimulation of THP1 cells - (reply: 2)
  610. Cells detach during bodipy 493/503 staining - (reply: 1)
  611. RNA isolation from small numbers of cells - (reply: 6)
  612. Distinguish murine and human cells by surface staining? - (reply: 1)
  613. starting HaCaT cell line. basic questions - (reply: 6)
  614. culture media for pancreatic cancer stem cells - (reply: 2)
  615. Lysis method for single human embryonic cell - (reply: 5)
  616. In vitro transcription/translated protein to cells!!! - (reply: 1)
  617. Cell plating - (reply: 2)
  618. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
  619. colony formation assay in A2780 cells - (reply: 4)
  620. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  621. Which is a better way to culture cells after thawing ? - (reply: 2)
  622. Comet assay sperm cells - (reply: 5)
  623. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  624. Live/Dead Staining of already Permeable Cells - (reply: 10)
  625. cell synchronisation for fibroblasts (help choosing method/chemical) - (reply: 2)
  626. Cell Therapies - (reply: 2)
  627. What is the activity of 1X Trypsin solutions? - (reply: 3)
  628. SURE E. coli competent cells - (reply: 1)
  629. Stable protein expression in Vero cells - (reply: 2)
  630. HL60 cell line suppliers - (reply: 1)
  631. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
  632. Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
  633. Question about HeLa cell overconfluence - (reply: 3)
  634. 293T cell contamination post-transfection - (reply: 1)
  635. HEK293T cells are too fragile?? - (reply: 2)
  636. number of splitting after defrozing cells - (reply: 2)
  637. my human iPS cells have a lot of differentiation - (reply: 4)
  638. Properly designing a drug treatment assay measuring cell cycle arrest - (reply: 2)
  639. Detaching Breast Cancer cells (MCF-10A, HCC70) without trypsin- used for FACs - (reply: 4)
  640. counting cells before adding them to 96 well plate - (reply: 7)
  641. Cell Suspension - (reply: 2)
  642. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 5)
  643. MDA-MB-231 cells undergoing senescence- help! - (reply: 2)
  644. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  645. coating cover-slides before seeding cells for fluorescent microscopy - (reply: 4)
  646. How to induce early apoptosis in cells without using chemicals or UV - (reply: 5)
  647. choice of cell line for orthotopic xenograft implant - (reply: 3)
  648. Compound concentration depending on cell number? - (reply: 4)
  649. How to remove mycoplasma contamination in cell lines? - (reply: 5)
  650. [Question] How to remove cell debris stuck on the attached cells in primary card - (reply: 2)
  651. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  652. Reagent used to adjust pH of cell culture media - (reply: 3)
  653. Powder indomethacin in cell culture media?? - (reply: 2)
  654. How many generation of HEK293 cell can undergo? - (reply: 1)
  655. [Question] how to make primary-cultured cells evenly distributed? - (reply: 11)
  656. small dots in cell culture - (reply: 2)
  657. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  658. Clumpy competent cells - (reply: 3)
  659. Preparation of bacterial cells for SEM and TEM - (reply: 2)
  660. G418 advice in estab;lishing stable cell line - (reply: 1)
  661. Electroporation of J774.2 murine macrophage cell line - (reply: 1)
  662. About trypsinizing adherent cells in 96-well plates - (reply: 2)
  663. Passaging cells - (reply: 12)
  664. Preparing homemade XL1-blue cells in CCMB80 buffer - (reply: 1)
  665. Understanding Cell Passaging - (reply: 5)
  666. How to extract DNA from small number of cells - (reply: 2)
  667. How to identify beta pancreatic cells in dish of heterogenous cells? - (reply: 12)
  668. Cell Splitting Ratio Question - (reply: 5)
  669. Sf9 cell suspension culture - (reply: 5)
  670. competent cell formation - (reply: 1)
  671. Help! Clontech Xfect is killing my T-Rex HeLa cells!!! Why! - (reply: 1)
  672. How long are cells viable on ice - (reply: 2)
  673. Discrepancy between tissue and cell culture - (reply: 1)
  674. What is the density of a human skin cell? - (reply: 8)
  675. Determining the % of GFP/CHERRY cells in co-mixed culture - (reply: 3)
  676. Is the genomic DNA similar among different cell lines? - (reply: 4)
  677. serumm free culture of adherent cho cells - (reply: 1)
  678. detection of mesenchymal stem cells in cancer tissue sample - (reply: 1)
  679. Can u suggest any myoepithelial cell isolation protocol with the help of reagent - (reply: 1)
  680. cell line cultured in suspension - (reply: 1)
  681. GFP cells in paraffin.....orange? - (reply: 1)
  682. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
  683. Cell staining for fluorometric assay - (reply: 5)
  684. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  685. Long-Term Culturing of SH-SY5Y Cells - (reply: 1)
  686. N acetyl cysteine solution for cell culture - (reply: 3)
  687. Aortic smooth muscle cell contamination - (reply: 1)
  688. need recommendation for stable transduction of fibroblast cells - (reply: 1)
  689. Sub G1 in cell cycle - (reply: 2)
  690. DMSO induces cluster growing in LNCaP cells? - (reply: 1)
  691. Cell cycle arrest - adherent cells going into suspension - (reply: 4)
  692. Prevent adherent cells from adhering - (reply: 3)
  693. INS-1E cells not adhere to the flask! - (reply: 1)
  694. A549 cells are not attaching to flask - (reply: 2)
  695. irradiated J774 cells - (reply: 1)
  696. Cell lysis with proteinase k - (reply: 2)
  697. One siRNA sequence, many cell lines - (reply: 1)
  698. Freezing cell lysates for westerns - (reply: 2)
  699. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
  700. adherence to host cell question - (reply: 2)
  701. New patch of sf9 cells not growing - (reply: 2)
  702. contamination in yeast cell culture - (reply: 8)
  703. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
  704. sf9 Insect cells questions - (reply: 3)
  705. Kit to mesure cell death - (reply: 4)
  706. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  707. J774 and Flow cytometry....are they really big cells ? - (reply: 9)
  708. immunostaining cells grown in matrigel - (reply: 1)
  709. Cell concentration for flow cytometry - (reply: 2)
  710. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
  711. Cells growing very slowly - (reply: 6)
  712. SH-SY5Y cells do not grow - (reply: 1)
  713. GH3 cells - (reply: 4)
  714. The connection between LPS and PMA stimulation for maturation of ThP1 cells - (reply: 2)
  715. Using normal competent cells in electroporation method? - (reply: 1)
  716. Washing n2a cells out of 75 cm flask? - (reply: 1)
  717. How to dissociate N2A cells after centrifugation? - (reply: 3)
  718. Undefinable particles in cell culture flask - (reply: 2)
  719. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  720. immunostaining of cells in culture - (reply: 1)
  721. healthy stem cells vs cancer stem cells - (reply: 1)
  722. values for mRNA molecules per cell - (reply: 1)
  723. Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
  724. MCF10A Tet-ON stable cell line generation - (reply: 1)
  725. Primary cell line - (reply: 2)
  726. Neurosphere Assay with neuroblastoma cell lines - (reply: 1)
  727. How to decontaminate adherent cells with gentamicin - (reply: 2)
  728. Contamination in CHO cell ? - (reply: 3)
  729. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
  730. How to purify RNA from a handful number of cells? - (reply: 5)
  731. Check cell viablity after FACS - (reply: 2)
  732. ISH on fluorescing cells - (reply: 1)
  733. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
  734. Protein extraction from dead cells - (reply: 1)
  735. Cells in suspension vs. plated - (reply: 2)
  736. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  737. Hepg2 cells growing on top of each other - (reply: 1)
  738. urgent! calculating concentration of compound with different cell amounts - (reply: 3)
  739. h9c2 cells culture - (reply: 5)
  740. Does anyone have experience of subjecting cultured cells to mechanical stress? - (reply: 1)
  741. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
  742. cell population doubling - (reply: 1)
  743. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
  744. Cell line licence - (reply: 1)
  745. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
  746. temperature tolerance of animal cells - (reply: 3)
  747. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  748. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
  749. Human insulin for cell culture? - (reply: 2)
  750. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  751. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  752. Trouble producing stable cell lines - (reply: 2)
  753. Infecting cells with virus - (reply: 1)
  754. Requesting basic protocols on cell sorting - (reply: 1)
  755. lysing mammalian cells in sds - (reply: 3)
  756. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
  757. Is it possible to assay the carbohydrate profile of a cell? - (reply: 2)
  758. changing cell culture media - (reply: 1)
  759. Preparing Palmitate:BSA solution for treatment of cells? - (reply: 7)
  760. trypsinization of adherent cells - (reply: 9)
  761. Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
  762. Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
  763. How to lysis cells for an RNA extraction - (reply: 1)
  764. Problem with THP-1 cell line - (reply: 3)
  765. normalization to protein content or cell number - (reply: 2)
  766. detection of phosphorylation from cell pellet - (reply: 2)
  767. are THP-1 Cells(Monocytes) or Macrophages resistant to Mycoplasma infection or - (reply: 4)
  768. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  769. Cell culture shared with yeast? - (reply: 2)
  770. E. coli cells refuse to grow! - (reply: 2)
  771. how to fraction stem cell at different cell cycle stage - (reply: 2)
  772. Making Nuclei From Mouse Cells - (reply: 1)
  773. Different cell lines different protein stability - (reply: 1)
  774. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
  775. Immunofluorescence Staining of Cells - (reply: 1)
  776. Nucleus fills the whole cell - (reply: 3)
  777. Generating stable cell line with linear DNA - (reply: 3)
  778. isolating cells from Cockroach - (reply: 2)
  779. Question about suspension cells - (reply: 7)
  780. Calu-3 cell line do not grow properly - (reply: 1)
  781. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
  782. Counting and plating cells - (reply: 3)
  783. cells die 4-6 days after thawing - (reply: 8)
  784. what is the trypsin X for subculturing - (reply: 1)
  785. Cell Envelope Proteins Smeared - (reply: 1)
  786. Calculating the amount of the desired cells in the medium ? - (reply: 3)
  787. long term storage of cells at -80 - (reply: 1)
  788. Trypan Blue Exclusion Test - (reply: 2)
  789. Making BL21 competent cells - (reply: 2)
  790. cell plating density - (reply: 2)
  791. What kind of cell culture contamination is it? - (reply: 8)
  792. Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
  793. xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
  794. to treat cells with inhibitor - (reply: 2)
  795. lysis buffer not lysing adherent cells? - (reply: 8)
  796. Trypan Blue Staining - (reply: 3)
  797. Culturing cells on a slide - (reply: 5)
  798. Culturing cells on a slide - (reply: 3)
  799. protocol for immunostaining primery cells - (reply: 5)
  800. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
  801. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
  802. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
  803. Could the use of different FBS affact my cells - (reply: 5)
  804. Analysis of high throughput screen for cell surface markers - (reply: 1)
  805. Lymphoblastoid cell culture - (reply: 9)
  806. SF9 Cells lose exponential growth, lack stationary phase - (reply: 3)
  807. Don't know why my cells are unhealthy - (reply: 2)
  808. Hybridoma cells not secreting antibody - (reply: 3)
  809. Determining gene of interest from Cell culture lines - (reply: 2)
  810. Three clones of a cell line? - (reply: 3)
  811. Freezing cells in DMSO straight into minus 80? - (reply: 4)
  812. MCF7 cells vitality - (reply: 3)
  813. Ultrasonic bath for cell lysis - (reply: 3)
  814. any methods to evaluate the attaching ability of suspension cells? - (reply: 6)
  815. Vascular Smooth Muscle cell Marker - (reply: 3)
  816. Foam in Cell Culture - (reply: 1)
  817. Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
  818. SURE cells - (reply: 1)
  819. DT40 Cell Synchronisation - (reply: 2)
  820. Bio-Rad Cell counter - (reply: 3)
  821. SKBR-3 cells dying - (reply: 3)
  822. New cell culture user with no teacher or trainer - (reply: 5)
  823. Help with some problems with Culturing Epithelial Cells - (reply: 5)
  824. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
  825. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
  826. What are these large multinucleated cells??? - (reply: 1)
  827. Searching for a live cell staining method - (reply: 1)
  828. WT BRAF/NRAS mouse Melanoma cells - (reply: 2)
  829. HEK 293 cells Detachement - (reply: 8)
  830. Cell culture in candle jar - (reply: 2)
  831. centrifugation speed/cell prep for flow - (reply: 4)
  832. Huge background with HT22 cells phophoERK antibody - (reply: 3)
  833. Huge background with HT22 cells phophoERK antibody - (reply: 1)
  834. Competency cells analysis - (reply: 1)
  835. How many cells needed for co-IP and mass spec - (reply: 4)
  836. Performing MTT assay in suspension cells (new in the subject) - (reply: 7)
  837. cells die after transfection - (reply: 1)
  838. Seeding cells on small metal screws - (reply: 1)
  839. Cell lysate turbidity upon long storage - (reply: 3)
  840. Host cells for transformation - (reply: 3)
  841. Splitting or feeding cells for the weekend - (reply: 1)
  842. Black moving particles in the cell Culture - (reply: 2)
  843. lymphocyte proliferation using trypan blue - (reply: 2)
  844. cell culture - (reply: 1)
  845. cells shrink, detach from each other when changing media - (reply: 3)
  846. Newbie help: Seeding cells to 6-well plates - (reply: 2)
  847. 3t3 cell tured black - (reply: 9)
  848. calculating the number of cells to freeze down - (reply: 2)
  849. Fixed cells, sticky cells? - (reply: 2)
  850. What is cotransfection in molecular cell biology? - (reply: 1)
  851. BSA in adipocyte cellculture? Alternatives?? - (reply: 1)
  852. serum deprivation of cell lines - (reply: 1)
  853. serum deprivation of cell lines - (reply: 1)
  854. What to do Post-transfection of cells with siRNA? - (reply: 2)
  855. Problem with HEK stable cell line - (reply: 1)
  856. HEK 293 cell culture - (reply: 3)
  857. HepG2 cells resistant to Trypsinization - Please help!! - (reply: 8)
  858. difficulty in passaging HK-2 cells - (reply: 5)
  859. difficulty in passaging HK-2 cells - (reply: 2)
  860. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  861. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
  862. More cells in an antibiotic plate than in a plate without antibiotic???? - (reply: 2)
  863. what are these cells?? - (reply: 1)
  864. How to choose the right plasmids for certain types of cell? - (reply: 1)
  865. Isolation of rat endothelial cells - (reply: 2)
  866. protocol for making e.coli competent cells under low temperature - (reply: 1)
  867. Looking for cell surface markers. - (reply: 3)
  868. common nuclear marker for T cells - (reply: 1)
  869. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
  870. Any reliable markers for mouse memoery T cells? - (reply: 1)
  871. Do mouse endothelial cells express the alphaVbeta3 integrin? - (reply: 1)
  872. Cell Culture debris or contamination? - (reply: 4)
  873. primary cell isolation from CNS, how to remove myeline debris - (reply: 1)
  874. Platinum-A cell trasnfection toruble - (reply: 1)
  875. cell culture media refrigeration - (reply: 4)
  876. abnormal epithelial cells in mouse mammary gland - (reply: 1)
  877. cell DNA content of cells grown on different scaffolds - (reply: 2)
  878. Cancer cell starvation - (reply: 1)
  879. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
  880. Housekeeping gene for COS-1 cells - (reply: 1)
  881. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
  882. Monocytes die after cell scraping - (reply: 2)
  883. The water pan got dry in the TC incubator. Will my cells be OK? - (reply: 1)
  884. Cell fractionation - (reply: 2)
  885. Media formulations for MDA-MB-231, BT-474 cells and Caco-2 cells - (reply: 1)
  886. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
  887. Dropped cells into liquid nitrogen tank - (reply: 3)
  888. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
  889. Small intestinal cell line for transport studies - (reply: 4)
  890. help needed in determining volume of cells to be seeded - (reply: 6)
  891. Dilution, Cell concentration & DNA content - (reply: 3)
  892. competent cells - (reply: 2)
  893. Cell lines & gene of interest - (reply: 1)
  894. Software for selection genes related to concrete cell processes - (reply: 1)
  895. Immunocytochemistry with HEK293 cells - (reply: 1)
  896. How to determine apoptotic cell types in-vivo? - (reply: 1)
  897. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
  898. data analysis for single cell size and distribution - (reply: 1)
  899. determining optimal cell count for MTT - (reply: 2)
  900. Extracting DNA from FACS sorted cells - (reply: 3)
  901. Storing frozen cells in -80C instead of liquid nitrogen - (reply: 7)
  902. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
  903. DG44 CHO cells and Methotrexate - (reply: 1)
  904. extract plasmids from transfected eukaryotic cells - (reply: 6)
  905. Trypsin Deactivation - (reply: 4)
  906. Staining Cells in Suspension - (reply: 4)
  907. cell culture confusion - (reply: 1)
  908. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  909. chambered cell culture-slide for confocal microscopy - (reply: 3)
  910. Nicotiana tabacum (BY-2) Suspension cells contaminated! Help! - (reply: 1)
  911. urgh...mycoplasma drama..... - (reply: 5)
  912. Dead or Alive... -_- (MCF-7 cells) - (reply: 7)
  913. how to wash cells after fixation for a live binding study - (reply: 1)
  914. Evenly distribute 100ul of cells in a 48/96 well - (reply: 2)
  915. Cell lysis before shearing - (reply: 5)
  916. really low puromycin concentration for selection of cells - (reply: 6)
  917. Single stain control for 293 cell line - (reply: 4)
  918. Is mCherry denatured by Trypsin or RIPA buffer? - (reply: 7)
  919. loading dendritic cell with antigen - (reply: 1)
  920. Cell Lysis and Western Blotting - (reply: 1)
  921. problem with embryo carcinoma cell line , p19 , - (reply: 1)
  922. In vitro induction of apoptosis in tumor cells by inactivated NDV and IAV - (reply: 1)
  923. problem with embryo carcinoma cell line , p19 , - (reply: 1)
  924. Recombinant protein from cell lysate - (reply: 4)
  925. How to test if a compound is entering cells? - (reply: 3)
  926. help with cell seeding - (reply: 2)
  927. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  928. RNA isolation from frozen cells - (reply: 2)
  929. How do you coat coverslips before seeding cells? - (reply: 3)
  930. Buy.Ask for buying some cell lines and antibodies - (reply: 3)
  931. competentce cell, cloning - (reply: 2)
  932. Flp-recombinase target site integration in 293 T-REx cells - (reply: 1)
  933. Storage of transfected PC12 cells - (reply: 1)
  934. cell adhension - (reply: 1)
  935. Using Methanol For Cell Lysis - (reply: 1)
  936. Adjusting pH of cell culture medium for ELISA - (reply: 1)
  937. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
  938. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
  939. Contamination of primary cells with fibroblast. - (reply: 1)
  940. Help With Lysing HEK293 & Fibroblast Cells - (reply: 1)
  941. problem with siRNA transfection of HEK cells - (reply: 5)
  942. Splitting A549 cells - (reply: 9)
  943. NMDA Transfection in 293 Cells - (reply: 1)
  944. freezing adherant cells - (reply: 2)
  945. freezing adherant cells - (reply: 2)
  946. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
  947. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
  948. cells stably expressing T7 RNA polymerase - (reply: 1)
  949. Cell Fixation - Experience with cell fixation? (reply: 5)
  950. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  951. Is pCL-Eco suitable for packaging viruses that used to infect human cells? - (reply: 1)
  952. Cause of Death for Cell Culture? - (reply: 5)
  953. creat a SY5Y stable cell line - (reply: 1)
  954. selecting drug-resistant colon cancer cells - (reply: 2)
  955. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
  956. Helper T cell skewing - (reply: 3)
  957. Optimum serum starvation period for NIH, CHO, COS7 cells - (reply: 4)
  958. Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
  959. competent cells stock - (reply: 3)
  960. intracellular cytokine staining - re-stimulation of activated T cells (reply: 1)
  961. DH5alpha cells - (reply: 4)
  962. pheonix cells and &293A cells - (reply: 1)
  963. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
  964. Protein purification from HEK 293 cells - (reply: 1)
  965. Growing hybridoma cells in medium without serum - (reply: 2)
  966. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
  967. Cell drawing tools? - (reply: 6)
  968. How many cells in plant embryos ? - seed biology (reply: 1)
  969. cell count - (reply: 8)
  970. question on making chemical and electrocompetent cells - (reply: 2)
  971. COS7 cells giving me problems - (reply: 1)
  972. Where to get 5T series cells? - (reply: 1)
  973. cell number for colony formation - HEK293, NIH3T3 (reply: 1)
  974. Electroporation/electrotransfection mammalian cells - (reply: 7)
  975. Cell debris here, there, everywhere!? - (reply: 2)
  976. Cell debris here, there, everywhere!? - (reply: 1)
  977. Mini prep of frozen bacteria cells - (reply: 2)
  978. Help! Turned on UV light. Did I kill my cells? - (reply: 2)
  979. growing competent cells - (reply: 1)
  980. Freezing density for NIH 3T3 cells - (reply: 3)
  981. Stable Cell Line generation - (reply: 3)
  982. making more competent cells - (reply: 2)
  983. CellTracker dye in necrotic cells? - (reply: 1)
  984. The case of the punky Sf9 cells - (reply: 2)
  985. sticky perm cells (tween 20)- can I prevent it? - (reply: 2)
  986. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
  987. collecting cells HT1080 - (reply: 4)
  988. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
  989. Help: problem with MDCK cells - (reply: 2)
  990. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
  991. Oh my cell culture problems never cease!!! - (reply: 4)
  992. WEHI-3BD cell culturing - Advice on culturing. (reply: 5)
  993. cell homogenization - (reply: 13)
  994. cell count - (reply: 3)
  995. cell lysis - confocal slides (reply: 4)
  996. HeLa cell anatomy question - (reply: 3)
  997. Need cell lines wich express separately TLR 2, 4 and 5 - (reply: 1)
  998. MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
  999. THP-1 cell line - (reply: 3)
  1000. CaMKII alpha promoter and cell tranfection - (reply: 1)
  1001. Adaptation of mammalian cells to serum free mode - (reply: 2)
  1002. Live cell Imaging medium - (reply: 1)
  1003. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  1004. MDA-MB-231 cell transfection - (reply: 5)
  1005. Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
  1006. Break Cell Clumps - prepare single cells for injections (reply: 1)
  1007. Stem Cells Sphere - stem cells (reply: 2)
  1008. Request for cell lines? - (reply: 1)
  1009. Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
  1010. MTT problems with Huh7 cell line, plzzzzzzz help - MTT color problem (reply: 5)
  1011. Calculating cell doubling time - (reply: 1)
  1012. SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
  1013. Infected cells that are dying after puromycin selection - (reply: 1)
  1014. contamination issue in cell culture - (reply: 1)
  1015. Why do cells need to be washed in PBS before biotinylation? - (reply: 3)
  1016. AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD (reply: 2)
  1017. Purification of recombinant proteins from insect cells - (reply: 2)
  1018. Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
  1019. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
  1020. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
  1021. Growth curve for PC3 cell line - (reply: 1)
  1022. Agrobacterium EHA101 competent cells - (reply: 1)
  1023. THP-1 cell line - (reply: 2)
  1024. Effective DCFDA Protocol for Non-Adherent THP-1 Cells? - (reply: 1)
  1025. Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
  1026. best freezing method for electrocompetent cells - (reply: 9)
  1027. cell seeding calculation - (reply: 8)
  1028. Improved Stem Cell Proliferation - Would interested in improving proliferation of adult stem cells? (reply: 5)
  1029. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  1030. Yeast contamination in cell culture - (reply: 8)
  1031. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
  1032. Remove cells from dish and fix them in slides - (reply: 3)
  1033. Help- lysing cells with triton x-100 - (reply: 1)
  1034. E.coli cells lyse in minimal media - (reply: 3)
  1035. How stable are suspension cells in room temperature? - (reply: 2)
  1036. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
  1037. Transfection in insect cell lines?? - (reply: 1)
  1038. PCR genomic DNA using cell lysate - (reply: 1)
  1039. HELA Cells Capable of producing whole, infective HPV particles? - (reply: 3)
  1040. Distilled water for Electrocompetent Cell Preparation - (reply: 6)
  1041. Should I fix cells digested from tissues? - (reply: 4)
  1042. STR cell line identification - looking for cell line identification method (reply: 1)
  1043. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
  1044. Rat Dental Pulp Stem Cell Culture - (reply: 1)
  1045. NK cell analysis using CD16PE and CD56PE - (reply: 2)
  1046. My neuroblastoma are floating!! please help - Both SK-NSH and SH-SY5Y are adherent cells, but are only floating now (reply: 3)
  1047. cell suspencion and medium - (reply: 5)
  1048. One Shot TOP10 Cells - (reply: 6)
  1049. trypan blue for chlorella - (reply: 1)
  1050. Help on BmN4 cell culture - How to maintain BmN4 cells? (reply: 1)
  1051. Compare ChIP to cell lysate - (reply: 1)
  1052. Compare ChIP to cell lysate - (reply: 1)
  1053. Testing cytoxixity on healthy human cells - (reply: 2)
  1054. what cell line to use - to demonstrate ubiquitination (reply: 1)
  1055. GFP cells intracellular staining - (reply: 1)
  1056. Xenograph - how to maintain cell viability? - (reply: 1)
  1057. Western blot of apoptotic cells - (reply: 2)
  1058. cell sycle and proliferation - (reply: 3)
  1059. help with cell dilution - (reply: 2)
  1060. Difficult Cell Proliferation Experiment - (reply: 2)
  1061. Cell dilution cloning - Cells cloning (reply: 2)
  1062. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
  1063. HTC116 cells detaching !!!! - (reply: 2)
  1064. Revival of kasumi-1 cells - (reply: 3)
  1065. HEK 293 Trypsin vs. only EDTA - (reply: 2)
  1066. Growth conditions for E. coli DB3.1 cells - (reply: 1)
  1067. Mycoplasma cleanup time... - (reply: 10)
  1068. weird & curious problem with CHO cell culture - (reply: 3)
  1069. HaCat do not grow after using cell scraper - (reply: 5)
  1070. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
  1071. Cell Lysates from a Western Blog - (reply: 2)
  1072. Loss of YFP signal in transduced T cells - (reply: 7)
  1073. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
  1074. Freezing cell pellets and minimizing lysis - (reply: 2)
  1075. Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells? (reply: 6)
  1076. Total Cell protein - (reply: 1)
  1077. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
  1078. Mycoplasma - (reply: 1)
  1079. cell culture media - glutamine! - (reply: 2)
  1080. How many cells do you have in a confluent T 75 flask? - I have 1/2 million (reply: 2)
  1081. how to make antigen by trypsin and papain digetion - plz teel me hoe to make antigen by trypsin & papain digetion (reply: 1)
  1082. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
  1083. Premature cell lysis - (reply: 2)
  1084. urgent!!! weird Growth in cell line - (reply: 8)
  1085. Shipping of blood cells over long distance - (reply: 1)
  1086. refrozen cell pellet - (reply: 1)
  1087. Drug vehicle giving toxicity in cell culture - With increse in vehicle..... toxicity decrese (reply: 4)
  1088. contamination in cell line...... - (reply: 4)
  1089. Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 7)
  1090. Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
  1091. MTT with cells growhing in suspension, not adherent - (reply: 3)
  1092. Cell ELISA - No differences between cells expressing and not, the receptor (reply: 2)
  1093. growth curve for attached cells - (reply: 1)
  1094. cells dont attach hard enough to 96 well - (reply: 3)
  1095. CTLL-2 culture - preventing cell clumping - (reply: 2)
  1096. Yet another contamination question - SF9 cells (reply: 11)
  1097. Problems with transfection in human cells - (reply: 3)
  1098. HepG2 cells culture conditions - (reply: 2)
  1099. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
  1100. T cell proliferation problem - (reply: 3)
  1101. How to Handle Cells Shipped Internationally in Dry Ice? - (reply: 2)
  1102. Differences between cells in different species - (reply: 5)
  1103. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
  1104. isotype control for GFP expressing cells? - (reply: 2)
  1105. Trypsin for J774A Macrophages? - (reply: 2)
  1106. Trouble with growth of transformed cells - (reply: 4)
  1107. nuclear extraction of blood cells - (reply: 2)
  1108. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  1109. ELISA with undiluted cell lysate samples - (reply: 1)
  1110. How can you expand cells if you have one dish of them? - (reply: 1)
  1111. Androgen independent LNCaP cells - (reply: 2)
  1112. mycoplasma detection kit - (reply: 1)
  1113. What is the proper way to infect cell culture with virus? - (reply: 5)
  1114. Micro-RNA profiling for differentiating cells - (reply: 1)
  1115. Growing insect cells with other cell lines - (reply: 2)
  1116. Candida albicans cells lysis - method (reply: 1)
  1117. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
  1118. Advise for Whole Cell Stain - hESC-differentiated Trophoblast (reply: 1)
  1119. 293 T cells - (reply: 3)
  1120. Mouse Bone Marrow Isolation - How to increase yield of BM-derived cells? (reply: 1)
  1121. BL-21 Gold (DE3) cells and further strains - (reply: 2)
  1122. Cell Cycle Analysis flow cytometry - How to measure desynchronisation rate of cells (reply: 1)
  1123. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
  1124. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
  1125. Fixing cells before staining of surface markers - (reply: 1)
  1126. Black dots in cell culture - (reply: 7)
  1127. cell counting - (reply: 5)
  1128. Optimization for cell viability assay (MTT assay) - (reply: 1)
  1129. Anyone thought about reprogram fibroblast to liver cells? - (reply: 2)
  1130. cell confluence in cell cultures - myoblast c2c12 in particular (reply: 2)
  1131. cell line adherence - cells adhering to flask very tightly after transfectiono (reply: 1)
  1132. Cell labelling - How can I label 2 cell populations? (no transgenes) (reply: 6)
  1133. How long does it take for cells to reattach? - From balls to pancakes. (reply: 3)
  1134. Dot plot cell cycle analysis-strange dots - (reply: 4)
  1135. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
  1136. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  1137. help Splashed Raji cells on skin/face/hand - (reply: 2)
  1138. Help! ES Lenti infection efficiency=nightmare - trouble getting over 40 % cells infected (reply: 8)
  1139. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  1140. CO2 level for cell culture - (reply: 6)
  1141. sphere and suspension culture subculturing - (reply: 1)
  1142. suspension cells for confocal - (reply: 1)
  1143. RNAi is somatic and germline cells - (reply: 3)
  1144. Luciferase and cell responsiveness - (reply: 3)
  1145. adult cell culture - (reply: 1)
  1146. protein overexpression in HeLa cells - (reply: 1)
  1147. Insulin supplements to cell culture media - (reply: 1)
  1148. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
  1149. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
  1150. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
  1151. topic and cell culture - (reply: 2)
  1152. Running a western with cell media in gels? - (reply: 11)
  1153. Starting cells for making one's own competent cells - (reply: 7)
  1154. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  1155. Persistent stable cell line (Bos23) Contamination... - (reply: 2)
  1156. Hematopoietic cells separation - How to get high purity and viability hematopoietic cell populations? (reply: 2)
  1157. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  1158. min6 cells immunofourescence staining - staining and culture problem (reply: 4)
  1159. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
  1160. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  1161. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
  1162. rat ES cells - (reply: 2)
  1163. Mycoplasma contamination - (reply: 1)
  1164. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
  1165. establishment of primary cell line from biopsy - (reply: 2)
  1166. Cell culture fasting - (reply: 1)
  1167. mutated HeLa cells? - from cycloheximide? (reply: 1)
  1168. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
  1169. qPCR for transformed cells - (reply: 1)
  1170. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
  1171. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
  1172. descrepancy between total cell lysate and cell fractionation - (reply: 1)
  1173. Disruption of cells using mild detergent - (reply: 2)
  1174. Eruption of mammalian cells using detergent - (reply: 1)
  1175. lenti virus transfected cell dies... - (reply: 4)
  1176. Extremely Weird Issue - CHO Cell Culture (reply: 2)
  1177. Cell Die.. Need help - (reply: 1)
  1178. Cell scraping ruining my life - (reply: 6)
  1179. How much trypsin/Cause of death - (reply: 3)
  1180. Death of cells in the control. - (reply: 1)
  1181. cell culture clarification - I need help please (reply: 2)
  1182. Serum concentration in treatment of cells for MTT assay - (reply: 1)
  1183. Minimum number of cells needed for cytometry? - (reply: 3)
  1184. Beta-Lactam in CHO Cells - (reply: 1)
  1185. problems with 293T cells after medium removal!!! - (reply: 6)
  1186. B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
  1187. Propagating ccdB survival cells - (reply: 6)
  1188. Western difficulty in primary neuron cells. - (reply: 1)
  1189. DOubt - Competent cell (reply: 2)
  1190. Differentiation of CD11b cells into macrophages.Help please - (reply: 1)
  1191. Please Help!! - Mycoplasma (reply: 5)
  1192. Primary keratinocyte isolation- cell loss - (reply: 1)
  1193. cells attach but don't flatten out - MDA-231 (reply: 8)
  1194. Problem Generating Stable HeLa Cells - (reply: 1)
  1195. Retroviral transduction of T cells - (reply: 4)
  1196. Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
  1197. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
  1198. Multiple nuclei?? - cell morphology - FaDu (reply: 8)
  1199. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  1200. how to compensate the cell death during transfection by electroporation - (reply: 2)
  1201. H-ras transformed RK3E/tv-a cells - (reply: 1)
  1202. HEK001 Cell Line and Papillomavirus - Does HEK001 cell line produce a functional papillomavirus (reply: 4)
  1203. 2 questions: myeloma cell lines and mice MABs - (reply: 5)
  1204. I'm getting fried-egg looking cells... What should I do?? - (reply: 8)
  1205. Empty bubbles in the 3T3-L1 cells - (reply: 3)
  1206. Exposing Cell Lines to Drugs Metabolized by Liver Microsomes - (reply: 1)
  1207. UV safety in cell culture hood - (reply: 3)
  1208. Where can I find info on the physical appearance of TB cell wall structures? - I am an artist wanting to depict the cell wall with absolute accuracy. (reply: 7)
  1209. intracelluar staining without killing cells - (reply: 3)
  1210. Info about A7r5 cell culture - (reply: 1)
  1211. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  1212. Cell lysates - Protein precipitation? - (reply: 3)
  1213. Glucose in epithelial cell - (reply: 1)
  1214. cell lysate for pulldown - (reply: 3)
  1215. cell culture media - (reply: 3)
  1216. Competent cell storage for long term use - (reply: 2)
  1217. My RIPA cell lysis buffer - Western blot (reply: 3)
  1218. Cell Uptake Study - (reply: 1)
  1219. what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
  1220. aspects of RAW264.7 cells - (reply: 5)
  1221. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
  1222. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
  1223. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
  1224. Hoechst Stain does it stain dead cells - (reply: 3)
  1225. Mycoplasma detection - (reply: 2)
  1226. Help for cell preparation for FACS... - (reply: 1)
  1227. Cell Lysis Buffer - (reply: 6)
  1228. HeLa cells and HPV - (reply: 2)
  1229. Resolving cell growth problems using growth additives - way to resolve urgent cell growth problems (reply: 3)
  1230. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  1231. problem with MACS cell isolation - (reply: 5)
  1232. cell culture split ratios. - (reply: 2)
  1233. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
  1234. detection of mycoplasma with hoechst staining - (reply: 1)
  1235. blood red cells - (reply: 1)
  1236. Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
  1237. NFKb signalling in cell line ??? - (reply: 2)
  1238. kit fpr cell membrane preparation? - (reply: 2)
  1239. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
  1240. How to find NFKb signalling is active in a cell line??? - (reply: 2)
  1241. Fixing YFP cells - (reply: 2)
  1242. MTT Assay: cell proliferation or cell viability??? - (reply: 4)
  1243. Rescue RAW cell culture - RAW cells past confluent point... can they be rescued? (reply: 4)
  1244. cell cycle arrest by colcemid - (reply: 2)
  1245. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
  1246. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
  1247. PROLIFERATION ASSAY IN MICROTITER PLATES WITHOUT A SEMIAUTOMATIC CELL HARVESTER - (reply: 1)
  1248. THP cells? what does THP stands for?????????? - (reply: 1)
  1249. Immunioprecipitation from Jurkat cells?? - (reply: 1)
  1250. Weird effect of siRNA on protein in cells - (reply: 3)
  1251. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  1252. EDTA addition to trypsin solution (1x) - (reply: 1)
  1253. Apoptosis induction - for vero and mcf-7 cells (reply: 5)
  1254. Need pH 6 Cell culture media - (reply: 1)
  1255. Are my cells undergoing epigenetic changes? - (reply: 2)
  1256. establishment of stable cell line using hek293t - (reply: 2)
  1257. Cell pellet frozen -80 then -20 - (reply: 4)
  1258. total cell protein vs membrane protein - (reply: 4)
  1259. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  1260. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
  1261. Fixing the cells for FACS - Paraformaldehyde or Formaldehyde (reply: 2)
  1262. Problem with control samples in cell IC - (reply: 3)
  1263. Recommendation for cell cycle analysis software needed - (reply: 1)
  1264. Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
  1265. Cell culture contamination question - what the hell are these things?? - (reply: 3)
  1266. 'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
  1267. Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
  1268. Immunofluorescence using dead cells? - (reply: 1)
  1269. floating THP-1 cells - medium change advice - (reply: 2)
  1270. my cells love infection, any thoughts on antibiotics? - (reply: 3)
  1271. Culture Media for stem cells - (reply: 1)
  1272. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
  1273. Anyone heard of cells spontaneously fluorescing red?? - (reply: 4)
  1274. Freezing cells - How long can I keep cells at -80C? (reply: 6)
  1275. Help regarding cell line - (reply: 1)
  1276. organizing cell lines - (reply: 1)
  1277. Cell plating density - (reply: 3)
  1278. Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
  1279. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
  1280. MDA-MB-231 cells question - (reply: 3)
  1281. thawed cells wont adhere - pc3 and LNcaP (reply: 2)
  1282. DiFi Cell line (colon carcinome) - Where can I get it???? (reply: 1)
  1283. HaCaT cells - (reply: 2)
  1284. lncap and MCF-7become ethanol sensitive? - are lncap cells and MCF-7 cells ethanol-sensitive? (reply: 2)
  1285. Dying endometrial stromal cells - (reply: 1)
  1286. Help me - Are my cells infected with something or could this be unsusual morphol - (reply: 5)
  1287. how to dissociate cells for cell migration assay - (reply: 3)
  1288. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
  1289. RNA extraction - how many cells do I need at least?? (reply: 4)
  1290. RNA extraction protocol for small amounts of cells - (reply: 2)
  1291. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
  1292. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
  1293. strange morphology of sp2/0 cells after fusion!!! Please help! - morphology switch from round to triangular!!!! (reply: 2)
  1294. fabrablast primery cell line problem - how to detach cell well (reply: 1)
  1295. what solvent is better compatible for most cell lines? - DMSO vs methanol (reply: 3)
  1296. Cell growth rate & O2 consumption - (reply: 2)
  1297. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
  1298. Cells stick to dish in Embryoid Bodies - (reply: 1)
  1299. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  1300. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
  1301. how to plate cells precisely - (reply: 1)
  1302. RNA extraction from stained cells - (reply: 2)
  1303. Methods to assess cell viability - (reply: 1)
  1304. suspension cell transient transfection - (reply: 10)
  1305. Adherent cells washing off in 96 well -MTT - (reply: 4)
  1306. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  1307. plasmids - plasmids for stable cell line construction (reply: 2)
  1308. abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
  1309. average total protein content per 293t cell - (reply: 2)
  1310. T cells preparation for FACS - (reply: 1)
  1311. counting cells - (reply: 3)
  1312. Cell OD and spec problems - (reply: 1)
  1313. cell confluency & effect on cells...?? - (reply: 4)
  1314. Pipet trypsin tips - (reply: 3)
  1315. Fixing cells for flow cytometry - alternative to 1% paraformaldehyde (reply: 3)
  1316. mycoplasma false positive - Luminescence false positives for myco (reply: 2)
  1317. Washing Red Blood Cells - Tips on washing red blood cells (reply: 8)
  1318. MEF Cell marker - (reply: 1)
  1319. (CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
  1320. Change of colour in cell culture media...? - (reply: 2)
  1321. cell count - how to calculate (reply: 2)
  1322. generating cell line knockouts - (reply: 3)
  1323. Cancer Stem Cells?? - (reply: 10)
  1324. Some kind of contamination? - mouse transduced cell line (reply: 7)
  1325. Cell stimulation of transcription - (reply: 5)
  1326. Mouse blood density/osmolarity of blood cells for separation - (reply: 5)
  1327. suitable cell lysis buffer - (reply: 1)
  1328. Toluidine blue staining for cell culture - (reply: 1)
  1329. cell culture - 3D cell culture (reply: 1)
  1330. Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
  1331. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  1332. MEF culture contamination? - Contaminated cell culture? (reply: 2)
  1333. very simple question about hemocytometer cell counting - (reply: 1)
  1334. nucleic acid extraction from silenced cells - (reply: 6)
  1335. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
  1336. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
  1337. In Vitro Drug Exposure Cytotoxicity Testing and Cell Confluency - (reply: 8)
  1338. Dendritic cells - Protocol of stimulation of dendritic cells (reply: 3)
  1339. giant cells in HEK293 cell line - (reply: 10)
  1340. Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
  1341. Plant cell transfection - (reply: 1)
  1342. stable cell line - selection after retroviral infection (reply: 1)
  1343. Storing cell lines long-term at -70C - (reply: 4)
  1344. Commercially available primary mouse endothelial cell line? - (reply: 2)
  1345. Frozen cells in -80 - (reply: 1)
  1346. Plant or Fungal Cell Dissociation Technique? - (reply: 3)
  1347. how can i bring cells onto a slide? - ...for microscopical observation! (reply: 7)
  1348. cell density to be seeded? - cytotoxicity studies (reply: 1)
  1349. Lysis of the cells - (reply: 1)
  1350. Cells do not lyse in SDS buffer - (reply: 1)
  1351. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  1352. lysis buffer for lymphoblast cells - (reply: 1)
  1353. problem with cell cycle analysis - (reply: 3)
  1354. THP-1 Cells differentiation - THP-1 cells differentiated using IFN-gamma/LPS (reply: 7)
  1355. Single cell suspension from solid tumor - (reply: 3)
  1356. cell aggregation? - (reply: 1)
  1357. T-Cell receptor - Is it a single type on a T cell or multiple types? (reply: 1)
  1358. cells weak adhesion - detachment (reply: 4)
  1359. how we count the passages in cell cultures? - (reply: 5)
  1360. resolving cell growth problems - (reply: 2)
  1361. clumping of cells - (reply: 7)
  1362. stability of cell suspension - (reply: 1)
  1363. cell adhesion in serum free condition - (reply: 1)
  1364. effects of cell confluency - (reply: 1)
  1365. Which trypsin EDTA to use? - (reply: 3)
  1366. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
  1367. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  1368. polarized cells - (reply: 4)
  1369. PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
  1370. cell adhesion assay - using collagen (reply: 1)
  1371. Problem clariflying/filtering insect cell lysate - (reply: 2)
  1372. cell culture urgent!! - (reply: 2)
  1373. Moving S2 cells from one plate to another - (reply: 1)
  1374. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
  1375. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
  1376. mononuclear cells medium - I am stuck with the concentration of 2-mercaptoethanol (reply: 1)
  1377. Accidental thawing of frozen cells - (reply: 3)
  1378. how to start with a new cell line ? - how to start with a new cell line ? (reply: 1)
  1379. HaCaT cells die after trypsinization - (reply: 1)
  1380. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
  1381. GFP and Luciferase Cell Line - (reply: 2)
  1382. Transfecting primary cells with plasmid - (reply: 1)
  1383. transfecting and selecting EL4 cells - (reply: 1)
  1384. Induce Osteoblast lineage from C2C12 myoblast cells - (reply: 1)
  1385. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
  1386. Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
  1387. Transfection into eukaryotic cells - (reply: 1)
  1388. Fixing cells with PFA - (reply: 1)
  1389. A549 cells not doubling - has anyone been through this with other cell lines? (reply: 4)
  1390. Contamination in SH-SY5Y cultures, mycoplasma? - (reply: 2)
  1391. Immunofluorescence in HEK cells issues - (reply: 1)
  1392. cell sorting - losing cells? - (reply: 3)
  1393. What if my expressed protein is toxic for the cells... - (reply: 1)
  1394. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  1395. Possible contamination of leukemic cell culture - debris, growth inhibition, cell aggregates (reply: 3)
  1396. human cancer cell line splashed into my eyes - (reply: 3)
  1397. Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
  1398. BL21(DE3)LysS competent cells - (reply: 1)
  1399. ATP turnover in neuron - ATP hydrolysis rates in neuronal cells (reply: 2)
  1400. Adhering CHO cell culture - cells don't adhere! (reply: 2)
  1401. do e.coli cells get ruptured during high spin - (reply: 1)
  1402. Please Help with COS-1 cells^^ - (reply: 1)
  1403. antibiotics for preventation of mycoplasma contamination - (reply: 4)
  1404. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
  1405. cell counting - to get an impression when my cells are in the log phase (reply: 1)
  1406. Whole cell lysis of fibroblasts - (reply: 1)
  1407. Whole cell lysis of fibroblasts - (reply: 1)
  1408. test of cytotoxic effect of compounds - why cells are pretreated with actinomycin C ?? (reply: 1)
  1409. Proliferation Assay - Should cells be in the logarithmic phase ? (reply: 3)
  1410. cell density and growth - (reply: 5)
  1411. Laser capture microdissection - capturing single cells (reply: 2)
  1412. when cells have to be synchronized?? - (reply: 2)
  1413. cells in RNAlater - the title say`s it all :) (reply: 4)
  1414. Cytotoxicity assay for adherent cells exposed to particles - (reply: 1)
  1415. Murine Cell Lines - (reply: 2)
  1416. Cell Proliferation: To adhere or not to adhere (before treatment), that is the q - (reply: 2)
  1417. why are some cell lines more popular than others?? - (reply: 4)
  1418. NK cell staining - (reply: 4)
  1419. cell culture media - with or w/o L-glutamine? (reply: 2)
  1420. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  1421. pro-apoptotic effect of compounds on cancer cell lines - which cell line to test the non-toxic effect? (reply: 3)
  1422. Expiry of Trypan blue - (reply: 3)
  1423. Question in Generating double stable cell line - (reply: 3)
  1424. activity of sex-chromosome in somatic cells - (reply: 5)
  1425. CaCl2 for making competent cells - (reply: 3)
  1426. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
  1427. ICC Cell Adherence Issue - (reply: 1)
  1428. gentamycin resistance in human cencer cell culture - please suggest the alternative antibiotic (reply: 2)
  1429. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
  1430. How to normalize my samples before I transfect my cells - (reply: 6)
  1431. Isolation of intestinal epithelial cells - Isolation of intestinal epithelial cells (reply: 2)
  1432. Number of proteins expressed in cell - (reply: 2)
  1433. Do HEK 293 cells contain functional E1A/E1B? - (reply: 2)
  1434. Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
  1435. Calcium phosphate method vs lipofectamine - troublemaker neuroblastoma cells (reply: 7)
  1436. Which cell lines are best for transient transgene expression? - (reply: 3)
  1437. Alzheimer's cell model - (reply: 7)
  1438. Partial cell subculture? - (reply: 2)
  1439. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  1440. Whole Kidney primary cell culture - (reply: 1)
  1441. dh5 alpha competent cells? - (reply: 2)
  1442. which is the best lysis buffer for whole cell extracts? - (reply: 1)
  1443. Please help me to understand- cell counting - (reply: 1)
  1444. Stimulating hepatocytes with IL-1beta - (reply: 1)
  1445. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  1446. Cell count - Please help - (reply: 11)
  1447. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
  1448. Cell Count - (reply: 1)
  1449. Generating stable cell line - (reply: 2)
  1450. Harvesting cells off of collagen gels - (reply: 1)
  1451. cells can't be lysed after crosslinking - (reply: 4)
  1452. How many cells to seed? - simple question :) (reply: 5)
  1453. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
  1454. mouse macrophage cell line - (reply: 5)
  1455. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
  1456. cell line information - require (reply: 1)
  1457. Urgent problem - Media change = cells die in 48h (reply: 3)
  1458. recombineering for ES cell targeting - retrieval problems (reply: 1)
  1459. Rat monocyte cell line - (reply: 1)
  1460. How often do you thaw new cells? - passage number or time? (reply: 7)
  1461. Does high speed centrifugation destroy viable cells? - (reply: 2)
  1462. Cell culture - (reply: 7)
  1463. Problems when plating neuroblastoma cells - (reply: 3)
  1464. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
  1465. Cell Doubling Time - (reply: 3)
  1466. Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
  1467. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
  1468. synchronized cells or non-synchronized cells - (reply: 3)
  1469. pre-prepararation of competent cells - (reply: 3)
  1470. ROS accumulation - ROS reactive oxygen species accumulation in cells (reply: 3)
  1471. Bacterial invasion assay with human cells - (reply: 2)
  1472. Measuring gene expression across cell lines - (reply: 2)
  1473. trypan blue without hemocytometer? - (reply: 2)
  1474. How to make an isotonic solution for h9c2 cells - (reply: 2)
  1475. migration < invasion??? - More cells migrating through matrigel than through control inserts (reply: 1)
  1476. Zeocin of G418. Which is the better selection agent for CHO cells? - (reply: 1)
  1477. Immunofluorescence with transfected cells - (reply: 3)
  1478. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  1479. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
  1480. Contamination in cell cultures - (reply: 9)
  1481. DMSO killing more cells at lower concentrations - (reply: 1)
  1482. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  1483. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  1484. lysing cells on ice - (reply: 2)
  1485. Fibroblast cell culture - (reply: 1)
  1486. Cardiac stem cells - (reply: 3)
  1487. How to visualize tumor cells in mice with out sacrificing the animals - (reply: 3)
  1488. cell count and dilution - (reply: 1)
  1489. z competent cells - (reply: 2)
  1490. immunofluorescence staining of sperm cells - (reply: 2)
  1491. Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
  1492. colony formation assay - how many cells to seed (reply: 2)
  1493. Primaria/gelatin plates and gravity-defying fixed cells - (reply: 2)
  1494. Contaminations in cells cultured in suspension - (reply: 2)
  1495. suspension cell transient transfection - (reply: 2)
  1496. What is this ? - Cell culture contaminant (reply: 2)
  1497. cell cryopreservation after siRNA transfection - (reply: 1)
  1498. Cell Digestion Protocol - (reply: 1)
  1499. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
  1500. cell growth - (reply: 4)
  1501. primary cells could not attch to regular polystyrene flasks but immortalised cel - does anybody know what could be happening (reply: 1)
  1502. How to culture cells after sorting? - (reply: 3)
  1503. How to kill nerve cells in a living hydrozoans? - Trying to procude partial/complete nerveless jellyfish (reply: 3)
  1504. lifespan of primary cells in vitro - how long can different cell types survive (reply: 3)
  1505. measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
  1506. T cell differentiation protocols - Th1/Th2/Treg/Th17 (reply: 4)
  1507. Mitochondria extraction from human liver cells - (reply: 1)
  1508. lipofectamine and PEI transfection of hela cells - (reply: 2)
  1509. What can I do whit my Cancer Stem Cells?(I know, its a big question..) - (reply: 7)
  1510. Wash L929 Cells with DPBS (with or w/out CA MG?) - DPBS with or W/out CA,MG? (reply: 2)
  1511. Especially tenacious cells -- what to do? - (reply: 9)
  1512. MDCK cell vascuolation - I got my MDCK cell growing in the T-75 flask (reply: 5)
  1513. Culturing HeLa Cells - (reply: 2)
  1514. expression of His-tagged protein in insect cells - (reply: 2)
  1515. How to isolate vascular smooth muscle cell? - (reply: 1)
  1516. Whole cell lysate including nucelar proteins? - (reply: 3)
  1517. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1518. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
  1519. culturing MCF-7 cellls - clumping and maintaining healthy cells (reply: 3)
  1520. Cell culture media - require information on media (reply: 3)
  1521. how to measure ROS in cell wiht H2DCFH - (reply: 4)
  1522. VEGF in cell nucleus - (reply: 2)
  1523. cell treatment rules - (reply: 4)
  1524. method to seed cells in hyrogels - cell seed in hydrogels (reply: 1)
  1525. transfecting human mesynchemal stem cells - (reply: 5)
  1526. cell schock - (reply: 2)
  1527. Flow cytometry of RFP and CFP expressing yeast cells - (reply: 1)
  1528. Can you store cell pellets for future nuclear extraction? - (reply: 5)
  1529. using cell lysate to run native gel? - (reply: 1)
  1530. Cell Suspension from Frozen Tissue? - (reply: 6)
  1531. looking for stromal cell marker - (reply: 1)
  1532. 'Black dots' in cell culture - (reply: 2)
  1533. ChIP in WT and KO cells - how to analyze - (reply: 1)
  1534. Mouse T cell line - (reply: 1)
  1535. Making competent cell from freeze-thawed stock - (reply: 2)
  1536. Invasion assay using matrigel- cell clumps - (reply: 1)
  1537. producing cell lines - why viruses ?? (reply: 4)
  1538. What color should the cell pellets be after sonication and centrifugation? - (reply: 1)
  1539. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
  1540. Mycoplasma Question - (reply: 2)
  1541. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
  1542. How to detach an antibody from cell surface - (reply: 4)
  1543. Water for electrocompetent cells - (reply: 2)
  1544. Gp 293 Luc cells - (reply: 1)
  1545. how to check cell viability - (reply: 1)
  1546. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  1547. cell fixation - (reply: 1)
  1548. cell detachment in IHC - can I incubate cells with hoechst, heparin and PFA at the same time? (reply: 1)
  1549. cell immobilization for confocal - (reply: 1)
  1550. problem with 293T cell co-transfection - (reply: 5)
  1551. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  1552. Cell Death after formaldehyde treatment - Immunofluorescence (reply: 5)
  1553. Cell Densitometry - (reply: 1)
  1554. My cells died after changing media... - (reply: 6)
  1555. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
  1556. H9C2 cells - trouble when culturing H9C2 cells (reply: 4)
  1557. Contamination of cell ?? - (reply: 2)
  1558. Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
  1559. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  1560. Passaging Cells - (reply: 4)
  1561. Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
  1562. Mysterious cell death - need Hela Help! - (reply: 1)
  1563. NSC-34 Cells sloughing off at media change - (reply: 4)
  1564. Chosing the right competent cells - (reply: 1)
  1565. Cell proliferation assay for LNCaP cells - (reply: 4)
  1566. liquid nitrogen in cell culture - (reply: 2)
  1567. differential cell counting in mixed cultures - (reply: 1)
  1568. Questions about animal cell cultue Please answer - (reply: 1)
  1569. immunofluorescence for HUVEC - how to do immunofluorescence for cancer cells (reply: 3)
  1570. membrane type of cell culture filtration - (reply: 3)
  1571. Critical column of media above cells for access of oxygen - (reply: 1)
  1572. EDTA bacterial cell lysate - (reply: 2)
  1573. Is MTS Assay suitable for Adherent cells? - Confused about MTT Assay and MTS assay.. (reply: 1)
  1574. membrane damaged = cell death? - (reply: 2)
  1575. 293 cells help! - (reply: 1)
  1576. stable cell line - (reply: 2)
  1577. Color of cryopreserved cell line - (reply: 1)
  1578. Established (tumor) cell line - or not...? - (reply: 3)
  1579. Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 1)
  1580. proliferation assay for small cell population - (reply: 2)
  1581. Cell fixation with glutaraldehyde - (reply: 2)
  1582. Yeast contamination in cell culture - hood to blame? - (reply: 6)
  1583. Comparing cell # or DNA content - of pelleted cells (reply: 1)
  1584. bicarbonate in cell culture: powder v. liquid? - (reply: 3)
  1585. cell adhesion assay - collagen (reply: 1)
  1586. To break cell clumps - suspension culture related (reply: 4)
  1587. Problems for MTT in SH-SY5Y cell line - (reply: 1)
  1588. cell stainer size - (reply: 2)
  1589. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
  1590. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
  1591. Low concentration of FBS inhibiting trypsin? - (reply: 1)
  1592. contamination in establishing stable cell lines - (reply: 1)
  1593. How much cell medium for 1 week of culturing? - (reply: 1)
  1594. p53 +/- matched-pair tumor cell lines? - (reply: 2)
  1595. Tranfection cell density and overgrowth - (reply: 3)
  1596. shRNA selection in suspension cell culture - (reply: 6)
  1597. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  1598. Ethanol/methanol/dmso for cell culture - (reply: 1)
  1599. stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
  1600. Rapid Cell Count (in vivo) - (reply: 5)
  1601. cell migration assay (corning transwell) - (reply: 2)
  1602. cell culture in 2 stack flask - (reply: 1)
  1603. Invitogen cell countess - (reply: 5)
  1604. Hypoxia Treatment For Cell Culture - (reply: 4)
  1605. Picking strain for e coli competent cells - (reply: 6)
  1606. cell death - can anybody tell me what process this looks like (reply: 3)
  1607. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
  1608. Marker do discriminate cell cycle phases - G2 phase / Mitosis (reply: 3)
  1609. hematopoietic cell lines - (reply: 3)
  1610. Cos7 Cells to produce retrovirus - (reply: 2)
  1611. how to make nonadhensive cells attach to coverslips without affecting cellular s - (reply: 1)
  1612. Constructing puromycin kill curve - How to count the number of adherent cells without detaching them? (reply: 3)
  1613. stable cell line generation - (reply: 5)
  1614. histological staining for plant cell wall components - (reply: 1)
  1615. My adherent cells are detaching - (reply: 2)
  1616. Cell culture media - DMEM and DMEM/F-12 (reply: 1)
  1617. Cell Cycle analysis of proliferating lymphocytes - (reply: 1)
  1618. Problems with HEK293 cells in 96 well plate format - 293 cells do not adhere well to the plate (reply: 1)
  1619. Cell Synchronization - (reply: 1)
  1620. Slow cell culture growth? - (reply: 3)
  1621. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
  1622. cell lysis buffer - (reply: 1)
  1623. cell aging - cell culture (reply: 2)
  1624. cell concentrations - (reply: 4)
  1625. Floating cells...pls help - (reply: 6)
  1626. Cell migration assay with 3 cell types - (reply: 1)
  1627. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
  1628. How many B cells do I need to get a 1mg of protien - (reply: 2)
  1629. Do histology staningings work in cell culture? - (reply: 2)
  1630. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
  1631. Trypsin Activity Assay - No Change (reply: 4)
  1632. T cell transduction - (reply: 3)
  1633. compound that block DNA replication in human cell - (reply: 1)
  1634. Tranfection of HL 60 cells - (reply: 1)
  1635. mouse liver cell line? - (reply: 1)
  1636. Failed Transformation of Tuner (DE3) cells - (reply: 1)
  1637. Cells suddenly died after changing the medium... why? - (reply: 4)
  1638. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
  1639. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
  1640. CHO Cell contamination - (reply: 6)
  1641. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  1642. seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 1)
  1643. Neuronal precursor cells - (reply: 1)
  1644. Sticky cells - (reply: 2)
  1645. Having problem establishing stable cell line...... - (reply: 5)
  1646. Transferring mamallian cell colonies for selection - (reply: 2)
  1647. Antibody titration and Cell number - (reply: 1)
  1648. Cell Invasion Assay - possible to analyze with flow cytometry? (reply: 5)
  1649. Cell culture contamination - "outsider I.D." (reply: 2)
  1650. Alternative media for PC-12 cells - (reply: 2)
  1651. viability of adherent cells - (reply: 3)
  1652. How long can viably frozen cells stay good in -80 degree? - (reply: 5)
  1653. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
  1654. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
  1655. HeLa cells and BCL 2 expression - (reply: 1)
  1656. B104 cells - (reply: 2)
  1657. SH-SYS5 cell culture for dummies... - (reply: 4)
  1658. collagen staining in cell culture - (reply: 5)
  1659. Hematopoietic lineage depletion kit - Hematopoietic stem cell enrichment kits (reply: 4)
  1660. changing cell shape - (reply: 2)
  1661. how to change cell shape? - (reply: 3)
  1662. About T-antigen and tumor cell lines - (reply: 2)
  1663. Sub-culture of N2a cells - (reply: 1)
  1664. Haematopoietic stem cells - (reply: 5)
  1665. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
  1666. proteomics - minimum cell number (reply: 1)
  1667. Standard Curve for OD Turbidity vs Cell density - (reply: 6)
  1668. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
  1669. Plasmids for protein expression in mammalian cells - (reply: 2)
  1670. cell lysis after induction - (reply: 1)
  1671. competent cell preparation? - (reply: 1)
  1672. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  1673. Dendritic cell infection - (reply: 2)
  1674. Competent Cells - (reply: 5)
  1675. looking for bovine skeletal muscle cells - (reply: 5)
  1676. my cell line doesnt change the colour of the media - even when cells are 100 % confluent (reply: 4)
  1677. Dead Dendritic cells ... please help - (reply: 1)
  1678. Cell concentration Question - (reply: 3)
  1679. cho cell line adoptation to serum free medium - (reply: 1)
  1680. Problems with viability of Jurkat's cells - (reply: 3)
  1681. THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells (reply: 1)
  1682. 24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
  1683. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  1684. Culturing U2OS cells - (reply: 1)
  1685. staph cell count - (reply: 5)
  1686. What kind of speed for HepG2 cells. - (reply: 2)
  1687. Serum free culture media 293T cells? - (reply: 1)
  1688. Embryoid bodies - How to differentiate mouse ES cells quickly and efficiently (reply: 8)
  1689. cell count - is it right? (reply: 3)
  1690. Haemocytometer cell count - Coverslips (reply: 4)
  1691. Growing two cell types in the same flask?? - (reply: 1)
  1692. Cell isolation - (reply: 1)
  1693. trouble to grow cells on top of coverslip - (reply: 6)
  1694. Immortalized murine hepatocytes - (reply: 1)
  1695. about measuring luciferase activity in living cells - (reply: 8)
  1696. Culture conditions for cell treatments - (reply: 4)
  1697. preadipocyte 3T3-L1 cells question? - (reply: 1)
  1698. SH-SY5Y cell culture - Suspended cells (reply: 4)
  1699. Magnetic-activated cell sorting ? - (reply: 4)
  1700. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
  1701. How to increase IF signal in acetone/methanol fixed cells? - (reply: 5)
  1702. Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
  1703. sheep red blood cell rosetting protocol - (reply: 1)
  1704. Tranformation cell harvesting - (reply: 5)
  1705. self-renewal Vs proliferation - stem cells (reply: 3)
  1706. Plyse cell and DH5a - (reply: 1)
  1707. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  1708. INS-1 cell line - (reply: 1)
  1709. Central cell seeding - (reply: 4)
  1710. please help with Freezing primary cells - (reply: 1)
  1711. COMPETENT CELL PREPARATION - (reply: 6)
  1712. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  1713. suggest cells expressing low levels of adhesion molecules - (reply: 1)
  1714. Cell Culture and Pipette Tips - (reply: 4)
  1715. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  1716. Cell counting issues - the ring of death on my flasks (reply: 5)
  1717. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  1718. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
  1719. RNA Isolation - RNA isolation from human cell lines (reply: 2)
  1720. Cell Fixation - methanol fixation of cells (reply: 3)
  1721. How to retrieve my human blood immune cells from liquid nitrogen without killing - (reply: 3)
  1722. stem cells and EB (Embryoid bodies) - (reply: 1)
  1723. Immunohistchemistry of red blood cells - (reply: 1)
  1724. cell cryosection - (reply: 5)
  1725. Shifting of entire cell cycle to the left - (reply: 3)
  1726. freezing suspended cells - (reply: 7)
  1727. Cell Brakeage...how will I know - I need the mitochondria. (reply: 1)
  1728. How many cells... - ...gives you how much RNA? (reply: 2)
  1729. Cell culture - Culture medium (reply: 1)
  1730. Anybody ever use whole blood plasma serum stimulate cells? - (reply: 2)
  1731. How can I separate my cell line from feeder cells - (reply: 3)
  1732. ChIP-seq: Minimum number of cells - (reply: 3)
  1733. CHO or VERO? - Cell culture (reply: 2)
  1734. Media for squamous cells - (reply: 3)
  1735. how to trace single cell movement throughout gastrulation of zebrafish - (reply: 4)
  1736. Oxygen diffusion through cell culture medium - (reply: 2)
  1737. Request for Mono Mac-1 cell line - (reply: 1)
  1738. help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
  1739. How much sonication power needed? - protein extraction from cells (reply: 2)
  1740. protein extraction - from cell line in T25 flask (reply: 5)
  1741. Getting cells from flasks - (reply: 5)
  1742. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  1743. Fixation of immune cells - "how to" protocol needed (reply: 2)
  1744. cell cycle analysis by FACS - (reply: 2)
  1745. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  1746. Trypsin Expression and Purification - (reply: 3)
  1747. cell cycle analysis PI staining shift - (reply: 1)
  1748. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
  1749. Problem with stable overexpression cell lines - (reply: 3)
  1750. seeking help in stable cell line generation - (reply: 3)
  1751. SDS, Cell numbers, dilution - (reply: 18)
  1752. Help with Hep G2 cells - (reply: 1)
  1753. Floating cells in adherent VERO culture - contamination? (reply: 2)
  1754. RAW264.7 cells+nitric oxide determination - (reply: 13)
  1755. BL 21 cells - (reply: 4)
  1756. Mutated MDCK cells - protocol for mutating MDCK cells (reply: 1)
  1757. Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
  1758. Cell pellet pesuspension without Vortexing or pippeting - seeking advice - (reply: 2)
  1759. western blot problem: cell culture VS mouse tissue - (reply: 4)
  1760. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 2)
  1761. Detachment of very adherent cells for flow cytometry - (reply: 7)
  1762. THP-1-Blue-CD14 cells - (reply: 4)
  1763. are cells prone to swelling in PBS after sorting - (reply: 3)
  1764. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
  1765. suspension cell culture - optimum cell number in plates (reply: 2)
  1766. Whole tissue starting product - not cultured cells - (reply: 2)
  1767. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
  1768. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  1769. Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
  1770. culturing COS-7 cells - (reply: 5)
  1771. Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
  1772. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  1773. Loss of cell adherence after transfection - The first passage after selection process cells lost adherence (reply: 2)
  1774. how will i be able to culture tumor cells? - (reply: 3)
  1775. General cell lysis question - whole cell lysates need to be centrifuged? (reply: 2)
  1776. Rnase for cell cycle - (reply: 5)
  1777. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
  1778. cell loss upon centrifugatino - (reply: 10)
  1779. cell lines vs tissue expression - (reply: 1)
  1780. Is molecular biology grade DMSO good enough for cell stock? - (reply: 2)
  1781. Clonogenic assay with agressive cell line - (reply: 4)
  1782. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  1783. Number of cells in 6 wells-plate well - (reply: 1)
  1784. protein extraction from primary cells - (reply: 1)
  1785. Extracting proteins from cells - (reply: 1)
  1786. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
  1787. strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 6)
  1788. cell floating in 96-well plate - (reply: 1)
  1789. Inducing Adherence in Suspension Cells - (reply: 1)
  1790. Isolated nuclei and trypan blue - Silly question .......... (reply: 2)
  1791. bacterial contamination in cell culture - (reply: 3)
  1792. THP-1 cell culture - (reply: 1)
  1793. H9c2 cell contamination? - (reply: 4)
  1794. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  1795. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
  1796. What stains red in trypan blue - (reply: 2)
  1797. transfection in floating cells - (reply: 5)
  1798. immortal or stable cells - primary human cells to immortal cell line? (reply: 3)
  1799. SDS-PAGE Cell lysis problems - (reply: 3)
  1800. Storage of invitrogen top10 cells - (reply: 11)
  1801. oxidative stress - how to measure oxidative stress in cells (reply: 2)
  1802. Cells clots forming during preparation mouse splenocytes - (reply: 4)
  1803. Transwell Cell Migration/Invasion Assay - (reply: 4)
  1804. neuronal nitric oxide synthase/cell line - (reply: 3)
  1805. HL 60 cells passage - (reply: 1)
  1806. Establishing a new cell line - (reply: 1)
  1807. Isolating Natural Killer cells from whole blood - (reply: 2)
  1808. Centrifugation of epithelial cells - (reply: 2)
  1809. cell engineering exam - (reply: 5)
  1810. Cell detachment: alternative to trypsine/EDTA? - (reply: 3)
  1811. Cell supplier - (reply: 2)
  1812. Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
  1813. Mir34a positive control cells - (reply: 4)
  1814. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
  1815. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
  1816. FACS positive cells in negative controls help - (reply: 1)
  1817. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
  1818. How to separate fungus cells from human lung tissues - (reply: 7)
  1819. Growing of TOP10 chemically competent cells - (reply: 4)
  1820. Stable Cell Selection with Neomycin - (reply: 4)
  1821. endogenous miRNA of cos-7 cell - (reply: 2)
  1822. Qustion about MDA-MB-157 cells culture - (reply: 4)
  1823. Folic acid solution for cell culture - (reply: 2)
  1824. Mycoplasma contamination - (reply: 6)
  1825. Strange dots around the cell+Photo - (reply: 6)
  1826. FACS of GFP expressing cells from mice brains - (reply: 1)
  1827. cells can't attach in the flasks - (reply: 4)
  1828. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  1829. Cell fractionation and isolation of nuclei - (reply: 1)
  1830. Methanol toxicity in cell culture - (reply: 15)
  1831. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
  1832. Freezing cells, quick question - (reply: 1)
  1833. Best place to buy cells - (reply: 2)
  1834. help on generation of stable cell lines with pBabe vector - pBabe-puro, phoenix Ampho cells (reply: 7)
  1835. DMSO usage in cell culture - (reply: 9)
  1836. stopping brief cell treatments - (reply: 2)
  1837. Phenol red in media - What metabolic products of cells are acidic? (reply: 2)
  1838. Cell culture container - (reply: 2)
  1839. cell lysis method - (reply: 1)
  1840. Dendritic cell immunology - activated naive cells? (reply: 9)
  1841. cell contamination???? - (reply: 1)
  1842. counting number of cells - (reply: 6)
  1843. Stacking of RAW 264.7 cells - (reply: 1)
  1844. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
  1845. Lentiviral transduction of B cells - (reply: 7)
  1846. Lentivirus transduction of spleen cells - (reply: 2)
  1847. trouble with establishing a stable cell line - (reply: 3)
  1848. Cell culture conditions and antigen expression - (reply: 1)
  1849. Phoenix packaging cell and diphtheria toxin - (reply: 3)
  1850. Gene expression in cell line - (reply: 1)
  1851. isoflurane and FACS analysis of peripheral blood? - my cells look terrible everytime I use isoflurane to anaesthetize (reply: 5)
  1852. Growing Up Cells - (reply: 3)
  1853. protein extraction after cell lysing - (reply: 2)
  1854. cell line for neurodevelopment - (reply: 2)
  1855. Difficulty in doing hemocytometer cell count - (reply: 4)
  1856. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
  1857. Method for lysing cells for fractionation - (reply: 4)
  1858. Neuronal cell culture - why use neonatal brains? - (reply: 1)
  1859. Detect gene in stable cell line, - (reply: 4)
  1860. staining cells - (reply: 3)
  1861. pIRESneo for mammalian cells transfection - (reply: 4)
  1862. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  1863. H9C2 - cell line - Need clarification (reply: 1)
  1864. Cell plating calculation !! - Help needed with Cell plating calculation !! (reply: 2)
  1865. Cell lines to study epigenetics - (reply: 2)
  1866. How to transfect two plasmids in a cell - (reply: 10)
  1867. Cells dying before i can get them to in vitro assay - (reply: 1)
  1868. Strange bands in western of RPE-1 cells - (reply: 1)
  1869. Semisolid Medium For HL 60 cells - (reply: 4)
  1870. Cell culture contamination (do not know what it was) - (reply: 1)
  1871. questions about cell lysis - (reply: 7)
  1872. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  1873. How to homogenize cell without disruption of nuclei? - (reply: 2)
  1874. Harvesting cells for flow cytometry - (reply: 10)
  1875. culture C6/36 insect cell lines - (reply: 1)
  1876. seeding cell densities - seeding cells at same density (reply: 1)
  1877. cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
  1878. Culturing QT6 cell line - Ideal culture conditions (reply: 2)
  1879. Calculating MOI for luciferase-transducted cells. - Would the sensitivity of a fluorescent microscope be enough to see the (reply: 3)
  1880. Picking epithelial, endothelial and neural cell lines - Please suggest best models (reply: 2)
  1881. How to fix suspencion cells for DAPI detecting under the microscope? - (reply: 6)
  1882. mycoplasma growth - (reply: 3)
  1883. primary lung epithelial cells - (reply: 1)
  1884. Generating double stable cell line - (reply: 3)
  1885. HACAT cells - very low cell viability after thawing cells (reply: 18)
  1886. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
  1887. My cells suddenly die !!! - Please help me ! why is this ? (reply: 11)
  1888. database for mutations in cancer cell lines? - (reply: 2)
  1889. Sonication Problems, Branson 450, LNCaP cells - (reply: 4)
  1890. ? Definition of Passage for suspension cells - (reply: 1)
  1891. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1892. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  1893. Chip problem: antibody or cell number?? - (reply: 6)
  1894. stable cell line - (reply: 1)
  1895. microbial cell viability - any recommendations - assays (reply: 1)
  1896. SF9 cells not adhering - (reply: 10)
  1897. HeLa cells - Growth of thawed HeLa cells (reply: 7)
  1898. Dendritic Cells and Flow Cytometry - (reply: 11)
  1899. direct cell lysate - (reply: 8)
  1900. How long can I wait after seeing HeLa cells till transfection - will it affect my Efficiency (reply: 6)
  1901. Culturing/Mounting of polarised cells for confocal microscopy - (reply: 1)
  1902. Intestinal cell culture: Protocol required - (reply: 1)
  1903. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
  1904. Carbon di oxide free cell culture - (reply: 1)
  1905. surface staining of THP-1 cell - (reply: 2)
  1906. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  1907. Cell Wall Lysis For Dummies - (reply: 4)
  1908. Tissue & Cell Fixation solutions - (reply: 3)
  1909. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1910. Treating cells with chemicals and how to dilute into medium - (reply: 5)
  1911. T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (reply: 6)
  1912. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1913. Has anyone used COS-7 cells for retina? - (reply: 1)
  1914. How to perform a growth curve? - i need to compare 3 cell lines for growth (reply: 2)
  1915. siRNA concentration in divinding-growing cells - (reply: 2)
  1916. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  1917. cell culture guideline from abcam - (reply: 5)
  1918. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  1919. problems with detecting protein expression - protein expression in cell (reply: 4)
  1920. freeze PBMC and cell sorting? - (reply: 1)
  1921. Control vehicle treatment in cells - (reply: 2)
  1922. Cancer cell culture exploded onto my face - (reply: 8)
  1923. FACS analysis of GFP expressing cells - Fix or not - (reply: 10)
  1924. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
  1925. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
  1926. Heterogeneous methylation in a same cell line - (reply: 1)
  1927. siRNA cell function examples - (reply: 1)
  1928. Good comprehensive resource for maintaining immortal cell lines? - (reply: 1)
  1929. DH5a comp cells - (reply: 5)
  1930. Trypsinized cells after fixation - (reply: 3)
  1931. Detaching Hela cells - (reply: 3)
  1932. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
  1933. cell cycle softawe - (reply: 3)
  1934. How to maintain cells at a specific apoptosis stage - (reply: 12)
  1935. Which cell line produce cytokine? - (reply: 13)
  1936. Cell Viability - (reply: 1)
  1937. Shipping of cryopreseved cell - (reply: 15)
  1938. To what extent competent cells be thawed before transformation? - (reply: 4)
  1939. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
  1940. Storing HEK293 cells at -80C - (reply: 5)
  1941. How to control cloned cDNA expression level in cells? - (reply: 8)
  1942. Asses mycoplasma contamination - (reply: 6)
  1943. RNA isolation following apoptotic cell labeling - (reply: 3)
  1944. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
  1945. Is there an optimal incubation time for cell lysis? - (reply: 7)
  1946. The way to count adherent cells in 96 well - (reply: 3)
  1947. lysing cells in LSB - (reply: 2)
  1948. Using NIH3T3 cells of look at MAPK activation by Ras - (reply: 1)
  1949. Staining for neural crest derived cells?? - (reply: 2)
  1950. Dendritic_ cells - (reply: 3)
  1951. problem to stain just one cell line in coculture - (reply: 4)
  1952. Inactivation of trypsin - (reply: 4)
  1953. Culturing stromal cells - (reply: 2)
  1954. A549 cells - (reply: 4)
  1955. Cunterstaining Trypan blue - (reply: 6)
  1956. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
  1957. Protein extraction from cell culture media - (reply: 9)
  1958. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
  1959. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
  1960. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  1961. can we prepare Copmetent cell by calicum cloride with 0.09 OD - Cp cells with 0.09 OD (reply: 3)
  1962. electroporation of HL60 cells - (reply: 1)
  1963. Intestinal epithelial cell culture - (reply: 4)
  1964. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
  1965. Easiest cell lysis protocol - no interest in preserving proteines (reply: 4)
  1966. passage number for cell line - (reply: 7)
  1967. MOCK INFECTED CELLS - (reply: 4)
  1968. cuboidal cells - (reply: 1)
  1969. Cell count using a hemocytometer - (reply: 4)
  1970. Hepatocyte culture - (reply: 4)
  1971. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
  1972. what is mock cell line? - (reply: 4)
  1973. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
  1974. How to seed the cells evenly in 6 wells plate - (reply: 13)
  1975. knockout mouse with increase cell size - (reply: 3)
  1976. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
  1977. Paper on energy requirement of cells - (reply: 2)
  1978. galactose induction of yeast cells - (reply: 2)
  1979. making competent E coli DH5a cells - (reply: 6)
  1980. How could you explain these results? - cancer cells are weird (reply: 1)
  1981. strange ES cells contamination - please help!!! (reply: 1)
  1982. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)
  1983. How to culture PC3 cell line - (reply: 3)