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Cell culture Related Discussions

1. Cells adhering to walls of conical tube? - (reply: 4)
   
2. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 1)
   
3. choosing the correct subculturing ratio - ovarian cancer cell lines - (reply: 1)
   
4. RAW 264.7 cell line - (reply: 1)
   
5. washing of attached bacterial cells - (reply: 3)
   
6. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
   
7. Cell Debris Isolation From Blood - (reply: 3)
   
8. How to calculate drug amount to use in cell lines - (reply: 2)
   
9. Growing certain number of bacterial cells in LB - (reply: 1)
   
10. T cells or Lymphocytes? - (reply: 2)
    
11. Western Blot with fixed cells - (reply: 5)
    
12. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
    
13. How to culture HK-2 cells - (reply: 6)
    
14. competent cells - (reply: 1)
    
15. Debris in cell culture - (reply: 1)
    
16. What housekeeper to use with HEK cells? - (reply: 3)
    
17. Housekeeper for HEK cells? - (reply: 1)
    
18. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
    
19. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
    
20. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
    
21. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
    
22. Gene expression profiling with small cell number - (reply: 1)
    
23. Gene expression assay with small cell number - (reply: 2)
    
24. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
    
25. Stem Cell Therapy comes of age? Discussion Topic - (reply: 1)
    
26. cell free DNA and maintaining stability - (reply: 3)
    
27. Freezing Cells For Use In Assays - (reply: 2)
    
28. INS-1E cells migration assay - (reply: 1)
    
29. Short-term storage of HEK293 cells before FACS - (reply: 3)
    
30. Sample storage for ELISA (cell culture supernatant) - (reply: 2)
    
31. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
    
32. Primary cell line - (reply: 4)
    
33. 2D Image Primary Cell line - (reply: 13)
    
34. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
    
35. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
    
36. To small amount of cells used for shRNA transfection - (reply: 1)
    
37. using cell line to generate standard curve - (reply: 6)
    
38. Autofluorescence in thick structure cluster of cells - (reply: 4)
    
39. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
    
40. Serial dilution of cells - (reply: 3)
    
41. Cloning repetitive sequences in mammalian cells - (reply: 3)
    
42. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
    
43. Plant Cells - what are they? - (reply: 1)
    
44. HS-5 and HS27A cells - (reply: 1)
    
45. MC-3T3 cells are not mineralizing... - (reply: 2)
    
46. HS-5 cell culture "question" - (reply: 1)
    
47. G418(Geneticin) is not killing my cells - (reply: 2)
    
48. Community feedback needed to clarify terms for a Cell Culture Ontology - (reply: 1)
    
49. Quantify total DNA in small cell lysate...? - (reply: 1)
    
50. Trouble validating in multiple cell types - (reply: 1)
    
51. Gating of dendritic cells - (reply: 7)
    
52. Please help me take a look at my cells to see whether is it contaminated - (reply: 3)
    
53. cell binding assay with nanoparticles? - (reply: 2)
    
54. Cell lines for clonal selection - (reply: 3)
    
55. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
    
56. SH-SY5Y Immortalized cell lines; researching neurotoxicity? - (reply: 3)
    
57. Pelleting bacterial cells - (reply: 1)
    
58. How viable is cell culture for a model? - (reply: 2)
    
59. Help with adherent cell culture - (reply: 7)
    
60. study of receptors in cell lines - (reply: 1)
    
61. Cell line nomenclature - suffixes ? - (reply: 3)
    
62. Maintainence of transformed cells - (reply: 5)
    
63. improving viability of pelleted cells - (reply: 2)
    
64. Buying Ins-1e cells - (reply: 1)
    
65. How do/should cell biologists calculate power of analysis? - (reply: 1)
    
66. Mycoplasma on normal hands? - (reply: 1)
    
67. comparing ADCC effect in 3 cell lines. - (reply: 1)
    
68. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
    
69. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
    
70. Trypan blue - (reply: 5)
    
71. virus and cell surface receptor interaction - (reply: 4)
    
72. Music Video on Cell Division/Mitosis - (reply: 3)
    
73. How to select competent E. coli cells? - (reply: 7)
    
74. Host cell protein sequence search - (reply: 1)
    
75. qPCR from HEK cells transfected with Plasmid - (reply: 4)
    
76. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
    
77. Preparing supernatant from cell culture for western blot - (reply: 4)
    
78. Fast/easy way to measure cell size - (reply: 4)
    
79. Which is the best way to generate growth curves for cancer cell lines - (reply: 6)
    
80. Cell number issue - Nile Red staining - (reply: 3)
    
81. Music Video on Cell Division/Mitosis - (reply: 1)
    
82. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
    
83. Integrin B1 Staining in HT1080 cells - (reply: 1)
    
84. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
    
85. My cells won't pellet - any ideas? - (reply: 6)
    
86. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
    
87. EMSA: cell extract vs purified protein - (reply: 2)
    
88. Insect cells transfection reagent - (reply: 1)
    
89. Low Protein Content In Cell Lysate - (reply: 1)
    
90. Can you see GFP after lysis mammalian cells? - (reply: 5)
    
91. Centrifugation speeds for cells. - (reply: 8)
    
92. Cell recovery from ethanol fixation - (reply: 4)
    
93. Cryopreservation of MSC at lower cell density - (reply: 2)
    
94. C-terminal GST fusion problem in mammalian cell - (reply: 6)
    
95. Identify cell culture contamination - (reply: 2)
    
96. Adipose stem cell culture - (reply: 5)
    
97. Determine cell proliferation by viability assay ? - (reply: 6)
    
98. Apoptosis via cell to cell contact - (reply: 1)
    
99. Cells stop to grow - (reply: 6)
    
100. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
     
101. Long Treatment, Rapidly Dividing Cells - (reply: 7)
     
102. Need help calculating cell number to plate - (reply: 1)
     
103. Injection of human cell culture conditioned media into nude mice - (reply: 1)
     
104. How long can I store cell lysis buffer at 4C for? - (reply: 1)
     
105. Heeeeeelp..cell cycle sample..is it possible to store it? - (reply: 2)
     
106. Subculture issue of primary cell - (reply: 6)
     
107. Differentiating mouse from human cells with PCR - (reply: 5)
     
108. primary valve interstitial cells transfection - (reply: 1)
     
109. What to do about mycoplasma? - (reply: 9)
     
110. Stem Cell Donation - (reply: 5)
     
111. isolating DNA from cells in suspension - (reply: 5)
     
112. Software for designing cell pathways - (reply: 1)
     
113. Centrifuged cells stick to wall of reaction tube - (reply: 12)
     
114. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
     
115. Mycoplasma Test Kit - (reply: 1)
     
116. Changing Genomic Regions in Host Cell - (reply: 1)
     
117. TRYPSIN - (reply: 4)
     
118. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
     
119. my competent cell grow on any agar plate - (reply: 7)
     
120. Bacterial Contamination in HuEmbryonic cell lines seen in SFM4 but not in DMEM + - (reply: 3)
     
121. Cell attachement occuring after 48 hours, not 24 hours. - (reply: 3)
     
122. Transfection of a non human gene into human cell line - (reply: 1)
     
123. Procedures to replace vial of cells and passage number. - (reply: 4)
     
124. Immunofluorescence on cells - can I keep the coverslips unstained in PFA? - (reply: 4)
     
125. p14ARF and MDM2 in transformed cells - (reply: 1)
     
126. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
     
127. contamination of human cell lines with yeast - (reply: 3)
     
128. Generation of single cell suspensions from neural tissue - (reply: 2)
     
129. Is this contamination or cell death? - (reply: 7)
     
130. BaF3 cell culture in 96 well plates - (reply: 2)
     
131. THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS) - (reply: 2)
     
132. unwanted cell lysis during LB+Amp incubation - (reply: 5)
     
133. Cells spontaneously detaching? - (reply: 1)
     
134. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
     
135. different type of media for cell culture - (reply: 1)
     
136. Influenza: cell and mechanism information? - (reply: 1)
     
137. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
     
138. How to treat cells with TNFa - (reply: 2)
     
139. CLOUDINESS of RIN5F cell culture - (reply: 7)
     
140. U87MG cells not growing well - (reply: 3)
     
141. Concentrating low volume of cells - (reply: 8)
     
142. Preparing a conditioned medium from cancer cells - (reply: 3)
     
143. Presence of melanin in melanin containing cell line - (reply: 1)
     
144. Cancer cells culture - (reply: 1)
     
145. How to achieve uniform cell distribution in culture vessle - (reply: 5)
     
146. viability/integrity stain of fungal cells - (reply: 5)
     
147. Cell culture contamination - (reply: 3)
     
148. airway primary cells stop growing at passage 3 - (reply: 1)
     
149. cell culture neural cells easier? - (reply: 6)
     
150. Question - Corticosterone, cell culture - (reply: 1)
     
151. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
     
152. Where to obtain rat embryonic stem cells? - (reply: 3)
     
153. Stable cell line - (reply: 1)
     
154. PBMC cells handling - (reply: 1)
     
155. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
     
156. Unable to clone in E. coli cells - (reply: 3)
     
157. removing MHC class I from the cell surface - (reply: 3)
     
158. Osteosarcoma cell culture problem - (reply: 7)
     
159. rtPCR stem cell signalling array from quiagen- has anybody experience? - (reply: 1)
     
160. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 4)
     
161. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 1)
     
162. colon cancer cell lines vs normal colon epithelial cells - (reply: 1)
     
163. Cell count and seed, plz help... - (reply: 4)
     
164. In and out of cell Help 2 - (reply: 6)
     
165. In and out of cell Help - (reply: 2)
     
166. Primary culture of breast tumor cells - (reply: 1)
     
167. Strange Cell Morphology & Cell Debris Following Lentiviral Transduction - (reply: 2)
     
168. Cell numbers versus confluency - (reply: 2)
     
169. Flow cytometry in THP-1 cells - (reply: 1)
     
170. Competent bacterial cells - (reply: 2)
     
171. Could a change of FBS affect outcome of a cell proliferation assay? - (reply: 4)
     
172. How do they make passage limited cells? - (reply: 1)
     
173. transient transfection problem using ME180 cells - (reply: 1)
     
174. How to completely remove cells from surface - (reply: 3)
     
175. Hiperfect siRNA in HT-29 cells help - (reply: 2)
     
176. Isolation of BOTH cells AND serums from mouse blood - (reply: 5)
     
177. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
     
178. Commercial source Stomach Smooth Muscle Cell lysate - (reply: 1)
     
179. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 1)
     
180. cell line contaminated by something... - (reply: 2)
     
181. Flag Staining in U87 cells - (reply: 4)
     
182. protein is not expessing in HEK 293 cells - (reply: 2)
     
183. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 11)
     
184. HepG3 cell line origin - (reply: 3)
     
185. dilution of cell suspension - (reply: 4)
     
186. how to preserve spleen cells for long duration, for flowcytometry - (reply: 2)
     
187. Increasing cell growth rate in plain M9 medium - (reply: 1)
     
188. Puromycin - shRNA - stable cell line for knock down - (reply: 4)
     
189. Reducing cell adhesion - (reply: 1)
     
190. The Hek 293T cell line looks like non adherent cells after to be sorted in FACS - (reply: 1)
     
191. Hygromycin selection of virally-transduced cell lines - (reply: 1)
     
192. Which cells express which genes? - (reply: 1)
     
193. IGF1 stimulation of THP1 cells - (reply: 2)
     
194. Cells detach during bodipy 493/503 staining - (reply: 1)
     
195. RNA isolation from small numbers of cells - (reply: 6)
     
196. Distinguish murine and human cells by surface staining? - (reply: 1)
     
197. starting HaCaT cell line. basic questions - (reply: 6)
     
198. culture media for pancreatic cancer stem cells - (reply: 2)
     
199. Lysis method for single human embryonic cell - (reply: 5)
     
200. In vitro transcription/translated protein to cells!!! - (reply: 1)
     
201. Cell plating - (reply: 2)
     
202. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
     
203. colony formation assay in A2780 cells - (reply: 4)
     
204. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
     
205. Which is a better way to culture cells after thawing ? - (reply: 2)
     
206. Comet assay sperm cells - (reply: 5)
     
207. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
     
208. Live/Dead Staining of already Permeable Cells - (reply: 10)
     
209. cell synchronisation for fibroblasts (help choosing method/chemical) - (reply: 2)
     
210. Cell Therapies - (reply: 2)
     
211. What is the activity of 1X Trypsin solutions? - (reply: 3)
     
212. SURE E. coli competent cells - (reply: 1)
     
213. Stable protein expression in Vero cells - (reply: 2)
     
214. HL60 cell line suppliers - (reply: 1)
     
215. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
     
216. Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
     
217. Question about HeLa cell overconfluence - (reply: 3)
     
218. 293T cell contamination post-transfection - (reply: 1)
     
219. HEK293T cells are too fragile?? - (reply: 2)
     
220. number of splitting after defrozing cells - (reply: 2)
     
221. my human iPS cells have a lot of differentiation - (reply: 4)
     
222. Properly designing a drug treatment assay measuring cell cycle arrest - (reply: 2)
     
223. Detaching Breast Cancer cells (MCF-10A, HCC70) without trypsin- used for FACs - (reply: 4)
     
224. counting cells before adding them to 96 well plate - (reply: 7)
     
225. Cell Suspension - (reply: 2)
     
226. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 2)
     
227. MDA-MB-231 cells undergoing senescence- help! - (reply: 2)
     
228. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
     
229. coating cover-slides before seeding cells for fluorescent microscopy - (reply: 4)
     
230. How to induce early apoptosis in cells without using chemicals or UV - (reply: 5)
     
231. choice of cell line for orthotopic xenograft implant - (reply: 3)
     
232. Compound concentration depending on cell number? - (reply: 4)
     
233. How to remove mycoplasma contamination in cell lines? - (reply: 5)
     
234. [Question] How to remove cell debris stuck on the attached cells in primary card - (reply: 2)
     
235. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
     
236. Reagent used to adjust pH of cell culture media - (reply: 3)
     
237. Powder indomethacin in cell culture media?? - (reply: 2)
     
238. How many generation of HEK293 cell can undergo? - (reply: 1)
     
239. [Question] how to make primary-cultured cells evenly distributed? - (reply: 11)
     
240. small dots in cell culture - (reply: 2)
     
241. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
     
242. Clumpy competent cells - (reply: 3)
     
243. Preparation of bacterial cells for SEM and TEM - (reply: 2)
     
244. G418 advice in estab;lishing stable cell line - (reply: 1)
     
245. Electroporation of J774.2 murine macrophage cell line - (reply: 1)
     
246. About trypsinizing adherent cells in 96-well plates - (reply: 2)
     
247. Passaging cells - (reply: 12)
     
248. Preparing homemade XL1-blue cells in CCMB80 buffer - (reply: 1)
     
249. Understanding Cell Passaging - (reply: 3)
     
250. How to extract DNA from small number of cells - (reply: 2)
     
251. How to identify beta pancreatic cells in dish of heterogenous cells? - (reply: 12)
     
252. Cell Splitting Ratio Question - (reply: 5)
     
253. Sf9 cell suspension culture - (reply: 5)
     
254. competent cell formation - (reply: 1)
     
255. Help! Clontech Xfect is killing my T-Rex HeLa cells!!! Why! - (reply: 1)
     
256. How long are cells viable on ice - (reply: 2)
     
257. Discrepancy between tissue and cell culture - (reply: 1)
     
258. What is the density of a human skin cell? - (reply: 8)
     
259. Determining the % of GFP/CHERRY cells in co-mixed culture - (reply: 3)
     
260. Is the genomic DNA similar among different cell lines? - (reply: 4)
     
261. serumm free culture of adherent cho cells - (reply: 1)
     
262. detection of mesenchymal stem cells in cancer tissue sample - (reply: 1)
     
263. Can u suggest any myoepithelial cell isolation protocol with the help of reagent - (reply: 1)
     
264. cell line cultured in suspension - (reply: 1)
     
265. GFP cells in paraffin.....orange? - (reply: 1)
     
266. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
     
267. Cell staining for fluorometric assay - (reply: 5)
     
268. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
     
269. Long-Term Culturing of SH-SY5Y Cells - (reply: 1)
     
270. N acetyl cysteine solution for cell culture - (reply: 3)
     
271. Aortic smooth muscle cell contamination - (reply: 1)
     
272. need recommendation for stable transduction of fibroblast cells - (reply: 1)
     
273. Sub G1 in cell cycle - (reply: 2)
     
274. DMSO induces cluster growing in LNCaP cells? - (reply: 1)
     
275. Cell cycle arrest - adherent cells going into suspension - (reply: 4)
     
276. Prevent adherent cells from adhering - (reply: 3)
     
277. INS-1E cells not adhere to the flask! - (reply: 1)
     
278. A549 cells are not attaching to flask - (reply: 2)
     
279. irradiated J774 cells - (reply: 1)
     
280. Cell lysis with proteinase k - (reply: 2)
     
281. One siRNA sequence, many cell lines - (reply: 1)
     
282. Freezing cell lysates for westerns - (reply: 2)
     
283. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
     
284. adherence to host cell question - (reply: 2)
     
285. New patch of sf9 cells not growing - (reply: 2)
     
286. contamination in yeast cell culture - (reply: 8)
     
287. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
     
288. sf9 Insect cells questions - (reply: 3)
     
289. Kit to mesure cell death - (reply: 4)
     
290. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
     
291. J774 and Flow cytometry....are they really big cells ? - (reply: 9)
     
292. immunostaining cells grown in matrigel - (reply: 1)
     
293. Cell concentration for flow cytometry - (reply: 2)
     
294. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
     
295. Cells growing very slowly - (reply: 5)
     
296. SH-SY5Y cells do not grow - (reply: 1)
     
297. GH3 cells - (reply: 4)
     
298. The connection between LPS and PMA stimulation for maturation of ThP1 cells - (reply: 2)
     
299. Using normal competent cells in electroporation method? - (reply: 1)
     
300. Washing n2a cells out of 75 cm flask? - (reply: 1)
     
301. How to dissociate N2A cells after centrifugation? - (reply: 3)
     
302. Undefinable particles in cell culture flask - (reply: 2)
     
303. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
     
304. immunostaining of cells in culture - (reply: 1)
     
305. healthy stem cells vs cancer stem cells - (reply: 1)
     
306. values for mRNA molecules per cell - (reply: 1)
     
307. Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
     
308. MCF10A Tet-ON stable cell line generation - (reply: 1)
     
309. Primary cell line - (reply: 2)
     
310. Neurosphere Assay with neuroblastoma cell lines - (reply: 1)
     
311. How to decontaminate adherent cells with gentamicin - (reply: 2)
     
312. Contamination in CHO cell ? - (reply: 3)
     
313. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
     
314. How to purify RNA from a handful number of cells? - (reply: 5)
     
315. Check cell viablity after FACS - (reply: 2)
     
316. ISH on fluorescing cells - (reply: 1)
     
317. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
     
318. Protein extraction from dead cells - (reply: 1)
     
319. Cells in suspension vs. plated - (reply: 2)
     
320. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 4)
     
321. Hepg2 cells growing on top of each other - (reply: 1)
     
322. urgent! calculating concentration of compound with different cell amounts - (reply: 3)
     
323. h9c2 cells culture - (reply: 5)
     
324. Does anyone have experience of subjecting cultured cells to mechanical stress? - (reply: 1)
     
325. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
     
326. cell population doubling - (reply: 1)
     
327. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
     
328. Cell line licence - (reply: 1)
     
329. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
     
330. temperature tolerance of animal cells - (reply: 3)
     
331. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
     
332. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
     
333. Human insulin for cell culture? - (reply: 2)
     
334. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
     
335. Competent cells question: JM101 vs DH5alpha - (reply: 2)
     
336. Trouble producing stable cell lines - (reply: 2)
     
337. Infecting cells with virus - (reply: 1)
     
338. Requesting basic protocols on cell sorting - (reply: 1)
     
339. lysing mammalian cells in sds - (reply: 3)
     
340. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
     
341. Is it possible to assay the carbohydrate profile of a cell? - (reply: 2)
     
342. changing cell culture media - (reply: 1)
     
343. Preparing Palmitate:BSA solution for treatment of cells? - (reply: 7)
     
344. trypsinization of adherent cells - (reply: 9)
     
345. Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
     
346. Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
     
347. How to lysis cells for an RNA extraction - (reply: 1)
     
348. Problem with THP-1 cell line - (reply: 3)
     
349. normalization to protein content or cell number - (reply: 2)
     
350. detection of phosphorylation from cell pellet - (reply: 2)
     
351. are THP-1 Cells(Monocytes) or Macrophages resistant to Mycoplasma infection or - (reply: 4)
     
352. Recommended competent cell for transformation of large plasmids? - (reply: 4)
     
353. Cell culture shared with yeast? - (reply: 2)
     
354. E. coli cells refuse to grow! - (reply: 2)
     
355. how to fraction stem cell at different cell cycle stage - (reply: 2)
     
356. Making Nuclei From Mouse Cells - (reply: 1)
     
357. Different cell lines different protein stability - (reply: 1)
     
358. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
     
359. Immunofluorescence Staining of Cells - (reply: 1)
     
360. Nucleus fills the whole cell - (reply: 3)
     
361. Generating stable cell line with linear DNA - (reply: 3)
     
362. isolating cells from Cockroach - (reply: 2)
     
363. Question about suspension cells - (reply: 7)
     
364. Calu-3 cell line do not grow properly - (reply: 1)
     
365. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
     
366. Counting and plating cells - (reply: 3)
     
367. cells die 4-6 days after thawing - (reply: 8)
     
368. what is the trypsin X for subculturing - (reply: 1)
     
369. Cell Envelope Proteins Smeared - (reply: 1)
     
370. Calculating the amount of the desired cells in the medium ? - (reply: 3)
     
371. long term storage of cells at -80 - (reply: 1)
     
372. Trypan Blue Exclusion Test - (reply: 2)
     
373. Making BL21 competent cells - (reply: 2)
     
374. cell plating density - (reply: 2)
     
375. What kind of cell culture contamination is it? - (reply: 8)
     
376. Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
     
377. xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
     
378. to treat cells with inhibitor - (reply: 2)
     
379. lysis buffer not lysing adherent cells? - (reply: 8)
     
380. Trypan Blue Staining - (reply: 3)
     
381. Culturing cells on a slide - (reply: 5)
     
382. Culturing cells on a slide - (reply: 3)
     
383. protocol for immunostaining primery cells - (reply: 5)
     
384. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
     
385. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
     
386. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
     
387. Could the use of different FBS affact my cells - (reply: 5)
     
388. Analysis of high throughput screen for cell surface markers - (reply: 1)
     
389. Lymphoblastoid cell culture - (reply: 9)
     
390. SF9 Cells lose exponential growth, lack stationary phase - (reply: 3)
     
391. Don't know why my cells are unhealthy - (reply: 2)
     
392. Hybridoma cells not secreting antibody - (reply: 3)
     
393. Determining gene of interest from Cell culture lines - (reply: 2)
     
394. Three clones of a cell line? - (reply: 3)
     
395. Freezing cells in DMSO straight into minus 80? - (reply: 4)
     
396. MCF7 cells vitality - (reply: 3)
     
397. Ultrasonic bath for cell lysis - (reply: 3)
     
398. any methods to evaluate the attaching ability of suspension cells? - (reply: 6)
     
399. Vascular Smooth Muscle cell Marker - (reply: 3)
     
400. Foam in Cell Culture - (reply: 1)
     
401. Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
     
402. SURE cells - (reply: 1)
     
403. DT40 Cell Synchronisation - (reply: 2)
     
404. Bio-Rad Cell counter - (reply: 3)
     
405. SKBR-3 cells dying - (reply: 3)
     
406. New cell culture user with no teacher or trainer - (reply: 5)
     
407. Help with some problems with Culturing Epithelial Cells - (reply: 5)
     
408. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
     
409. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
     
410. What are these large multinucleated cells??? - (reply: 1)
     
411. Searching for a live cell staining method - (reply: 1)
     
412. WT BRAF/NRAS mouse Melanoma cells - (reply: 2)
     
413. HEK 293 cells Detachement - (reply: 8)
     
414. Cell culture in candle jar - (reply: 2)
     
415. centrifugation speed/cell prep for flow - (reply: 4)
     
416. Huge background with HT22 cells phophoERK antibody - (reply: 3)
     
417. Huge background with HT22 cells phophoERK antibody - (reply: 1)
     
418. Competency cells analysis - (reply: 1)
     
419. How many cells needed for co-IP and mass spec - (reply: 4)
     
420. Performing MTT assay in suspension cells (new in the subject) - (reply: 6)
     
421. cells die after transfection - (reply: 1)
     
422. Seeding cells on small metal screws - (reply: 1)
     
423. Cell lysate turbidity upon long storage - (reply: 3)
     
424. Host cells for transformation - (reply: 3)
     
425. Splitting or feeding cells for the weekend - (reply: 1)
     
426. Black moving particles in the cell Culture - (reply: 2)
     
427. lymphocyte proliferation using trypan blue - (reply: 2)
     
428. cell culture - (reply: 1)
     
429. cells shrink, detach from each other when changing media - (reply: 3)
     
430. Newbie help: Seeding cells to 6-well plates - (reply: 2)
     
431. 3t3 cell tured black - (reply: 9)
     
432. calculating the number of cells to freeze down - (reply: 2)
     
433. Fixed cells, sticky cells? - (reply: 2)
     
434. What is cotransfection in molecular cell biology? - (reply: 1)
     
435. BSA in adipocyte cellculture? Alternatives?? - (reply: 1)
     
436. serum deprivation of cell lines - (reply: 1)
     
437. serum deprivation of cell lines - (reply: 1)
     
438. What to do Post-transfection of cells with siRNA? - (reply: 2)
     
439. Problem with HEK stable cell line - (reply: 1)
     
440. HEK 293 cell culture - (reply: 3)
     
441. HepG2 cells resistant to Trypsinization - Please help!! - (reply: 8)
     
442. difficulty in passaging HK-2 cells - (reply: 5)
     
443. difficulty in passaging HK-2 cells - (reply: 2)
     
444. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
     
445. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
     
446. More cells in an antibiotic plate than in a plate without antibiotic???? - (reply: 2)
     
447. what are these cells?? - (reply: 1)
     
448. How to choose the right plasmids for certain types of cell? - (reply: 1)
     
449. Isolation of rat endothelial cells - (reply: 2)
     
450. protocol for making e.coli competent cells under low temperature - (reply: 1)
     
451. Looking for cell surface markers. - (reply: 3)
     
452. common nuclear marker for T cells - (reply: 1)
     
453. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
     
454. Any reliable markers for mouse memoery T cells? - (reply: 1)
     
455. Do mouse endothelial cells express the alphaVbeta3 integrin? - (reply: 1)
     
456. Cell Culture debris or contamination? - (reply: 4)
     
457. primary cell isolation from CNS, how to remove myeline debris - (reply: 1)
     
458. Platinum-A cell trasnfection toruble - (reply: 1)
     
459. cell culture media refrigeration - (reply: 4)
     
460. abnormal epithelial cells in mouse mammary gland - (reply: 1)
     
461. cell DNA content of cells grown on different scaffolds - (reply: 2)
     
462. Cancer cell starvation - (reply: 1)
     
463. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
     
464. Housekeeping gene for COS-1 cells - (reply: 1)
     
465. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
     
466. Monocytes die after cell scraping - (reply: 2)
     
467. The water pan got dry in the TC incubator. Will my cells be OK? - (reply: 1)
     
468. Cell fractionation - (reply: 2)
     
469. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
     
470. Dropped cells into liquid nitrogen tank - (reply: 3)
     
471. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
     
472. Small intestinal cell line for transport studies - (reply: 4)
     
473. help needed in determining volume of cells to be seeded - (reply: 6)
     
474. Dilution, Cell concentration & DNA content - (reply: 3)
     
475. competent cells - (reply: 2)
     
476. Cell lines & gene of interest - (reply: 1)
     
477. Software for selection genes related to concrete cell processes - (reply: 1)
     
478. Immunocytochemistry with HEK293 cells - (reply: 1)
     
479. How to determine apoptotic cell types in-vivo? - (reply: 1)
     
480. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
     
481. data analysis for single cell size and distribution - (reply: 1)
     
482. determining optimal cell count for MTT - (reply: 2)
     
483. Extracting DNA from FACS sorted cells - (reply: 3)
     
484. Storing frozen cells in -80 instead of liquid nitrogen - (reply: 5)
     
485. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
     
486. DG44 CHO cells and Methotrexate - (reply: 1)
     
487. extract plasmids from transfected eukaryotic cells - (reply: 6)
     
488. Trypsin Deactivation - (reply: 4)
     
489. Staining Cells in Suspension - (reply: 4)
     
490. cell culture confusion - (reply: 1)
     
491. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
     
492. chambered cell culture-slide for confocal microscopy - (reply: 3)
     
493. Nicotiana tabacum (BY-2) Suspension cells contaminated! Help! - (reply: 1)
     
494. urgh...mycoplasma drama..... - (reply: 5)
     
495. Dead or Alive... -_- (MCF-7 cells) - (reply: 7)
     
496. how to wash cells after fixation for a live binding study - (reply: 1)
     
497. Evenly distribute 100ul of cells in a 48/96 well - (reply: 2)
     
498. Cell lysis before shearing - (reply: 5)
     
499. really low puromycin concentration for selection of cells - (reply: 6)
     
500. Single stain control for 293 cell line - (reply: 4)
     
501. Is mCherry denatured by Trypsin or RIPA buffer? - (reply: 7)
     
502. loading dendritic cell with antigen - (reply: 1)
     
503. Cell Lysis and Western Blotting - (reply: 1)
     
504. problem with embryo carcinoma cell line , p19 , - (reply: 1)
     
505. In vitro induction of apoptosis in tumor cells by inactivated NDV and IAV - (reply: 1)
     
506. problem with embryo carcinoma cell line , p19 , - (reply: 1)
     
507. Recombinant protein from cell lysate - (reply: 4)
     
508. How to test if a compound is entering cells? - (reply: 3)
     
509. help with cell seeding - (reply: 2)
     
510. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
     
511. RNA isolation from frozen cells - (reply: 2)
     
512. How do you coat coverslips before seeding cells? - (reply: 3)
     
513. Buy.Ask for buying some cell lines and antibodies - (reply: 3)
     
514. competentce cell, cloning - (reply: 2)
     
515. Flp-recombinase target site integration in 293 T-REx cells - (reply: 1)
     
516. Storage of transfected PC12 cells - (reply: 1)
     
517. cell adhension - (reply: 1)
     
518. Using Methanol For Cell Lysis - (reply: 1)
     
519. Adjusting pH of cell culture medium for ELISA - (reply: 1)
     
520. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
     
521. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
     
522. Contamination of primary cells with fibroblast. - (reply: 1)
     
523. Help With Lysing HEK293 & Fibroblast Cells - (reply: 1)
     
524. problem with siRNA transfection of HEK cells - (reply: 5)
     
525. Splitting A549 cells - (reply: 9)
     
526. NMDA Transfection in 293 Cells - (reply: 1)
     
527. freezing adherant cells - (reply: 2)
     
528. freezing adherant cells - (reply: 2)
     
529. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
     
530. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
     
531. cells stably expressing T7 RNA polymerase - (reply: 1)
     
532. Cell Fixation - Experience with cell fixation? (reply: 5)
     
533. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
     
534. Is pCL-Eco suitable for packaging viruses that used to infect human cells? - (reply: 1)
     
535. Cause of Death for Cell Culture? - (reply: 5)
     
536. creat a SY5Y stable cell line - (reply: 1)
     
537. selecting drug-resistant colon cancer cells - (reply: 2)
     
538. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
     
539. Helper T cell skewing - (reply: 3)
     
540. Optimum serum starvation period for NIH, CHO, COS7 cells - (reply: 4)
     
541. Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
     
542. competent cells stock - (reply: 3)
     
543. intracellular cytokine staining - re-stimulation of activated T cells (reply: 1)
     
544. DH5alpha cells - (reply: 4)
     
545. pheonix cells and &293A cells - (reply: 1)
     
546. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
     
547. Protein purification from HEK 293 cells - (reply: 1)
     
548. Growing hybridoma cells in medium without serum - (reply: 2)
     
549. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
     
550. Cell drawing tools? - (reply: 6)
     
551. How many cells in plant embryos ? - seed biology (reply: 1)
     
552. cell count - (reply: 8)
     
553. question on making chemical and electrocompetent cells - (reply: 2)
     
554. COS7 cells giving me problems - (reply: 1)
     
555. Where to get 5T series cells? - (reply: 1)
     
556. cell number for colony formation - HEK293, NIH3T3 (reply: 1)
     
557. Electroporation/electrotransfection mammalian cells - (reply: 7)
     
558. Cell debris here, there, everywhere!? - (reply: 2)
     
559. Cell debris here, there, everywhere!? - (reply: 1)
     
560. Mini prep of frozen bacteria cells - (reply: 2)
     
561. Help! Turned on UV light. Did I kill my cells? - (reply: 2)
     
562. growing competent cells - (reply: 1)
     
563. Freezing density for NIH 3T3 cells - (reply: 3)
     
564. Stable Cell Line generation - (reply: 3)
     
565. making more competent cells - (reply: 2)
     
566. CellTracker dye in necrotic cells? - (reply: 1)
     
567. The case of the punky Sf9 cells - (reply: 2)
     
568. sticky perm cells (tween 20)- can I prevent it? - (reply: 2)
     
569. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
     
570. collecting cells HT1080 - (reply: 4)
     
571. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
     
572. Help: problem with MDCK cells - (reply: 2)
     
573. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
     
574. Oh my cell culture problems never cease!!! - (reply: 4)
     
575. WEHI-3BD cell culturing - Advice on culturing. (reply: 5)
     
576. cell homogenization - (reply: 13)
     
577. cell count - (reply: 3)
     
578. cell lysis - confocal slides (reply: 4)
     
579. HeLa cell anatomy question - (reply: 3)
     
580. Need cell lines wich express separately TLR 2, 4 and 5 - (reply: 1)
     
581. MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
     
582. THP-1 cell line - (reply: 3)
     
583. CaMKII alpha promoter and cell tranfection - (reply: 1)
     
584. Adaptation of mammalian cells to serum free mode - (reply: 2)
     
585. Live cell Imaging medium - (reply: 1)
     
586. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
     
587. MDA-MB-231 cell transfection - (reply: 4)
     
588. Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
     
589. Break Cell Clumps - prepare single cells for injections (reply: 1)
     
590. Stem Cells Sphere - stem cells (reply: 2)
     
591. Request for cell lines? - (reply: 1)
     
592. Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
     
593. MTT problems with Huh7 cell line, plzzzzzzz help - MTT color problem (reply: 5)
     
594. Calculating cell doubling time - (reply: 1)
     
595. SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
     
596. Infected cells that are dying after puromycin selection - (reply: 1)
     
597. contamination issue in cell culture - (reply: 1)
     
598. Why do cells need to be washed in PBS before biotinylation? - (reply: 3)
     
599. AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD (reply: 2)
     
600. Purification of recombinant proteins from insect cells - (reply: 2)
     
601. Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
     
602. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
     
603. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
     
604. Growth curve for PC3 cell line - (reply: 1)
     
605. Agrobacterium EHA101 competent cells - (reply: 1)
     
606. THP-1 cell line - (reply: 2)
     
607. Effective DCFDA Protocol for Non-Adherent THP-1 Cells? - (reply: 1)
     
608. Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
     
609. best freezing method for electrocompetent cells - (reply: 9)
     
610. cell seeding calculation - (reply: 8)
     
611. Improved Stem Cell Proliferation - Would interested in improving proliferation of adult stem cells? (reply: 5)
     
612. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
     
613. Yeast contamination in cell culture - (reply: 8)
     
614. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
     
615. Remove cells from dish and fix them in slides - (reply: 3)
     
616. Help- lysing cells with triton x-100 - (reply: 1)
     
617. E.coli cells lyse in minimal media - (reply: 3)
     
618. How stable are suspension cells in room temperature? - (reply: 2)
     
619. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
     
620. Transfection in insect cell lines?? - (reply: 1)
     
621. PCR genomic DNA using cell lysate - (reply: 1)
     
622. HELA Cells Capable of producing whole, infective HPV particles? - (reply: 3)
     
623. Distilled water for Electrocompetent Cell Preparation - (reply: 6)
     
624. Should I fix cells digested from tissues? - (reply: 4)
     
625. STR cell line identification - looking for cell line identification method (reply: 1)
     
626. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
     
627. Rat Dental Pulp Stem Cell Culture - (reply: 1)
     
628. NK cell analysis using CD16PE and CD56PE - (reply: 2)
     
629. My neuroblastoma are floating!! please help - Both SK-NSH and SH-SY5Y are adherent cells, but are only floating now (reply: 3)
     
630. cell suspencion and medium - (reply: 5)
     
631. One Shot TOP10 Cells - (reply: 6)
     
632. trypan blue for chlorella - (reply: 1)
     
633. Help on BmN4 cell culture - How to maintain BmN4 cells? (reply: 1)
     
634. Compare ChIP to cell lysate - (reply: 1)
     
635. Compare ChIP to cell lysate - (reply: 1)
     
636. Testing cytoxixity on healthy human cells - (reply: 2)
     
637. what cell line to use - to demonstrate ubiquitination (reply: 1)
     
638. GFP cells intracellular staining - (reply: 1)
     
639. Xenograph - how to maintain cell viability? - (reply: 1)
     
640. Western blot of apoptotic cells - (reply: 2)
     
641. cell sycle and proliferation - (reply: 3)
     
642. help with cell dilution - (reply: 2)
     
643. Difficult Cell Proliferation Experiment - (reply: 2)
     
644. Cell dilution cloning - Cells cloning (reply: 2)
     
645. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
     
646. HTC116 cells detaching !!!! - (reply: 2)
     
647. Revival of kasumi-1 cells - (reply: 3)
     
648. HEK 293 Trypsin vs. only EDTA - (reply: 2)
     
649. Growth conditions for E. coli DB3.1 cells - (reply: 1)
     
650. Mycoplasma cleanup time... - (reply: 10)
     
651. weird & curious problem with CHO cell culture - (reply: 3)
     
652. HaCat do not grow after using cell scraper - (reply: 5)
     
653. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
     
654. Cell Lysates from a Western Blog - (reply: 2)
     
655. Loss of YFP signal in transduced T cells - (reply: 7)
     
656. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
     
657. Freezing cell pellets and minimizing lysis - (reply: 2)
     
658. Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells? (reply: 6)
     
659. Total Cell protein - (reply: 1)
     
660. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
     
661. Mycoplasma - (reply: 1)
     
662. cell culture media - glutamine! - (reply: 2)
     
663. How many cells do you have in a confluent T 75 flask? - I have 1/2 million (reply: 1)
     
664. how to make antigen by trypsin and papain digetion - plz teel me hoe to make antigen by trypsin & papain digetion (reply: 1)
     
665. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
     
666. Premature cell lysis - (reply: 2)
     
667. urgent!!! weird Growth in cell line - (reply: 8)
     
668. refrozen cell pellet - (reply: 1)
     
669. Drug vehicle giving toxicity in cell culture - With increse in vehicle..... toxicity decrese (reply: 4)
     
670. contamination in cell line...... - (reply: 4)
     
671. Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 7)
     
672. Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
     
673. MTT with cells growhing in suspension, not adherent - (reply: 3)
     
674. Cell ELISA - No differences between cells expressing and not, the receptor (reply: 2)
     
675. growth curve for attached cells - (reply: 1)
     
676. cells dont attach hard enough to 96 well - (reply: 3)
     
677. CTLL-2 culture - preventing cell clumping - (reply: 2)
     
678. Yet another contamination question - SF9 cells (reply: 5)
     
679. Problems with transfection in human cells - (reply: 3)
     
680. HepG2 cells culture conditions - (reply: 2)
     
681. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
     
682. T cell proliferation problem - (reply: 2)
     
683. How to Handle Cells Shipped Internationally in Dry Ice? - (reply: 2)
     
684. Differences between cells in different species - (reply: 5)
     
685. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
     
686. isotype control for GFP expressing cells? - (reply: 2)
     
687. Trypsin for J774A Macrophages? - (reply: 2)
     
688. Trouble with growth of transformed cells - (reply: 4)
     
689. nuclear extraction of blood cells - (reply: 2)
     
690. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
     
691. ELISA with undiluted cell lysate samples - (reply: 1)
     
692. How can you expand cells if you have one dish of them? - (reply: 1)
     
693. Androgen independent LNCaP cells - (reply: 2)
     
694. mycoplasma detection kit - (reply: 1)
     
695. What is the proper way to infect cell culture with virus? - (reply: 5)
     
696. Micro-RNA profiling for differentiating cells - (reply: 1)
     
697. Growing insect cells with other cell lines - (reply: 2)
     
698. Candida albicans cells lysis - method (reply: 1)
     
699. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
     
700. Advise for Whole Cell Stain - hESC-differentiated Trophoblast (reply: 1)
     
701. 293 T cells - (reply: 3)
     
702. Mouse Bone Marrow Isolation - How to increase yield of BM-derived cells? (reply: 1)
     
703. BL-21 Gold (DE3) cells and further strains - (reply: 2)
     
704. Cell Cycle Analysis flow cytometry - How to measure desynchronisation rate of cells (reply: 1)
     
705. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
     
706. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
     
707. Fixing cells before staining of surface markers - (reply: 1)
     
708. Black dots in cell culture - (reply: 7)
     
709. cell counting - (reply: 5)
     
710. Optimization for cell viability assay (MTT assay) - (reply: 1)
     
711. Anyone thought about reprogram fibroblast to liver cells? - (reply: 2)
     
712. cell confluence in cell cultures - myoblast c2c12 in particular (reply: 2)
     
713. cell line adherence - cells adhering to flask very tightly after transfectiono (reply: 1)
     
714. Cell labelling - How can I label 2 cell populations? (no transgenes) (reply: 1)
     
715. How long does it take for cells to reattach? - From balls to pancakes. (reply: 3)
     
716. Dot plot cell cycle analysis-strange dots - (reply: 4)
     
717. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
     
718. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
     
719. help Splashed Raji cells on skin/face/hand - (reply: 2)
     
720. Help! ES Lenti infection efficiency=nightmare - trouble getting over 40 % cells infected (reply: 8)
     
721. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
     
722. CO2 level for cell culture - (reply: 6)
     
723. sphere and suspension culture subculturing - (reply: 1)
     
724. suspension cells for confocal - (reply: 1)
     
725. RNAi is somatic and germline cells - (reply: 3)
     
726. Luciferase and cell responsiveness - (reply: 3)
     
727. adult cell culture - (reply: 1)
     
728. protein overexpression in HeLa cells - (reply: 1)
     
729. Insulin supplements to cell culture media - (reply: 1)
     
730. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
     
731. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
     
732. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
     
733. topic and cell culture - (reply: 2)
     
734. Running a western with cell media in gels? - (reply: 11)
     
735. Starting cells for making one's own competent cells - (reply: 7)
     
736. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
     
737. Persistent stable cell line (Bos23) Contamination... - (reply: 2)
     
738. Hematopoietic cells separation - How to get high purity and viability hematopoietic cell populations? (reply: 2)
     
739. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
     
740. min6 cells immunofourescence staining - staining and culture problem (reply: 4)
     
741. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
     
742. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
     
743. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
     
744. rat ES cells - (reply: 2)
     
745. Mycoplasma contamination - (reply: 1)
     
746. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
     
747. establishment of primary cell line from biopsy - (reply: 2)
     
748. Cell culture fasting - (reply: 1)
     
749. mutated HeLa cells? - from cycloheximide? (reply: 1)
     
750. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
     
751. qPCR for transformed cells - (reply: 1)
     
752. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
     
753. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
     
754. descrepancy between total cell lysate and cell fractionation - (reply: 1)
     
755. Disruption of cells using mild detergent - (reply: 2)
     
756. Eruption of mammalian cells using detergent - (reply: 1)
     
757. lenti virus transfected cell dies... - (reply: 4)
     
758. Extremely Weird Issue - CHO Cell Culture (reply: 2)
     
759. Cell Die.. Need help - (reply: 1)
     
760. Cell scraping ruining my life - (reply: 6)
     
761. How much trypsin/Cause of death - (reply: 3)
     
762. Death of cells in the control. - (reply: 1)
     
763. cell culture clarification - I need help please (reply: 2)
     
764. Serum concentration in treatment of cells for MTT assay - (reply: 1)
     
765. Minimum number of cells needed for cytometry? - (reply: 3)
     
766. Beta-Lactam in CHO Cells - (reply: 1)
     
767. problems with 293T cells after medium removal!!! - (reply: 2)
     
768. B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
     
769. Propagating ccdB survival cells - (reply: 5)
     
770. Western difficulty in primary neuron cells. - (reply: 1)
     
771. DOubt - Competent cell (reply: 2)
     
772. Differentiation of CD11b cells into macrophages.Help please - (reply: 1)
     
773. Please Help!! - Mycoplasma (reply: 5)
     
774. Primary keratinocyte isolation- cell loss - (reply: 1)
     
775. cells attach but don't flatten out - MDA-231 (reply: 8)
     
776. Problem Generating Stable HeLa Cells - (reply: 1)
     
777. Retroviral transduction of T cells - (reply: 4)
     
778. Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
     
779. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
     
780. Multiple nuclei?? - cell morphology - FaDu (reply: 8)
     
781. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
     
782. how to compensate the cell death during transfection by electroporation - (reply: 2)
     
783. H-ras transformed RK3E/tv-a cells - (reply: 1)
     
784. HEK001 Cell Line and Papillomavirus - Does HEK001 cell line produce a functional papillomavirus (reply: 4)
     
785. 2 questions: myeloma cell lines and mice MABs - (reply: 5)
     
786. I'm getting fried-egg looking cells... What should I do?? - (reply: 8)
     
787. Empty bubbles in the 3T3-L1 cells - (reply: 3)
     
788. Exposing Cell Lines to Drugs Metabolized by Liver Microsomes - (reply: 1)
     
789. UV safety in cell culture hood - (reply: 3)
     
790. Where can I find info on the physical appearance of TB cell wall structures? - I am an artist wanting to depict the cell wall with absolute accuracy. (reply: 7)
     
791. intracelluar staining without killing cells - (reply: 3)
     
792. Info about A7r5 cell culture - (reply: 1)
     
793. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
     
794. Cell lysates - Protein precipitation? - (reply: 3)
     
795. Glucose in epithelial cell - (reply: 1)
     
796. cell lysate for pulldown - (reply: 3)
     
797. cell culture media - (reply: 3)
     
798. Competent cell storage for long term use - (reply: 2)
     
799. My RIPA cell lysis buffer - Western blot (reply: 3)
     
800. Cell Uptake Study - (reply: 1)
     
801. what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
     
802. aspects of RAW264.7 cells - (reply: 5)
     
803. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
     
804. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
     
805. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
     
806. Hoechst Stain does it stain dead cells - (reply: 3)
     
807. Mycoplasma detection - (reply: 2)
     
808. Help for cell preparation for FACS... - (reply: 1)
     
809. Cell Lysis Buffer - (reply: 6)
     
810. HeLa cells and HPV - (reply: 2)
     
811. Resolving cell growth problems using growth additives - way to resolve urgent cell growth problems (reply: 3)
     
812. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
     
813. problem with MACS cell isolation - (reply: 5)
     
814. cell culture split ratios. - (reply: 2)
     
815. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
     
816. detection of mycoplasma with hoechst staining - (reply: 1)
     
817. blood red cells - (reply: 1)
     
818. Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
     
819. NFKb signalling in cell line ??? - (reply: 2)
     
820. kit fpr cell membrane preparation? - (reply: 2)
     
821. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
     
822. How to find NFKb signalling is active in a cell line??? - (reply: 2)
     
823. Fixing YFP cells - (reply: 2)
     
824. MTT Assay: cell proliferation or cell viability??? - (reply: 4)
     
825. Rescue RAW cell culture - RAW cells past confluent point... can they be rescued? (reply: 4)
     
826. cell cycle arrest by colcemid - (reply: 2)
     
827. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
     
828. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
     
829. PROLIFERATION ASSAY IN MICROTITER PLATES WITHOUT A SEMIAUTOMATIC CELL HARVESTER - (reply: 1)
     
830. THP cells? what does THP stands for?????????? - (reply: 1)
     
831. Immunioprecipitation from Jurkat cells?? - (reply: 1)
     
832. Weird effect of siRNA on protein in cells - (reply: 3)
     
833. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
     
834. EDTA addition to trypsin solution (1x) - (reply: 1)
     
835. Apoptosis induction - for vero and mcf-7 cells (reply: 5)
     
836. Need pH 6 Cell culture media - (reply: 1)
     
837. Are my cells undergoing epigenetic changes? - (reply: 2)
     
838. establishment of stable cell line using hek293t - (reply: 2)
     
839. Cell pellet frozen -80 then -20 - (reply: 4)
     
840. total cell protein vs membrane protein - (reply: 4)
     
841. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
     
842. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
     
843. Fixing the cells for FACS - Paraformaldehyde or Formaldehyde (reply: 2)
     
844. Problem with control samples in cell IC - (reply: 3)
     
845. Recommendation for cell cycle analysis software needed - (reply: 1)
     
846. Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
     
847. Cell culture contamination question - what the hell are these things?? - (reply: 3)
     
848. 'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
     
849. Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
     
850. Immunofluorescence using dead cells? - (reply: 1)
     
851. floating THP-1 cells - medium change advice - (reply: 2)
     
852. my cells love infection, any thoughts on antibiotics? - (reply: 3)
     
853. Culture Media for stem cells - (reply: 1)
     
854. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
     
855. Anyone heard of cells spontaneously fluorescing red?? - (reply: 4)
     
856. Freezing cells - How long can I keep cells at -80C? (reply: 6)
     
857. Help regarding cell line - (reply: 1)
     
858. organizing cell lines - (reply: 1)
     
859. Cell plating density - (reply: 3)
     
860. Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
     
861. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
     
862. MDA-MB-231 cells question - (reply: 3)
     
863. thawed cells wont adhere - pc3 and LNcaP (reply: 2)
     
864. DiFi Cell line (colon carcinome) - Where can I get it???? (reply: 1)
     
865. HaCaT cells - (reply: 2)
     
866. lncap and MCF-7become ethanol sensitive? - are lncap cells and MCF-7 cells ethanol-sensitive? (reply: 2)
     
867. Dying endometrial stromal cells - (reply: 1)
     
868. Help me - Are my cells infected with something or could this be unsusual morphol - (reply: 5)
     
869. how to dissociate cells for cell migration assay - (reply: 3)
     
870. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
     
871. RNA extraction - how many cells do I need at least?? (reply: 4)
     
872. RNA extraction protocol for small amounts of cells - (reply: 2)
     
873. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
     
874. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
     
875. strange morphology of sp2/0 cells after fusion!!! Please help! - morphology switch from round to triangular!!!! (reply: 2)
     
876. fabrablast primery cell line problem - how to detach cell well (reply: 1)
     
877. what solvent is better compatible for most cell lines? - DMSO vs methanol (reply: 3)
     
878. Cell growth rate & O2 consumption - (reply: 2)
     
879. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
     
880. Cells stick to dish in Embryoid Bodies - (reply: 1)
     
881. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
     
882. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
     
883. how to plate cells precisely - (reply: 1)
     
884. RNA extraction from stained cells - (reply: 2)
     
885. Methods to assess cell viability - (reply: 1)
     
886. suspension cell transient transfection - (reply: 9)
     
887. Adherent cells washing off in 96 well -MTT - (reply: 4)
     
888. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
     
889. plasmids - plasmids for stable cell line construction (reply: 2)
     
890. abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
     
891. average total protein content per 293t cell - (reply: 2)
     
892. T cells preparation for FACS - (reply: 1)
     
893. counting cells - (reply: 3)
     
894. Cell OD and spec problems - (reply: 1)
     
895. cell confluency & effect on cells...?? - (reply: 4)
     
896. Pipet trypsin tips - (reply: 3)
     
897. Fixing cells for flow cytometry - alternative to 1% paraformaldehyde (reply: 3)
     
898. mycoplasma false positive - Luminescence false positives for myco (reply: 2)
     
899. Washing Red Blood Cells - Tips on washing red blood cells (reply: 7)
     
900. MEF Cell marker - (reply: 1)
     
901. (CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
     
902. Change of colour in cell culture media...? - (reply: 2)
     
903. cell count - how to calculate (reply: 2)
     
904. generating cell line knockouts - (reply: 3)
     
905. Cancer Stem Cells?? - (reply: 10)
     
906. Some kind of contamination? - mouse transduced cell line (reply: 7)
     
907. Cell stimulation of transcription - (reply: 5)
     
908. Mouse blood density/osmolarity of blood cells for separation - (reply: 5)
     
909. suitable cell lysis buffer - (reply: 1)
     
910. Toluidine blue staining for cell culture - (reply: 1)
     
911. cell culture - 3D cell culture (reply: 1)
     
912. Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
     
913. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
     
914. MEF culture contamination? - Contaminated cell culture? (reply: 2)
     
915. very simple question about hemocytometer cell counting - (reply: 1)
     
916. nucleic acid extraction from silenced cells - (reply: 6)
     
917. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
     
918. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
     
919. In Vitro Drug Exposure Cytotoxicity Testing and Cell Confluency - (reply: 8)
     
920. Dendritic cells - Protocol of stimulation of dendritic cells (reply: 3)
     
921. giant cells in HEK293 cell line - (reply: 10)
     
922. Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
     
923. Plant cell transfection - (reply: 1)
     
924. stable cell line - selection after retroviral infection (reply: 1)
     
925. Storing cell lines long-term at -70C - (reply: 4)
     
926. Commercially available primary mouse endothelial cell line? - (reply: 1)
     
927. Frozen cells in -80 - (reply: 1)
     
928. Plant or Fungal Cell Dissociation Technique? - (reply: 3)
     
929. how can i bring cells onto a slide? - ...for microscopical observation! (reply: 7)
     
930. cell density to be seeded? - cytotoxicity studies (reply: 1)
     
931. Lysis of the cells - (reply: 1)
     
932. Cells do not lyse in SDS buffer - (reply: 1)
     
933. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
     
934. lysis buffer for lymphoblast cells - (reply: 1)
     
935. problem with cell cycle analysis - (reply: 3)
     
936. THP-1 Cells differentiation - THP-1 cells differentiated using IFN-gamma/LPS (reply: 7)
     
937. Single cell suspension from solid tumor - (reply: 3)
     
938. T-Cell receptor - Is it a single type on a T cell or multiple types? (reply: 1)
     
939. cells weak adhesion - detachment (reply: 2)
     
940. how we count the passages in cell cultures? - (reply: 5)
     
941. resolving cell growth problems - (reply: 2)
     
942. clumping of cells - (reply: 7)
     
943. stability of cell suspension - (reply: 1)
     
944. cell adhesion in serum free condition - (reply: 1)
     
945. effects of cell confluency - (reply: 1)
     
946. Which trypsin EDTA to use? - (reply: 1)
     
947. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
     
948. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
     
949. polarized cells - (reply: 4)
     
950. PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
     
951. cell adhesion assay - using collagen (reply: 1)
     
952. Problem clariflying/filtering insect cell lysate - (reply: 2)
     
953. cell culture urgent!! - (reply: 2)
     
954. Moving S2 cells from one plate to another - (reply: 1)
     
955. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
     
956. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
     
957. mononuclear cells medium - I am stuck with the concentration of 2-mercaptoethanol (reply: 1)
     
958. Accidental thawing of frozen cells - (reply: 3)
     
959. how to start with a new cell line ? - how to start with a new cell line ? (reply: 1)
     
960. HaCaT cells die after trypsinization - (reply: 1)
     
961. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
     
962. GFP and Luciferase Cell Line - (reply: 2)
     
963. Transfecting primary cells with plasmid - (reply: 1)
     
964. transfecting and selecting EL4 cells - (reply: 1)
     
965. Induce Osteoblast lineage from C2C12 myoblast cells - (reply: 1)
     
966. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
     
967. Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
     
968. Transfection into eukaryotic cells - (reply: 1)
     
969. Fixing cells with PFA - (reply: 1)
     
970. A549 cells not doubling - has anyone been through this with other cell lines? (reply: 4)
     
971. Contamination in SH-SY5Y cultures, mycoplasma? - (reply: 2)
     
972. Immunofluorescence in HEK cells issues - (reply: 1)
     
973. cell sorting - losing cells? - (reply: 3)
     
974. What if my expressed protein is toxic for the cells... - (reply: 1)
     
975. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
     
976. Possible contamination of leukemic cell culture - debris, growth inhibition, cell aggregates (reply: 3)
     
977. human cancer cell line splashed into my eyes - (reply: 3)
     
978. Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
     
979. BL21(DE3)LysS competent cells - (reply: 1)
     
980. ATP turnover in neuron - ATP hydrolysis rates in neuronal cells (reply: 2)
     
981. Adhering CHO cell culture - cells don't adhere! (reply: 2)
     
982. do e.coli cells get ruptured during high spin - (reply: 1)
     
983. Please Help with COS-1 cells^^ - (reply: 1)
     
984. antibiotics for preventation of mycoplasma contamination - (reply: 4)
     
985. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
     
986. cell counting - to get an impression when my cells are in the log phase (reply: 1)
     
987. Whole cell lysis of fibroblasts - (reply: 1)
     
988. Whole cell lysis of fibroblasts - (reply: 1)
     
989. test of cytotoxic effect of compounds - why cells are pretreated with actinomycin C ?? (reply: 1)
     
990. Proliferation Assay - Should cells be in the logarithmic phase ? (reply: 3)
     
991. cell density and growth - (reply: 5)
     
992. Laser capture microdissection - capturing single cells (reply: 2)
     
993. when cells have to be synchronized?? - (reply: 2)
     
994. cells in RNAlater - the title say`s it all :) (reply: 4)
     
995. Cytotoxicity assay for adherent cells exposed to particles - (reply: 1)
     
996. Murine Cell Lines - (reply: 2)
     
997. Cell Proliferation: To adhere or not to adhere (before treatment), that is the q - (reply: 2)
     
998. why are some cell lines more popular than others?? - (reply: 4)
     
999. NK cell staining - (reply: 3)
     
1000. cell culture media - with or w/o L-glutamine? (reply: 2)
      
1001. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
      
1002. pro-apoptotic effect of compounds on cancer cell lines - which cell line to test the non-toxic effect? (reply: 3)
      
1003. Expiry of Trypan blue - (reply: 3)
      
1004. Question in Generating double stable cell line - (reply: 3)
      
1005. activity of sex-chromosome in somatic cells - (reply: 5)
      
1006. CaCl2 for making competent cells - (reply: 3)
      
1007. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
      
1008. ICC Cell Adherence Issue - (reply: 1)
      
1009. gentamycin resistance in human cencer cell culture - please suggest the alternative antibiotic (reply: 2)
      
1010. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
      
1011. How to normalize my samples before I transfect my cells - (reply: 6)
      
1012. Isolation of intestinal epithelial cells - Isolation of intestinal epithelial cells (reply: 2)
      
1013. Number of proteins expressed in cell - (reply: 2)
      
1014. Do HEK 293 cells contain functional E1A/E1B? - (reply: 2)
      
1015. Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
      
1016. Calcium phosphate method vs lipofectamine - troublemaker neuroblastoma cells (reply: 7)
      
1017. Which cell lines are best for transient transgene expression? - (reply: 3)
      
1018. Alzheimer's cell model - (reply: 7)
      
1019. Partial cell subculture? - (reply: 2)
      
1020. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
      
1021. Whole Kidney primary cell culture - (reply: 1)
      
1022. dh5 alpha competent cells? - (reply: 2)
      
1023. which is the best lysis buffer for whole cell extracts? - (reply: 1)
      
1024. Please help me to understand- cell counting - (reply: 1)
      
1025. Stimulating hepatocytes with IL-1beta - (reply: 1)
      
1026. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
      
1027. Cell count - Please help - (reply: 11)
      
1028. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
      
1029. Cell Count - (reply: 1)
      
1030. Generating stable cell line - (reply: 2)
      
1031. Harvesting cells off of collagen gels - (reply: 1)
      
1032. cells can't be lysed after crosslinking - (reply: 4)
      
1033. How many cells to seed? - simple question :) (reply: 5)
      
1034. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
      
1035. mouse macrophage cell line - (reply: 5)
      
1036. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
      
1037. cell line information - require (reply: 1)
      
1038. Urgent problem - Media change = cells die in 48h (reply: 3)
      
1039. recombineering for ES cell targeting - retrieval problems (reply: 1)
      
1040. How often do you thaw new cells? - passage number or time? (reply: 7)
      
1041. Does high speed centrifugation destroy viable cells? - (reply: 2)
      
1042. Cell culture - (reply: 7)
      
1043. Problems when plating neuroblastoma cells - (reply: 3)
      
1044. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
      
1045. Cell Doubling Time - (reply: 3)
      
1046. Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
      
1047. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
      
1048. synchronized cells or non-synchronized cells - (reply: 3)
      
1049. pre-prepararation of competent cells - (reply: 3)
      
1050. ROS accumulation - ROS reactive oxygen species accumulation in cells (reply: 3)
      
1051. Bacterial invasion assay with human cells - (reply: 2)
      
1052. Measuring gene expression across cell lines - (reply: 2)
      
1053. trypan blue without hemocytometer? - (reply: 2)
      
1054. How to make an isotonic solution for h9c2 cells - (reply: 2)
      
1055. migration < invasion??? - More cells migrating through matrigel than through control inserts (reply: 1)
      
1056. Zeocin of G418. Which is the better selection agent for CHO cells? - (reply: 1)
      
1057. Immunofluorescence with transfected cells - (reply: 3)
      
1058. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
      
1059. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
      
1060. Contamination in cell cultures - (reply: 9)
      
1061. DMSO killing more cells at lower concentrations - (reply: 1)
      
1062. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
      
1063. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
      
1064. lysing cells on ice - (reply: 2)
      
1065. Fibroblast cell culture - (reply: 1)
      
1066. Cardiac stem cells - (reply: 3)
      
1067. How to visualize tumor cells in mice with out sacrificing the animals - (reply: 3)
      
1068. cell count and dilution - (reply: 1)
      
1069. z competent cells - (reply: 2)
      
1070. immunofluorescence staining of sperm cells - (reply: 2)
      
1071. Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
      
1072. colony formation assay - how many cells to seed (reply: 2)
      
1073. Primaria/gelatin plates and gravity-defying fixed cells - (reply: 2)
      
1074. Contaminations in cells cultured in suspension - (reply: 2)
      
1075. suspension cell transient transfection - (reply: 2)
      
1076. What is this ? - Cell culture contaminant (reply: 2)
      
1077. cell cryopreservation after siRNA transfection - (reply: 1)
      
1078. Cell Digestion Protocol - (reply: 1)
      
1079. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
      
1080. cell growth - (reply: 4)
      
1081. primary cells could not attch to regular polystyrene flasks but immortalised cel - does anybody know what could be happening (reply: 1)
      
1082. How to culture cells after sorting? - (reply: 3)
      
1083. How to kill nerve cells in a living hydrozoans? - Trying to procude partial/complete nerveless jellyfish (reply: 3)
      
1084. lifespan of primary cells in vitro - how long can different cell types survive (reply: 3)
      
1085. measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
      
1086. T cell differentiation protocols - Th1/Th2/Treg/Th17 (reply: 4)
      
1087. Mitochondria extraction from human liver cells - (reply: 1)
      
1088. lipofectamine and PEI transfection of hela cells - (reply: 2)
      
1089. What can I do whit my Cancer Stem Cells?(I know, its a big question..) - (reply: 7)
      
1090. Wash L929 Cells with DPBS (with or w/out CA MG?) - DPBS with or W/out CA,MG? (reply: 2)
      
1091. Especially tenacious cells -- what to do? - (reply: 9)
      
1092. MDCK cell vascuolation - I got my MDCK cell growing in the T-75 flask (reply: 5)
      
1093. Culturing HeLa Cells - (reply: 2)
      
1094. expression of His-tagged protein in insect cells - (reply: 2)
      
1095. How to isolate vascular smooth muscle cell? - (reply: 1)
      
1096. Whole cell lysate including nucelar proteins? - (reply: 3)
      
1097. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
      
1098. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
      
1099. culturing MCF-7 cellls - clumping and maintaining healthy cells (reply: 3)
      
1100. Cell culture media - require information on media (reply: 3)
      
1101. how to measure ROS in cell wiht H2DCFH - (reply: 4)
      
1102. VEGF in cell nucleus - (reply: 2)
      
1103. cell treatment rules - (reply: 4)
      
1104. method to seed cells in hyrogels - cell seed in hydrogels (reply: 1)
      
1105. transfecting human mesynchemal stem cells - (reply: 5)
      
1106. cell schock - (reply: 2)
      
1107. Flow cytometry of RFP and CFP expressing yeast cells - (reply: 1)
      
1108. Can you store cell pellets for future nuclear extraction? - (reply: 5)
      
1109. using cell lysate to run native gel? - (reply: 1)
      
1110. Cell Suspension from Frozen Tissue? - (reply: 6)
      
1111. looking for stromal cell marker - (reply: 1)
      
1112. 'Black dots' in cell culture - (reply: 2)
      
1113. ChIP in WT and KO cells - how to analyze - (reply: 1)
      
1114. Mouse T cell line - (reply: 1)
      
1115. Making competent cell from freeze-thawed stock - (reply: 2)
      
1116. Invasion assay using matrigel- cell clumps - (reply: 1)
      
1117. producing cell lines - why viruses ?? (reply: 4)
      
1118. What color should the cell pellets be after sonication and centrifugation? - (reply: 1)
      
1119. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
      
1120. Mycoplasma Question - (reply: 2)
      
1121. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
      
1122. How to detach an antibody from cell surface - (reply: 4)
      
1123. Water for electrocompetent cells - (reply: 2)
      
1124. Gp 293 Luc cells - (reply: 1)
      
1125. how to check cell viability - (reply: 1)
      
1126. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
      
1127. cell fixation - (reply: 1)
      
1128. cell detachment in IHC - can I incubate cells with hoechst, heparin and PFA at the same time? (reply: 1)
      
1129. cell immobilization for confocal - (reply: 1)
      
1130. problem with 293T cell co-transfection - (reply: 5)
      
1131. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
      
1132. Cell Death after formaldehyde treatment - Immunofluorescence (reply: 5)
      
1133. Cell Densitometry - (reply: 1)
      
1134. My cells died after changing media... - (reply: 6)
      
1135. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
      
1136. H9C2 cells - trouble when culturing H9C2 cells (reply: 4)
      
1137. Contamination of cell ?? - (reply: 2)
      
1138. Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
      
1139. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
      
1140. Passaging Cells - (reply: 4)
      
1141. Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
      
1142. Mysterious cell death - need Hela Help! - (reply: 1)
      
1143. NSC-34 Cells sloughing off at media change - (reply: 4)
      
1144. Chosing the right competent cells - (reply: 1)
      
1145. Cell proliferation assay for LNCaP cells - (reply: 4)
      
1146. liquid nitrogen in cell culture - (reply: 2)
      
1147. differential cell counting in mixed cultures - (reply: 1)
      
1148. Questions about animal cell cultue Please answer - (reply: 1)
      
1149. immunofluorescence for HUVEC - how to do immunofluorescence for cancer cells (reply: 3)
      
1150. membrane type of cell culture filtration - (reply: 3)
      
1151. Critical column of media above cells for access of oxygen - (reply: 1)
      
1152. EDTA bacterial cell lysate - (reply: 2)
      
1153. Is MTS Assay suitable for Adherent cells? - Confused about MTT Assay and MTS assay.. (reply: 1)
      
1154. membrane damaged = cell death? - (reply: 2)
      
1155. 293 cells help! - (reply: 1)
      
1156. stable cell line - (reply: 2)
      
1157. Color of cryopreserved cell line - (reply: 1)
      
1158. Established (tumor) cell line - or not...? - (reply: 3)
      
1159. Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 1)
      
1160. proliferation assay for small cell population - (reply: 2)
      
1161. Cell fixation with glutaraldehyde - (reply: 2)
      
1162. Yeast contamination in cell culture - hood to blame? - (reply: 6)
      
1163. Comparing cell # or DNA content - of pelleted cells (reply: 1)
      
1164. bicarbonate in cell culture: powder v. liquid? - (reply: 3)
      
1165. cell adhesion assay - collagen (reply: 1)
      
1166. To break cell clumps - suspension culture related (reply: 4)
      
1167. Problems for MTT in SH-SY5Y cell line - (reply: 1)
      
1168. cell stainer size - (reply: 2)
      
1169. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
      
1170. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
      
1171. Low concentration of FBS inhibiting trypsin? - (reply: 1)
      
1172. contamination in establishing stable cell lines - (reply: 1)
      
1173. How much cell medium for 1 week of culturing? - (reply: 1)
      
1174. p53 +/- matched-pair tumor cell lines? - (reply: 2)
      
1175. Tranfection cell density and overgrowth - (reply: 3)
      
1176. shRNA selection in suspension cell culture - (reply: 6)
      
1177. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
      
1178. Ethanol/methanol/dmso for cell culture - (reply: 1)
      
1179. stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
      
1180. Rapid Cell Count (in vivo) - (reply: 2)
      
1181. cell migration assay (corning transwell) - (reply: 2)
      
1182. cell culture in 2 stack flask - (reply: 1)
      
1183. Invitogen cell countess - (reply: 5)
      
1184. Hypoxia Treatment For Cell Culture - (reply: 4)
      
1185. Picking strain for e coli competent cells - (reply: 6)
      
1186. cell death - can anybody tell me what process this looks like (reply: 3)
      
1187. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
      
1188. Marker do discriminate cell cycle phases - G2 phase / Mitosis (reply: 3)
      
1189. hematopoietic cell lines - (reply: 3)
      
1190. Cos7 Cells to produce retrovirus - (reply: 2)
      
1191. how to make nonadhensive cells attach to coverslips without affecting cellular s - (reply: 1)
      
1192. Constructing puromycin kill curve - How to count the number of adherent cells without detaching them? (reply: 3)
      
1193. stable cell line generation - (reply: 5)
      
1194. histological staining for plant cell wall components - (reply: 1)
      
1195. My adherent cells are detaching - (reply: 2)
      
1196. Cell culture media - DMEM and DMEM/F-12 (reply: 1)
      
1197. Cell Cycle analysis of proliferating lymphocytes - (reply: 1)
      
1198. Problems with HEK293 cells in 96 well plate format - 293 cells do not adhere well to the plate (reply: 1)
      
1199. Cell Synchronization - (reply: 1)
      
1200. Slow cell culture growth? - (reply: 3)
      
1201. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
      
1202. cell lysis buffer - (reply: 1)
      
1203. cell aging - cell culture (reply: 2)
      
1204. cell concentrations - (reply: 4)
      
1205. Floating cells...pls help - (reply: 6)
      
1206. Cell migration assay with 3 cell types - (reply: 1)
      
1207. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
      
1208. How many B cells do I need to get a 1mg of protien - (reply: 2)
      
1209. Do histology staningings work in cell culture? - (reply: 2)
      
1210. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
      
1211. Trypsin Activity Assay - No Change (reply: 4)
      
1212. T cell transduction - (reply: 3)
      
1213. compound that block DNA replication in human cell - (reply: 1)
      
1214. Tranfection of HL 60 cells - (reply: 1)
      
1215. mouse liver cell line? - (reply: 1)
      
1216. Failed Transformation of Tuner (DE3) cells - (reply: 1)
      
1217. Cells suddenly died after changing the medium... why? - (reply: 4)
      
1218. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
      
1219. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
      
1220. CHO Cell contamination - (reply: 6)
      
1221. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
      
1222. seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 1)
      
1223. Neuronal precursor cells - (reply: 1)
      
1224. Sticky cells - (reply: 2)
      
1225. Having problem establishing stable cell line...... - (reply: 5)
      
1226. Transferring mamallian cell colonies for selection - (reply: 2)
      
1227. Antibody titration and Cell number - (reply: 1)
      
1228. Cell Invasion Assay - possible to analyze with flow cytometry? (reply: 5)
      
1229. Cell culture contamination - "outsider I.D." (reply: 2)
      
1230. Alternative media for PC-12 cells - (reply: 2)
      
1231. viability of adherent cells - (reply: 3)
      
1232. How long can viably frozen cells stay good in -80 degree? - (reply: 5)
      
1233. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
      
1234. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
      
1235. HeLa cells and BCL 2 expression - (reply: 1)
      
1236. B104 cells - (reply: 2)
      
1237. SH-SYS5 cell culture for dummies... - (reply: 4)
      
1238. collagen staining in cell culture - (reply: 5)
      
1239. Hematopoietic lineage depletion kit - Hematopoietic stem cell enrichment kits (reply: 4)
      
1240. changing cell shape - (reply: 2)
      
1241. how to change cell shape? - (reply: 3)
      
1242. About T-antigen and tumor cell lines - (reply: 2)
      
1243. Sub-culture of N2a cells - (reply: 1)
      
1244. Haematopoietic stem cells - (reply: 5)
      
1245. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
      
1246. proteomics - minimum cell number (reply: 1)
      
1247. Standard Curve for OD Turbidity vs Cell density - (reply: 6)
      
1248. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
      
1249. Plasmids for protein expression in mammalian cells - (reply: 2)
      
1250. cell lysis after induction - (reply: 1)
      
1251. competent cell preparation? - (reply: 1)
      
1252. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
      
1253. Dendritic cell infection - (reply: 2)
      
1254. Competent Cells - (reply: 5)
      
1255. looking for bovine skeletal muscle cells - (reply: 5)
      
1256. my cell line doesnt change the colour of the media - even when cells are 100 % confluent (reply: 4)
      
1257. Dead Dendritic cells ... please help - (reply: 1)
      
1258. Cell concentration Question - (reply: 3)
      
1259. cho cell line adoptation to serum free medium - (reply: 1)
      
1260. Problems with viability of Jurkat's cells - (reply: 3)
      
1261. THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells (reply: 1)
      
1262. 24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
      
1263. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
      
1264. Culturing U2OS cells - (reply: 1)
      
1265. staph cell count - (reply: 5)
      
1266. What kind of speed for HepG2 cells. - (reply: 2)
      
1267. Serum free culture media 293T cells? - (reply: 1)
      
1268. Embryoid bodies - How to differentiate mouse ES cells quickly and efficiently (reply: 8)
      
1269. cell count - is it right? (reply: 3)
      
1270. Haemocytometer cell count - Coverslips (reply: 4)
      
1271. Growing two cell types in the same flask?? - (reply: 1)
      
1272. Cell isolation - (reply: 1)
      
1273. trouble to grow cells on top of coverslip - (reply: 6)
      
1274. Immortalized murine hepatocytes - (reply: 1)
      
1275. about measuring luciferase activity in living cells - (reply: 8)
      
1276. Culture conditions for cell treatments - (reply: 4)
      
1277. preadipocyte 3T3-L1 cells question? - (reply: 1)
      
1278. SH-SY5Y cell culture - Suspended cells (reply: 4)
      
1279. Magnetic-activated cell sorting ? - (reply: 4)
      
1280. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
      
1281. How to increase IF signal in acetone/methanol fixed cells? - (reply: 5)
      
1282. Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
      
1283. sheep red blood cell rosetting protocol - (reply: 1)
      
1284. Tranformation cell harvesting - (reply: 5)
      
1285. self-renewal Vs proliferation - stem cells (reply: 3)
      
1286. Plyse cell and DH5a - (reply: 1)
      
1287. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
      
1288. INS-1 cell line - (reply: 1)
      
1289. Central cell seeding - (reply: 4)
      
1290. please help with Freezing primary cells - (reply: 1)
      
1291. COMPETENT CELL PREPARATION - (reply: 6)
      
1292. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
      
1293. suggest cells expressing low levels of adhesion molecules - (reply: 1)
      
1294. Cell Culture and Pipette Tips - (reply: 4)
      
1295. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
      
1296. Cell counting issues - the ring of death on my flasks (reply: 5)
      
1297. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
      
1298. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
      
1299. RNA Isolation - RNA isolation from human cell lines (reply: 2)
      
1300. Cell Fixation - methanol fixation of cells (reply: 3)
      
1301. How to retrieve my human blood immune cells from liquid nitrogen without killing - (reply: 3)
      
1302. stem cells and EB (Embryoid bodies) - (reply: 1)
      
1303. Immunohistchemistry of red blood cells - (reply: 1)
      
1304. cell cryosection - (reply: 5)
      
1305. Shifting of entire cell cycle to the left - (reply: 3)
      
1306. freezing suspended cells - (reply: 7)
      
1307. Cell Brakeage...how will I know - I need the mitochondria. (reply: 1)
      
1308. How many cells... - ...gives you how much RNA? (reply: 2)
      
1309. Cell culture - Culture medium (reply: 1)
      
1310. Anybody ever use whole blood plasma serum stimulate cells? - (reply: 2)
      
1311. How can I separate my cell line from feeder cells - (reply: 3)
      
1312. ChIP-seq: Minimum number of cells - (reply: 3)
      
1313. CHO or VERO? - Cell culture (reply: 2)
      
1314. Media for squamous cells - (reply: 3)
      
1315. how to trace single cell movement throughout gastrulation of zebrafish - (reply: 4)
      
1316. Oxygen diffusion through cell culture medium - (reply: 2)
      
1317. Request for Mono Mac-1 cell line - (reply: 1)
      
1318. help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
      
1319. How much sonication power needed? - protein extraction from cells (reply: 2)
      
1320. protein extraction - from cell line in T25 flask (reply: 5)
      
1321. Getting cells from flasks - (reply: 5)
      
1322. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
      
1323. Fixation of immune cells - "how to" protocol needed (reply: 2)
      
1324. cell cycle analysis by FACS - (reply: 2)
      
1325. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
      
1326. Trypsin Expression and Purification - (reply: 3)
      
1327. cell cycle analysis PI staining shift - (reply: 1)
      
1328. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
      
1329. Problem with stable overexpression cell lines - (reply: 3)
      
1330. seeking help in stable cell line generation - (reply: 3)
      
1331. SDS, Cell numbers, dilution - (reply: 18)
      
1332. Help with Hep G2 cells - (reply: 1)
      
1333. Floating cells in adherent VERO culture - contamination? (reply: 2)
      
1334. RAW264.7 cells+nitric oxide determination - (reply: 13)
      
1335. BL 21 cells - (reply: 4)
      
1336. Mutated MDCK cells - protocol for mutating MDCK cells (reply: 1)
      
1337. Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
      
1338. Cell pellet pesuspension without Vortexing or pippeting - seeking advice - (reply: 2)
      
1339. western blot problem: cell culture VS mouse tissue - (reply: 4)
      
1340. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 2)
      
1341. Detachment of very adherent cells for flow cytometry - (reply: 7)
      
1342. THP-1-Blue-CD14 cells - (reply: 4)
      
1343. are cells prone to swelling in PBS after sorting - (reply: 3)
      
1344. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
      
1345. suspension cell culture - optimum cell number in plates (reply: 2)
      
1346. Whole tissue starting product - not cultured cells - (reply: 2)
      
1347. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
      
1348. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
      
1349. Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
      
1350. culturing COS-7 cells - (reply: 5)
      
1351. Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
      
1352. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
      
1353. Loss of cell adherence after transfection - The first passage after selection process cells lost adherence (reply: 2)
      
1354. how will i be able to culture tumor cells? - (reply: 3)
      
1355. General cell lysis question - whole cell lysates need to be centrifuged? (reply: 2)
      
1356. Rnase for cell cycle - (reply: 5)
      
1357. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
      
1358. cell loss upon centrifugatino - (reply: 10)
      
1359. cell lines vs tissue expression - (reply: 1)
      
1360. Is molecular biology grade DMSO good enough for cell stock? - (reply: 2)
      
1361. Clonogenic assay with agressive cell line - (reply: 4)
      
1362. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
      
1363. Number of cells in 6 wells-plate well - (reply: 1)
      
1364. protein extraction from primary cells - (reply: 1)
      
1365. Extracting proteins from cells - (reply: 1)
      
1366. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
      
1367. strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 4)
      
1368. cell floating in 96-well plate - (reply: 1)
      
1369. Inducing Adherence in Suspension Cells - (reply: 1)
      
1370. Isolated nuclei and trypan blue - Silly question .......... (reply: 2)
      
1371. bacterial contamination in cell culture - (reply: 3)
      
1372. THP-1 cell culture - (reply: 1)
      
1373. H9c2 cell contamination? - (reply: 4)
      
1374. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
      
1375. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
      
1376. What stains red in trypan blue - (reply: 2)
      
1377. transfection in floating cells - (reply: 4)
      
1378. immortal or stable cells - primary human cells to immortal cell line? (reply: 3)
      
1379. SDS-PAGE Cell lysis problems - (reply: 3)
      
1380. Storage of invitrogen top10 cells - (reply: 11)
      
1381. oxidative stress - how to measure oxidative stress in cells (reply: 2)
      
1382. Cells clots forming during preparation mouse splenocytes - (reply: 4)
      
1383. Transwell Cell Migration/Invasion Assay - (reply: 4)
      
1384. neuronal nitric oxide synthase/cell line - (reply: 3)
      
1385. HL 60 cells passage - (reply: 1)
      
1386. Establishing a new cell line - (reply: 1)
      
1387. Isolating Natural Killer cells from whole blood - (reply: 2)
      
1388. Centrifugation of epithelial cells - (reply: 2)
      
1389. cell engineering exam - (reply: 5)
      
1390. Cell detachment: alternative to trypsine/EDTA? - (reply: 3)
      
1391. Cell supplier - (reply: 2)
      
1392. Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
      
1393. Mir34a positive control cells - (reply: 4)
      
1394. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
      
1395. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
      
1396. FACS positive cells in negative controls help - (reply: 1)
      
1397. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
      
1398. How to separate fungus cells from human lung tissues - (reply: 7)
      
1399. Growing of TOP10 chemically competent cells - (reply: 4)
      
1400. Stable Cell Selection with Neomycin - (reply: 4)
      
1401. endogenous miRNA of cos-7 cell - (reply: 2)
      
1402. Qustion about MDA-MB-157 cells culture - (reply: 2)
      
1403. Folic acid solution for cell culture - (reply: 2)
      
1404. Mycoplasma contamination - (reply: 6)
      
1405. Strange dots around the cell+Photo - (reply: 6)
      
1406. FACS of GFP expressing cells from mice brains - (reply: 1)
      
1407. cells can't attach in the flasks - (reply: 4)
      
1408. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
      
1409. Cell fractionation and isolation of nuclei - (reply: 1)
      
1410. Methanol toxicity in cell culture - (reply: 15)
      
1411. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
      
1412. Freezing cells, quick question - (reply: 1)
      
1413. Best place to buy cells - (reply: 2)
      
1414. help on generation of stable cell lines with pBabe vector - pBabe-puro, phoenix Ampho cells (reply: 7)
      
1415. DMSO usage in cell culture - (reply: 9)
      
1416. stopping brief cell treatments - (reply: 2)
      
1417. Phenol red in media - What metabolic products of cells are acidic? (reply: 2)
      
1418. Cell culture container - (reply: 2)
      
1419. cell lysis method - (reply: 1)
      
1420. Dendritic cell immunology - activated naive cells? (reply: 9)
      
1421. cell contamination???? - (reply: 1)
      
1422. counting number of cells - (reply: 6)
      
1423. Stacking of RAW 264.7 cells - (reply: 1)
      
1424. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
      
1425. Lentiviral transduction of B cells - (reply: 7)
      
1426. Lentivirus transduction of spleen cells - (reply: 2)
      
1427. trouble with establishing a stable cell line - (reply: 3)
      
1428. Cell culture conditions and antigen expression - (reply: 1)
      
1429. Phoenix packaging cell and diphtheria toxin - (reply: 3)
      
1430. Gene expression in cell line - (reply: 1)
      
1431. isoflurane and FACS analysis of peripheral blood? - my cells look terrible everytime I use isoflurane to anaesthetize (reply: 5)
      
1432. Growing Up Cells - (reply: 3)
      
1433. protein extraction after cell lysing - (reply: 2)
      
1434. cell line for neurodevelopment - (reply: 2)
      
1435. Difficulty in doing hemocytometer cell count - (reply: 4)
      
1436. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
      
1437. Method for lysing cells for fractionation - (reply: 4)
      
1438. Neuronal cell culture - why use neonatal brains? - (reply: 1)
      
1439. Detect gene in stable cell line, - (reply: 4)
      
1440. staining cells - (reply: 3)
      
1441. pIRESneo for mammalian cells transfection - (reply: 4)
      
1442. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
      
1443. H9C2 - cell line - Need clarification (reply: 1)
      
1444. Cell plating calculation !! - Help needed with Cell plating calculation !! (reply: 2)
      
1445. Cell lines to study epigenetics - (reply: 2)
      
1446. How to transfect two plasmids in a cell - (reply: 10)
      
1447. Cells dying before i can get them to in vitro assay - (reply: 1)
      
1448. Strange bands in western of RPE-1 cells - (reply: 1)
      
1449. Semisolid Medium For HL 60 cells - (reply: 4)
      
1450. Cell culture contamination (do not know what it was) - (reply: 1)
      
1451. questions about cell lysis - (reply: 7)
      
1452. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
      
1453. How to homogenize cell without disruption of nuclei? - (reply: 2)
      
1454. Harvesting cells for flow cytometry - (reply: 10)
      
1455. culture C6/36 insect cell lines - (reply: 1)
      
1456. seeding cell densities - seeding cells at same density (reply: 1)
      
1457. cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
      
1458. Culturing QT6 cell line - Ideal culture conditions (reply: 2)
      
1459. Calculating MOI for luciferase-transducted cells. - Would the sensitivity of a fluorescent microscope be enough to see the (reply: 3)
      
1460. Picking epithelial, endothelial and neural cell lines - Please suggest best models (reply: 2)
      
1461. How to fix suspencion cells for DAPI detecting under the microscope? - (reply: 6)
      
1462. mycoplasma growth - (reply: 3)
      
1463. primary lung epithelial cells - (reply: 1)
      
1464. Generating double stable cell line - (reply: 3)
      
1465. HACAT cells - very low cell viability after thawing cells (reply: 18)
      
1466. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
      
1467. My cells suddenly die !!! - Please help me ! why is this ? (reply: 11)
      
1468. database for mutations in cancer cell lines? - (reply: 2)
      
1469. Sonication Problems, Branson 450, LNCaP cells - (reply: 4)
      
1470. ? Definition of Passage for suspension cells - (reply: 1)
      
1471. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
      
1472. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
      
1473. Chip problem: antibody or cell number?? - (reply: 6)
      
1474. stable cell line - (reply: 1)
      
1475. microbial cell viability - any recommendations - assays (reply: 1)
      
1476. SF9 cells not adhering - (reply: 10)
      
1477. HeLa cells - Growth of thawed HeLa cells (reply: 7)
      
1478. Dendritic Cells and Flow Cytometry - (reply: 11)
      
1479. direct cell lysate - (reply: 8)
      
1480. How long can I wait after seeing HeLa cells till transfection - will it affect my Efficiency (reply: 6)
      
1481. Culturing/Mounting of polarised cells for confocal microscopy - (reply: 1)
      
1482. Intestinal cell culture: Protocol required - (reply: 1)
      
1483. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
      
1484. Carbon di oxide free cell culture - (reply: 1)
      
1485. surface staining of THP-1 cell - (reply: 2)
      
1486. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
      
1487. Cell Wall Lysis For Dummies - (reply: 4)
      
1488. Tissue & Cell Fixation solutions - (reply: 3)
      
1489. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
      
1490. Treating cells with chemicals and how to dilute into medium - (reply: 5)
      
1491. T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (reply: 6)
      
1492. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
      
1493. Has anyone used COS-7 cells for retina? - (reply: 1)
      
1494. How to perform a growth curve? - i need to compare 3 cell lines for growth (reply: 2)
      
1495. siRNA concentration in divinding-growing cells - (reply: 2)
      
1496. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
      
1497. cell culture guideline from abcam - (reply: 5)
      
1498. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
      
1499. problems with detecting protein expression - protein expression in cell (reply: 4)
      
1500. freeze PBMC and cell sorting? - (reply: 1)
      
1501. Control vehicle treatment in cells - (reply: 2)
      
1502. Cancer cell culture exploded onto my face - (reply: 8)
      
1503. FACS analysis of GFP expressing cells - Fix or not - (reply: 10)
      
1504. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
      
1505. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
      
1506. Heterogeneous methylation in a same cell line - (reply: 1)
      
1507. siRNA cell function examples - (reply: 1)
      
1508. Good comprehensive resource for maintaining immortal cell lines? - (reply: 1)
      
1509. DH5a comp cells - (reply: 5)
      
1510. Trypsinized cells after fixation - (reply: 3)
      
1511. Detaching Hela cells - (reply: 3)
      
1512. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
      
1513. cell cycle softawe - (reply: 3)
      
1514. How to maintain cells at a specific apoptosis stage - (reply: 12)
      
1515. Which cell line produce cytokine? - (reply: 13)
      
1516. Cell Viability - (reply: 1)
      
1517. Shipping of cryopreseved cell - (reply: 15)
      
1518. To what extent competent cells be thawed before transformation? - (reply: 4)
      
1519. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
      
1520. Storing HEK293 cells at -80C - (reply: 5)
      
1521. How to control cloned cDNA expression level in cells? - (reply: 8)
      
1522. Asses mycoplasma contamination - (reply: 6)
      
1523. RNA isolation following apoptotic cell labeling - (reply: 3)
      
1524. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
      
1525. Is there an optimal incubation time for cell lysis? - (reply: 7)
      
1526. The way to count adherent cells in 96 well - (reply: 3)
      
1527. lysing cells in LSB - (reply: 2)
      
1528. Using NIH3T3 cells of look at MAPK activation by Ras - (reply: 1)
      
1529. Staining for neural crest derived cells?? - (reply: 2)
      
1530. Dendritic_ cells - (reply: 3)
      
1531. problem to stain just one cell line in coculture - (reply: 4)
      
1532. Inactivation of trypsin - (reply: 4)
      
1533. Culturing stromal cells - (reply: 2)
      
1534. A549 cells - (reply: 4)
      
1535. Cunterstaining Trypan blue - (reply: 6)
      
1536. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
      
1537. Protein extraction from cell culture media - (reply: 9)
      
1538. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
      
1539. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
      
1540. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
      
1541. can we prepare Copmetent cell by calicum cloride with 0.09 OD - Cp cells with 0.09 OD (reply: 3)
      
1542. electroporation of HL60 cells - (reply: 1)
      
1543. Intestinal epithelial cell culture - (reply: 4)
      
1544. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
      
1545. Easiest cell lysis protocol - no interest in preserving proteines (reply: 4)
      
1546. passage number for cell line - (reply: 7)
      
1547. MOCK INFECTED CELLS - (reply: 4)
      
1548. cuboidal cells - (reply: 1)
      
1549. Cell count using a hemocytometer - (reply: 4)
      
1550. Hepatocyte culture - (reply: 4)
      
1551. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
      
1552. what is mock cell line? - (reply: 4)
      
1553. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
      
1554. How to seed the cells evenly in 6 wells plate - (reply: 13)
      
1555. knockout mouse with increase cell size - (reply: 3)
      
1556. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
      
1557. Paper on energy requirement of cells - (reply: 2)
      
1558. galactose induction of yeast cells - (reply: 2)
      
1559. making competent E coli DH5a cells - (reply: 6)
      
1560. How could you explain these results? - cancer cells are weird (reply: 1)
      
1561. strange ES cells contamination - please help!!! (reply: 1)
      
1562. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)
      
1563. How to culture PC3 cell line - (reply: 3)
      

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