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Cell culture Related Discussions
  1. Subculturing colonies to agar - (reply: 2)
  2. Why my bacteria cells look weird in DAPI fluorescence? - (reply: 2)
  3. lentiviral transduction in BLCL cells - (reply: 3)
  4. Synchronization of HEK293T cells for IP - (reply: 1)
  5. Alternate cell line not giving same results as first - (reply: 1)
  6. Cell volume and reciprocal cell volume? Whaaat? - (reply: 1)
  7. How to detect specific-protein producing cells? - (reply: 8)
  8. Who came up with 50-100 IU/mL Antibiotic working concentration in cell culture? - (reply: 2)
  9. Help! Antibiotic and Serum Concentration in Cell Culture Media - (reply: 1)
  10. Large-scale cell growth and protein expression - (reply: 1)
  11. is cell signaling difficult? - (reply: 3)
  12. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
  13. The adipose cell culturing - (reply: 1)
  14. Recovering cells from ancient sample - (reply: 3)
  15. Microinjection of cells into the lumen of spheroids ? - (reply: 1)
  16. Human cell membrane protein Western blot! - (reply: 2)
  17. Cell Line confluence level - (reply: 1)
  18. too much cell density can influence adhesion to culture plate - (reply: 1)
  19. Shipment conditions for cultured cells - (reply: 3)
  20. E-Cadherin Expression in OAW-42 Cells - (reply: 1)
  21. Why HepG2 cells looked like that? - (reply: 2)
  22. Trouble transfecting HEK 293 T and HUH 7 cells - (reply: 1)
  23. 3T3-L1 cell contamination - (reply: 3)
  24. Will lipofection of DNA work in post mitotic cells - (reply: 1)
  25. Cell death determination - (reply: 2)
  26. Culture Tubes for CHO cells - (reply: 1)
  27. SHSH5Y cells - (reply: 1)
  28. Is the mycoplasma that infects cell culture dangerous to humans? - (reply: 1)
  29. Distinguishing two populations of cells by TEM - (reply: 4)
  30. transfection of suspended CHO cells - (reply: 2)
  31. using IC50 to determine dosing of inhibitor in cell culture - (reply: 2)
  32. Software for automated cell counting? - (reply: 2)
  33. why is my cells dying in the centre of the wells after transfections - (reply: 1)
  34. How to establish drug-resistant cell lines? - (reply: 1)
  35. Are normal or immortalized human epithelial cells clonogenic? - (reply: 1)
  36. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
  37. Fibroblast Cell Culture Issue - Cells grouping together and cell clusters observ - (reply: 3)
  38. pUC and pSC101 vectors in the same cell - (reply: 3)
  39. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
  40. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
  41. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
  42. Is cultured cell reuse possible? - (reply: 1)
  43. Count cell bactria using haemacytometer - (reply: 1)
  44. Help with HUVEC cell transfection - (reply: 3)
  45. Cell clumps or contamination? Please help! - (reply: 1)
  46. Is any other method to maintain without changing media, or store cell culture fo - (reply: 2)
  47. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  48. Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
  49. Abnormality in PMA treated THP1 cells - (reply: 1)
  50. Preferred assay for measuring cytotoxicity or cell proliferation - (reply: 2)
  51. Cell lysis - (reply: 1)
  52. Can foam created when resuspending a cell pellet cause cell demage - (reply: 1)
  53. Distribution of cell receptors on the cell surface - (reply: 3)
  54. MTT Cell Treatment - (reply: 1)
  55. cells behaving differently - (reply: 4)
  56. Cell lines behaving differently to drugs - (reply: 2)
  57. How to achieve a uniform monolayer with HepG2 cells, seeded in 24 welll plate fo - (reply: 1)
  58. Efficient lysis of E.coli cells - (reply: 4)
  59. Alternative competent cells for QuikChange kit - (reply: 2)
  60. unknown bodies in cell culture - (reply: 1)
  61. Unbroken cell - (reply: 1)
  62. how to confirm lentiviral integration in a target cell line - (reply: 1)
  63. Invasion assay: analysis question: round cells - (reply: 2)
  64. Measuring ammonia in cell lysates - (reply: 1)
  65. Methanol oor PFA to fix cells for nuclear protien analysis by immunofluorescence - (reply: 4)
  66. What happens when trypsin is added to cells? - (reply: 1)
  67. Selective evolution of cells for cancer - (reply: 4)
  68. cell line - (reply: 3)
  69. I want know what companies are producing Biosimilar cell line - (reply: 1)
  70. contamination in HepG2 cell culture - (reply: 1)
  71. Which neuroblastoma cell lines grow the longest axons - (reply: 1)
  72. Help with Question for Cell Bio Take Home Exam - (reply: 2)
  73. leukemic cell lines 697 - (reply: 1)
  74. where do you get cells needed for your research - (reply: 1)
  75. PCR with mammalian cells as template - (reply: 1)
  76. Dislodge cell in 96well plate - (reply: 1)
  77. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  78. cell counting - (reply: 2)
  79. What do the glioma cell line prefixes LN and U stand for ? - (reply: 1)
  80. Trypan Blue Staining - (reply: 1)
  81. colorectal cell culture - (reply: 2)
  82. Easy to transfect primary cell line? - (reply: 1)
  83. Sample prep of cells for FACS analysis and running WB - (reply: 4)
  84. Functional states of the same cell versus Different cell types - (reply: 2)
  85. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
  86. transfection of cells with two fluorescent reporters - (reply: 1)
  87. Stably transfected cells start dying - (reply: 6)
  88. U251 and U87 cells - (reply: 1)
  89. is -40 freezer enough for cell freezing? - (reply: 1)
  90. Experiment on nutrient surplus on Jurkat cell culture - (reply: 2)
  91. Help with HeLa cell cultivation - (reply: 5)
  92. skin lesion and low donor cell frequency in bone marrow transfer - (reply: 7)
  93. cell division rates - (reply: 6)
  94. Black dots-ghost cells or contamination? Please help - (reply: 6)
  95. Where do you purchase T cell hybridomas - (reply: 1)
  96. NK Cell Culture Selection and Expansion from PBMC - (reply: 2)
  97. 3D Cell Culture Matrix made of jelly / gelatin - (reply: 2)
  98. Cloning purified PCR product into cells - (reply: 5)
  99. Help ID fluoescently-labelled cells in the gut very much appreciated! - (reply: 2)
  100. Mention of Transgene Insertion into Cell Lines By Transfecting with cDNA - (reply: 2)
  101. Refreeze thawed cells? - (reply: 3)
  102. How can an animal cell obtain needed glucose when glucose is lacking in the diet - (reply: 2)
  103. Suppose a microorganism invades a cell, causing damage to ATP synthase. How woul - (reply: 3)
  104. How to capture live cells under microscope - (reply: 3)
  105. Identification of cells and tissue - (reply: 2)
  106. Research topic related to jurkat cell line - (reply: 3)
  107. What DCH5a cells are? / pCAMBIA1301 isolation from DHC5a cells - (reply: 3)
  108. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
  109. Assays from very few number of cells - (reply: 1)
  110. What I have to do If my active compound showing toxicity in normal cells? - (reply: 1)
  111. Passaging cells - (reply: 1)
  112. Problem in mouse embryonic stem cell culture - (reply: 4)
  113. Is there always a presence of activated T cells in a healthy host? - (reply: 1)
  114. Bacterial contamination in cell culture - (reply: 3)
  115. Cell culture contamination - (reply: 1)
  116. Contamination or dead cells? - (reply: 1)
  117. How to choose cell line - (reply: 4)
  118. Urea effect on mice spleen cells - (reply: 3)
  119. Urea effect on mice spleen cells - (reply: 1)
  120. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
  121. Best cryovials for liquid nitrogen storage of cells? - (reply: 2)
  122. Review articles on all types of mammalian cell culture - Any suggestion? - (reply: 1)
  123. 2 types of cell lines for RNA extraction - (reply: 1)
  124. live cell culture transport in 6 well plate - (reply: 6)
  125. Stem Cell Culture Theory - (reply: 4)
  126. Selectively arresting a subpopulation of biotinylated yeast cells. - (reply: 4)
  127. cell viability with PI staining - (reply: 2)
  128. trypsin digestion of 2D-PAGE protein spot - (reply: 2)
  129. Sonication of cells in ultrasound bath, without probe - (reply: 3)
  130. cell fractionation and microscopy - (reply: 3)
  131. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
  132. cell lysis problem - (reply: 2)
  133. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
  134. Thawing cells - (reply: 2)
  135. the same construct, two stable cell clones with different protein size - (reply: 2)
  136. Determine DNA sequence expression in cell line - (reply: 1)
  137. Harvesting cells - (reply: 3)
  138. Cell counters - Beckman Coulter Z1 counter - (reply: 1)
  139. Competent cells with Hanahan's method - (reply: 2)
  140. problem with cell synchronization - (reply: 4)
  141. can anyone tell me what kind of cell this is? - (reply: 3)
  142. Transfection of SH-SY5Y cells - (reply: 4)
  143. Protocol for isolating cells from Subcutaneous Tumors ? - (reply: 1)
  144. Hybridoma cells contaminated with large round cells! - (reply: 1)
  145. protocol for cell syncronization using colchicine - (reply: 2)
  146. PBMC cell stimulation? - (reply: 1)
  147. Caco-2 cells with unidentified contaminant - (reply: 2)
  148. MIN6 Cell Culture Contamination Risk - (reply: 1)
  149. Concentrating bacterial cells - (reply: 2)
  150. Would preserved cells for flow cytometry make your life easier? - (reply: 1)
  151. measure of cell size - (reply: 1)
  152. Transfecting cells for cell cycle analysis, how to deal with cell density? - (reply: 1)
  153. Splitting Cells that are shaped as small spheres - (reply: 2)
  154. Cells counting problem. - (reply: 2)
  155. Cell counts for Clonogenic and MTT assay - (reply: 3)
  156. Do I need to use untreated TC flask for suspension cell culture - (reply: 5)
  157. Cell cycle determination - (reply: 1)
  158. After centrifuge in serum-free media, forming cell sheet, instead of pellet in t - (reply: 2)
  159. Can cells be exposed to nanoparticles with ethanol as solvent??? - (reply: 2)
  160. Question about growth media for A20 Cell Line - (reply: 1)
  161. NSO cell culture - (reply: 1)
  162. Some queries regarding A549 cell culture - (reply: 1)
  163. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
  164. how to transport stem cells in suspension? - (reply: 2)
  165. animal cell culture - (reply: 1)
  166. help with contamination of cell culture - (reply: 2)
  167. Methanol concentration for cells? - (reply: 4)
  168. What happened to my cell? (Colony formation) - (reply: 7)
  169. problem of cell lysate - (reply: 3)
  170. Cell lysis and DNA quantification - (reply: 1)
  171. Best western loading control for comparing different cell lines - (reply: 2)
  172. B cell line - cell colour change and visible cell death - (reply: 1)
  173. Keeping apoptotic/detached cells for WB? - (reply: 3)
  174. Effective inhibitor of cell proliferation - (reply: 4)
  175. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
  176. Neural Stem Cells; does it mean adult or embryonic? - (reply: 1)
  177. Cell culture contamination or not? - (reply: 1)
  178. orthotopic mouse model of U251 cell line - (reply: 4)
  179. How to release the bacterial cells from filter paper? - (reply: 4)
  180. Cell adhesion/detachment - (reply: 1)
  181. methylation observation in normal cell line - (reply: 1)
  182. Stem cells created by dipping researchers into coffee - (reply: 2)
  183. Freezing yeast cells - (reply: 4)
  184. codon optimization for CHO cells - (reply: 2)
  185. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  186. chk effect of cell line genes on normal dna - (reply: 1)
  187. Would someone help me discuss what work can I do with laryngeal cancer cell hep2 - (reply: 12)
  188. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
  189. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
  190. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
  191. CHIP on frozen cell pellet - (reply: 1)
  192. UV Light benefits and costs on Cells - (reply: 2)
  193. Adapting a Drosophila cell culture to Zebrafish for subsequent MACS-sorting - (reply: 3)
  194. mouse embryonic stem cell culture on gelatine coated plates - (reply: 1)
  195. easiest cell lines to use - (reply: 4)
  196. Jurkat cell - (reply: 4)
  197. Mesenchymal stem cells Expressing BDNF - (reply: 5)
  198. PI staining cell cycle analysis - (reply: 1)
  199. Protein Purification from mammalian cells - (reply: 1)
  200. How to identify CD8 T cell receptor for a particular antigen - (reply: 2)
  201. How to inactivate H1N1 virus in Cell culture supernatant ? - (reply: 3)
  202. HaCaT cell line - not responding to inflammatory stimuli such as LPS/TNF-alpha - (reply: 4)
  203. Homogenous Cell Lysates - (reply: 1)
  204. Does anyone irradiate their cells? - (reply: 3)
  205. need help for transfection to synchronized cells - (reply: 4)
  206. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
  207. What should I suspend my cells in prior to cytometry? - (reply: 2)
  208. Checking for efficieny competent cells - (reply: 1)
  209. Video on cell culture technique (broad brush) - (reply: 2)
  210. Setting up two drug kill curve for selection of stable cells - (reply: 1)
  211. No expression in stable transfected cell line - (reply: 2)
  212. Protocol for flow cytometry on mononuclear cells from mouse blood - (reply: 1)
  213. Cell line contamination? - (reply: 5)
  214. Inducing apoptosis by puromycin selection: toxic to the selected cells? - (reply: 1)
  215. Measuring cell proliferation from daily aliquots - (reply: 1)
  216. Cell culture of MYC immortalised HDFa - need advice - (reply: 1)
  217. Phoenix Amphoteric cell line to package lentivirus? - (reply: 6)
  218. miR30 based shRNA in mouse cells - (reply: 3)
  219. Cells are unhealthy - (reply: 2)
  220. Harvesting cells - (reply: 12)
  221. Dead cells after transfection - (reply: 9)
  222. preparation of cells for FACS analysis after transfection - (reply: 4)
  223. simultaneous knock down of two genes in a single cell line - (reply: 1)
  224. CoIP from cell lysate mix - (reply: 5)
  225. selective pressure on cells - (reply: 2)
  226. Conjugation of Ab to magnetic beads to isolate T cells from Spleen - (reply: 2)
  227. cell culture and biopsy - (reply: 1)
  228. Tiny black dots inside cell vacuoles - (reply: 1)
  229. I always have a cell depris floating in the supernatent of MDCK cells - (reply: 3)
  230. Is Trifluoroacetic acid toxic for cells? - (reply: 5)
  231. mycoplasma testing - (reply: 1)
  232. Treating suspension cells - (reply: 2)
  233. H9c2 cells..please help - (reply: 2)
  234. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
  235. How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (reply: 1)
  236. Establishing human neural stem cells - (reply: 3)
  237. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  238. Cell Adhesion and Motility on Fibronectin vs Collagen - (reply: 1)
  239. FCS in Serum free medium for better Cell Adhesion? - (reply: 1)
  240. Possible to sort cells from formalin? - (reply: 1)
  241. 293s vs HEK/293 cells - (reply: 2)
  242. Cell culture medium contamination? - (reply: 1)
  243. What do sick 293t cells look like? - (reply: 3)
  244. H2DCFDA and fixation of cells before flow cytometry analysis - (reply: 4)
  245. Decreasing cell division - (reply: 1)
  246. Can I use methanol fixation to fix cells before proteomic analysis? - (reply: 1)
  247. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  248. Do S2 cells normally have small 1-2um objects floating in culture? - (reply: 1)
  249. Problem in Cell Viewing - (reply: 3)
  250. How to view the encapsulated cells under microscope? - (reply: 5)
  251. Poor data quality using cultured cell in aCGH - (reply: 10)
  252. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  253. Totipotent Stem Cell From Molecular Components - (reply: 5)
  254. How to count clumpy epithelial cells? - (reply: 5)
  255. Why cells are floating after incubation in EBSS?? - (reply: 1)
  256. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  257. Live cell sort DNA stain permeabilization - (reply: 1)
  258. Problem in bacterial cell culture - (reply: 12)
  259. Persistent contamination in cell culture - (reply: 9)
  260. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  261. Adult stem cells - (reply: 2)
  262. Centrifuge stopped working while my blood samples were incubated in Cell lysis s - (reply: 1)
  263. Ways to Quickly Identify Antibiotic Resistance in Bacterial Cells (Non-tradition - (reply: 12)
  264. freezing competent cells without dry ice or liquid N - (reply: 3)
  265. Suspension cell line (RPMI) adhere to flask-WHY? - (reply: 1)
  266. Rapamycin Concentration for Cell Culture - (reply: 1)
  267. how to calculate cells and plate - (reply: 2)
  268. yeast conatamination in primary cells - (reply: 4)
  269. Maltodextrin will kill all the bacterial cells? - (reply: 5)
  270. Keratinocyte Culture: Cells detach - (reply: 7)
  271. Measure growth of plant cell culture - (reply: 1)
  272. Cell Lysate Western Blot Little or No Signal - (reply: 7)
  273. Cell line identity (HEK293-E) - (reply: 1)
  274. How do I calculate the enery absorved by cells form a UV light - (reply: 3)
  275. Immunocytochemistry - Washing away PC12 cells - (reply: 2)
  276. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
  277. Total Protein in COS-7 Cells - (reply: 1)
  278. protein extraction from cell nucleus - (reply: 2)
  279. Which gauge for proper shear force on certain cell dimension - (reply: 1)
  280. Mammalian Cell Lysis Buffer Triton-X100 Content - (reply: 6)
  281. DNA from frozed cells??? - (reply: 2)
  282. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
  283. Tumor cell analysis - (reply: 2)
  284. What can I do with my MCF7 cells? - (reply: 1)
  285. Evolution of Living Cells from Non-living Organic Matter - (reply: 3)
  286. cell lines - (reply: 5)
  287. M2 antibody background in Xenopus cell extracts - (reply: 6)
  288. Asking for guidelines to start L6 cell line culturing - (reply: 1)
  289. how to separate organelles from mammalian cells - (reply: 1)
  290. Cell culture contamination ? - (reply: 5)
  291. How to coat 96 well plate with poly-L-Lysine for cell culture use? - (reply: 2)
  292. Can serum free medium inactivate trypsin? - (reply: 1)
  293. Is Protein A present on the Staphylococcus saprophyticus cell wall too? - (reply: 2)
  294. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
  295. red dots in cell culture line? - (reply: 3)
  296. Integrity of cell monolayer - (reply: 1)
  297. problem with COV318 ovarian cancer cell line - (reply: 4)
  298. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  299. atom replacement in human cells - (reply: 2)
  300. What stage of growth are the cells I counted? - (reply: 4)
  301. HeLa cell culture - (reply: 6)
  302. Lambda Phosphatase Treatment of Cell Lysate - (reply: 1)
  303. Problem in Determination of Total Bacterial Cells - (reply: 13)
  304. Preparing a cell culture stock to plate out at 7x10^5 per well? - (reply: 1)
  305. Cell culturing tips - (reply: 2)
  306. SAOS.2 Cell detachment without using Trypsin. - (reply: 3)
  307. Normal cells or contamination?! - (reply: 4)
  308. H2DCFDA for MDCK cells - (reply: 2)
  309. immortalized human neuronal cells - (reply: 4)
  310. LNCaP Cell Clusters - (reply: 2)
  311. Freezing viable cells in 96-well format? - (reply: 3)
  312. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  313. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  314. Does fixation and permeabilization of cells affect their oxidative stress levels - (reply: 1)
  315. HBE cell culture CRL-2079 MEDIA question - (reply: 1)
  316. Cells dying in culture after 24 hours - (reply: 6)
  317. SH-SY5Y cells: basics advice - (reply: 1)
  318. Cell clumping: dead cells? - (reply: 6)
  319. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  320. growing cometent cells - (reply: 1)
  321. Where can I purchase HepG2.2.15 cell line - (reply: 1)
  322. Mycoplasma detection with ATCC kit - (reply: 1)
  323. RAW 264.7 cell line - (reply: 1)
  324. Mycoplasma infected cell line clean-up - (reply: 1)
  325. Rheumatoid Factor (RF) producing cell line? - (reply: 1)
  326. Weighing harvested cells - (reply: 1)
  327. Temperature Variation on Cell Growth Experiment - (reply: 1)
  328. Morphology of cultured cells - (reply: 5)
  329. Growing GH teratocarcinoma cell line- help needed - (reply: 2)
  330. Cells Detach After Transfection - (reply: 1)
  331. How to determine the location of integrated DNA in stable cell lines - (reply: 2)
  332. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  333. When cell media becomes "used up", it acidifies - (reply: 3)
  334. Cell cycle analysis for SubG1 fraction - (reply: 3)
  335. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  336. Cytokines feeding cell culture - (reply: 1)
  337. Experience with U-937 cell line - (reply: 3)
  338. Cell culture contamination issue - unable to identify the contamination - (reply: 1)
  339. cell confluency in IF - (reply: 2)
  340. Protein purification for adherent mammalian cells - (reply: 2)
  341. Antibody production in Expi293 cells - (reply: 2)
  342. death of transfected cells after first passage in G418 medium - (reply: 1)
  343. clonogenic assay for shRNA transfected cells - (reply: 3)
  344. Cell lysis in chromosome conformation capture - (reply: 2)
  345. Relationship between cell lines and cancer research (IHC) - (reply: 4)
  346. Serum starvation - something similar for serum-free kept cells ? - (reply: 2)
  347. Sodium bicarbonate addition to GMEM for BSR T7/5 cells - (reply: 2)
  348. No proteins after HeLa cell lysis - (reply: 3)
  349. How can i fix cultured cells on glass slides for insitu hybridization - (reply: 1)
  350. Blocking a protein in cell culture through antibody - (reply: 10)
  351. How to propagate Electro-competent DB3.1 cells - (reply: 6)
  352. Number of cell - (reply: 5)
  353. Difference between PFA, acetone and methanol in cell fixation - (reply: 1)
  354. cell proliferation statistics methods - (reply: 4)
  355. Losing cells during fixation - (reply: 2)
  356. ovarian cancer cell line: phenotype disappears - (reply: 3)
  357. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  358. HeLa Cells on Chin/in Nose? - (reply: 7)
  359. Can centrioles be seen when the cell is not undergoing cell division? - (reply: 3)
  360. How much ethanol (in micro g) has toxic effects on cells? - (reply: 1)
  361. penicillin/streptomycin in cell culture - (reply: 3)
  362. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
  363. Cell lysis for unknown reasons - (reply: 1)
  364. Preparing cells for plating - (reply: 3)
  365. ChIP with low cell number - (reply: 2)
  366. Mammalian cell culture and G418 selection - (reply: 1)
  367. Losing cells during intracellular staining method! - (reply: 1)
  368. G418 mammalian cell selection question - (reply: 3)
  369. DIFFERENCES BETWEEN GLIAL AND GLIOMA CELL? - (reply: 4)
  370. Bought HEK293T cells from ATCC -- passage number question - (reply: 1)
  371. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
  372. cell pellet question - (reply: 2)
  373. B cells cultures. Immunoglobuline production - (reply: 1)
  374. ChIP problem - using cells as a negative control - (reply: 7)
  375. Blood cell migration from mother to fetus - (reply: 1)
  376. Qiagen mini prep cell fragment pellet - (reply: 2)
  377. Cell death detection using adenosin kinase - (reply: 5)
  378. THP-1 differentiation using PMA yielding black dots within cell structure - affe - (reply: 4)
  379. Transfecting three plasmids into hES cells - (reply: 2)
  380. Agglomeration of suspension cells (T-cells) - (reply: 1)
  381. Selection of E.coli competent cells - (reply: 2)
  382. Chelating Aluminum from cell culture - (reply: 5)
  383. chemotherapeutic-resistant cell lines - (reply: 1)
  384. infection assay-buffer that lyses mammalian cells but not bacteria - (reply: 1)
  385. HEK293 vs. SH-SY5Y Cell lines? Which best for in vitro Alzheimer's Model? - (reply: 2)
  386. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
  387. Electroporate lentiviral vector for stable cell line - (reply: 2)
  388. Place to buy (soluble) ligands for cell culture. - (reply: 3)
  389. NIH 3T3 cells cross contamination - (reply: 1)
  390. NIH 3T3 cells - (reply: 3)
  391. Cells adhering to walls of conical tube? - (reply: 7)
  392. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  393. choosing the correct subculturing ratio - ovarian cancer cell lines - (reply: 1)
  394. RAW 264.7 cell line - (reply: 4)
  395. washing of attached bacterial cells - (reply: 3)
  396. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
  397. Cell Debris Isolation From Blood - (reply: 3)
  398. How to calculate drug amount to use in cell lines - (reply: 3)
  399. Growing certain number of bacterial cells in LB - (reply: 1)
  400. T cells or Lymphocytes? - (reply: 2)
  401. Western Blot with fixed cells - (reply: 5)
  402. Less than 10 GFP positive cells - (reply: 2)
  403. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
  404. How to culture HK-2 cells - (reply: 6)
  405. competent cells - (reply: 1)
  406. Debris in cell culture - (reply: 2)
  407. What housekeeper to use with HEK cells? - (reply: 3)
  408. Housekeeper for HEK cells? - (reply: 1)
  409. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
  410. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
  411. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
  412. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
  413. Gene expression profiling with small cell number - (reply: 1)
  414. Gene expression assay with small cell number - (reply: 3)
  415. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
  416. Stem Cell Therapy comes of age? Discussion Topic - (reply: 1)
  417. cell free DNA and maintaining stability - (reply: 3)
  418. Freezing Cells For Use In Assays - (reply: 2)
  419. INS-1E cells migration assay - (reply: 1)
  420. Short-term storage of HEK293 cells before FACS - (reply: 3)
  421. Sample storage for ELISA (cell culture supernatant) - (reply: 2)
  422. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
  423. Primary cell line - (reply: 5)
  424. 2D Image Primary Cell line - (reply: 13)
  425. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  426. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
  427. To small amount of cells used for shRNA transfection - (reply: 1)
  428. using cell line to generate standard curve - (reply: 6)
  429. Autofluorescence in thick structure cluster of cells - (reply: 4)
  430. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
  431. Serial dilution of cells - (reply: 3)
  432. Cloning repetitive sequences in mammalian cells - (reply: 3)
  433. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
  434. Plant Cells - what are they? - (reply: 1)
  435. HS-5 and HS27A cells - (reply: 1)
  436. MC-3T3 cells are not mineralizing... - (reply: 3)
  437. HS-5 cell culture "question" - (reply: 1)
  438. Problem with Kasumi-6 cell line - (reply: 1)
  439. G418(Geneticin) is not killing my cells - (reply: 2)
  440. Community feedback needed to clarify terms for a Cell Culture Ontology - (reply: 1)
  441. Quantify total DNA in small cell lysate...? - (reply: 1)
  442. Trouble validating in multiple cell types - (reply: 1)
  443. Gating of dendritic cells - (reply: 7)
  444. Please help me take a look at my cells to see whether is it contaminated - (reply: 3)
  445. cell binding assay with nanoparticles? - (reply: 2)
  446. Cell lines for clonal selection - (reply: 3)
  447. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
  448. SH-SY5Y Immortalized cell lines; researching neurotoxicity? - (reply: 3)
  449. Pelleting bacterial cells - (reply: 1)
  450. How viable is cell culture for a model? - (reply: 2)
  451. Help with adherent cell culture - (reply: 7)
  452. study of receptors in cell lines - (reply: 1)
  453. Cell line nomenclature - suffixes ? - (reply: 3)
  454. Maintainence of transformed cells - (reply: 5)
  455. improving viability of pelleted cells - (reply: 2)
  456. Buying Ins-1e cells - (reply: 1)
  457. How do/should cell biologists calculate power of analysis? - (reply: 1)
  458. Mycoplasma on normal hands? - (reply: 1)
  459. comparing ADCC effect in 3 cell lines. - (reply: 1)
  460. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
  461. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
  462. Trypan blue - (reply: 6)
  463. virus and cell surface receptor interaction - (reply: 4)
  464. Music Video on Cell Division/Mitosis - (reply: 3)
  465. How to select competent E. coli cells? - (reply: 7)
  466. Host cell protein sequence search - (reply: 1)
  467. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  468. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  469. Preparing supernatant from cell culture for western blot - (reply: 4)
  470. Fast/easy way to measure cell size - (reply: 4)
  471. Which is the best way to generate growth curves for cancer cell lines - (reply: 7)
  472. Cell number issue - Nile Red staining - (reply: 3)
  473. Music Video on Cell Division/Mitosis - (reply: 1)
  474. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
  475. Integrin B1 Staining in HT1080 cells - (reply: 1)
  476. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
  477. My cells won't pellet - any ideas? - (reply: 6)
  478. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
  479. EMSA: cell extract vs purified protein - (reply: 2)
  480. Insect cells transfection reagent - (reply: 1)
  481. Low Protein Content In Cell Lysate - (reply: 1)
  482. Can you see GFP after lysis mammalian cells? - (reply: 5)
  483. Centrifugation speeds for cells. - (reply: 8)
  484. Cell recovery from ethanol fixation - (reply: 4)
  485. Cryopreservation of MSC at lower cell density - (reply: 2)
  486. C-terminal GST fusion problem in mammalian cell - (reply: 6)
  487. Identify cell culture contamination - (reply: 2)
  488. Adipose stem cell culture - (reply: 5)
  489. Determine cell proliferation by viability assay ? - (reply: 6)
  490. Apoptosis via cell to cell contact - (reply: 1)
  491. Cells stop to grow - (reply: 6)
  492. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
  493. Long Treatment, Rapidly Dividing Cells - (reply: 7)
  494. Need help calculating cell number to plate - (reply: 1)
  495. Injection of human cell culture conditioned media into nude mice - (reply: 1)
  496. How long can I store cell lysis buffer at 4C for? - (reply: 1)
  497. Heeeeeelp..cell cycle sample..is it possible to store it? - (reply: 2)
  498. Subculture issue of primary cell - (reply: 6)
  499. Differentiating mouse from human cells with PCR - (reply: 5)
  500. primary valve interstitial cells transfection - (reply: 1)
  501. What to do about mycoplasma? - (reply: 9)
  502. Stem Cell Donation - (reply: 7)
  503. isolating DNA from cells in suspension - (reply: 5)
  504. Software for designing cell pathways - (reply: 1)
  505. Tamoxifen citrate solubility for cell work?? - (reply: 1)
  506. Centrifuged cells stick to wall of reaction tube - (reply: 12)
  507. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  508. Mycoplasma Test Kit - (reply: 1)
  509. Changing Genomic Regions in Host Cell - (reply: 1)
  510. TRYPSIN - (reply: 4)
  511. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
  512. my competent cell grow on any agar plate - (reply: 7)
  513. Bacterial Contamination in HuEmbryonic cell lines seen in SFM4 but not in DMEM + - (reply: 3)
  514. Cell attachement occuring after 48 hours, not 24 hours. - (reply: 3)
  515. Transfection of a non human gene into human cell line - (reply: 1)
  516. Procedures to replace vial of cells and passage number. - (reply: 4)
  517. Immunofluorescence on cells - can I keep the coverslips unstained in PFA? - (reply: 4)
  518. p14ARF and MDM2 in transformed cells - (reply: 1)
  519. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  520. contamination of human cell lines with yeast - (reply: 3)
  521. Generation of single cell suspensions from neural tissue - (reply: 2)
  522. Is this contamination or cell death? - (reply: 7)
  523. BaF3 cell culture in 96 well plates - (reply: 2)
  524. THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS) - (reply: 2)
  525. unwanted cell lysis during LB+Amp incubation - (reply: 5)
  526. Cells spontaneously detaching? - (reply: 1)
  527. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
  528. different type of media for cell culture - (reply: 1)
  529. Influenza: cell and mechanism information? - (reply: 1)
  530. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
  531. How to treat cells with TNFa - (reply: 2)
  532. CLOUDINESS of RIN5F cell culture - (reply: 7)
  533. U87MG cells not growing well - (reply: 3)
  534. Concentrating low volume of cells - (reply: 8)
  535. Preparing a conditioned medium from cancer cells - (reply: 3)
  536. Presence of melanin in melanin containing cell line - (reply: 1)
  537. Cancer cells culture - (reply: 1)
  538. How to achieve uniform cell distribution in culture vessle - (reply: 6)
  539. viability/integrity stain of fungal cells - (reply: 5)
  540. Cell culture contamination - (reply: 3)
  541. airway primary cells stop growing at passage 3 - (reply: 1)
  542. cell culture neural cells easier? - (reply: 6)
  543. Question - Corticosterone, cell culture - (reply: 1)
  544. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  545. Where to obtain rat embryonic stem cells? - (reply: 3)
  546. Stable cell line - (reply: 1)
  547. PBMC cells handling - (reply: 1)
  548. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  549. Unable to clone in E. coli cells - (reply: 3)
  550. removing MHC class I from the cell surface - (reply: 3)
  551. Osteosarcoma cell culture problem - (reply: 7)
  552. rtPCR stem cell signalling array from quiagen- has anybody experience? - (reply: 1)
  553. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 4)
  554. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 1)
  555. colon cancer cell lines vs normal colon epithelial cells - (reply: 1)
  556. Cell count and seed, plz help... - (reply: 4)
  557. In and out of cell Help 2 - (reply: 6)
  558. In and out of cell Help - (reply: 2)
  559. Primary culture of breast tumor cells - (reply: 1)
  560. Strange Cell Morphology & Cell Debris Following Lentiviral Transduction - (reply: 2)
  561. Cell numbers versus confluency - (reply: 2)
  562. Flow cytometry in THP-1 cells - (reply: 1)
  563. Competent bacterial cells - (reply: 2)
  564. Could a change of FBS affect outcome of a cell proliferation assay? - (reply: 4)
  565. How do they make passage limited cells? - (reply: 1)
  566. transient transfection problem using ME180 cells - (reply: 1)
  567. How to completely remove cells from surface - (reply: 3)
  568. Hiperfect siRNA in HT-29 cells help - (reply: 2)
  569. Isolation of BOTH cells AND serums from mouse blood - (reply: 5)
  570. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  571. Commercial source Stomach Smooth Muscle Cell lysate - (reply: 1)
  572. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
  573. cell line contaminated by something... - (reply: 2)
  574. Flag Staining in U87 cells - (reply: 4)
  575. protein is not expessing in HEK 293 cells - (reply: 2)
  576. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
  577. HepG3 cell line origin - (reply: 3)
  578. dilution of cell suspension - (reply: 4)
  579. how to preserve spleen cells for long duration, for flowcytometry - (reply: 2)
  580. Increasing cell growth rate in plain M9 medium - (reply: 1)
  581. Puromycin - shRNA - stable cell line for knock down - (reply: 4)
  582. Reducing cell adhesion - (reply: 1)
  583. The Hek 293T cell line looks like non adherent cells after to be sorted in FACS - (reply: 1)
  584. Hygromycin selection of virally-transduced cell lines - (reply: 1)
  585. Which cells express which genes? - (reply: 1)
  586. IGF1 stimulation of THP1 cells - (reply: 2)
  587. Cells detach during bodipy 493/503 staining - (reply: 1)
  588. RNA isolation from small numbers of cells - (reply: 6)
  589. Distinguish murine and human cells by surface staining? - (reply: 1)
  590. starting HaCaT cell line. basic questions - (reply: 6)
  591. culture media for pancreatic cancer stem cells - (reply: 2)
  592. Lysis method for single human embryonic cell - (reply: 5)
  593. In vitro transcription/translated protein to cells!!! - (reply: 1)
  594. Cell plating - (reply: 2)
  595. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
  596. colony formation assay in A2780 cells - (reply: 4)
  597. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  598. Which is a better way to culture cells after thawing ? - (reply: 2)
  599. Comet assay sperm cells - (reply: 5)
  600. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  601. Live/Dead Staining of already Permeable Cells - (reply: 10)
  602. cell synchronisation for fibroblasts (help choosing method/chemical) - (reply: 2)
  603. Cell Therapies - (reply: 2)
  604. What is the activity of 1X Trypsin solutions? - (reply: 3)
  605. SURE E. coli competent cells - (reply: 1)
  606. Stable protein expression in Vero cells - (reply: 2)
  607. HL60 cell line suppliers - (reply: 1)
  608. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
  609. Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
  610. Question about HeLa cell overconfluence - (reply: 3)
  611. 293T cell contamination post-transfection - (reply: 1)
  612. HEK293T cells are too fragile?? - (reply: 2)
  613. number of splitting after defrozing cells - (reply: 2)
  614. my human iPS cells have a lot of differentiation - (reply: 4)
  615. Properly designing a drug treatment assay measuring cell cycle arrest - (reply: 2)
  616. Detaching Breast Cancer cells (MCF-10A, HCC70) without trypsin- used for FACs - (reply: 4)
  617. counting cells before adding them to 96 well plate - (reply: 7)
  618. Cell Suspension - (reply: 2)
  619. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 5)
  620. MDA-MB-231 cells undergoing senescence- help! - (reply: 2)
  621. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  622. coating cover-slides before seeding cells for fluorescent microscopy - (reply: 4)
  623. How to induce early apoptosis in cells without using chemicals or UV - (reply: 5)
  624. choice of cell line for orthotopic xenograft implant - (reply: 3)
  625. Compound concentration depending on cell number? - (reply: 4)
  626. How to remove mycoplasma contamination in cell lines? - (reply: 5)
  627. [Question] How to remove cell debris stuck on the attached cells in primary card - (reply: 2)
  628. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  629. Reagent used to adjust pH of cell culture media - (reply: 3)
  630. Powder indomethacin in cell culture media?? - (reply: 2)
  631. How many generation of HEK293 cell can undergo? - (reply: 1)
  632. [Question] how to make primary-cultured cells evenly distributed? - (reply: 11)
  633. small dots in cell culture - (reply: 2)
  634. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  635. Clumpy competent cells - (reply: 3)
  636. Preparation of bacterial cells for SEM and TEM - (reply: 2)
  637. G418 advice in estab;lishing stable cell line - (reply: 1)
  638. Electroporation of J774.2 murine macrophage cell line - (reply: 1)
  639. About trypsinizing adherent cells in 96-well plates - (reply: 2)
  640. Passaging cells - (reply: 12)
  641. Preparing homemade XL1-blue cells in CCMB80 buffer - (reply: 1)
  642. Understanding Cell Passaging - (reply: 5)
  643. How to extract DNA from small number of cells - (reply: 2)
  644. How to identify beta pancreatic cells in dish of heterogenous cells? - (reply: 12)
  645. Cell Splitting Ratio Question - (reply: 5)
  646. Sf9 cell suspension culture - (reply: 5)
  647. competent cell formation - (reply: 1)
  648. Help! Clontech Xfect is killing my T-Rex HeLa cells!!! Why! - (reply: 1)
  649. How long are cells viable on ice - (reply: 2)
  650. Discrepancy between tissue and cell culture - (reply: 1)
  651. What is the density of a human skin cell? - (reply: 8)
  652. Determining the % of GFP/CHERRY cells in co-mixed culture - (reply: 3)
  653. Is the genomic DNA similar among different cell lines? - (reply: 4)
  654. serumm free culture of adherent cho cells - (reply: 1)
  655. detection of mesenchymal stem cells in cancer tissue sample - (reply: 1)
  656. Can u suggest any myoepithelial cell isolation protocol with the help of reagent - (reply: 1)
  657. cell line cultured in suspension - (reply: 1)
  658. GFP cells in paraffin.....orange? - (reply: 1)
  659. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
  660. Cell staining for fluorometric assay - (reply: 5)
  661. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  662. Long-Term Culturing of SH-SY5Y Cells - (reply: 1)
  663. N acetyl cysteine solution for cell culture - (reply: 3)
  664. Aortic smooth muscle cell contamination - (reply: 1)
  665. need recommendation for stable transduction of fibroblast cells - (reply: 1)
  666. Sub G1 in cell cycle - (reply: 2)
  667. DMSO induces cluster growing in LNCaP cells? - (reply: 1)
  668. Cell cycle arrest - adherent cells going into suspension - (reply: 4)
  669. Prevent adherent cells from adhering - (reply: 3)
  670. INS-1E cells not adhere to the flask! - (reply: 1)
  671. A549 cells are not attaching to flask - (reply: 2)
  672. irradiated J774 cells - (reply: 1)
  673. Cell lysis with proteinase k - (reply: 2)
  674. One siRNA sequence, many cell lines - (reply: 1)
  675. Freezing cell lysates for westerns - (reply: 2)
  676. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
  677. adherence to host cell question - (reply: 2)
  678. New patch of sf9 cells not growing - (reply: 2)
  679. contamination in yeast cell culture - (reply: 8)
  680. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
  681. sf9 Insect cells questions - (reply: 3)
  682. Kit to mesure cell death - (reply: 4)
  683. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  684. J774 and Flow cytometry....are they really big cells ? - (reply: 9)
  685. immunostaining cells grown in matrigel - (reply: 1)
  686. Cell concentration for flow cytometry - (reply: 2)
  687. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
  688. Cells growing very slowly - (reply: 6)
  689. SH-SY5Y cells do not grow - (reply: 1)
  690. GH3 cells - (reply: 4)
  691. The connection between LPS and PMA stimulation for maturation of ThP1 cells - (reply: 2)
  692. Using normal competent cells in electroporation method? - (reply: 1)
  693. Washing n2a cells out of 75 cm flask? - (reply: 1)
  694. How to dissociate N2A cells after centrifugation? - (reply: 3)
  695. Undefinable particles in cell culture flask - (reply: 2)
  696. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  697. immunostaining of cells in culture - (reply: 1)
  698. healthy stem cells vs cancer stem cells - (reply: 1)
  699. values for mRNA molecules per cell - (reply: 1)
  700. Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
  701. MCF10A Tet-ON stable cell line generation - (reply: 1)
  702. Primary cell line - (reply: 2)
  703. Neurosphere Assay with neuroblastoma cell lines - (reply: 1)
  704. How to decontaminate adherent cells with gentamicin - (reply: 2)
  705. Contamination in CHO cell ? - (reply: 3)
  706. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
  707. How to purify RNA from a handful number of cells? - (reply: 5)
  708. Check cell viablity after FACS - (reply: 2)
  709. ISH on fluorescing cells - (reply: 1)
  710. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
  711. Protein extraction from dead cells - (reply: 1)
  712. Cells in suspension vs. plated - (reply: 2)
  713. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  714. Hepg2 cells growing on top of each other - (reply: 1)
  715. urgent! calculating concentration of compound with different cell amounts - (reply: 3)
  716. h9c2 cells culture - (reply: 5)
  717. Does anyone have experience of subjecting cultured cells to mechanical stress? - (reply: 1)
  718. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
  719. cell population doubling - (reply: 1)
  720. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
  721. Cell line licence - (reply: 1)
  722. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
  723. temperature tolerance of animal cells - (reply: 3)
  724. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  725. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
  726. Human insulin for cell culture? - (reply: 2)
  727. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  728. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  729. Trouble producing stable cell lines - (reply: 2)
  730. Infecting cells with virus - (reply: 1)
  731. Requesting basic protocols on cell sorting - (reply: 1)
  732. lysing mammalian cells in sds - (reply: 3)
  733. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
  734. Is it possible to assay the carbohydrate profile of a cell? - (reply: 2)
  735. changing cell culture media - (reply: 1)
  736. Preparing Palmitate:BSA solution for treatment of cells? - (reply: 7)
  737. trypsinization of adherent cells - (reply: 9)
  738. Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
  739. Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
  740. How to lysis cells for an RNA extraction - (reply: 1)
  741. Problem with THP-1 cell line - (reply: 3)
  742. normalization to protein content or cell number - (reply: 2)
  743. detection of phosphorylation from cell pellet - (reply: 2)
  744. are THP-1 Cells(Monocytes) or Macrophages resistant to Mycoplasma infection or - (reply: 4)
  745. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  746. Cell culture shared with yeast? - (reply: 2)
  747. E. coli cells refuse to grow! - (reply: 2)
  748. how to fraction stem cell at different cell cycle stage - (reply: 2)
  749. Making Nuclei From Mouse Cells - (reply: 1)
  750. Different cell lines different protein stability - (reply: 1)
  751. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
  752. Immunofluorescence Staining of Cells - (reply: 1)
  753. Nucleus fills the whole cell - (reply: 3)
  754. Generating stable cell line with linear DNA - (reply: 3)
  755. isolating cells from Cockroach - (reply: 2)
  756. Question about suspension cells - (reply: 7)
  757. Calu-3 cell line do not grow properly - (reply: 1)
  758. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
  759. Counting and plating cells - (reply: 3)
  760. cells die 4-6 days after thawing - (reply: 8)
  761. what is the trypsin X for subculturing - (reply: 1)
  762. Cell Envelope Proteins Smeared - (reply: 1)
  763. Calculating the amount of the desired cells in the medium ? - (reply: 3)
  764. long term storage of cells at -80 - (reply: 1)
  765. Trypan Blue Exclusion Test - (reply: 2)
  766. Making BL21 competent cells - (reply: 2)
  767. cell plating density - (reply: 2)
  768. What kind of cell culture contamination is it? - (reply: 8)
  769. Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
  770. xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
  771. to treat cells with inhibitor - (reply: 2)
  772. lysis buffer not lysing adherent cells? - (reply: 8)
  773. Trypan Blue Staining - (reply: 3)
  774. Culturing cells on a slide - (reply: 5)
  775. Culturing cells on a slide - (reply: 3)
  776. protocol for immunostaining primery cells - (reply: 5)
  777. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
  778. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
  779. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
  780. Could the use of different FBS affact my cells - (reply: 5)
  781. Analysis of high throughput screen for cell surface markers - (reply: 1)
  782. Lymphoblastoid cell culture - (reply: 9)
  783. SF9 Cells lose exponential growth, lack stationary phase - (reply: 3)
  784. Don't know why my cells are unhealthy - (reply: 2)
  785. Hybridoma cells not secreting antibody - (reply: 3)
  786. Determining gene of interest from Cell culture lines - (reply: 2)
  787. Three clones of a cell line? - (reply: 3)
  788. Freezing cells in DMSO straight into minus 80? - (reply: 4)
  789. MCF7 cells vitality - (reply: 3)
  790. Ultrasonic bath for cell lysis - (reply: 3)
  791. any methods to evaluate the attaching ability of suspension cells? - (reply: 6)
  792. Vascular Smooth Muscle cell Marker - (reply: 3)
  793. Foam in Cell Culture - (reply: 1)
  794. Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
  795. SURE cells - (reply: 1)
  796. DT40 Cell Synchronisation - (reply: 2)
  797. Bio-Rad Cell counter - (reply: 3)
  798. SKBR-3 cells dying - (reply: 3)
  799. New cell culture user with no teacher or trainer - (reply: 5)
  800. Help with some problems with Culturing Epithelial Cells - (reply: 5)
  801. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
  802. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
  803. What are these large multinucleated cells??? - (reply: 1)
  804. Searching for a live cell staining method - (reply: 1)
  805. WT BRAF/NRAS mouse Melanoma cells - (reply: 2)
  806. HEK 293 cells Detachement - (reply: 8)
  807. Cell culture in candle jar - (reply: 2)
  808. centrifugation speed/cell prep for flow - (reply: 4)
  809. Huge background with HT22 cells phophoERK antibody - (reply: 3)
  810. Huge background with HT22 cells phophoERK antibody - (reply: 1)
  811. Competency cells analysis - (reply: 1)
  812. How many cells needed for co-IP and mass spec - (reply: 4)
  813. Performing MTT assay in suspension cells (new in the subject) - (reply: 6)
  814. cells die after transfection - (reply: 1)
  815. Seeding cells on small metal screws - (reply: 1)
  816. Cell lysate turbidity upon long storage - (reply: 3)
  817. Host cells for transformation - (reply: 3)
  818. Splitting or feeding cells for the weekend - (reply: 1)
  819. Black moving particles in the cell Culture - (reply: 2)
  820. lymphocyte proliferation using trypan blue - (reply: 2)
  821. cell culture - (reply: 1)
  822. cells shrink, detach from each other when changing media - (reply: 3)
  823. Newbie help: Seeding cells to 6-well plates - (reply: 2)
  824. 3t3 cell tured black - (reply: 9)
  825. calculating the number of cells to freeze down - (reply: 2)
  826. Fixed cells, sticky cells? - (reply: 2)
  827. What is cotransfection in molecular cell biology? - (reply: 1)
  828. BSA in adipocyte cellculture? Alternatives?? - (reply: 1)
  829. serum deprivation of cell lines - (reply: 1)
  830. serum deprivation of cell lines - (reply: 1)
  831. What to do Post-transfection of cells with siRNA? - (reply: 2)
  832. Problem with HEK stable cell line - (reply: 1)
  833. HEK 293 cell culture - (reply: 3)
  834. HepG2 cells resistant to Trypsinization - Please help!! - (reply: 8)
  835. difficulty in passaging HK-2 cells - (reply: 5)
  836. difficulty in passaging HK-2 cells - (reply: 2)
  837. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  838. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
  839. More cells in an antibiotic plate than in a plate without antibiotic???? - (reply: 2)
  840. what are these cells?? - (reply: 1)
  841. How to choose the right plasmids for certain types of cell? - (reply: 1)
  842. Isolation of rat endothelial cells - (reply: 2)
  843. protocol for making e.coli competent cells under low temperature - (reply: 1)
  844. Looking for cell surface markers. - (reply: 3)
  845. common nuclear marker for T cells - (reply: 1)
  846. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
  847. Any reliable markers for mouse memoery T cells? - (reply: 1)
  848. Do mouse endothelial cells express the alphaVbeta3 integrin? - (reply: 1)
  849. Cell Culture debris or contamination? - (reply: 4)
  850. primary cell isolation from CNS, how to remove myeline debris - (reply: 1)
  851. Platinum-A cell trasnfection toruble - (reply: 1)
  852. cell culture media refrigeration - (reply: 4)
  853. abnormal epithelial cells in mouse mammary gland - (reply: 1)
  854. cell DNA content of cells grown on different scaffolds - (reply: 2)
  855. Cancer cell starvation - (reply: 1)
  856. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
  857. Housekeeping gene for COS-1 cells - (reply: 1)
  858. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
  859. Monocytes die after cell scraping - (reply: 2)
  860. The water pan got dry in the TC incubator. Will my cells be OK? - (reply: 1)
  861. Cell fractionation - (reply: 2)
  862. Media formulations for MDA-MB-231, BT-474 cells and Caco-2 cells - (reply: 1)
  863. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
  864. Dropped cells into liquid nitrogen tank - (reply: 3)
  865. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
  866. Small intestinal cell line for transport studies - (reply: 4)
  867. help needed in determining volume of cells to be seeded - (reply: 6)
  868. Dilution, Cell concentration & DNA content - (reply: 3)
  869. competent cells - (reply: 2)
  870. Cell lines & gene of interest - (reply: 1)
  871. Software for selection genes related to concrete cell processes - (reply: 1)
  872. Immunocytochemistry with HEK293 cells - (reply: 1)
  873. How to determine apoptotic cell types in-vivo? - (reply: 1)
  874. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
  875. data analysis for single cell size and distribution - (reply: 1)
  876. determining optimal cell count for MTT - (reply: 2)
  877. Extracting DNA from FACS sorted cells - (reply: 3)
  878. Storing frozen cells in -80C instead of liquid nitrogen - (reply: 7)
  879. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
  880. DG44 CHO cells and Methotrexate - (reply: 1)
  881. extract plasmids from transfected eukaryotic cells - (reply: 6)
  882. Trypsin Deactivation - (reply: 4)
  883. Staining Cells in Suspension - (reply: 4)
  884. cell culture confusion - (reply: 1)
  885. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  886. chambered cell culture-slide for confocal microscopy - (reply: 3)
  887. Nicotiana tabacum (BY-2) Suspension cells contaminated! Help! - (reply: 1)
  888. urgh...mycoplasma drama..... - (reply: 5)
  889. Dead or Alive... -_- (MCF-7 cells) - (reply: 7)
  890. how to wash cells after fixation for a live binding study - (reply: 1)
  891. Evenly distribute 100ul of cells in a 48/96 well - (reply: 2)
  892. Cell lysis before shearing - (reply: 5)
  893. really low puromycin concentration for selection of cells - (reply: 6)
  894. Single stain control for 293 cell line - (reply: 4)
  895. Is mCherry denatured by Trypsin or RIPA buffer? - (reply: 7)
  896. loading dendritic cell with antigen - (reply: 1)
  897. Cell Lysis and Western Blotting - (reply: 1)
  898. problem with embryo carcinoma cell line , p19 , - (reply: 1)
  899. In vitro induction of apoptosis in tumor cells by inactivated NDV and IAV - (reply: 1)
  900. problem with embryo carcinoma cell line , p19 , - (reply: 1)
  901. Recombinant protein from cell lysate - (reply: 4)
  902. How to test if a compound is entering cells? - (reply: 3)
  903. help with cell seeding - (reply: 2)
  904. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  905. RNA isolation from frozen cells - (reply: 2)
  906. How do you coat coverslips before seeding cells? - (reply: 3)
  907. Buy.Ask for buying some cell lines and antibodies - (reply: 3)
  908. competentce cell, cloning - (reply: 2)
  909. Flp-recombinase target site integration in 293 T-REx cells - (reply: 1)
  910. Storage of transfected PC12 cells - (reply: 1)
  911. cell adhension - (reply: 1)
  912. Using Methanol For Cell Lysis - (reply: 1)
  913. Adjusting pH of cell culture medium for ELISA - (reply: 1)
  914. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
  915. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
  916. Contamination of primary cells with fibroblast. - (reply: 1)
  917. Help With Lysing HEK293 & Fibroblast Cells - (reply: 1)
  918. problem with siRNA transfection of HEK cells - (reply: 5)
  919. Splitting A549 cells - (reply: 9)
  920. NMDA Transfection in 293 Cells - (reply: 1)
  921. freezing adherant cells - (reply: 2)
  922. freezing adherant cells - (reply: 2)
  923. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
  924. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
  925. cells stably expressing T7 RNA polymerase - (reply: 1)
  926. Cell Fixation - Experience with cell fixation? (reply: 5)
  927. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  928. Is pCL-Eco suitable for packaging viruses that used to infect human cells? - (reply: 1)
  929. Cause of Death for Cell Culture? - (reply: 5)
  930. creat a SY5Y stable cell line - (reply: 1)
  931. selecting drug-resistant colon cancer cells - (reply: 2)
  932. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
  933. Helper T cell skewing - (reply: 3)
  934. Optimum serum starvation period for NIH, CHO, COS7 cells - (reply: 4)
  935. Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
  936. competent cells stock - (reply: 3)
  937. intracellular cytokine staining - re-stimulation of activated T cells (reply: 1)
  938. DH5alpha cells - (reply: 4)
  939. pheonix cells and &293A cells - (reply: 1)
  940. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
  941. Protein purification from HEK 293 cells - (reply: 1)
  942. Growing hybridoma cells in medium without serum - (reply: 2)
  943. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
  944. Cell drawing tools? - (reply: 6)
  945. How many cells in plant embryos ? - seed biology (reply: 1)
  946. cell count - (reply: 8)
  947. question on making chemical and electrocompetent cells - (reply: 2)
  948. COS7 cells giving me problems - (reply: 1)
  949. Where to get 5T series cells? - (reply: 1)
  950. cell number for colony formation - HEK293, NIH3T3 (reply: 1)
  951. Electroporation/electrotransfection mammalian cells - (reply: 7)
  952. Cell debris here, there, everywhere!? - (reply: 2)
  953. Cell debris here, there, everywhere!? - (reply: 1)
  954. Mini prep of frozen bacteria cells - (reply: 2)
  955. Help! Turned on UV light. Did I kill my cells? - (reply: 2)
  956. growing competent cells - (reply: 1)
  957. Freezing density for NIH 3T3 cells - (reply: 3)
  958. Stable Cell Line generation - (reply: 3)
  959. making more competent cells - (reply: 2)
  960. CellTracker dye in necrotic cells? - (reply: 1)
  961. The case of the punky Sf9 cells - (reply: 2)
  962. sticky perm cells (tween 20)- can I prevent it? - (reply: 2)
  963. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
  964. collecting cells HT1080 - (reply: 4)
  965. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
  966. Help: problem with MDCK cells - (reply: 2)
  967. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
  968. Oh my cell culture problems never cease!!! - (reply: 4)
  969. WEHI-3BD cell culturing - Advice on culturing. (reply: 5)
  970. cell homogenization - (reply: 13)
  971. cell count - (reply: 3)
  972. cell lysis - confocal slides (reply: 4)
  973. HeLa cell anatomy question - (reply: 3)
  974. Need cell lines wich express separately TLR 2, 4 and 5 - (reply: 1)
  975. MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
  976. THP-1 cell line - (reply: 3)
  977. CaMKII alpha promoter and cell tranfection - (reply: 1)
  978. Adaptation of mammalian cells to serum free mode - (reply: 2)
  979. Live cell Imaging medium - (reply: 1)
  980. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  981. MDA-MB-231 cell transfection - (reply: 5)
  982. Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
  983. Break Cell Clumps - prepare single cells for injections (reply: 1)
  984. Stem Cells Sphere - stem cells (reply: 2)
  985. Request for cell lines? - (reply: 1)
  986. Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
  987. MTT problems with Huh7 cell line, plzzzzzzz help - MTT color problem (reply: 5)
  988. Calculating cell doubling time - (reply: 1)
  989. SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
  990. Infected cells that are dying after puromycin selection - (reply: 1)
  991. contamination issue in cell culture - (reply: 1)
  992. Why do cells need to be washed in PBS before biotinylation? - (reply: 3)
  993. AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD (reply: 2)
  994. Purification of recombinant proteins from insect cells - (reply: 2)
  995. Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
  996. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
  997. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
  998. Growth curve for PC3 cell line - (reply: 1)
  999. Agrobacterium EHA101 competent cells - (reply: 1)
  1000. THP-1 cell line - (reply: 2)
  1001. Effective DCFDA Protocol for Non-Adherent THP-1 Cells? - (reply: 1)
  1002. Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
  1003. best freezing method for electrocompetent cells - (reply: 9)
  1004. cell seeding calculation - (reply: 8)
  1005. Improved Stem Cell Proliferation - Would interested in improving proliferation of adult stem cells? (reply: 5)
  1006. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  1007. Yeast contamination in cell culture - (reply: 8)
  1008. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
  1009. Remove cells from dish and fix them in slides - (reply: 3)
  1010. Help- lysing cells with triton x-100 - (reply: 1)
  1011. E.coli cells lyse in minimal media - (reply: 3)
  1012. How stable are suspension cells in room temperature? - (reply: 2)
  1013. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
  1014. Transfection in insect cell lines?? - (reply: 1)
  1015. PCR genomic DNA using cell lysate - (reply: 1)
  1016. HELA Cells Capable of producing whole, infective HPV particles? - (reply: 3)
  1017. Distilled water for Electrocompetent Cell Preparation - (reply: 6)
  1018. Should I fix cells digested from tissues? - (reply: 4)
  1019. STR cell line identification - looking for cell line identification method (reply: 1)
  1020. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
  1021. Rat Dental Pulp Stem Cell Culture - (reply: 1)
  1022. NK cell analysis using CD16PE and CD56PE - (reply: 2)
  1023. My neuroblastoma are floating!! please help - Both SK-NSH and SH-SY5Y are adherent cells, but are only floating now (reply: 3)
  1024. cell suspencion and medium - (reply: 5)
  1025. One Shot TOP10 Cells - (reply: 6)
  1026. trypan blue for chlorella - (reply: 1)
  1027. Help on BmN4 cell culture - How to maintain BmN4 cells? (reply: 1)
  1028. Compare ChIP to cell lysate - (reply: 1)
  1029. Compare ChIP to cell lysate - (reply: 1)
  1030. Testing cytoxixity on healthy human cells - (reply: 2)
  1031. what cell line to use - to demonstrate ubiquitination (reply: 1)
  1032. GFP cells intracellular staining - (reply: 1)
  1033. Xenograph - how to maintain cell viability? - (reply: 1)
  1034. Western blot of apoptotic cells - (reply: 2)
  1035. cell sycle and proliferation - (reply: 3)
  1036. help with cell dilution - (reply: 2)
  1037. Difficult Cell Proliferation Experiment - (reply: 2)
  1038. Cell dilution cloning - Cells cloning (reply: 2)
  1039. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
  1040. HTC116 cells detaching !!!! - (reply: 2)
  1041. Revival of kasumi-1 cells - (reply: 3)
  1042. HEK 293 Trypsin vs. only EDTA - (reply: 2)
  1043. Growth conditions for E. coli DB3.1 cells - (reply: 1)
  1044. Mycoplasma cleanup time... - (reply: 10)
  1045. weird & curious problem with CHO cell culture - (reply: 3)
  1046. HaCat do not grow after using cell scraper - (reply: 5)
  1047. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
  1048. Cell Lysates from a Western Blog - (reply: 2)
  1049. Loss of YFP signal in transduced T cells - (reply: 7)
  1050. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
  1051. Freezing cell pellets and minimizing lysis - (reply: 2)
  1052. Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells? (reply: 6)
  1053. Total Cell protein - (reply: 1)
  1054. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
  1055. Mycoplasma - (reply: 1)
  1056. cell culture media - glutamine! - (reply: 2)
  1057. How many cells do you have in a confluent T 75 flask? - I have 1/2 million (reply: 2)
  1058. how to make antigen by trypsin and papain digetion - plz teel me hoe to make antigen by trypsin & papain digetion (reply: 1)
  1059. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
  1060. Premature cell lysis - (reply: 2)
  1061. urgent!!! weird Growth in cell line - (reply: 8)
  1062. Shipping of blood cells over long distance - (reply: 1)
  1063. refrozen cell pellet - (reply: 1)
  1064. Drug vehicle giving toxicity in cell culture - With increse in vehicle..... toxicity decrese (reply: 4)
  1065. contamination in cell line...... - (reply: 4)
  1066. Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 7)
  1067. Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
  1068. MTT with cells growhing in suspension, not adherent - (reply: 3)
  1069. Cell ELISA - No differences between cells expressing and not, the receptor (reply: 2)
  1070. growth curve for attached cells - (reply: 1)
  1071. cells dont attach hard enough to 96 well - (reply: 3)
  1072. CTLL-2 culture - preventing cell clumping - (reply: 2)
  1073. Yet another contamination question - SF9 cells (reply: 11)
  1074. Problems with transfection in human cells - (reply: 3)
  1075. HepG2 cells culture conditions - (reply: 2)
  1076. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
  1077. T cell proliferation problem - (reply: 3)
  1078. How to Handle Cells Shipped Internationally in Dry Ice? - (reply: 2)
  1079. Differences between cells in different species - (reply: 5)
  1080. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
  1081. isotype control for GFP expressing cells? - (reply: 2)
  1082. Trypsin for J774A Macrophages? - (reply: 2)
  1083. Trouble with growth of transformed cells - (reply: 4)
  1084. nuclear extraction of blood cells - (reply: 2)
  1085. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  1086. ELISA with undiluted cell lysate samples - (reply: 1)
  1087. How can you expand cells if you have one dish of them? - (reply: 1)
  1088. Androgen independent LNCaP cells - (reply: 2)
  1089. mycoplasma detection kit - (reply: 1)
  1090. What is the proper way to infect cell culture with virus? - (reply: 5)
  1091. Micro-RNA profiling for differentiating cells - (reply: 1)
  1092. Growing insect cells with other cell lines - (reply: 2)
  1093. Candida albicans cells lysis - method (reply: 1)
  1094. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
  1095. Advise for Whole Cell Stain - hESC-differentiated Trophoblast (reply: 1)
  1096. 293 T cells - (reply: 3)
  1097. Mouse Bone Marrow Isolation - How to increase yield of BM-derived cells? (reply: 1)
  1098. BL-21 Gold (DE3) cells and further strains - (reply: 2)
  1099. Cell Cycle Analysis flow cytometry - How to measure desynchronisation rate of cells (reply: 1)
  1100. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
  1101. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
  1102. Fixing cells before staining of surface markers - (reply: 1)
  1103. Black dots in cell culture - (reply: 7)
  1104. cell counting - (reply: 5)
  1105. Optimization for cell viability assay (MTT assay) - (reply: 1)
  1106. Anyone thought about reprogram fibroblast to liver cells? - (reply: 2)
  1107. cell confluence in cell cultures - myoblast c2c12 in particular (reply: 2)
  1108. cell line adherence - cells adhering to flask very tightly after transfectiono (reply: 1)
  1109. Cell labelling - How can I label 2 cell populations? (no transgenes) (reply: 5)
  1110. How long does it take for cells to reattach? - From balls to pancakes. (reply: 3)
  1111. Dot plot cell cycle analysis-strange dots - (reply: 4)
  1112. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
  1113. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  1114. help Splashed Raji cells on skin/face/hand - (reply: 2)
  1115. Help! ES Lenti infection efficiency=nightmare - trouble getting over 40 % cells infected (reply: 8)
  1116. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  1117. CO2 level for cell culture - (reply: 6)
  1118. sphere and suspension culture subculturing - (reply: 1)
  1119. suspension cells for confocal - (reply: 1)
  1120. RNAi is somatic and germline cells - (reply: 3)
  1121. Luciferase and cell responsiveness - (reply: 3)
  1122. adult cell culture - (reply: 1)
  1123. protein overexpression in HeLa cells - (reply: 1)
  1124. Insulin supplements to cell culture media - (reply: 1)
  1125. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
  1126. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
  1127. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
  1128. topic and cell culture - (reply: 2)
  1129. Running a western with cell media in gels? - (reply: 11)
  1130. Starting cells for making one's own competent cells - (reply: 7)
  1131. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  1132. Persistent stable cell line (Bos23) Contamination... - (reply: 2)
  1133. Hematopoietic cells separation - How to get high purity and viability hematopoietic cell populations? (reply: 2)
  1134. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  1135. min6 cells immunofourescence staining - staining and culture problem (reply: 4)
  1136. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
  1137. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  1138. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
  1139. rat ES cells - (reply: 2)
  1140. Mycoplasma contamination - (reply: 1)
  1141. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
  1142. establishment of primary cell line from biopsy - (reply: 2)
  1143. Cell culture fasting - (reply: 1)
  1144. mutated HeLa cells? - from cycloheximide? (reply: 1)
  1145. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
  1146. qPCR for transformed cells - (reply: 1)
  1147. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
  1148. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
  1149. descrepancy between total cell lysate and cell fractionation - (reply: 1)
  1150. Disruption of cells using mild detergent - (reply: 2)
  1151. Eruption of mammalian cells using detergent - (reply: 1)
  1152. lenti virus transfected cell dies... - (reply: 4)
  1153. Extremely Weird Issue - CHO Cell Culture (reply: 2)
  1154. Cell Die.. Need help - (reply: 1)
  1155. Cell scraping ruining my life - (reply: 6)
  1156. How much trypsin/Cause of death - (reply: 3)
  1157. Death of cells in the control. - (reply: 1)
  1158. cell culture clarification - I need help please (reply: 2)
  1159. Serum concentration in treatment of cells for MTT assay - (reply: 1)
  1160. Minimum number of cells needed for cytometry? - (reply: 3)
  1161. Beta-Lactam in CHO Cells - (reply: 1)
  1162. problems with 293T cells after medium removal!!! - (reply: 6)
  1163. B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
  1164. Propagating ccdB survival cells - (reply: 6)
  1165. Western difficulty in primary neuron cells. - (reply: 1)
  1166. DOubt - Competent cell (reply: 2)
  1167. Differentiation of CD11b cells into macrophages.Help please - (reply: 1)
  1168. Please Help!! - Mycoplasma (reply: 5)
  1169. Primary keratinocyte isolation- cell loss - (reply: 1)
  1170. cells attach but don't flatten out - MDA-231 (reply: 8)
  1171. Problem Generating Stable HeLa Cells - (reply: 1)
  1172. Retroviral transduction of T cells - (reply: 4)
  1173. Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
  1174. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
  1175. Multiple nuclei?? - cell morphology - FaDu (reply: 8)
  1176. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  1177. how to compensate the cell death during transfection by electroporation - (reply: 2)
  1178. H-ras transformed RK3E/tv-a cells - (reply: 1)
  1179. HEK001 Cell Line and Papillomavirus - Does HEK001 cell line produce a functional papillomavirus (reply: 4)
  1180. 2 questions: myeloma cell lines and mice MABs - (reply: 5)
  1181. I'm getting fried-egg looking cells... What should I do?? - (reply: 8)
  1182. Empty bubbles in the 3T3-L1 cells - (reply: 3)
  1183. Exposing Cell Lines to Drugs Metabolized by Liver Microsomes - (reply: 1)
  1184. UV safety in cell culture hood - (reply: 3)
  1185. Where can I find info on the physical appearance of TB cell wall structures? - I am an artist wanting to depict the cell wall with absolute accuracy. (reply: 7)
  1186. intracelluar staining without killing cells - (reply: 3)
  1187. Info about A7r5 cell culture - (reply: 1)
  1188. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  1189. Cell lysates - Protein precipitation? - (reply: 3)
  1190. Glucose in epithelial cell - (reply: 1)
  1191. cell lysate for pulldown - (reply: 3)
  1192. cell culture media - (reply: 3)
  1193. Competent cell storage for long term use - (reply: 2)
  1194. My RIPA cell lysis buffer - Western blot (reply: 3)
  1195. Cell Uptake Study - (reply: 1)
  1196. what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
  1197. aspects of RAW264.7 cells - (reply: 5)
  1198. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
  1199. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
  1200. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
  1201. Hoechst Stain does it stain dead cells - (reply: 3)
  1202. Mycoplasma detection - (reply: 2)
  1203. Help for cell preparation for FACS... - (reply: 1)
  1204. Cell Lysis Buffer - (reply: 6)
  1205. HeLa cells and HPV - (reply: 2)
  1206. Resolving cell growth problems using growth additives - way to resolve urgent cell growth problems (reply: 3)
  1207. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  1208. problem with MACS cell isolation - (reply: 5)
  1209. cell culture split ratios. - (reply: 2)
  1210. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
  1211. detection of mycoplasma with hoechst staining - (reply: 1)
  1212. blood red cells - (reply: 1)
  1213. Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
  1214. NFKb signalling in cell line ??? - (reply: 2)
  1215. kit fpr cell membrane preparation? - (reply: 2)
  1216. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
  1217. How to find NFKb signalling is active in a cell line??? - (reply: 2)
  1218. Fixing YFP cells - (reply: 2)
  1219. MTT Assay: cell proliferation or cell viability??? - (reply: 4)
  1220. Rescue RAW cell culture - RAW cells past confluent point... can they be rescued? (reply: 4)
  1221. cell cycle arrest by colcemid - (reply: 2)
  1222. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
  1223. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
  1224. PROLIFERATION ASSAY IN MICROTITER PLATES WITHOUT A SEMIAUTOMATIC CELL HARVESTER - (reply: 1)
  1225. THP cells? what does THP stands for?????????? - (reply: 1)
  1226. Immunioprecipitation from Jurkat cells?? - (reply: 1)
  1227. Weird effect of siRNA on protein in cells - (reply: 3)
  1228. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  1229. EDTA addition to trypsin solution (1x) - (reply: 1)
  1230. Apoptosis induction - for vero and mcf-7 cells (reply: 5)
  1231. Need pH 6 Cell culture media - (reply: 1)
  1232. Are my cells undergoing epigenetic changes? - (reply: 2)
  1233. establishment of stable cell line using hek293t - (reply: 2)
  1234. Cell pellet frozen -80 then -20 - (reply: 4)
  1235. total cell protein vs membrane protein - (reply: 4)
  1236. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  1237. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
  1238. Fixing the cells for FACS - Paraformaldehyde or Formaldehyde (reply: 2)
  1239. Problem with control samples in cell IC - (reply: 3)
  1240. Recommendation for cell cycle analysis software needed - (reply: 1)
  1241. Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
  1242. Cell culture contamination question - what the hell are these things?? - (reply: 3)
  1243. 'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
  1244. Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
  1245. Immunofluorescence using dead cells? - (reply: 1)
  1246. floating THP-1 cells - medium change advice - (reply: 2)
  1247. my cells love infection, any thoughts on antibiotics? - (reply: 3)
  1248. Culture Media for stem cells - (reply: 1)
  1249. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
  1250. Anyone heard of cells spontaneously fluorescing red?? - (reply: 4)
  1251. Freezing cells - How long can I keep cells at -80C? (reply: 6)
  1252. Help regarding cell line - (reply: 1)
  1253. organizing cell lines - (reply: 1)
  1254. Cell plating density - (reply: 3)
  1255. Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
  1256. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
  1257. MDA-MB-231 cells question - (reply: 3)
  1258. thawed cells wont adhere - pc3 and LNcaP (reply: 2)
  1259. DiFi Cell line (colon carcinome) - Where can I get it???? (reply: 1)
  1260. HaCaT cells - (reply: 2)
  1261. lncap and MCF-7become ethanol sensitive? - are lncap cells and MCF-7 cells ethanol-sensitive? (reply: 2)
  1262. Dying endometrial stromal cells - (reply: 1)
  1263. Help me - Are my cells infected with something or could this be unsusual morphol - (reply: 5)
  1264. how to dissociate cells for cell migration assay - (reply: 3)
  1265. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
  1266. RNA extraction - how many cells do I need at least?? (reply: 4)
  1267. RNA extraction protocol for small amounts of cells - (reply: 2)
  1268. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
  1269. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
  1270. strange morphology of sp2/0 cells after fusion!!! Please help! - morphology switch from round to triangular!!!! (reply: 2)
  1271. fabrablast primery cell line problem - how to detach cell well (reply: 1)
  1272. what solvent is better compatible for most cell lines? - DMSO vs methanol (reply: 3)
  1273. Cell growth rate & O2 consumption - (reply: 2)
  1274. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
  1275. Cells stick to dish in Embryoid Bodies - (reply: 1)
  1276. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  1277. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
  1278. how to plate cells precisely - (reply: 1)
  1279. RNA extraction from stained cells - (reply: 2)
  1280. Methods to assess cell viability - (reply: 1)
  1281. suspension cell transient transfection - (reply: 10)
  1282. Adherent cells washing off in 96 well -MTT - (reply: 4)
  1283. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  1284. plasmids - plasmids for stable cell line construction (reply: 2)
  1285. abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
  1286. average total protein content per 293t cell - (reply: 2)
  1287. T cells preparation for FACS - (reply: 1)
  1288. counting cells - (reply: 3)
  1289. Cell OD and spec problems - (reply: 1)
  1290. cell confluency & effect on cells...?? - (reply: 4)
  1291. Pipet trypsin tips - (reply: 3)
  1292. Fixing cells for flow cytometry - alternative to 1% paraformaldehyde (reply: 3)
  1293. mycoplasma false positive - Luminescence false positives for myco (reply: 2)
  1294. Washing Red Blood Cells - Tips on washing red blood cells (reply: 8)
  1295. MEF Cell marker - (reply: 1)
  1296. (CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
  1297. Change of colour in cell culture media...? - (reply: 2)
  1298. cell count - how to calculate (reply: 2)
  1299. generating cell line knockouts - (reply: 3)
  1300. Cancer Stem Cells?? - (reply: 10)
  1301. Some kind of contamination? - mouse transduced cell line (reply: 7)
  1302. Cell stimulation of transcription - (reply: 5)
  1303. Mouse blood density/osmolarity of blood cells for separation - (reply: 5)
  1304. suitable cell lysis buffer - (reply: 1)
  1305. Toluidine blue staining for cell culture - (reply: 1)
  1306. cell culture - 3D cell culture (reply: 1)
  1307. Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
  1308. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  1309. MEF culture contamination? - Contaminated cell culture? (reply: 2)
  1310. very simple question about hemocytometer cell counting - (reply: 1)
  1311. nucleic acid extraction from silenced cells - (reply: 6)
  1312. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
  1313. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
  1314. In Vitro Drug Exposure Cytotoxicity Testing and Cell Confluency - (reply: 8)
  1315. Dendritic cells - Protocol of stimulation of dendritic cells (reply: 3)
  1316. giant cells in HEK293 cell line - (reply: 10)
  1317. Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
  1318. Plant cell transfection - (reply: 1)
  1319. stable cell line - selection after retroviral infection (reply: 1)
  1320. Storing cell lines long-term at -70C - (reply: 4)
  1321. Commercially available primary mouse endothelial cell line? - (reply: 2)
  1322. Frozen cells in -80 - (reply: 1)
  1323. Plant or Fungal Cell Dissociation Technique? - (reply: 3)
  1324. how can i bring cells onto a slide? - ...for microscopical observation! (reply: 7)
  1325. cell density to be seeded? - cytotoxicity studies (reply: 1)
  1326. Lysis of the cells - (reply: 1)
  1327. Cells do not lyse in SDS buffer - (reply: 1)
  1328. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  1329. lysis buffer for lymphoblast cells - (reply: 1)
  1330. problem with cell cycle analysis - (reply: 3)
  1331. THP-1 Cells differentiation - THP-1 cells differentiated using IFN-gamma/LPS (reply: 7)
  1332. Single cell suspension from solid tumor - (reply: 3)
  1333. cell aggregation? - (reply: 1)
  1334. T-Cell receptor - Is it a single type on a T cell or multiple types? (reply: 1)
  1335. cells weak adhesion - detachment (reply: 4)
  1336. how we count the passages in cell cultures? - (reply: 5)
  1337. resolving cell growth problems - (reply: 2)
  1338. clumping of cells - (reply: 7)
  1339. stability of cell suspension - (reply: 1)
  1340. cell adhesion in serum free condition - (reply: 1)
  1341. effects of cell confluency - (reply: 1)
  1342. Which trypsin EDTA to use? - (reply: 3)
  1343. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
  1344. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  1345. polarized cells - (reply: 4)
  1346. PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
  1347. cell adhesion assay - using collagen (reply: 1)
  1348. Problem clariflying/filtering insect cell lysate - (reply: 2)
  1349. cell culture urgent!! - (reply: 2)
  1350. Moving S2 cells from one plate to another - (reply: 1)
  1351. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
  1352. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
  1353. mononuclear cells medium - I am stuck with the concentration of 2-mercaptoethanol (reply: 1)
  1354. Accidental thawing of frozen cells - (reply: 3)
  1355. how to start with a new cell line ? - how to start with a new cell line ? (reply: 1)
  1356. HaCaT cells die after trypsinization - (reply: 1)
  1357. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
  1358. GFP and Luciferase Cell Line - (reply: 2)
  1359. Transfecting primary cells with plasmid - (reply: 1)
  1360. transfecting and selecting EL4 cells - (reply: 1)
  1361. Induce Osteoblast lineage from C2C12 myoblast cells - (reply: 1)
  1362. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
  1363. Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
  1364. Transfection into eukaryotic cells - (reply: 1)
  1365. Fixing cells with PFA - (reply: 1)
  1366. A549 cells not doubling - has anyone been through this with other cell lines? (reply: 4)
  1367. Contamination in SH-SY5Y cultures, mycoplasma? - (reply: 2)
  1368. Immunofluorescence in HEK cells issues - (reply: 1)
  1369. cell sorting - losing cells? - (reply: 3)
  1370. What if my expressed protein is toxic for the cells... - (reply: 1)
  1371. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  1372. Possible contamination of leukemic cell culture - debris, growth inhibition, cell aggregates (reply: 3)
  1373. human cancer cell line splashed into my eyes - (reply: 3)
  1374. Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
  1375. BL21(DE3)LysS competent cells - (reply: 1)
  1376. ATP turnover in neuron - ATP hydrolysis rates in neuronal cells (reply: 2)
  1377. Adhering CHO cell culture - cells don't adhere! (reply: 2)
  1378. do e.coli cells get ruptured during high spin - (reply: 1)
  1379. Please Help with COS-1 cells^^ - (reply: 1)
  1380. antibiotics for preventation of mycoplasma contamination - (reply: 4)
  1381. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
  1382. cell counting - to get an impression when my cells are in the log phase (reply: 1)
  1383. Whole cell lysis of fibroblasts - (reply: 1)
  1384. Whole cell lysis of fibroblasts - (reply: 1)
  1385. test of cytotoxic effect of compounds - why cells are pretreated with actinomycin C ?? (reply: 1)
  1386. Proliferation Assay - Should cells be in the logarithmic phase ? (reply: 3)
  1387. cell density and growth - (reply: 5)
  1388. Laser capture microdissection - capturing single cells (reply: 2)
  1389. when cells have to be synchronized?? - (reply: 2)
  1390. cells in RNAlater - the title say`s it all :) (reply: 4)
  1391. Cytotoxicity assay for adherent cells exposed to particles - (reply: 1)
  1392. Murine Cell Lines - (reply: 2)
  1393. Cell Proliferation: To adhere or not to adhere (before treatment), that is the q - (reply: 2)
  1394. why are some cell lines more popular than others?? - (reply: 4)
  1395. NK cell staining - (reply: 4)
  1396. cell culture media - with or w/o L-glutamine? (reply: 2)
  1397. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  1398. pro-apoptotic effect of compounds on cancer cell lines - which cell line to test the non-toxic effect? (reply: 3)
  1399. Expiry of Trypan blue - (reply: 3)
  1400. Question in Generating double stable cell line - (reply: 3)
  1401. activity of sex-chromosome in somatic cells - (reply: 5)
  1402. CaCl2 for making competent cells - (reply: 3)
  1403. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
  1404. ICC Cell Adherence Issue - (reply: 1)
  1405. gentamycin resistance in human cencer cell culture - please suggest the alternative antibiotic (reply: 2)
  1406. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
  1407. How to normalize my samples before I transfect my cells - (reply: 6)
  1408. Isolation of intestinal epithelial cells - Isolation of intestinal epithelial cells (reply: 2)
  1409. Number of proteins expressed in cell - (reply: 2)
  1410. Do HEK 293 cells contain functional E1A/E1B? - (reply: 2)
  1411. Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
  1412. Calcium phosphate method vs lipofectamine - troublemaker neuroblastoma cells (reply: 7)
  1413. Which cell lines are best for transient transgene expression? - (reply: 3)
  1414. Alzheimer's cell model - (reply: 7)
  1415. Partial cell subculture? - (reply: 2)
  1416. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  1417. Whole Kidney primary cell culture - (reply: 1)
  1418. dh5 alpha competent cells? - (reply: 2)
  1419. which is the best lysis buffer for whole cell extracts? - (reply: 1)
  1420. Please help me to understand- cell counting - (reply: 1)
  1421. Stimulating hepatocytes with IL-1beta - (reply: 1)
  1422. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  1423. Cell count - Please help - (reply: 11)
  1424. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
  1425. Cell Count - (reply: 1)
  1426. Generating stable cell line - (reply: 2)
  1427. Harvesting cells off of collagen gels - (reply: 1)
  1428. cells can't be lysed after crosslinking - (reply: 4)
  1429. How many cells to seed? - simple question :) (reply: 5)
  1430. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
  1431. mouse macrophage cell line - (reply: 5)
  1432. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
  1433. cell line information - require (reply: 1)
  1434. Urgent problem - Media change = cells die in 48h (reply: 3)
  1435. recombineering for ES cell targeting - retrieval problems (reply: 1)
  1436. Rat monocyte cell line - (reply: 1)
  1437. How often do you thaw new cells? - passage number or time? (reply: 7)
  1438. Does high speed centrifugation destroy viable cells? - (reply: 2)
  1439. Cell culture - (reply: 7)
  1440. Problems when plating neuroblastoma cells - (reply: 3)
  1441. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
  1442. Cell Doubling Time - (reply: 3)
  1443. Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
  1444. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
  1445. synchronized cells or non-synchronized cells - (reply: 3)
  1446. pre-prepararation of competent cells - (reply: 3)
  1447. ROS accumulation - ROS reactive oxygen species accumulation in cells (reply: 3)
  1448. Bacterial invasion assay with human cells - (reply: 2)
  1449. Measuring gene expression across cell lines - (reply: 2)
  1450. trypan blue without hemocytometer? - (reply: 2)
  1451. How to make an isotonic solution for h9c2 cells - (reply: 2)
  1452. migration < invasion??? - More cells migrating through matrigel than through control inserts (reply: 1)
  1453. Zeocin of G418. Which is the better selection agent for CHO cells? - (reply: 1)
  1454. Immunofluorescence with transfected cells - (reply: 3)
  1455. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  1456. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
  1457. Contamination in cell cultures - (reply: 9)
  1458. DMSO killing more cells at lower concentrations - (reply: 1)
  1459. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  1460. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  1461. lysing cells on ice - (reply: 2)
  1462. Fibroblast cell culture - (reply: 1)
  1463. Cardiac stem cells - (reply: 3)
  1464. How to visualize tumor cells in mice with out sacrificing the animals - (reply: 3)
  1465. cell count and dilution - (reply: 1)
  1466. z competent cells - (reply: 2)
  1467. immunofluorescence staining of sperm cells - (reply: 2)
  1468. Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
  1469. colony formation assay - how many cells to seed (reply: 2)
  1470. Primaria/gelatin plates and gravity-defying fixed cells - (reply: 2)
  1471. Contaminations in cells cultured in suspension - (reply: 2)
  1472. suspension cell transient transfection - (reply: 2)
  1473. What is this ? - Cell culture contaminant (reply: 2)
  1474. cell cryopreservation after siRNA transfection - (reply: 1)
  1475. Cell Digestion Protocol - (reply: 1)
  1476. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
  1477. cell growth - (reply: 4)
  1478. primary cells could not attch to regular polystyrene flasks but immortalised cel - does anybody know what could be happening (reply: 1)
  1479. How to culture cells after sorting? - (reply: 3)
  1480. How to kill nerve cells in a living hydrozoans? - Trying to procude partial/complete nerveless jellyfish (reply: 3)
  1481. lifespan of primary cells in vitro - how long can different cell types survive (reply: 3)
  1482. measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
  1483. T cell differentiation protocols - Th1/Th2/Treg/Th17 (reply: 4)
  1484. Mitochondria extraction from human liver cells - (reply: 1)
  1485. lipofectamine and PEI transfection of hela cells - (reply: 2)
  1486. What can I do whit my Cancer Stem Cells?(I know, its a big question..) - (reply: 7)
  1487. Wash L929 Cells with DPBS (with or w/out CA MG?) - DPBS with or W/out CA,MG? (reply: 2)
  1488. Especially tenacious cells -- what to do? - (reply: 9)
  1489. MDCK cell vascuolation - I got my MDCK cell growing in the T-75 flask (reply: 5)
  1490. Culturing HeLa Cells - (reply: 2)
  1491. expression of His-tagged protein in insect cells - (reply: 2)
  1492. How to isolate vascular smooth muscle cell? - (reply: 1)
  1493. Whole cell lysate including nucelar proteins? - (reply: 3)
  1494. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1495. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
  1496. culturing MCF-7 cellls - clumping and maintaining healthy cells (reply: 3)
  1497. Cell culture media - require information on media (reply: 3)
  1498. how to measure ROS in cell wiht H2DCFH - (reply: 4)
  1499. VEGF in cell nucleus - (reply: 2)
  1500. cell treatment rules - (reply: 4)
  1501. method to seed cells in hyrogels - cell seed in hydrogels (reply: 1)
  1502. transfecting human mesynchemal stem cells - (reply: 5)
  1503. cell schock - (reply: 2)
  1504. Flow cytometry of RFP and CFP expressing yeast cells - (reply: 1)
  1505. Can you store cell pellets for future nuclear extraction? - (reply: 5)
  1506. using cell lysate to run native gel? - (reply: 1)
  1507. Cell Suspension from Frozen Tissue? - (reply: 6)
  1508. looking for stromal cell marker - (reply: 1)
  1509. 'Black dots' in cell culture - (reply: 2)
  1510. ChIP in WT and KO cells - how to analyze - (reply: 1)
  1511. Mouse T cell line - (reply: 1)
  1512. Making competent cell from freeze-thawed stock - (reply: 2)
  1513. Invasion assay using matrigel- cell clumps - (reply: 1)
  1514. producing cell lines - why viruses ?? (reply: 4)
  1515. What color should the cell pellets be after sonication and centrifugation? - (reply: 1)
  1516. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
  1517. Mycoplasma Question - (reply: 2)
  1518. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
  1519. How to detach an antibody from cell surface - (reply: 4)
  1520. Water for electrocompetent cells - (reply: 2)
  1521. Gp 293 Luc cells - (reply: 1)
  1522. how to check cell viability - (reply: 1)
  1523. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  1524. cell fixation - (reply: 1)
  1525. cell detachment in IHC - can I incubate cells with hoechst, heparin and PFA at the same time? (reply: 1)
  1526. cell immobilization for confocal - (reply: 1)
  1527. problem with 293T cell co-transfection - (reply: 5)
  1528. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  1529. Cell Death after formaldehyde treatment - Immunofluorescence (reply: 5)
  1530. Cell Densitometry - (reply: 1)
  1531. My cells died after changing media... - (reply: 6)
  1532. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
  1533. H9C2 cells - trouble when culturing H9C2 cells (reply: 4)
  1534. Contamination of cell ?? - (reply: 2)
  1535. Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
  1536. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  1537. Passaging Cells - (reply: 4)
  1538. Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
  1539. Mysterious cell death - need Hela Help! - (reply: 1)
  1540. NSC-34 Cells sloughing off at media change - (reply: 4)
  1541. Chosing the right competent cells - (reply: 1)
  1542. Cell proliferation assay for LNCaP cells - (reply: 4)
  1543. liquid nitrogen in cell culture - (reply: 2)
  1544. differential cell counting in mixed cultures - (reply: 1)
  1545. Questions about animal cell cultue Please answer - (reply: 1)
  1546. immunofluorescence for HUVEC - how to do immunofluorescence for cancer cells (reply: 3)
  1547. membrane type of cell culture filtration - (reply: 3)
  1548. Critical column of media above cells for access of oxygen - (reply: 1)
  1549. EDTA bacterial cell lysate - (reply: 2)
  1550. Is MTS Assay suitable for Adherent cells? - Confused about MTT Assay and MTS assay.. (reply: 1)
  1551. membrane damaged = cell death? - (reply: 2)
  1552. 293 cells help! - (reply: 1)
  1553. stable cell line - (reply: 2)
  1554. Color of cryopreserved cell line - (reply: 1)
  1555. Established (tumor) cell line - or not...? - (reply: 3)
  1556. Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 1)
  1557. proliferation assay for small cell population - (reply: 2)
  1558. Cell fixation with glutaraldehyde - (reply: 2)
  1559. Yeast contamination in cell culture - hood to blame? - (reply: 6)
  1560. Comparing cell # or DNA content - of pelleted cells (reply: 1)
  1561. bicarbonate in cell culture: powder v. liquid? - (reply: 3)
  1562. cell adhesion assay - collagen (reply: 1)
  1563. To break cell clumps - suspension culture related (reply: 4)
  1564. Problems for MTT in SH-SY5Y cell line - (reply: 1)
  1565. cell stainer size - (reply: 2)
  1566. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
  1567. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
  1568. Low concentration of FBS inhibiting trypsin? - (reply: 1)
  1569. contamination in establishing stable cell lines - (reply: 1)
  1570. How much cell medium for 1 week of culturing? - (reply: 1)
  1571. p53 +/- matched-pair tumor cell lines? - (reply: 2)
  1572. Tranfection cell density and overgrowth - (reply: 3)
  1573. shRNA selection in suspension cell culture - (reply: 6)
  1574. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  1575. Ethanol/methanol/dmso for cell culture - (reply: 1)
  1576. stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
  1577. Rapid Cell Count (in vivo) - (reply: 5)
  1578. cell migration assay (corning transwell) - (reply: 2)
  1579. cell culture in 2 stack flask - (reply: 1)
  1580. Invitogen cell countess - (reply: 5)
  1581. Hypoxia Treatment For Cell Culture - (reply: 4)
  1582. Picking strain for e coli competent cells - (reply: 6)
  1583. cell death - can anybody tell me what process this looks like (reply: 3)
  1584. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
  1585. Marker do discriminate cell cycle phases - G2 phase / Mitosis (reply: 3)
  1586. hematopoietic cell lines - (reply: 3)
  1587. Cos7 Cells to produce retrovirus - (reply: 2)
  1588. how to make nonadhensive cells attach to coverslips without affecting cellular s - (reply: 1)
  1589. Constructing puromycin kill curve - How to count the number of adherent cells without detaching them? (reply: 3)
  1590. stable cell line generation - (reply: 5)
  1591. histological staining for plant cell wall components - (reply: 1)
  1592. My adherent cells are detaching - (reply: 2)
  1593. Cell culture media - DMEM and DMEM/F-12 (reply: 1)
  1594. Cell Cycle analysis of proliferating lymphocytes - (reply: 1)
  1595. Problems with HEK293 cells in 96 well plate format - 293 cells do not adhere well to the plate (reply: 1)
  1596. Cell Synchronization - (reply: 1)
  1597. Slow cell culture growth? - (reply: 3)
  1598. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
  1599. cell lysis buffer - (reply: 1)
  1600. cell aging - cell culture (reply: 2)
  1601. cell concentrations - (reply: 4)
  1602. Floating cells...pls help - (reply: 6)
  1603. Cell migration assay with 3 cell types - (reply: 1)
  1604. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
  1605. How many B cells do I need to get a 1mg of protien - (reply: 2)
  1606. Do histology staningings work in cell culture? - (reply: 2)
  1607. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
  1608. Trypsin Activity Assay - No Change (reply: 4)
  1609. T cell transduction - (reply: 3)
  1610. compound that block DNA replication in human cell - (reply: 1)
  1611. Tranfection of HL 60 cells - (reply: 1)
  1612. mouse liver cell line? - (reply: 1)
  1613. Failed Transformation of Tuner (DE3) cells - (reply: 1)
  1614. Cells suddenly died after changing the medium... why? - (reply: 4)
  1615. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
  1616. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
  1617. CHO Cell contamination - (reply: 6)
  1618. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  1619. seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 1)
  1620. Neuronal precursor cells - (reply: 1)
  1621. Sticky cells - (reply: 2)
  1622. Having problem establishing stable cell line...... - (reply: 5)
  1623. Transferring mamallian cell colonies for selection - (reply: 2)
  1624. Antibody titration and Cell number - (reply: 1)
  1625. Cell Invasion Assay - possible to analyze with flow cytometry? (reply: 5)
  1626. Cell culture contamination - "outsider I.D." (reply: 2)
  1627. Alternative media for PC-12 cells - (reply: 2)
  1628. viability of adherent cells - (reply: 3)
  1629. How long can viably frozen cells stay good in -80 degree? - (reply: 5)
  1630. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
  1631. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
  1632. HeLa cells and BCL 2 expression - (reply: 1)
  1633. B104 cells - (reply: 2)
  1634. SH-SYS5 cell culture for dummies... - (reply: 4)
  1635. collagen staining in cell culture - (reply: 5)
  1636. Hematopoietic lineage depletion kit - Hematopoietic stem cell enrichment kits (reply: 4)
  1637. changing cell shape - (reply: 2)
  1638. how to change cell shape? - (reply: 3)
  1639. About T-antigen and tumor cell lines - (reply: 2)
  1640. Sub-culture of N2a cells - (reply: 1)
  1641. Haematopoietic stem cells - (reply: 5)
  1642. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
  1643. proteomics - minimum cell number (reply: 1)
  1644. Standard Curve for OD Turbidity vs Cell density - (reply: 6)
  1645. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
  1646. Plasmids for protein expression in mammalian cells - (reply: 2)
  1647. cell lysis after induction - (reply: 1)
  1648. competent cell preparation? - (reply: 1)
  1649. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  1650. Dendritic cell infection - (reply: 2)
  1651. Competent Cells - (reply: 5)
  1652. looking for bovine skeletal muscle cells - (reply: 5)
  1653. my cell line doesnt change the colour of the media - even when cells are 100 % confluent (reply: 4)
  1654. Dead Dendritic cells ... please help - (reply: 1)
  1655. Cell concentration Question - (reply: 3)
  1656. cho cell line adoptation to serum free medium - (reply: 1)
  1657. Problems with viability of Jurkat's cells - (reply: 3)
  1658. THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells (reply: 1)
  1659. 24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
  1660. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  1661. Culturing U2OS cells - (reply: 1)
  1662. staph cell count - (reply: 5)
  1663. What kind of speed for HepG2 cells. - (reply: 2)
  1664. Serum free culture media 293T cells? - (reply: 1)
  1665. Embryoid bodies - How to differentiate mouse ES cells quickly and efficiently (reply: 8)
  1666. cell count - is it right? (reply: 3)
  1667. Haemocytometer cell count - Coverslips (reply: 4)
  1668. Growing two cell types in the same flask?? - (reply: 1)
  1669. Cell isolation - (reply: 1)
  1670. trouble to grow cells on top of coverslip - (reply: 6)
  1671. Immortalized murine hepatocytes - (reply: 1)
  1672. about measuring luciferase activity in living cells - (reply: 8)
  1673. Culture conditions for cell treatments - (reply: 4)
  1674. preadipocyte 3T3-L1 cells question? - (reply: 1)
  1675. SH-SY5Y cell culture - Suspended cells (reply: 4)
  1676. Magnetic-activated cell sorting ? - (reply: 4)
  1677. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
  1678. How to increase IF signal in acetone/methanol fixed cells? - (reply: 5)
  1679. Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
  1680. sheep red blood cell rosetting protocol - (reply: 1)
  1681. Tranformation cell harvesting - (reply: 5)
  1682. self-renewal Vs proliferation - stem cells (reply: 3)
  1683. Plyse cell and DH5a - (reply: 1)
  1684. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  1685. INS-1 cell line - (reply: 1)
  1686. Central cell seeding - (reply: 4)
  1687. please help with Freezing primary cells - (reply: 1)
  1688. COMPETENT CELL PREPARATION - (reply: 6)
  1689. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  1690. suggest cells expressing low levels of adhesion molecules - (reply: 1)
  1691. Cell Culture and Pipette Tips - (reply: 4)
  1692. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  1693. Cell counting issues - the ring of death on my flasks (reply: 5)
  1694. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  1695. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
  1696. RNA Isolation - RNA isolation from human cell lines (reply: 2)
  1697. Cell Fixation - methanol fixation of cells (reply: 3)
  1698. How to retrieve my human blood immune cells from liquid nitrogen without killing - (reply: 3)
  1699. stem cells and EB (Embryoid bodies) - (reply: 1)
  1700. Immunohistchemistry of red blood cells - (reply: 1)
  1701. cell cryosection - (reply: 5)
  1702. Shifting of entire cell cycle to the left - (reply: 3)
  1703. freezing suspended cells - (reply: 7)
  1704. Cell Brakeage...how will I know - I need the mitochondria. (reply: 1)
  1705. How many cells... - ...gives you how much RNA? (reply: 2)
  1706. Cell culture - Culture medium (reply: 1)
  1707. Anybody ever use whole blood plasma serum stimulate cells? - (reply: 2)
  1708. How can I separate my cell line from feeder cells - (reply: 3)
  1709. ChIP-seq: Minimum number of cells - (reply: 3)
  1710. CHO or VERO? - Cell culture (reply: 2)
  1711. Media for squamous cells - (reply: 3)
  1712. how to trace single cell movement throughout gastrulation of zebrafish - (reply: 4)
  1713. Oxygen diffusion through cell culture medium - (reply: 2)
  1714. Request for Mono Mac-1 cell line - (reply: 1)
  1715. help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
  1716. How much sonication power needed? - protein extraction from cells (reply: 2)
  1717. protein extraction - from cell line in T25 flask (reply: 5)
  1718. Getting cells from flasks - (reply: 5)
  1719. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  1720. Fixation of immune cells - "how to" protocol needed (reply: 2)
  1721. cell cycle analysis by FACS - (reply: 2)
  1722. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  1723. Trypsin Expression and Purification - (reply: 3)
  1724. cell cycle analysis PI staining shift - (reply: 1)
  1725. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
  1726. Problem with stable overexpression cell lines - (reply: 3)
  1727. seeking help in stable cell line generation - (reply: 3)
  1728. SDS, Cell numbers, dilution - (reply: 18)
  1729. Help with Hep G2 cells - (reply: 1)
  1730. Floating cells in adherent VERO culture - contamination? (reply: 2)
  1731. RAW264.7 cells+nitric oxide determination - (reply: 13)
  1732. BL 21 cells - (reply: 4)
  1733. Mutated MDCK cells - protocol for mutating MDCK cells (reply: 1)
  1734. Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
  1735. Cell pellet pesuspension without Vortexing or pippeting - seeking advice - (reply: 2)
  1736. western blot problem: cell culture VS mouse tissue - (reply: 4)
  1737. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 2)
  1738. Detachment of very adherent cells for flow cytometry - (reply: 7)
  1739. THP-1-Blue-CD14 cells - (reply: 4)
  1740. are cells prone to swelling in PBS after sorting - (reply: 3)
  1741. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
  1742. suspension cell culture - optimum cell number in plates (reply: 2)
  1743. Whole tissue starting product - not cultured cells - (reply: 2)
  1744. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
  1745. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  1746. Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
  1747. culturing COS-7 cells - (reply: 5)
  1748. Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
  1749. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  1750. Loss of cell adherence after transfection - The first passage after selection process cells lost adherence (reply: 2)
  1751. how will i be able to culture tumor cells? - (reply: 3)
  1752. General cell lysis question - whole cell lysates need to be centrifuged? (reply: 2)
  1753. Rnase for cell cycle - (reply: 5)
  1754. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
  1755. cell loss upon centrifugatino - (reply: 10)
  1756. cell lines vs tissue expression - (reply: 1)
  1757. Is molecular biology grade DMSO good enough for cell stock? - (reply: 2)
  1758. Clonogenic assay with agressive cell line - (reply: 4)
  1759. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  1760. Number of cells in 6 wells-plate well - (reply: 1)
  1761. protein extraction from primary cells - (reply: 1)
  1762. Extracting proteins from cells - (reply: 1)
  1763. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
  1764. strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 6)
  1765. cell floating in 96-well plate - (reply: 1)
  1766. Inducing Adherence in Suspension Cells - (reply: 1)
  1767. Isolated nuclei and trypan blue - Silly question .......... (reply: 2)
  1768. bacterial contamination in cell culture - (reply: 3)
  1769. THP-1 cell culture - (reply: 1)
  1770. H9c2 cell contamination? - (reply: 4)
  1771. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  1772. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
  1773. What stains red in trypan blue - (reply: 2)
  1774. transfection in floating cells - (reply: 5)
  1775. immortal or stable cells - primary human cells to immortal cell line? (reply: 3)
  1776. SDS-PAGE Cell lysis problems - (reply: 3)
  1777. Storage of invitrogen top10 cells - (reply: 11)
  1778. oxidative stress - how to measure oxidative stress in cells (reply: 2)
  1779. Cells clots forming during preparation mouse splenocytes - (reply: 4)
  1780. Transwell Cell Migration/Invasion Assay - (reply: 4)
  1781. neuronal nitric oxide synthase/cell line - (reply: 3)
  1782. HL 60 cells passage - (reply: 1)
  1783. Establishing a new cell line - (reply: 1)
  1784. Isolating Natural Killer cells from whole blood - (reply: 2)
  1785. Centrifugation of epithelial cells - (reply: 2)
  1786. cell engineering exam - (reply: 5)
  1787. Cell detachment: alternative to trypsine/EDTA? - (reply: 3)
  1788. Cell supplier - (reply: 2)
  1789. Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
  1790. Mir34a positive control cells - (reply: 4)
  1791. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
  1792. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
  1793. FACS positive cells in negative controls help - (reply: 1)
  1794. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
  1795. How to separate fungus cells from human lung tissues - (reply: 7)
  1796. Growing of TOP10 chemically competent cells - (reply: 4)
  1797. Stable Cell Selection with Neomycin - (reply: 4)
  1798. endogenous miRNA of cos-7 cell - (reply: 2)
  1799. Qustion about MDA-MB-157 cells culture - (reply: 4)
  1800. Folic acid solution for cell culture - (reply: 2)
  1801. Mycoplasma contamination - (reply: 6)
  1802. Strange dots around the cell+Photo - (reply: 6)
  1803. FACS of GFP expressing cells from mice brains - (reply: 1)
  1804. cells can't attach in the flasks - (reply: 4)
  1805. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  1806. Cell fractionation and isolation of nuclei - (reply: 1)
  1807. Methanol toxicity in cell culture - (reply: 15)
  1808. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
  1809. Freezing cells, quick question - (reply: 1)
  1810. Best place to buy cells - (reply: 2)
  1811. help on generation of stable cell lines with pBabe vector - pBabe-puro, phoenix Ampho cells (reply: 7)
  1812. DMSO usage in cell culture - (reply: 9)
  1813. stopping brief cell treatments - (reply: 2)
  1814. Phenol red in media - What metabolic products of cells are acidic? (reply: 2)
  1815. Cell culture container - (reply: 2)
  1816. cell lysis method - (reply: 1)
  1817. Dendritic cell immunology - activated naive cells? (reply: 9)
  1818. cell contamination???? - (reply: 1)
  1819. counting number of cells - (reply: 6)
  1820. Stacking of RAW 264.7 cells - (reply: 1)
  1821. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
  1822. Lentiviral transduction of B cells - (reply: 7)
  1823. Lentivirus transduction of spleen cells - (reply: 2)
  1824. trouble with establishing a stable cell line - (reply: 3)
  1825. Cell culture conditions and antigen expression - (reply: 1)
  1826. Phoenix packaging cell and diphtheria toxin - (reply: 3)
  1827. Gene expression in cell line - (reply: 1)
  1828. isoflurane and FACS analysis of peripheral blood? - my cells look terrible everytime I use isoflurane to anaesthetize (reply: 5)
  1829. Growing Up Cells - (reply: 3)
  1830. protein extraction after cell lysing - (reply: 2)
  1831. cell line for neurodevelopment - (reply: 2)
  1832. Difficulty in doing hemocytometer cell count - (reply: 4)
  1833. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
  1834. Method for lysing cells for fractionation - (reply: 4)
  1835. Neuronal cell culture - why use neonatal brains? - (reply: 1)
  1836. Detect gene in stable cell line, - (reply: 4)
  1837. staining cells - (reply: 3)
  1838. pIRESneo for mammalian cells transfection - (reply: 4)
  1839. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  1840. H9C2 - cell line - Need clarification (reply: 1)
  1841. Cell plating calculation !! - Help needed with Cell plating calculation !! (reply: 2)
  1842. Cell lines to study epigenetics - (reply: 2)
  1843. How to transfect two plasmids in a cell - (reply: 10)
  1844. Cells dying before i can get them to in vitro assay - (reply: 1)
  1845. Strange bands in western of RPE-1 cells - (reply: 1)
  1846. Semisolid Medium For HL 60 cells - (reply: 4)
  1847. Cell culture contamination (do not know what it was) - (reply: 1)
  1848. questions about cell lysis - (reply: 7)
  1849. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  1850. How to homogenize cell without disruption of nuclei? - (reply: 2)
  1851. Harvesting cells for flow cytometry - (reply: 10)
  1852. culture C6/36 insect cell lines - (reply: 1)
  1853. seeding cell densities - seeding cells at same density (reply: 1)
  1854. cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
  1855. Culturing QT6 cell line - Ideal culture conditions (reply: 2)
  1856. Calculating MOI for luciferase-transducted cells. - Would the sensitivity of a fluorescent microscope be enough to see the (reply: 3)
  1857. Picking epithelial, endothelial and neural cell lines - Please suggest best models (reply: 2)
  1858. How to fix suspencion cells for DAPI detecting under the microscope? - (reply: 6)
  1859. mycoplasma growth - (reply: 3)
  1860. primary lung epithelial cells - (reply: 1)
  1861. Generating double stable cell line - (reply: 3)
  1862. HACAT cells - very low cell viability after thawing cells (reply: 18)
  1863. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
  1864. My cells suddenly die !!! - Please help me ! why is this ? (reply: 11)
  1865. database for mutations in cancer cell lines? - (reply: 2)
  1866. Sonication Problems, Branson 450, LNCaP cells - (reply: 4)
  1867. ? Definition of Passage for suspension cells - (reply: 1)
  1868. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1869. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  1870. Chip problem: antibody or cell number?? - (reply: 6)
  1871. stable cell line - (reply: 1)
  1872. microbial cell viability - any recommendations - assays (reply: 1)
  1873. SF9 cells not adhering - (reply: 10)
  1874. HeLa cells - Growth of thawed HeLa cells (reply: 7)
  1875. Dendritic Cells and Flow Cytometry - (reply: 11)
  1876. direct cell lysate - (reply: 8)
  1877. How long can I wait after seeing HeLa cells till transfection - will it affect my Efficiency (reply: 6)
  1878. Culturing/Mounting of polarised cells for confocal microscopy - (reply: 1)
  1879. Intestinal cell culture: Protocol required - (reply: 1)
  1880. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
  1881. Carbon di oxide free cell culture - (reply: 1)
  1882. surface staining of THP-1 cell - (reply: 2)
  1883. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  1884. Cell Wall Lysis For Dummies - (reply: 4)
  1885. Tissue & Cell Fixation solutions - (reply: 3)
  1886. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1887. Treating cells with chemicals and how to dilute into medium - (reply: 5)
  1888. T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (reply: 6)
  1889. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1890. Has anyone used COS-7 cells for retina? - (reply: 1)
  1891. How to perform a growth curve? - i need to compare 3 cell lines for growth (reply: 2)
  1892. siRNA concentration in divinding-growing cells - (reply: 2)
  1893. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  1894. cell culture guideline from abcam - (reply: 5)
  1895. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  1896. problems with detecting protein expression - protein expression in cell (reply: 4)
  1897. freeze PBMC and cell sorting? - (reply: 1)
  1898. Control vehicle treatment in cells - (reply: 2)
  1899. Cancer cell culture exploded onto my face - (reply: 8)
  1900. FACS analysis of GFP expressing cells - Fix or not - (reply: 10)
  1901. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
  1902. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
  1903. Heterogeneous methylation in a same cell line - (reply: 1)
  1904. siRNA cell function examples - (reply: 1)
  1905. Good comprehensive resource for maintaining immortal cell lines? - (reply: 1)
  1906. DH5a comp cells - (reply: 5)
  1907. Trypsinized cells after fixation - (reply: 3)
  1908. Detaching Hela cells - (reply: 3)
  1909. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
  1910. cell cycle softawe - (reply: 3)
  1911. How to maintain cells at a specific apoptosis stage - (reply: 12)
  1912. Which cell line produce cytokine? - (reply: 13)
  1913. Cell Viability - (reply: 1)
  1914. Shipping of cryopreseved cell - (reply: 15)
  1915. To what extent competent cells be thawed before transformation? - (reply: 4)
  1916. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
  1917. Storing HEK293 cells at -80C - (reply: 5)
  1918. How to control cloned cDNA expression level in cells? - (reply: 8)
  1919. Asses mycoplasma contamination - (reply: 6)
  1920. RNA isolation following apoptotic cell labeling - (reply: 3)
  1921. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
  1922. Is there an optimal incubation time for cell lysis? - (reply: 7)
  1923. The way to count adherent cells in 96 well - (reply: 3)
  1924. lysing cells in LSB - (reply: 2)
  1925. Using NIH3T3 cells of look at MAPK activation by Ras - (reply: 1)
  1926. Staining for neural crest derived cells?? - (reply: 2)
  1927. Dendritic_ cells - (reply: 3)
  1928. problem to stain just one cell line in coculture - (reply: 4)
  1929. Inactivation of trypsin - (reply: 4)
  1930. Culturing stromal cells - (reply: 2)
  1931. A549 cells - (reply: 4)
  1932. Cunterstaining Trypan blue - (reply: 6)
  1933. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
  1934. Protein extraction from cell culture media - (reply: 9)
  1935. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
  1936. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
  1937. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  1938. can we prepare Copmetent cell by calicum cloride with 0.09 OD - Cp cells with 0.09 OD (reply: 3)
  1939. electroporation of HL60 cells - (reply: 1)
  1940. Intestinal epithelial cell culture - (reply: 4)
  1941. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
  1942. Easiest cell lysis protocol - no interest in preserving proteines (reply: 4)
  1943. passage number for cell line - (reply: 7)
  1944. MOCK INFECTED CELLS - (reply: 4)
  1945. cuboidal cells - (reply: 1)
  1946. Cell count using a hemocytometer - (reply: 4)
  1947. Hepatocyte culture - (reply: 4)
  1948. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
  1949. what is mock cell line? - (reply: 4)
  1950. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
  1951. How to seed the cells evenly in 6 wells plate - (reply: 13)
  1952. knockout mouse with increase cell size - (reply: 3)
  1953. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
  1954. Paper on energy requirement of cells - (reply: 2)
  1955. galactose induction of yeast cells - (reply: 2)
  1956. making competent E coli DH5a cells - (reply: 6)
  1957. How could you explain these results? - cancer cells are weird (reply: 1)
  1958. strange ES cells contamination - please help!!! (reply: 1)
  1959. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)
  1960. How to culture PC3 cell line - (reply: 3)