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protocol for making e.coli competent cells under low temperature - (Nov/01/2011 )

Hi, I have heard that e.coli chemical competent cells made under low temperature i.e below 37C are very competetive. Does any one has protocol for it?

-nainiburman21-

Here you go:http://openwetware.org/wiki/TOP10_chemically_competent_cells

-bob1-