CaMKII alpha promoter and cell tranfection - (Jun/06/2011 )
I've built a vector with the neuronal CaMKII alpha promoter and now I need a cell line to test it. I have tryed with SHS5Y cells but the transfection reagent (turbofect from fermentas) are killing them. Also lipofectamin.
how do you transfect SHSY5Y cells?
do you know any other cell line that are able to express genes driven by neuronal CamKII alpha promoter?
thanks a lot
I successfully transfected SH-SY5Y cells using a device from nanoTherics Ltd (http://www.nanotherics.com/) ~80% transfection efficiency and ~90% viability.
What is the size of your plasmid?
I have also successfully transfected Primary Hippocampal Neurons with an 11kb plasmid (Synapsin-1 promotor) with the same device!