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serumm free culture of adherent cho cells - (Jul/11/2012 )

Hi all,

i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media for 72-96h. i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions which medium i can use to sustain 72-96h time?



I use Freestyle CHO media from Gibco, shake flasks at ~120rpm.