Help with Hep G2 cells - (Jul/27/2009 )

Could anyone provide some suggestions for working with HepG2 cells? I have some cells that were frozen by a previous lab member. They are at passage 76. When I thaw them and culture according to ATCC, most of the cells are floating by the next day. I have had the cells growing for over two weeks now, and have some clumps of cells, but nothing is spreading out on the plate, and I don't think that they will ever reach confluencey. Is the passage number too high? Would I be better off ordering new cells from ATCC? Any suggestions would be greatly appreciated. Thanks!

Katie

I suspect the cells will not survive; those clumps you are seeing will likely be quite different to the parent line. You will be better off getting some more.