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Can u suggest any myoepithelial cell isolation protocol with the help of reagent - (Jul/06/2012 )

I would like to know the separation procedure of myoepithelial cells of breast with out using any magnetic beat or etc. Please advice me a protocol where I can separate these cells from epithelial and fibroblast by using any reagent or chemical.

-Priti Prasanna Maity-

one of my friends does this with antibodies and separation column. However, he works on other cell types. Why don't you want to use magnetic bead?