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After centrifuge in serum-free media, forming cell sheet, instead of pellet in t - (Jul/10/2014 )

Hi

Has anyone encounter cell sheet across vertically in the tube, rather than normal cell pellet sink to the bottom after centrifugation ? How to get the supernatant out without disturbing the cell sheet?

 

Thanks

-roxx_gurl-

Are you sure it is cells?  This is quite unusual, but I could see it happening with fat cells. 

 

You could use a hypodermic needle and a syringe to minimize the damage to the layer?

-bob1-

Oh 

 

Yes. It forming cell floated instead of cell pellet sink to the bottom after centrifuge.  I have experienced this before using human gingival epithelium (normal) from Cell N Tec and using their culture media, I thought they must have differentiated due to confluency and maybe because of serum-free media. 

 

Btw, I tried to neutralized trypLE (can stop detachment by dilution)  with serum-containing media (maintenance culture using serum-free media), and after centrifuge, they formed cell pellet at the bottom like normal. 

 

I guess the cell pellet formation due to serum-containing media, somehow serum attach cell together and help to form cell pellet sink to the bottom,easy to manage

 

What do you think?

-roxx_gurl-