How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (Feb/24/2014 )
I'm trying to figure out how I can have 2000 cells/ well.
I thought of doing it like this:
2000/0.2= 10e4 cells/ml
Lets assume I have 100 wells (easier for calculation), then 200ul*100 wells=2e4ul/20ml
So in 20ml, I would need 2e5 cells.
Yes, you will need 20mL of 10^4 cells/mL solution. 200uL of this solution per well will give you 2000 cells/well.