cells shrink, detach from each other when changing media - (Nov/30/2011 )
I am culturing a glioma cell line. I need to change the media after 2-3 days. Whene I change the medium and look back at the cells, I notice that they detach from each other clumping up and kind of shrinking into islands from a continuous monolayer.
I am adding warm DMEM, which is what they are growing in before the media exchange.
Has anyone experience the same and/or has an idea of what is going on?
I think most of the cells that sort of shrink and detach eventually die.
Any help would be great
Try taking some of the medium off the cells, filtering it and adding it at 2:1 oe 3:1 (fresh : old) back onto the cells. It may be that you are removing secreted growth factors from the cells, which they need to replenish before they will grow again.
Thanks, could a different FBS solve the problem as well?
Possibly, if you have changed batches recently, that could be the reason this has started happening. In the future, when a new batch is being brought in, get samples and test it against your cell lines (you should be doing this anyway).