Looking for protocol to do biotinlynation on each single protein in cell lysate - (May/31/2011 )

Hi,

I would like to know is anyone could provide the information about doing the biotinylation of each single protein in cell lysate.

Thanks.

I'm not sure how you could biotinylate each single protein in a cell lysate. There are comercial biotinylation reagents available to add the biotin tag to all proteins that have primary amines available. I have had reliable results with the Pierce biotin reagent sulfo NHS biotin.