How to isolate vascular smooth muscle cell? - (Feb/18/2010 )
From a primary culture of vascular smooth muscle cell, I have obtained VSMCs and other cellular types. How can I isolate only VSMCs and discard the other one? I know that the major type is smooth muscle cells because I done immunocytochemistry (alpha-actin).
Thanks!
-nis-
nis on Feb 18 2010, 04:02 AM said:
From a primary culture of vascular smooth muscle cell, I have obtained VSMCs and other cellular types. How can I isolate only VSMCs and discard the other one? I know that the major type is smooth muscle cells because I done immunocytochemistry (alpha-actin).
Thanks!
Thanks!
Live-cell FACS or magnetic bead sorting should allow you to purify your population if you have a specific marker for your cells of interest.
-gfischer-