abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (Aug/05/2010 )
After transfection with gene-of-my-interest-specific shRNA, there was a significant change in nuclear morphology.
It seems like it has something to do with maintaining normal nuclear structure, but I am not sure where to go from here with this.
I don't even know what to call this phenomenon in English. Therefore googling is not possible at the moment.
I know it is silly to show just one picture and ask other's opinion. Any short, rude, or half-minded reply might be a great help to me.
The confocal image is attached. Nucleus are colored in red and my gene is not in the frame cuz it was completely silenced.
I have checked control pool is perfectly normal.
What do you think? Why do nucleus have such a giant hole in them? It doesn't look like speckle.
Looks like it might be the nucleoli beeing altered. Otherwise it is some sort of vacuole.
Thanks for your comment, bob1! I will search for more information according to them.