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abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (Aug/05/2010 )


After transfection with gene-of-my-interest-specific shRNA, there was a significant change in nuclear morphology.

It seems like it has something to do with maintaining normal nuclear structure, but I am not sure where to go from here with this.

I don't even know what to call this phenomenon in English. Therefore googling is not possible at the moment.

I know it is silly to show just one picture and ask other's opinion. Any short, rude, or half-minded reply might be a great help to me.

The confocal image is attached. Nucleus are colored in red and my gene is not in the frame cuz it was completely silenced.

I have checked control pool is perfectly normal.

What do you think? Why do nucleus have such a giant hole in them? It doesn't look like speckle.

Attached Image


Looks like it might be the nucleoli beeing altered. Otherwise it is some sort of vacuole.


Thanks for your comment, bob1! I will search for more information according to them. :)