Selection of needle size to homogenize cells for RNA extraction - (Jun/26/2013 )

Dear All,
I used RNeasy Mini Qiagen to extract RNA from cell culture.
But if i use 26G needle instead of 20G needle (as written down in the handbook) to homogenize the cells in cell culture, will it affects the quality of RNA or i loss more RNA etc?
Help..

Edited to make font readable. Bob

I have used that red color kit, but never used any needle.

If you do not use syringe and needle, how do you homogenize your cells?
Thanks ~

you said cell culture. they don't need homogenization, unless you mean tissue cells?