Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (Jul/20/2009 )
Some kind of doubts have haunted my mind........ i have cells (HepG2) transfected (Fugene delivery system) with RNAi against Dengue virus structures. I make my selection using G418.
I made a stock of these cells line, last years using DMSO 10% Bovine serum 20% and Dmem medium. Stored at liquid nitrogen.
Recently i decide to thaw my cells from liquid nitrogen and then, my cells can't attach in the flasks. I turned to this forum on section of "tissue and cell culture". The cells are growing but they are in suspension.
Last weekend i talked to a friend about this and she wondered herself if any RNAi wouldn't be interfering (in cross reaction) with some kind of molecules of these cells and undergoing them to not attach.
Does anyone here have similar experience???
Thank you very much
It is certainly possible that the RNAi is suppressing expression of genes which encode proteins involved in substrate adhesion.
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