Housekeeper for HEK cells? - (May/17/2013 )

Hello everybody,

I am working with HEK293 cells and tried to check my RNA integration by using usual housekeepers (EF-1a, GAPDH, SDHD) but apparently they are not able to bind to this template, although in silico analyses revealed that they should. I tried them with quantitative PCR as well as with conventional RT-PCR with the same results.

I would be really happy for any suggestions.

Thanks a lot,

Best
Stephanie

That's strange. You gapdh must give a sharp band if your cdna is synthesized properly.