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Debris in cell culture - (May/17/2013 )

Hi everyone!! Recently at my lab, cells in culture (hMSCs form Lonza) have shown to have little black dots which we have described like Cell Debris (we have been reading this forum to come to this idea). My question is if these debris may signal a hidden problem. Cells seem to be OK (no death, no contamination, no Mycoplasm ...) but i'm worried about if they may start behaving differently. Do you know if debris are a sign of any problem?

Thanks to everyone!



If you can post a picture, that may help us determine if they are in fact debris or a common contaminant (see here for discussion).


It's quite common in mammalian cell culture. You can try Lymphoprep to get rid of those black dots.