Protocol Online logo
Top : New Forum Archives (2009-): : Tissue and Cell Culture

Powder indomethacin in cell culture media?? - (Aug/08/2012 )


I'm trying to induce rat mesenchymal stem cells to differentiate into adipocytes. The protocol I have in hand mentions indomethacin in the DMEM medium. However, indomethacin powder is the only thing I have and it does not dissolve in DMEM nor PBS. any ideas?


-The Dark Knight Pipettes-

Try DMSO or DMF (di methyl formamide). The product sheet should tell you the solubility in different solutions.


DMSO works like a charm. Thanks Bob.

-The Dark Knight Pipettes-