Eruption of mammalian cells using detergent - (Dec/18/2010 )
I am looking for an effective way to disrupt mammalian white blood cells (physical shearing such as a beadmill is not possible at this stage) without actually purifying the DNA... As such i am considering a mild detergent such as 0.05-0.5% Tween 20 or Triton-X and perhaps some some mild vortexing.... Do you think this will be sufficient for this purpose? Another option might be simply several freeze/thaws however i don't know how efective this will be... Any ideas or suggestions?
One freeze-thaw in a lysis buffer will be more than sufficient. It depends on your end purpose what lysis buffer to use.