RAW 264.7 cell line - (Oct/03/2013 )
I am culturing RAW 264.7 cells and recently i found cells are not growing fast and producing lot of metabolites. after i changed the culture media within 12 hrs they are producing lot of metabolites and not growing well and after 24 rs cells starting to die. cells also showing morphological changes too.
Please if anyone know the reason help me to overcome this problem. now my frozen cell store is also going to be finished.
Thank you very much
I found the solution by myself. reason is with the culture media and the pH value has changed. Therefore I prepared new culture media and used new tube of frozen cells. Now it seems to be fine. Thank u .