cell viability assay for caco-2 and pbmc - (Jun/06/2016 )

Hi. I am interested to measure the cell viability of Caco-2 and human PBMCs.

1. Have anyone tried before using MTT/MTS/ATP assays/Neutral Red to measure cell viability of Caco-2? Is MTT/MTS assays more sensitive than NR?

2. I will be growing the cells for ~1 week and stimulating the cells for ~2 days in 24-well plates. Do I have to trypsinize the cells, count cells, and seed on 96 well plate? *Do anyone have any protocol for assays with Caco-2? 

3. I read that PBMCs will produce low readings using MTT assays. Do you have any recommendations? Will negative staining with PI/7-AAD suffice?

Thanks. 

protocol for assays with Caco-2:

http://static1.1.sqspcdn.com/static/f/603808/7365406/1276716507307/Caco2+assay+protocol.pdf?token=pItuIDym4hTqHztyDDdP4XH1Ff0%3D

Cell Viability Assay Kits:

http://www.creative-proteomics.com/search.aspx?q=Cell%20Viability