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Heeeeeelp..cell cycle sample..is it possible to store it? - (Jan/19/2013 )

Dear All,
After I got my samples ready (quite large samples) for cell cycle analysis I found the FACS is not working I got very upset because I spent long time preparing these samples. Actually I didnt know what is the proper thing I should do with the samples especially because I was working in weekend so I decided to put the samples in -80 (samples labeled with PI) ....

Do you think it is possible to store labeled samples??

Please please offer me all the help you can...

Many thanks in advance

-shs-

It should be fine to store at 4 deg C if they are fixed, you may need to re-label the samples with PI though. -80 will probably cause the cells to be too damaged by ice crystal formation during the freeze/thaw process, to be used.

-bob1-

Thank you so much for ur replay. So I need to prepare the samples again :(

-shs-