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Oxygen diffusion through cell culture medium - (Aug/10/2009 )

Hi all,
I would have a question regarding oxygen diffusion through/in cell culture medium. If I have cells on plate with 2 ml of cell culture medium (surface of the well is 9.5 cm2) and then I put the plate from normoxia to hypoxia (1% of oxygen, 37C is in the hypoxia incubator), how long approximately does it take to reach 1% of oxygen in the cell culture medium (and in cells)?

Thanks.
With regards,
vic

-victor.m-

Its best to measure it experimentally because there are so many variables involved.

-genehunter-

there are equations that describe the diffusion rate in an ideal system, but as genehunter said, there are lots of variables involved.

http://en.wikipedia.org/wiki/Diffusion_equation

-bob1-