immunostaining of cells in culture - (May/03/2012 )
I have to test for the presence of stem cells in my cell line by immunohistochemical staining (IHC). I've done IHC on formalin fixed
Culture your cells on cover slips. In the well: wash with PBS, fix with 4%PFA in PBS 5min @RT. Wash with PBS +0.1% Triton X100 2x5min. Block, stain, wash, stain, wash, mount.