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DH5alpha cells - (Jul/10/2011 )

why are DH5alpha cells always preferred.. what are other common cells available for cloning.

-shrimathi ravikumar-

Hmmm, homework?

-bob1-

bob1 on Sun Jul 10 23:50:40 2011 said:


Hmmm, homework?

not exactly.. kinda interested in knowing!

-shrimathi ravikumar-

Have a look at the genotype - there are several features that make it suitable. These are in common with other strains that are frequently used.

-bob1-

bob1 on Tue Jul 12 23:36:21 2011 said:


Have a look at the genotype - there are several features that make it suitable. These are in common with other strains that are frequently used.

recA-: eliminates homologous recombination and endA1-:inactivates an intracellular endonuclease that degrades plasmid DNA in many miniprep methods

-qzlabs-