What happens when trypsin is added to cells? - (Mar/15/2015 )

Hi,

when trypsin is added to cells, the cells dissociate and loose the morphology, but I dont know what happens to the cell cycle phase. Does the cells stay in the same phase as they were in, or do they go into the Go phase. Iam trying to see in which phase of the cell cycle  my protein enters the nucleus. Right now Iam looking at the G2 phase, but not able to trap the cells in G2 phase of the cycle cycle. The cell cycle is 16-18 hrs and I dont syncronize the cells. If anyone does similar work, please help.

Jaya

Trypsin doesn't affect the cell cycle that I know of. to get most cells into G2 you will need to synchronize the cells in G0/1 and work from there.