How many cells do you have in a confluent T 75 flask? - I have 1/2 million (Mar/17/2011 )

Hello everybody!
In our lab we isolate cells from a cancer tissue, now in passage 5, but when I count the cells with a hemocytometer I count about half million of total cells in a CONFLUENT T 75! I thing is few cells (sorry for my english). When I saw the cells in a microscope, they are big , could be it a explication for the poor count?

how many cells did you count in your labs?

thanks a lot!

That is a fairly low number of cells. For a standard size cell, you should get around 1x10⁷ cells. What sort of cells are you growing out as yours seem extremely large which makes me think that they are probably entering senescence and won't grow any further?

hi

i had the same problem with melanoma cell line the cells are clamping and leading to error in counting 

we used from EDTA 0.02% to solve this problem. 

sahar