cell culture clarification - I need help please (Dec/13/2010 )
I am a chemist who has gotten roped in to doing cell culture at work. I have to purify media collected from cells and I have never done it before. I have a protocol from a journal to work with. The cells have grown and media has been collected which I now need to purify.
journal says "medium...was collected and clarified at 3000rpm in a Sorvall RT6000 refrigerated centrifuge for 10min at 4C." I do not know what is meant by "clarified" Do I use a special centrifuge tube with a filter? or am I over-analyzing this and it simply means to spin down? Or is there something I put in the centrifuge tube?
Thanks for any help
Dawn M on Mon Dec 13 19:28:01 2010 said:
In this context "clarification" means "removal of bigger debris" - eg. cells, cell fragments etc.
haha thanks! I thought so. it just seemed too easy
I was also wondering what they meant by 'adjusting the supernatant to...' but then realized they just meant added