Apoptosis via cell to cell contact - (Jan/28/2013 )

Hello,

I wish to perform an assay where I want to treat the PBMCs I got from a human subject with certain antigens and see if it will induce apoptosis of the target cells by CD8 T cells via cell:cell contact. The problem here is that I do not have the tissues/cells from the same subject I got the PBMCs from! So I assume the allogeneicity will be a big problem...

I am wondering if anyone has any suggestions on what I can do?

Thank you very much!

I think you can still proceed with relative comparison just to compare the effect on different cells. At least you won't waste your PMBCs.