Cell Debris Isolation From Blood - (Jun/02/2013 )
Does anyone have any suggestions for isolating cell debris/particles from blood without using ultracentrifugation?
Would appreciate your help and suggestions,
cell debris means?
Have you considered a density gradient medium like iodixanol? Without more details as to what you're trying to accomplish, it's difficult to give much in the way of advice.
Thanks for your reply. No I was more thinking in the direction of filteration (tandem filteration) using micro filter polymers. But iodixanol seems to be a promising way as well. I need to be able to separet the blood particles from stressed blood and study them in a microfluic system (i.e spike certain amount of these debris into intact blood and run them in a microfluidic device to find the threshold for failure). Do you have a good protocol to suggest for using iodixanol for this purpose? I would really be very grateful if you could help me out with this.