PBMC cells handling - (Nov/01/2012 )
maybe it's a silly question, but I'm not a cellular biologist and I need some help.
I isolated PBMC from the whole blood and then I seeded them in a 96 well plate. After incubation of 24h I would like to monitor the presence of a certain protein on their surface using a labelled antibody.
Do I need to treat them as adherent cells using trypsin before adding the mix with the antibody or can I resuspend them pipetting?
What do yo think?
thanks a lot!
what is your desired method of detection? there is no need to use trypsin for suspension cells.