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Growth conditions for E. coli DB3.1 cells - (Mar/28/2011 )

Hi everyone,

I am trying to grow DB3.1 cells that have been transformed with pHellsgate 12 vector. I was wondering what specialized culture media and growth conditions should I use, if any?

Please advise. Thanks!



according to Invitrogen's manual no special conditions neccessary!

in minimal medium it will need some amino acids as far as i get it from the detailed genotype ...that can be found here. But in normal LB medium everything should be fine!