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FITC conjugated phalloidin staining of cells on microcarrier beads - (May/18/2014 )

Hi,

 

          I m working on microgravity based cell culture on microcarrier beads. I m trying to visualise F actin in huvecs cultured on dextran based cytodex microcarrier beads. I tried staining the beads with FITC conjugated phalloidin. I m getting very high background from the beads. and hence I m not able visualise the cells on the beads. HAs anyone encountered such problems? Any help would be appreciated

 

 

 

Thanks :)

 

Vidhya

-vidhya-

Some details of what you have already tried would be useful.

-bob1-