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culture media for pancreatic cancer stem cells - (Sep/24/2012 )

I am planning to isolate pancreatic cancer stem cells and test on them a couple of drugs. However, i am not so sure of the exact media that I'd use to propagate the cells without differentiation. (i'll be testing the drugs in sphere formation assays & migration assays).

I know the media has to be serum-free, have growth factors: EGF & FGF-2.

Does anyone have a recipe?
thanks a lot

-mihoz-

I am planning to isolate pancreatic cancer stem cells and test on them a couple of drugs. However, i am not so sure of the exact media that I'd use to propagate the cells without differentiation. (i'll be testing the drugs in sphere formation assays & migration assays).

I know the media has to be serum-free, have growth factors: EGF & FGF-2.

Does anyone have a recipe?
thanks a lot

-mihoz-

I am planning to isolate pancreatic cancer stem cells and test on them a couple of drugs. However, i am not so sure of the exact media that I'd use to propagate the cells without differentiation. (i'll be testing the drugs in sphere formation assays & migration assays).

I know the media has to be serum-free, have growth factors: EGF & FGF-2.

Does anyone have a recipe?
thanks a lot

Please don't multipost- Bob

-mihoz-