Would someone help me discuss what work can I do with laryngeal cancer cell hep2 - (May/07/2014 )
I am new. I want to begin with laryngeal cancer cell line hep2 cells. Would someone please tell me something about it?
A quick google search will tell you quite a bit about it... it is a HeLa derivative - so not laryngeal at all. The ATCC has some good information.
I got it. Thank bob1 very much. Another question, if I could harvest laryngeal cancer cells directly from the fresh specimen, or will it be better for me to buy laryngeal cancer cells from biotech firms? Is the result better accepted with purchased cell line?
Either is acceptable, in many ways primary cells are actually a better choice than commercial lines, as establishment of a line means that it has (probably) undergone a number of changes that make it amenable to cell culture.
Having said that, primary culture can be difficult and is time consuming.
Agree with Bob and I'd go for primary culture if it's possible; in many cases primary cultures are a better reflection/approximation of the actual in vivo situation than commercial cell lines.
Thank you all! As I'm a green hand at cell cultivation, starting with commercial cell lines might be easier for me. I searched google and pubmed, and I got HLaC'79, HLaC'82, and HNO258, but with few articles.
Looking forward to further disscussion~
Check if the cell lines you're interested in are available at the ATCC http://www.lgcstandards-atcc.org/Products/Cells_and_Microorganisms/Cell_Lines.aspx?geo_country=de usually you can find basic information there such as how to cultivate them. Or do you have a specific question ?
I'm back again!
I searched http://www.lgcstandards-atcc.org and found commercial normal human airway epithelial cells. I think they can be used as a control in my future work~thank Tabaluga
I'm embarrassed to ask another question, which may be kindgarden to you all, that if I could cultivate cells in my lab then transfer them to another lab in another city? How to achieve it? Will they lose vitality after long time transportation?
Note that if you are crossing borders from one country to another you need to ensure that you have all the relevant permits for importation and exporting and biological safety. If you are transporting by air you need to follow IATA guidelines or risk a very very big fine (US$ 100,000 or more). In both cases I would strongly suggest using a registered courier company (I usually use World Couriers, but I have heard good things about FedEx) as they should be able to tell you the requirements, and handle the shipping properly.
There are two methods of transport of cell lines - as a frozen cell suspension or as a living culture in a flask.
Frozen cells can be transported on dry ice for as long as your dry ice lasts or as long as you can refill the container with dry ice. Note that dry ice is frozen CO2, so if you are transporting it yourself in a vehicle make sure that you don't get gassed as it sublimates!
Living cultures in flasks will be OK for a 2-4 days, the flask needs to be filled as full as you can get and an impermeable lid (i.e. lid with no gas diffusion filter) placed on it tightly. The flask should be shipped at room temperature and not be subject to big changes in temperature (i.e. no hot cars!).
Thank you! It seems extremely dangerous! Transporting living cultures is also difficult! Without your advice, I might have been lying in my car, with my soul talking to you all!