can i use trypsin to collect cell sample for 2d gel analysis? - (Mar/16/2011 )

can i use trypsin to collect cell line sample from plate for 2d gel proteomic analysis? would that influence much about the result of experiment?

or should i only use a "rubber police"?

thx~

you can use trypsin as long as you wash the cells thoroughly before you lyse them.

mdfenko on Wed Mar 16 19:28:38 2011 said:

u mean after collect the cell by trypsin, right?

3 time by Wash Buffer good enough?

yimao on Thu Mar 17 03:26:32 2011 said:

yes

if you drain the pellets well