Isolated nuclei and trypan blue - Silly question .......... (Jul/02/2009 )
Long time listener, first time caller!
I'm currently isolating nuclei from HeLa cells and many protocols say that they "confirm the nuclear isolation and purity using trypan blue". I have used trypan in viability assays in the past to count blue/pink cells, however, what should isolated nuclei look like under trypan and how would they look different from whole cells?
They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes.
bob1 on Jul 3 2009, 01:14 AM said:
Thanks for that! I'll take a look ..........