help Splashed Raji cells on skin/face/hand - (Jan/25/2011 )
I had a general question. I had some raji cells plated in a 96well plate. I had spun them down gently to get a pellet ( at 100g). When I was taking the plate out of the centrifuge, the centrifuge door slammed down on my arm causing some of the media to splash on my wrist and even a few drops on my face. I don't think any got in my eyes, at least I didn't feel anything. Obviously I washed and rinsed my hands and face with soap and water and let water run on me for a minute or two. Normally I wouldn't be concerned, and yes I know that my immune system should take care of it, but the thing is I'm pregnant. I know that a woman's immune system is compromised during pregnancy, but to what extent I don't know.
I have to say that maybe 100 ul of media splashed on me, and it may or may not have contained some raji cells in there. As I said earlier, I had spun them down a bit, but I'm sure there were still some cells floating around somewhere.
Do you think I'll be ok even with a slightly compromised immune system? I don't want to freak out or panic, but I do want to take caution. I did have gloves, but as I said it was on my wrist and face.
These are just my 2 cents, but I think that in the absence of all the factors and enough humidity they will just die on you.
You took proper precautions (and you didn't mention open wounds...)
You will be fine, the cells do not survive well outside a culture environment and you took the appropriate actions in washing yourself thoroughly.