determining optimal cell count for MTT - (Sep/21/2011 )
I'm new to the MTT assay and from what I've read, I have to first determine the optimal cell count before performing the assay with my test sample. None of the protocols that I've read for this
With the MTT assay, the viable cells convert the substrate into formazan crystals. Therefore, the toxic agent is not needed for establishing optial cell nubmer. You want to make sure you are within the linear range with your cells and timepoints. Please feel free to look over the manual for the Promega version for more information: http://www.promega.com/~/media/Files/Resources/Protocols/Technical%20Bulletins/0/CellTiter%2096%20Non-Radioactive%20Cell%20Proliferation%20Assay%20Protocol.ashx
Also, please do not hesitate to contact Promega Technical Services with any questions you may have.
O right! I'm sorry my bad.. i didn't quite read the principle properly and therefore misunderstood how the assay works.. Thanks Kevin