[Question] How to remove cell debris stuck on the attached cells in primary card - (Aug/09/2012 )

Dear all,

I encounter a problem of clung cells/debris while doing rat cardiomyocyte culture. After 2 days of seeding, using DMEM, I tap the dish hard to wash away tissue/cell debris. However, even after more than 5 times of wash, I can still find some cell clumps are very sticky and difficult to remove. Clinging on the beating cardiomyocytes and piling up high, these clumps cause a great trouble for me to take clear images. A picture is attached for your reference.
Is there any way to remove these masses and leave the seeded and attached cardiomyocytes not affected? Thank you very much.

You could use a P200 and suck the clumps away individually

leelee on Fri Aug 10 03:18:22 2012 said:

In addition to the larger clump, many are singles cells that instead of adhere to the well but landed on other cells and stick there. They can be seen only under a microscope.