cell cryopreservation after siRNA transfection - (Mar/04/2010 )
I need to do live cell imaging after cells been treated with different combinations of siRNA transfection, the problem is that we only have one microscope and can do only one type of cell at a time. I wonder if I can freeze the siRNA transfected cells and do the imaging separately? what does freezing do to the gene silencing level of the before siRNA transfected cells? has anyone done this before? please link me to any references possible, THANKS!
The freezing process is quite traumatic for cells. I would not freeze my cells unless I could prove that the freezing did not affect the siRNA activity or alter expression of the genes I was looking at.
Just do your transfections sequentially, it'll save you a lot of time in the end.