Problem in Cell Viewing - (Feb/06/2014 )
I was using Anthony's staining method to view the encapsulated bacterial cells. I have a question that would like to seek for your advice. Attached is the cells image after staining with crystal violet.
First image (IMG_6985) is the bacterial cells incubated with maltodextrin and fructo-oligosaccharide. Did anyone know what is that pinkish stuff? Is maltodextrin or fructo-oligosaccharide? Or the combination of both of them?
Second image (IMG_7007) is the bacterial cells incubated with maltodextrin only. I noticed there is a number of sphere shape capsule-like compound. Is that the encapsulated bacterial cells?
Magnification? Is bright field microscopy?
The second looks more like bubble-like stuff but no cells.
Magnification is 100x, under light microscope. Yup. I was suspecting the bubble like stuff is actually maltodextrin, do you think there are cells being encapsulated inside the bubble-like stuff which not able to stained out using crystal violet? Thanks.
Hard to say without a reference of a naked cell