is -40 freezer enough for cell freezing? - (Feb/03/2015 )

I have routinely used the standard IPA container in -80 overnight for cell freezing before transferring it into liquid nitrogen. But at the new place I have joined, we have only a -40 feeder and no -80 freezer. Can I replace -40 with -80 during freezing before liquid nitrogen step? How important it is to take cells to -80 before immersing in liquid nitrogen?

Thanks

Yes, you can transfer from -40 to LN2 without problem. I have done it several times with several cell lines, out of impatience, so I am sure it works.

However, don't do it with some sensitive or irreplaceable cell line without doing some trial runs. And if you do, don't sue me. What's with your Avatar name by the way?