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cell cycle arrest by colcemid - (Oct/08/2010 )

I stain my cells with CFSE to analyze their cell cycle activity and as a positive control, I would like to culture the cells under the presence of colcemid which inhibts the cell cycle
I cannot find any information on the stability of colcemid in culture (37, pH 7.4) . How often do I have to refresh the colcemid-medium, so that cell cycle is efficiently inhibited?

-ija0-

I've never used Colcemid, but I use Mitomycin C in medium. Could you use it as an equivalent?


ija0 on Fri Oct 8 09:52:01 2010 said:


I stain my cells with CFSE to analyze their cell cycle activity and as a positive control, I would like to culture the cells under the presence of colcemid which inhibts the cell cycle
I cannot find any information on the stability of colcemid in culture (37, pH 7.4) . How often do I have to refresh the colcemid-medium, so that cell cycle is efficiently inhibited?

-aleruiz-

ija0 on Fri Oct 8 09:52:01 2010 said:


I stain my cells with CFSE to analyze their cell cycle activity

???? Cell cycle by CFSE? :)
May be "to analyse cell divisions"?

-Denis Baev-