hematopoietic cell lines - (Nov/19/2009 )

Hi to all,

Anyone here has experience with hematopietic cell line expansion? I am about to buy one, but I do not know which is good..does any one has recommendation?

Past few months, i cultured cord blood hematopoietic stem cells. The problems: cells didnt expand much after 1-2 weeks upon expansion and couldnt be maintain in its undifferentiated stated. I used serum-free media and cytokine cocktails from stemcell technologies. So I was thinking, why not I try with the cell line.

Thanks in advanve for any responses..

Rgrds,
Boudou

-boudou_611-

boudou_611 on Nov 20 2009, 12:23 AM said:

Unless you use a system that supports continous perfusion with fresh media, the cells in culture are going to "talk" to each other. This paracrine signalling causes stem cells to head down a differentiation pathway as you have experienced.

I am not aware of any commercial system that fixes this problem other than the Aastrom system, which they no longer sell for laboratory use (and that one was lots of $$$$).

-Hodag-

I'm looking at mouse haematopoietic stem cells. Does anyone else have experience with a good cell line?

-Piersgb-

Hi Piersgb,

The "gold standard" for proving that a cell derived from mouse bone marrow is indeed an HSC is still based on the same proof described above and used in mice many years ago. That is, the cells are injected into a mouse that has received a dose of irradiation sufficient to kill its own blood-producing cells. If the mouse recovers and all types of blood cells reappear, the transplanted cells are deemed to have included stem cells.

DNA Methyl transferase 3B

-StephenJones-